OBJECTIVE:To take Baihui(GV20)and Fengchi(GB20)targeting inflammatory response to regulate migraine as an example to describe a new method for studying the mechanism of stimulating acupoints.METHODS:The target informa...OBJECTIVE:To take Baihui(GV20)and Fengchi(GB20)targeting inflammatory response to regulate migraine as an example to describe a new method for studying the mechanism of stimulating acupoints.METHODS:The target information of Baihui(GV20)and Fengchi(GB20)was retrieved,and after intersection with migraine,Kyoto Encyclopedia of Genes and Genomes(KEGG),Reactome,and Uni Prot Keywords were used for functional enrichment.After selecting the main pathway,rats were selected and nitroglycerin was used for modeling,and the behavioral scores,inflammatory factors,heme oxygenase 1(HMOX1),protein kinase B(AKT1),signal transducer and activator of transcription 3(STAT3),phosphorylated extracellular signal-regulated kinase 1/2(P-ERK1/ERK2)and other states of the rats in the acupuncture,twisting,and electroacupuncture groups were compared.RESULTS:A total of 135 Baihui(GV20)targets and 27 Fengchi(GB20)targets were collected.A total of 73 target information were obtained after the intersection of these targets in migraine.These 73 targets have three main pathways:hypoxia-inducible factor 1(HIF-1)signaling pathway,signaling by interleukins and inflammatory response.The main targets in the pathway were verified and found that interleukin-1 beta(IL-1β),interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α)and HMOX1,AKT1,STAT3,P-ERK1/ERK2 can be regulated by Baihui(GV20)and Fengchi(GB20).CONCLUSION:Baihui(GV20)and Fengchi(GB20)can regulate migraine by regulating inflammatory factors and HMOX1,AKT1,STAT3,P-ERK1/ERK2 and other changes in HIF-1 signaling pathway,Signaling by Interleukins and Inflammatory response pathways.Based on systems biology and network pharmacology,and with the model of"acupoint-target-disease",explore the research methods of systematic acupuncture and moxibustion.We believe this is a usable research direction for exploring the mechanism of acupuncture stimulation.展开更多
Most migraine patients suffer from cutaneous allodynia; however, the underlying mechanisms are unclear. Calcitonin gene-related peptide（CGRP） plays an important role in the pathophysiology of migraine, and it is the...Most migraine patients suffer from cutaneous allodynia; however, the underlying mechanisms are unclear. Calcitonin gene-related peptide（CGRP） plays an important role in the pathophysiology of migraine, and it is therefore, a potential therapeutic target for treating the pain. In the present study, a rat model of conscious migraine, induced by repeated electrical stimulation of the superior sagittal sinus, was established and treated with electroacupuncture at Fengchi（GB20）（depth of 2–3 mm, frequency of 2/15 Hz, intensity of 0.5–1.0 m A, 15 minutes/day, for 7 consecutive days）. Electroacupuncture at GB20 significantly alleviated the decrease in hind paw and facial withdrawal thresholds and significantly lessened the increase in the levels of CGRP in the trigeminal ganglion, trigeminal nucleus caudalis and ventroposterior medial thalamic nucleus in rats with migraine. No CGRP-positive cells were detected in the trigeminal nucleus caudalis or ventroposterior medial thalamic nucleus by immunofluorescence. Our findings suggest that electroacupuncture treatment ameliorates migraine pain and associated cutaneous allodynia by modulating the trigeminovascular system ascending pathway, at least in part by inhibiting CGRP expression in the trigeminal ganglion.展开更多
OBJECTIVE:To study the effect of acupuncture at Fengchi(GB 20) on the activation of myosin light chain kinase(MLCK) in the middle meningeal artery of migraine modeled rats.METHODS:Forty-four clean grade healthy female...OBJECTIVE:To study the effect of acupuncture at Fengchi(GB 20) on the activation of myosin light chain kinase(MLCK) in the middle meningeal artery of migraine modeled rats.METHODS:Forty-four clean grade healthy female Sprague-Dawley(SD) rats were randomly divided into four groups:the control group,blank control group,Fengchi(GB 20) acupuncture group,and Fengchi(GB 20) prevention group.Neurogenic inflammation of these rats was induced by electrical stimulation.The γ-^(32)P infiltration method was then used to detect MLCK activation in the middle meningeal artery,and immunocytochemistry was applied to detect the structural protein expression of MLCK.RESULTS:The migraine model was successfully established in the rats.Compared with the control group,MLCK activation was significantly decreased in the blank control group(P < 0.01).CONCLUSION:The activation of MLCK in the middle meningeal artery was increased by acupuncture at Fengchi(GB 20),indicating its effectiveness in preventing and curing on acute migraine attacks.展开更多
