Although the effects of diet on intestinal microbiotas have been well investigated,the effect of feeding time on the rhythm of gut microbiota remains uncertain.A 9-week feeding trial on Pelteobagrus fulvidraco was con...Although the effects of diet on intestinal microbiotas have been well investigated,the effect of feeding time on the rhythm of gut microbiota remains uncertain.A 9-week feeding trial on Pelteobagrus fulvidraco was conducted.Two triplicate groups of fish were fed identically either at 8:00(day)or at 20:00(night).After 9 weeks,three fish from each cage were sacrificed every 4 h for 24 h.The intestinal contents were analyzed using high-throughput sequencing.Three phyla(Fusobacteria,Proteobacteria,and Verrucomicrobia),and three genera(Cetobacterium,Akkermansia,and Clostridium)were dominant at every time point.Moreover,33.02%of detected amplicon sequence variants showed cyclical variations in fish fed at 20:00,and 3.47%in fish fed at 8:00.Among the top 20 genera,17 showed cyclical variations in the 20:00 group,and only two in the 8:00 group.Rhodobacter,Ralstonia,Mycobacterium,Lactococcus,Halomonas,Akkermansia,and Acinetobacter contributed more to the predicted functional pathways.Moreover,144 and 38 pathways exhibited daily cyclical fluctuations in fish fed at 20:00 and 8:00,respectively.Overall,certain intestinal microorganisms showed a circadian rhythm in P.fulvidraco,and this circadian rhythm was affected by shifted feeding times.展开更多
The present study was to investigate the effects of feeding time on growth of the Chinese soft-shelled turtle. The experiment was carried out with a photoperiod of 12L （light）： 12D （dark） with lights on at 06：00...The present study was to investigate the effects of feeding time on growth of the Chinese soft-shelled turtle. The experiment was carried out with a photoperiod of 12L （light）： 12D （dark） with lights on at 06：00 and off at 18：00. Turtles were maintained at a temperature of 32 - 0.2 ℃ in tanks throughout the length of the experiment. The turtles in group 1 to group 6 were fed respectively at 00：00, 04：00, 08：00, 12：00, 16：00 and 20：00 with 60 turtles each （Initial body weight 88.27 - 0.09 g）. Acrophases of postprandial RNA/DNA ratio in liver in each group was shown between 5h and 7h after feeding. A positive linear correlation could be seen between specific growth rate （SGR） and RNA/ DNA：SGR=t.1586RNA/DNA-0.7097 （r = 0.9328, P = 0.0066）. The results indicated that the values at the acrophases of about 6h after feeding might be used as an instantaneous growth index in the Chinese soft-shelled turtle. The turtles in group 1 grew better than group 3, group 4, group 5, group 6, because they ate more, but they ate more and grew slower than group 2, whose feed conversion rate was also higher. Meanwhile, the SGR and feeding rate of turtles fed at 12：00 were the lowest from the six groups （P 〈 0.05）. Turtles fed in group 1, group 2 and group 6 developed more heavy final body weight （FBW）, higher feeding rate and SGR than the other three groups. This probably suggested that turtles fed in scotophase grew better than that fed at photophase in total.展开更多
Dear Editor,Obesity is a pandemic, chronic, multifactorial disease that contributes to numerous conditions, including metabolic dysfunction-associated fatty liver disease (MAFLD) [1]. MAFLD is characterized by excessi...Dear Editor,Obesity is a pandemic, chronic, multifactorial disease that contributes to numerous conditions, including metabolic dysfunction-associated fatty liver disease (MAFLD) [1]. MAFLD is characterized by excessive triglyceride accumulation in the liver in the presence of at least one cardiometabolic risk factor (e.g. obesity, elevated blood pressure, insulin resistance, dyslipidemia, or elevated plasma glucose) and the absence of other apparent causes. Obese individuals may progress to the more severe condition of metabolic dysfunction-associated steatohepatitis (MASH), an advanced stage of MAFLD characterized by hepatic fat accumulation and inflammation. MAFLD represents the initial phase of liver disease, with MASH as the intermediate stage and fibrosis or cirrhosis as the final stage if left untreated [1].展开更多