The article introduces Prof.Weijia’s experience in using Fengchi(GB 20)totreat gastric ulcer,bronchial asthma,sciatica,and alopecia,etc.He considers that the point has seven general functions of dispelling pathogenic...The article introduces Prof.Weijia’s experience in using Fengchi(GB 20)totreat gastric ulcer,bronchial asthma,sciatica,and alopecia,etc.He considers that the point has seven general functions of dispelling pathogenic wind,purging pathogenic fire,relieving the depressedliver-Qi,resolving the phlegm,activating the blood,tranquilizing the mind,and checking spasm andconvulsion.It also introduces some common diseases treated with needling Fengchi and adjunctpoints,manipulations,and precautions,etc.It Is a comparatively comprehensive summary of Prof.Wei Jia’s individual experience in wonderfully using Fengchi.展开更多
This article introduces the writers’ experience in treating 100 cases of headache bymainly needling Fengchi (GB 20) and Taichong (LR 3). It is considered in TCM that headache ismostly caused by pathogenic wind, and t...This article introduces the writers’ experience in treating 100 cases of headache bymainly needling Fengchi (GB 20) and Taichong (LR 3). It is considered in TCM that headache ismostly caused by pathogenic wind, and there is a saying of "no wind, no headache". The combinationof the two points can expel both endogenous and exogenous wind. So we treat headache mainly withneedling Fengchi and Taichong. The result is good, especially for cluster headache and psychicheadache.’展开更多
The article introduces the writers’ experience in using Fengchi(GB 20) to treat sciatica,gastric ulcer and bronchial asthma, etc. The writers consider that the point has seven general functions of dispelling pathogen...The article introduces the writers’ experience in using Fengchi(GB 20) to treat sciatica,gastric ulcer and bronchial asthma, etc. The writers consider that the point has seven general functions of dispelling pathogenic wind, purging pathogenic fire, relieving the depressed liver-qi, resolvingthe phlegm, activating the blood, tranquilizing the mind, and checking spasm and c0nvulsion. It alsointroduces the manipulations and precautions of needling Fengchi.展开更多
Objective：To observe the effect of acupuncture of Fengchi （风池 GB 20） on blood pressure, serum IL-6 and plasma endothelin （ET） contents in patients with hypertension. Methods： Thirty cases of hy-pertension pati...Objective：To observe the effect of acupuncture of Fengchi （风池 GB 20） on blood pressure, serum IL-6 and plasma endothelin （ET） contents in patients with hypertension. Methods： Thirty cases of hy-pertension patients were made up of treatment group and treated with acupuncture of Fengchi （GB 20） ; while 20 healthy subjects were made up of control group. Blood pressure was determined before and after the treatment. Fasting blood samples were taken from the cubital venous vessel for detecting serum interleukin （IL）-6 and plasma ET contents with radioimmunoassay before and after acupuncture treatment. Results： Before the treatment, in hypertension patients, serum IL-6 and plasma ET levels were significant higher thanthose in healthy subjects （P〈 0.01 ）; while after the treatment, blood pressure of treatment group declined significantly （ P 〈 0.01 ）, serum IL-6 and plasma ET levels decreased considerably （ P 〈 0.01 ）. Conclusion： Acupuncture of Fengchi （GB 20） can lower blood pressure, regulate cellular immunity and secretion functions of the vascular endothelial cells in hypertension patients.展开更多
Objective: To explore therapeutic method for occipital neuralgia. Methods: A total of 90 occipital neuralgia patients were randomly divided into treatment group (n=60 cases) and control group (n=30). In treatment grou...Objective: To explore therapeutic method for occipital neuralgia. Methods: A total of 90 occipital neuralgia patients were randomly divided into treatment group (n=60 cases) and control group (n=30). In treatment group, Dazhui (GV 14) was punctured and stimulated with reinforcing method, and Fengchi (GB 20) punctured and stimulated with reducing needling method. In control group, the tender point was punctured and stimulated with reducing needling method. The acupuncture needles were retained for 30 min and the treatment was conducted once daily, with 10 times being a therapeutic course. Results: After one session of treatment, the markedly effective rates of treatment and control groups were 98% and 57% respectively. Comparison between two groups showed a considerable difference in the therapeutic effect (P<0.05). Conclusion: Acupuncture of Dazhui (GV 14) and Fengchi (GB 20) are effective in the treatment of occipital neuralgia.展开更多
Objective To explore the mechanism of electroacupuncture(EA)at"Fengchi"(GB20)and“Sishencong”(EX-HN 1)on learning and memory impairment in vascular dementia(VD)rats by observing the influences on the N-meth...Objective To explore the mechanism of electroacupuncture(EA)at"Fengchi"(GB20)and“Sishencong”(EX-HN 1)on learning and memory impairment in vascular dementia(VD)rats by observing the influences on the N-methyl-D-aspartate receptor(NMDAR)/cyclic adenosine monophosphate response element-binding protein(CREB)/brain-derived neurotrophic factor(BDNF)signaling pathway and the excitotoxicity induced by hippocampal calcium overload.Methods Thirty-two male SD rats of SPF grade were selected and randomized into a normal group(6 rats),a shamoperation group(6 rats)and an operation group(20 rats).VD model was established with the modified Pulsinelli's four-vessel occlusion(4-VO)method.Twelve rats after successfully modeled were assigned randomly into a model group and an EA group,6 rats in each one.In the EA group,EA was delivered at bilateral"Fengchi"(GB 20)and"Sishencong"(EX-HN 1),with the continuous wave,the frequency of 2 Hz and the electric current of 1 mA.Stimulation intensity was adjusted depending on the slightly trembling of rat head.EA was given once daily,30 min each time;and EA intervention was delivered for 21 days continuously.Using Morris water maze test,the learning and memory functionwas assessed.The neuronal morphology in the hippocampal CAl was observed with HE staining;the level of glutamate(GLU)in serum and hippocampal tissue,as well as the activity of calcium pump(Ca^(2+)-ATP)in the hippocampus were detected using colorimetric method.Theprotein expression ofNMDAR,calmodulindependent protein kinase II(CaMK I),phosphorylated calmodulin-dependent protein kinase I(p-CaMK II),phosphorylated cyclic phosphoradenosine effector element binding proteins(p-CREB),CREB,,and BDNF in the hippocampal CA1 was detected using immunohistochemistry.The protein expression of NMDAR,CREB,p-CREB and BDNF in the hippocampal tissue was detected using Western blot method.Results Compared to the shamoperation group,in the model group,the escape latency was prolonged and the platform crossing times of rats were reduced(P<0.01),the hippocampal neuron structure was damaged to different degrees,the structure in hippocampal CA1 was loosened,the arrangement disorganized,with clear grid-like structure;the neuronal morphology was irregular,pyknosis and even dissolution occurred,glial cells increased,blood capillarywas dilated and the inflammatory cells were infiltrated and scattered.Thelevel of GLUintheserum and hippocampal tissue and the protein expression of hippocampal NMDAR were elevated(P<0.01),the activity of Ca^(2+)-ATP and the protein expression of CaMK I,p-CaMK I,CREB,p-CREB and BDNFwere reduced(P<0.01,P<0.05);and the ratio of p-CaMK II/CaMK II and that of p-CREB/CREB were dropped(P<0.05).In comparison with the model group,in the EA group,the escape latency was shortened and the platform crossing times of rats rose(P<0.01),the arrangement was improved in the hippocampal CA1,the neuronal morphology was intact,the nucleoli were relatively clear and the pyknosis or dissolution were attenuated,the numbers of glial cells reduced relatively,the dilation of blood capillary was alleviated.The level of GLU in the serum and hippocampal tissue and the protein expression of NMDAR were reduced in the hippocampal tissue(P<0.01),the activity of Ca^(2+)-ATP and the protein expression of CaMK II,p-CaMK I,CREB,p-CREB and BDNF were elevated(P<0.05,P<0.01);the ratio of p-CaMK II/CaMK II and that of p-CREB/CREB increased(P<0.05).Conclusion EA at"Fengchi"(GB 20)and"Sishencong"(EX-HN 1)can attenuate learning and memory impairment in VD rats,which may be obtained by reducing GLU level in hippocampal tissue,inhibiting hippocampal excitotoxicity,mediating protein expression related to the NMDAR/CREB/BDNFsignalingpathway,and maintaining neuronal survival and growth.展开更多