Objective of this study was to evaluate the ingestive behavior,feed efficiency,and nutritional and physiological parameters of sheep that were fed diets based on byproducts from the processing of cashew.The experiment...Objective of this study was to evaluate the ingestive behavior,feed efficiency,and nutritional and physiological parameters of sheep that were fed diets based on byproducts from the processing of cashew.The experiment was conducted in a completely randomized design with a 4×2 factorial arrangement with four levels of inclusion(6%,11%,16%,and 21%of cashew byproduct)and two forms of processing—with chemical treatment(CT)and without chemical treatment(NCT).The interaction levels of inclusion of the byproduct of cashew versus chemical treatments was not(P>0.05)for the dry matter intake,consumption of organic matter.No effect was observed(P>0.05)for the intake of dry matter in function of the type of chemical treatment used in the byproduct of cashew.There was no effect of interaction(P<0.05)between levels of inclusion and chemical treatment applied or not on the byproduct of cashew for the coefficients of digestibility of dry matter,organic matter and crude protein(P>0.05).The inclusion of the byproduct of cashew did not influence the behavioral parameters,intake and digestibility of the diets of sheep,being recommended to use up to the level of 21%.展开更多
Understanding how individuals manage costs during the migration period is challenging because individuals are difficult to follow between sites; the advent of hybrid Global Positioning Systemacceleration （ACC） track...Understanding how individuals manage costs during the migration period is challenging because individuals are difficult to follow between sites; the advent of hybrid Global Positioning Systemacceleration （ACC） tracking devices enables researchers to link spatial and temporal attributes of avian migration with behavior for the first time ever. We fitted these devices on male Greenland white-fronted geese Anser albifrons flavirostris wintering at 2 sites （Loch Ken, Scotland and Wexford, Ireland） to understand whether birds migrating further during spring fed more on winter- ing and staging areas in advance of migration episodes. Although Irish birds flew significantly further （ca. 300 kin） than Scottish birds during spring, their cumulative hours of migratory flight, flight speed during migration, and overall dynamic body ACC （i.e., a proxy for energy expenditure） were not significantly different. Further, Irish birds did not feed significantly more or expend significantly more energy in advance of migration episodes. These results suggest broad individual plasticity in this species, although Scottish birds arriving on breeding areas in Greenland with greater energy stores （because they migrated less） may be better prepared for food scarcity, which might increase their reproductive success.展开更多
Background The aim of this study was to compare nasogastric(NG)feeding with nasojejunal(NJ)feeding when treating pediatric patients with acute pancreatitis(AP)..Methods We performed a single-center,prospective,randomi...Background The aim of this study was to compare nasogastric(NG)feeding with nasojejunal(NJ)feeding when treating pediatric patients with acute pancreatitis(AP)..Methods We performed a single-center,prospective,randomized,active-controlled trial involving 77 pediatric patients with AP from April 2014 to December 2017.The patients were randomized into two groups:the NG tube feeding group(34 patients)and the NJ tube feeding group(33 patients).The primary outcome measures included the enteral nutrition intoler-ance,the length of tube feeding time,the recurrent pain of pancreatitis and complications.Results A total of 62 patients with AP(31 patients for each group)came into the final analysis.No differences were found in baseline characteristics,pediatric AP score and computed tomography severity score between the lwo groups.Three(9.7%)patients in the NG group and one(3.2%)patient in the NJ group developed intolerance(relative risk=3.00,95%confidence interval 0.33-27.29,P=0.612).The tube feeding time and length of hospital stay of the NG group were significantly shorter than those of the NJ group(P=0.016 and 0.027,respectively).No patient died in the trial.No significant differences were found in recurrent pain,complications,nutrition delivery efficacy,and side effects between the two groups.Conclusions NG tube feeding appears to be effective and safe for acute pediatric pancreatitis compared with NJ tube feeding.In addition,high qualifed,large sample sized,randomized controlled trials in pediatric population are needed.展开更多