Objective:To investigate the potential mechanism of electroacupuncture(EA)at bilateral Fengchi(GB20)in treating cerebral ischemia-reperfusion injury and to provide a scientific basis for future experimental research a...Objective:To investigate the potential mechanism of electroacupuncture(EA)at bilateral Fengchi(GB20)in treating cerebral ischemia-reperfusion injury and to provide a scientific basis for future experimental research and clinical applications.Methods:Forty male specific-pathogen-free Sprague-Dawley rats were randomly divided into four groups:a normal group,a normal with EA group,a model group,and a model with EA group,with 10 rats in each group.The normal group received no intervention.The normal with EA group received EA at bilateral Fengchi(GB20).The model group underwent middle cerebral artery occlusion(MCAO)using the suture.The model with EA group underwent MCAO and received EA at bilateral Fengchi(GB20).Cerebral blood flow was monitored using a laser Doppler cerebral blood flow meter.Neurologic damage was assessed using the neurologic deficit score,and motor ability was observed using the CatWalk gait system.The expression of glial fibrillary acidic protein(GFAP)and neuronal nuclei(NeuN)protein,the neuron markers,was detected by Western blotting.The protein expression levels of GFAP and NeuN,as well as the number of positive cells in the motor cortex,were detected using immunofluorescence.Results:Compared to the normal group,the cerebral blood flow values in the model group and the model with EA group decreased by more than 50%during the modeling process(P<0.01)and returned to pre-modeling levels after reperfusion(P>0.05).The neurologic deficit score increased(P<0.05),the average motor velocity decreased(P<0.05),GFAP protein expression and the number of positive cells in the motor cortex increased(P<0.05),and the NeuN protein expression and the number of positive cells decreased(P<0.05)in the model group.Compared to the model group,the neurologic deficit score decreased(P<0.05),the average motor velocity accelerated(P<0.05),GFAP and NeuN protein expression and the number of positive cells in the motor cortex increased(P<0.01)in the model with EA group.Conclusion:EA at bilateral Fengchi(GB20)can reduce neuronal loss and increase GFAP and NeuN protein expression in the motor cortex of rats after ischemia-reperfusion,improve the motor function after ischemic stroke,and accelerate the recovery of balance and stability of the affected limbs.展开更多
基金the Study on the Mechanism of Action of Dutong and Tiaoshen Acupuncture in Improving Upper Limb Motor Function After Ischemic Stroke based on Functional Near-infrared Spectroscopy Technology(Science and Technology Program of Panyu,2022-Z04-107)。
文摘OBJECTIVE:To take Baihui(GV20)and Fengchi(GB20)targeting inflammatory response to regulate migraine as an example to describe a new method for studying the mechanism of stimulating acupoints.METHODS:The target information of Baihui(GV20)and Fengchi(GB20)was retrieved,and after intersection with migraine,Kyoto Encyclopedia of Genes and Genomes(KEGG),Reactome,and Uni Prot Keywords were used for functional enrichment.After selecting the main pathway,rats were selected and nitroglycerin was used for modeling,and the behavioral scores,inflammatory factors,heme oxygenase 1(HMOX1),protein kinase B(AKT1),signal transducer and activator of transcription 3(STAT3),phosphorylated extracellular signal-regulated kinase 1/2(P-ERK1/ERK2)and other states of the rats in the acupuncture,twisting,and electroacupuncture groups were compared.RESULTS:A total of 135 Baihui(GV20)targets and 27 Fengchi(GB20)targets were collected.A total of 73 target information were obtained after the intersection of these targets in migraine.These 73 targets have three main pathways:hypoxia-inducible factor 1(HIF-1)signaling pathway,signaling by interleukins and inflammatory response.The main targets in the pathway were verified and found that interleukin-1 beta(IL-1β),interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α)and HMOX1,AKT1,STAT3,P-ERK1/ERK2 can be regulated by Baihui(GV20)and Fengchi(GB20).CONCLUSION:Baihui(GV20)and Fengchi(GB20)can regulate migraine by regulating inflammatory factors and HMOX1,AKT1,STAT3,P-ERK1/ERK2 and other changes in HIF-1 signaling pathway,Signaling by Interleukins and Inflammatory response pathways.Based on systems biology and network pharmacology,and with the model of"acupoint-target-disease",explore the research methods of systematic acupuncture and moxibustion.We believe this is a usable research direction for exploring the mechanism of acupuncture stimulation.