Alzheimer’s disease(AD)is a progressive neurodegenerative disorder characterized by cognitive decline and pathological brain changes.While aging is the primary risk factor,circadian rhythm disruption(CRD)is increasin...Alzheimer’s disease(AD)is a progressive neurodegenerative disorder characterized by cognitive decline and pathological brain changes.While aging is the primary risk factor,circadian rhythm disruption(CRD)is increasingly recognized as a central driver of AD pathology.CRD exacerbates oxidative stress,systemic inflammation,and gut microbiome dysbiosis,impairing sleep-wake cycles,disrupting metabolic homeostasis,and promoting neuroinflammation,ultimately accelerating disease progression.Oxidative stress,a key factor in neuronal damage,is both a cause and consequence of circadian misalignment,while mitochondrial dysfunction further amplifies oxidative damage,impairing synaptic function and cognitive stability.Additionally,gut microbiome dysbiosis contributes to neuroinflammatory processes,worsening neurodegeneration.Given these complex interactions,this review aims to elucidate the role of CRD in AD pathology and explore potential therapeutic interventions targeting circadian dysfunction.Specifically,it examines the efficacy of time-restricted feeding(TRF),a dietary strategy that aligns food intake with circadian rhythms.TRF has shown promise in restoring circadian function,reducing oxidative stress,improving mitochondrial health,and promoting gut microbiome diversity.By addressing CRD,TRF may offer a novel approach to mitigating AD pathologies.This review also identifies current research gaps and future directions for developing circadian-based interventions in AD prevention and treatment.展开更多
Climate change is the first real challenge of this global generation,and,so far,it hasn’t gone that well.Whether it’s rising sea levels threatening cities like Shanghai or expanding vectors of malaria,China must cha...Climate change is the first real challenge of this global generation,and,so far,it hasn’t gone that well.Whether it’s rising sea levels threatening cities like Shanghai or expanding vectors of malaria,China must change with the climate.As the glaciers of China’s Third Pole retreat,as new and more regular floods attack first-tier cities and rural towns alike,as wheat yields decrease in the north and entire lakes dry up in the west,the days of ignoring climate change have展开更多
基金Supported by the Sichuan Science and Technology Program(Nos.2021YFYZ0015,2023NSFSC0241)。
文摘Although the effects of diet on intestinal microbiotas have been well investigated,the effect of feeding time on the rhythm of gut microbiota remains uncertain.A 9-week feeding trial on Pelteobagrus fulvidraco was conducted.Two triplicate groups of fish were fed identically either at 8:00(day)or at 20:00(night).After 9 weeks,three fish from each cage were sacrificed every 4 h for 24 h.The intestinal contents were analyzed using high-throughput sequencing.Three phyla(Fusobacteria,Proteobacteria,and Verrucomicrobia),and three genera(Cetobacterium,Akkermansia,and Clostridium)were dominant at every time point.Moreover,33.02%of detected amplicon sequence variants showed cyclical variations in fish fed at 20:00,and 3.47%in fish fed at 8:00.Among the top 20 genera,17 showed cyclical variations in the 20:00 group,and only two in the 8:00 group.Rhodobacter,Ralstonia,Mycobacterium,Lactococcus,Halomonas,Akkermansia,and Acinetobacter contributed more to the predicted functional pathways.Moreover,144 and 38 pathways exhibited daily cyclical fluctuations in fish fed at 20:00 and 8:00,respectively.Overall,certain intestinal microorganisms showed a circadian rhythm in P.fulvidraco,and this circadian rhythm was affected by shifted feeding times.