基金supported by the National Natural Science Foundation of China,No.81603683a grant from the National Basic Research Program of China,No.2014CB543203a grant from the Beijing Municipal Science&Technology Commission of China,No.Z171100001017033
文摘Most migraine patients suffer from cutaneous allodynia; however, the underlying mechanisms are unclear. Calcitonin gene-related peptide（CGRP） plays an important role in the pathophysiology of migraine, and it is therefore, a potential therapeutic target for treating the pain. In the present study, a rat model of conscious migraine, induced by repeated electrical stimulation of the superior sagittal sinus, was established and treated with electroacupuncture at Fengchi（GB20）（depth of 2–3 mm, frequency of 2/15 Hz, intensity of 0.5–1.0 m A, 15 minutes/day, for 7 consecutive days）. Electroacupuncture at GB20 significantly alleviated the decrease in hind paw and facial withdrawal thresholds and significantly lessened the increase in the levels of CGRP in the trigeminal ganglion, trigeminal nucleus caudalis and ventroposterior medial thalamic nucleus in rats with migraine. No CGRP-positive cells were detected in the trigeminal nucleus caudalis or ventroposterior medial thalamic nucleus by immunofluorescence. Our findings suggest that electroacupuncture treatment ameliorates migraine pain and associated cutaneous allodynia by modulating the trigeminovascular system ascending pathway, at least in part by inhibiting CGRP expression in the trigeminal ganglion.
基金the Beijing Natural Science Found Program[Fengchi point(GB 20)Controlling Mechanisn of MLCK Signal System of the Migraine,No.7092054
文摘OBJECTIVE:To study the effect of acupuncture at Fengchi(GB 20) on the activation of myosin light chain kinase(MLCK) in the middle meningeal artery of migraine modeled rats.METHODS:Forty-four clean grade healthy female Sprague-Dawley(SD) rats were randomly divided into four groups:the control group,blank control group,Fengchi(GB 20) acupuncture group,and Fengchi(GB 20) prevention group.Neurogenic inflammation of these rats was induced by electrical stimulation.The γ-^(32)P infiltration method was then used to detect MLCK activation in the middle meningeal artery,and immunocytochemistry was applied to detect the structural protein expression of MLCK.RESULTS:The migraine model was successfully established in the rats.Compared with the control group,MLCK activation was significantly decreased in the blank control group(P < 0.01).CONCLUSION:The activation of MLCK in the middle meningeal artery was increased by acupuncture at Fengchi(GB 20),indicating its effectiveness in preventing and curing on acute migraine attacks.
文摘The article introduces Prof.Weijia’s experience in using Fengchi(GB 20)totreat gastric ulcer,bronchial asthma,sciatica,and alopecia,etc.He considers that the point has seven general functions of dispelling pathogenic wind,purging pathogenic fire,relieving the depressedliver-Qi,resolving the phlegm,activating the blood,tranquilizing the mind,and checking spasm andconvulsion.It also introduces some common diseases treated with needling Fengchi and adjunctpoints,manipulations,and precautions,etc.It Is a comparatively comprehensive summary of Prof.Wei Jia’s individual experience in wonderfully using Fengchi.
文摘This article introduces the writers’ experience in treating 100 cases of headache bymainly needling Fengchi (GB 20) and Taichong (LR 3). It is considered in TCM that headache ismostly caused by pathogenic wind, and there is a saying of "no wind, no headache". The combinationof the two points can expel both endogenous and exogenous wind. So we treat headache mainly withneedling Fengchi and Taichong. The result is good, especially for cluster headache and psychicheadache.’