基金supported by the National Natural Science Foundation of China (No.31172085)
文摘The present study was to investigate the effects of feeding time on growth of the Chinese soft-shelled turtle. The experiment was carried out with a photoperiod of 12L （light）： 12D （dark） with lights on at 06：00 and off at 18：00. Turtles were maintained at a temperature of 32 - 0.2 ℃ in tanks throughout the length of the experiment. The turtles in group 1 to group 6 were fed respectively at 00：00, 04：00, 08：00, 12：00, 16：00 and 20：00 with 60 turtles each （Initial body weight 88.27 - 0.09 g）. Acrophases of postprandial RNA/DNA ratio in liver in each group was shown between 5h and 7h after feeding. A positive linear correlation could be seen between specific growth rate （SGR） and RNA/ DNA：SGR=t.1586RNA/DNA-0.7097 （r = 0.9328, P = 0.0066）. The results indicated that the values at the acrophases of about 6h after feeding might be used as an instantaneous growth index in the Chinese soft-shelled turtle. The turtles in group 1 grew better than group 3, group 4, group 5, group 6, because they ate more, but they ate more and grew slower than group 2, whose feed conversion rate was also higher. Meanwhile, the SGR and feeding rate of turtles fed at 12：00 were the lowest from the six groups （P 〈 0.05）. Turtles fed in group 1, group 2 and group 6 developed more heavy final body weight （FBW）, higher feeding rate and SGR than the other three groups. This probably suggested that turtles fed in scotophase grew better than that fed at photophase in total.
基金supported by the National Council for Scientific and Technological Development (CNPq,process numbers 311939/2020-1, 441725/2023-6, and 308999/2022-3)the Coordination for the Improvement of Higher Education Personnel (CAPES,finance code 001)the S?o Paulo Research Foundation (FAPESP,process numbers 2019/13210-0, 2019/11820-5, 2020/13443-1, 2022/14758-1, 2024/16630-8, and 2022/08930-6)
文摘Dear Editor,Obesity is a pandemic, chronic, multifactorial disease that contributes to numerous conditions, including metabolic dysfunction-associated fatty liver disease (MAFLD) [1]. MAFLD is characterized by excessive triglyceride accumulation in the liver in the presence of at least one cardiometabolic risk factor (e.g. obesity, elevated blood pressure, insulin resistance, dyslipidemia, or elevated plasma glucose) and the absence of other apparent causes. Obese individuals may progress to the more severe condition of metabolic dysfunction-associated steatohepatitis (MASH), an advanced stage of MAFLD characterized by hepatic fat accumulation and inflammation. MAFLD represents the initial phase of liver disease, with MASH as the intermediate stage and fibrosis or cirrhosis as the final stage if left untreated [1].
文摘Objective of this study was to evaluate the ingestive behavior,feed efficiency,and nutritional and physiological parameters of sheep that were fed diets based on byproducts from the processing of cashew.The experiment was conducted in a completely randomized design with a 4×2 factorial arrangement with four levels of inclusion(6%,11%,16%,and 21%of cashew byproduct)and two forms of processing—with chemical treatment(CT)and without chemical treatment(NCT).The interaction levels of inclusion of the byproduct of cashew versus chemical treatments was not(P>0.05)for the dry matter intake,consumption of organic matter.No effect was observed(P>0.05)for the intake of dry matter in function of the type of chemical treatment used in the byproduct of cashew.There was no effect of interaction(P<0.05)between levels of inclusion and chemical treatment applied or not on the byproduct of cashew for the coefficients of digestibility of dry matter,organic matter and crude protein(P>0.05).The inclusion of the byproduct of cashew did not influence the behavioral parameters,intake and digestibility of the diets of sheep,being recommended to use up to the level of 21%.
文摘Understanding how individuals manage costs during the migration period is challenging because individuals are difficult to follow between sites; the advent of hybrid Global Positioning Systemacceleration （ACC） tracking devices enables researchers to link spatial and temporal attributes of avian migration with behavior for the first time ever. We fitted these devices on male Greenland white-fronted geese Anser albifrons flavirostris wintering at 2 sites （Loch Ken, Scotland and Wexford, Ireland） to understand whether birds migrating further during spring fed more on winter- ing and staging areas in advance of migration episodes. Although Irish birds flew significantly further （ca. 300 kin） than Scottish birds during spring, their cumulative hours of migratory flight, flight speed during migration, and overall dynamic body ACC （i.e., a proxy for energy expenditure） were not significantly different. Further, Irish birds did not feed significantly more or expend significantly more energy in advance of migration episodes. These results suggest broad individual plasticity in this species, although Scottish birds arriving on breeding areas in Greenland with greater energy stores （because they migrated less） may be better prepared for food scarcity, which might increase their reproductive success.
基金supported by Scientific Research Fund of Zhejiang Provincial Education Department(Y201738367).
文摘Background The aim of this study was to compare nasogastric(NG)feeding with nasojejunal(NJ)feeding when treating pediatric patients with acute pancreatitis(AP)..Methods We performed a single-center,prospective,randomized,active-controlled trial involving 77 pediatric patients with AP from April 2014 to December 2017.The patients were randomized into two groups:the NG tube feeding group(34 patients)and the NJ tube feeding group(33 patients).The primary outcome measures included the enteral nutrition intoler-ance,the length of tube feeding time,the recurrent pain of pancreatitis and complications.Results A total of 62 patients with AP(31 patients for each group)came into the final analysis.No differences were found in baseline characteristics,pediatric AP score and computed tomography severity score between the lwo groups.Three(9.7%)patients in the NG group and one(3.2%)patient in the NJ group developed intolerance(relative risk=3.00,95%confidence interval 0.33-27.29,P=0.612).The tube feeding time and length of hospital stay of the NG group were significantly shorter than those of the NJ group(P=0.016 and 0.027,respectively).No patient died in the trial.No significant differences were found in recurrent pain,complications,nutrition delivery efficacy,and side effects between the two groups.Conclusions NG tube feeding appears to be effective and safe for acute pediatric pancreatitis compared with NJ tube feeding.In addition,high qualifed,large sample sized,randomized controlled trials in pediatric population are needed.
基金funded by National Institutes of Health(NIH)grants AG065992,AG068550 and NS133378 to Girish C.Melkani(USA)supported in part by UAB Nathan Shock Center P30 AG050886(USA).
文摘Alzheimer’s disease(AD)is a progressive neurodegenerative disorder characterized by cognitive decline and pathological brain changes.While aging is the primary risk factor,circadian rhythm disruption(CRD)is increasingly recognized as a central driver of AD pathology.CRD exacerbates oxidative stress,systemic inflammation,and gut microbiome dysbiosis,impairing sleep-wake cycles,disrupting metabolic homeostasis,and promoting neuroinflammation,ultimately accelerating disease progression.Oxidative stress,a key factor in neuronal damage,is both a cause and consequence of circadian misalignment,while mitochondrial dysfunction further amplifies oxidative damage,impairing synaptic function and cognitive stability.Additionally,gut microbiome dysbiosis contributes to neuroinflammatory processes,worsening neurodegeneration.Given these complex interactions,this review aims to elucidate the role of CRD in AD pathology and explore potential therapeutic interventions targeting circadian dysfunction.Specifically,it examines the efficacy of time-restricted feeding(TRF),a dietary strategy that aligns food intake with circadian rhythms.TRF has shown promise in restoring circadian function,reducing oxidative stress,improving mitochondrial health,and promoting gut microbiome diversity.By addressing CRD,TRF may offer a novel approach to mitigating AD pathologies.This review also identifies current research gaps and future directions for developing circadian-based interventions in AD prevention and treatment.
文摘Climate change is the first real challenge of this global generation,and,so far,it hasn’t gone that well.Whether it’s rising sea levels threatening cities like Shanghai or expanding vectors of malaria,China must change with the climate.As the glaciers of China’s Third Pole retreat,as new and more regular floods attack first-tier cities and rural towns alike,as wheat yields decrease in the north and entire lakes dry up in the west,the days of ignoring climate change have