文摘The article introduces the writers’ experience in using Fengchi(GB 20) to treat sciatica,gastric ulcer and bronchial asthma, etc. The writers consider that the point has seven general functions of dispelling pathogenic wind, purging pathogenic fire, relieving the depressed liver-qi, resolvingthe phlegm, activating the blood, tranquilizing the mind, and checking spasm and c0nvulsion. It alsointroduces the manipulations and precautions of needling Fengchi.
文摘Objective：To observe the effect of acupuncture of Fengchi （风池 GB 20） on blood pressure, serum IL-6 and plasma endothelin （ET） contents in patients with hypertension. Methods： Thirty cases of hy-pertension patients were made up of treatment group and treated with acupuncture of Fengchi （GB 20） ; while 20 healthy subjects were made up of control group. Blood pressure was determined before and after the treatment. Fasting blood samples were taken from the cubital venous vessel for detecting serum interleukin （IL）-6 and plasma ET contents with radioimmunoassay before and after acupuncture treatment. Results： Before the treatment, in hypertension patients, serum IL-6 and plasma ET levels were significant higher thanthose in healthy subjects （P〈 0.01 ）; while after the treatment, blood pressure of treatment group declined significantly （ P 〈 0.01 ）, serum IL-6 and plasma ET levels decreased considerably （ P 〈 0.01 ）. Conclusion： Acupuncture of Fengchi （GB 20） can lower blood pressure, regulate cellular immunity and secretion functions of the vascular endothelial cells in hypertension patients.
文摘Objective: To explore therapeutic method for occipital neuralgia. Methods: A total of 90 occipital neuralgia patients were randomly divided into treatment group (n=60 cases) and control group (n=30). In treatment group, Dazhui (GV 14) was punctured and stimulated with reinforcing method, and Fengchi (GB 20) punctured and stimulated with reducing needling method. In control group, the tender point was punctured and stimulated with reducing needling method. The acupuncture needles were retained for 30 min and the treatment was conducted once daily, with 10 times being a therapeutic course. Results: After one session of treatment, the markedly effective rates of treatment and control groups were 98% and 57% respectively. Comparison between two groups showed a considerable difference in the therapeutic effect (P<0.05). Conclusion: Acupuncture of Dazhui (GV 14) and Fengchi (GB 20) are effective in the treatment of occipital neuralgia.
文摘Objective To explore the mechanism of electroacupuncture(EA)at"Fengchi"(GB20)and“Sishencong”(EX-HN 1)on learning and memory impairment in vascular dementia(VD)rats by observing the influences on the N-methyl-D-aspartate receptor(NMDAR)/cyclic adenosine monophosphate response element-binding protein(CREB)/brain-derived neurotrophic factor(BDNF)signaling pathway and the excitotoxicity induced by hippocampal calcium overload.Methods Thirty-two male SD rats of SPF grade were selected and randomized into a normal group(6 rats),a shamoperation group(6 rats)and an operation group(20 rats).VD model was established with the modified Pulsinelli's four-vessel occlusion(4-VO)method.Twelve rats after successfully modeled were assigned randomly into a model group and an EA group,6 rats in each one.In the EA group,EA was delivered at bilateral"Fengchi"(GB 20)and"Sishencong"(EX-HN 1),with the continuous wave,the frequency of 2 Hz and the electric current of 1 mA.Stimulation intensity was adjusted depending on the slightly trembling of rat head.EA was given once daily,30 min each time;and EA intervention was delivered for 21 days continuously.Using Morris water maze test,the learning and memory functionwas assessed.The neuronal morphology in the hippocampal CAl was observed with HE staining;the level of glutamate(GLU)in serum and hippocampal tissue,as well as the activity of calcium pump(Ca^(2+)-ATP)in the hippocampus were detected using colorimetric method.Theprotein expression ofNMDAR,calmodulindependent protein kinase II(CaMK I),phosphorylated calmodulin-dependent protein kinase I(p-CaMK II),phosphorylated cyclic phosphoradenosine effector element binding proteins(p-CREB),CREB,,and BDNF in the hippocampal CA1 was detected using immunohistochemistry.The protein expression of NMDAR,CREB,p-CREB and BDNF in the hippocampal tissue was detected using Western blot method.Results Compared to the shamoperation group,in the model group,the escape latency was prolonged and the platform crossing times of rats were reduced(P<0.01),the hippocampal neuron structure was damaged to different degrees,the structure in hippocampal CA1 was loosened,the arrangement disorganized,with clear grid-like structure;the neuronal morphology was irregular,pyknosis and even dissolution occurred,glial cells increased,blood capillarywas dilated and the inflammatory cells were infiltrated and scattered.Thelevel of GLUintheserum and hippocampal tissue and the protein expression of hippocampal NMDAR were elevated(P<0.01),the activity of Ca^(2+)-ATP and the protein expression of CaMK I,p-CaMK I,CREB,p-CREB and BDNFwere reduced(P<0.01,P<0.05);and the ratio of p-CaMK II/CaMK II and that of p-CREB/CREB were dropped(P<0.05).In comparison with the model group,in the EA group,the escape latency was shortened and the platform crossing times of rats rose(P<0.01),the arrangement was improved in the hippocampal CA1,the neuronal morphology was intact,the nucleoli were relatively clear and the pyknosis or dissolution were attenuated,the numbers of glial cells reduced relatively,the dilation of blood capillary was alleviated.The level of GLU in the serum and hippocampal tissue and the protein expression of NMDAR were reduced in the hippocampal tissue(P<0.01),the activity of Ca^(2+)-ATP and the protein expression of CaMK II,p-CaMK I,CREB,p-CREB and BDNF were elevated(P<0.05,P<0.01);the ratio of p-CaMK II/CaMK II and that of p-CREB/CREB increased(P<0.05).Conclusion EA at"Fengchi"(GB 20)and"Sishencong"(EX-HN 1)can attenuate learning and memory impairment in VD rats,which may be obtained by reducing GLU level in hippocampal tissue,inhibiting hippocampal excitotoxicity,mediating protein expression related to the NMDAR/CREB/BDNFsignalingpathway,and maintaining neuronal survival and growth.
文摘Objective:To investigate the potential mechanism of electroacupuncture(EA)at bilateral Fengchi(GB20)in treating cerebral ischemia-reperfusion injury and to provide a scientific basis for future experimental research and clinical applications.Methods:Forty male specific-pathogen-free Sprague-Dawley rats were randomly divided into four groups:a normal group,a normal with EA group,a model group,and a model with EA group,with 10 rats in each group.The normal group received no intervention.The normal with EA group received EA at bilateral Fengchi(GB20).The model group underwent middle cerebral artery occlusion(MCAO)using the suture.The model with EA group underwent MCAO and received EA at bilateral Fengchi(GB20).Cerebral blood flow was monitored using a laser Doppler cerebral blood flow meter.Neurologic damage was assessed using the neurologic deficit score,and motor ability was observed using the CatWalk gait system.The expression of glial fibrillary acidic protein(GFAP)and neuronal nuclei(NeuN)protein,the neuron markers,was detected by Western blotting.The protein expression levels of GFAP and NeuN,as well as the number of positive cells in the motor cortex,were detected using immunofluorescence.Results:Compared to the normal group,the cerebral blood flow values in the model group and the model with EA group decreased by more than 50%during the modeling process(P<0.01)and returned to pre-modeling levels after reperfusion(P>0.05).The neurologic deficit score increased(P<0.05),the average motor velocity decreased(P<0.05),GFAP protein expression and the number of positive cells in the motor cortex increased(P<0.05),and the NeuN protein expression and the number of positive cells decreased(P<0.05)in the model group.Compared to the model group,the neurologic deficit score decreased(P<0.05),the average motor velocity accelerated(P<0.05),GFAP and NeuN protein expression and the number of positive cells in the motor cortex increased(P<0.01)in the model with EA group.Conclusion:EA at bilateral Fengchi(GB20)can reduce neuronal loss and increase GFAP and NeuN protein expression in the motor cortex of rats after ischemia-reperfusion,improve the motor function after ischemic stroke,and accelerate the recovery of balance and stability of the affected limbs.
