[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of ...[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of （46.34±3.06） kg were randomly divided into three groups： con- trol group （protein level 15%, lysine level 0.83%）, experimental grnup 1 （protein level 13%, 0.83%）, and experimental group II （protein level 13%, lysine level 1.03）, each group had three replicates of four pigs. The trial lasted 35 d. [Result] The growth performance of Hainan black pig in grnup II had no significan! difference with that in contrail group （P〉0.05）. The apparent digestibility of chide protein and phosphorus of Hainan black pigs in Group II increased significantly （P〈0.05）, which were increased by 10.08% and 11.85% respectively compared to the control group; the apparent digestibility of calcium was increased, but the difference was not signifieant （P〉0.05）. [Conclusion] It will not reduce the growth performance of Hainan blaek pig when the protein level of diet is reduced by 2% and Ihe lysine level is increased to 1.03%. It is recommended that the diet can be used in the production of llainan black pig.展开更多
Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transfor...Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transformed into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of ^15N deposition of the experimental diet taken by each goat.The ^15N excreted through the feces and urine for the three goats accounted for 34.8,33.2 and 33.9% of the total amount of ^15N deposition in the feed of the 3 goats.The recovery of total ^15N for the two slaughtered goats were 73.3 and 57.5%,with the corresponding rates of ^15N loss 26.7 and 42.5% respectively.The digestibilities of total amino acids for Goats 1 and 3 were 68.7 and 54.0%,and the digestibilties of carbohydrates for the two goats were 74.8 and 67.7% respectively.展开更多
Many animal feed grains contain high β-glucan in the cell wall. Pigs do not secret β-glucanase to degrade the β-glucan in their feed. The indigestible β-glucan not only blocks the release of nutrients from the gra...Many animal feed grains contain high β-glucan in the cell wall. Pigs do not secret β-glucanase to degrade the β-glucan in their feed. The indigestible β-glucan not only blocks the release of nutrients from the grain cell wall, but also increases the digesta viscosity in the gastrointestinal tract of pigs. Therefore, dietary β-glucan significantly inhibits nutrient digestion and absorption in pigs. Transgenic expression of β-glucanase in the digestive tract of pigs may offer a solution to solve this problem. In the current study, four artificial codon-optimized β-glucanases genes was prepared and expressed in porcine cells. Only p Bg A and p Egx showed high activity in transfected pig kidney cells. To improve the p H range and p H stability of β-glucanase, the two β-glucanases, p Bg A and p Egx, were co-expressed in pig kidney cells and salivary gland cells by Linker A3 or 2A peptide. The resulting dual enzymes of p Bg A3 p Eg and p Bg2 Ap Eg showed significantly enlarged p H range and significantly increased p H stability, as compared to parental enzymes. These results provide useful data for future study on increasing the feed digestibility of pigs by transgenic expression of β-glucanase in their salivary glands.展开更多
Background:To optimize animal production systems for future sustainability,research now focuses on improving feed quality to enhance livestock performance.Here,we test the impact on goat metabolism of varying leaf pro...Background:To optimize animal production systems for future sustainability,research now focuses on improving feed quality to enhance livestock performance.Here,we test the impact on goat metabolism of varying leaf proportions in the alfalfa component of an alfalfa-maize diet.Methods:In a pen-feeding experiment,16 three-month-old goats were divided into eight groups,four of which were fed a ration containing 40%alfalfa with leaves intact(LI)and four of which were fed an otherwise identical diet containing stemmy alfalfa(leaves removed,LR).Results:As expected,LR reduced protein intake compared to LI(48.3 vs.93.3 g d^(-1);p<0.001)and increased acid detergent fiber intake(239 vs.167 g d^(-1);p<0.001),but neutral detergent fiber and dry matter digestibility did not differ between LR and LI.The LR group had lower gross energy,digestible energy,and metabolizable energy intakes than the LI group(p=0.080,0.030,and 0.022,respectively).Retained nitrogen for LI and LR was 2.30 and 1.72 g d^(-1),respectively.Body weight gain was 74 g d^(-1)in LI and 52 g d^(-1)in LR(p<0.001).Conclusions:These results highlight that even where alfalfa is only one component of a mixed diet,the nutritive quality and leaf content of the alfalfa component are important to animal performance.展开更多
基金Supported by Technical Development Project of Provincial Scientific Research Institutes in Hainan(KYYS-2016-12)
文摘[Objective] The paper was to study the effects on growth performance and feed digestibility of Hainan black pigs by adding lysine in low prntein diet. [Method] A tntal of 36 Hainan black pigs with the body weight of （46.34±3.06） kg were randomly divided into three groups： con- trol group （protein level 15%, lysine level 0.83%）, experimental grnup 1 （protein level 13%, 0.83%）, and experimental group II （protein level 13%, lysine level 1.03）, each group had three replicates of four pigs. The trial lasted 35 d. [Result] The growth performance of Hainan black pig in grnup II had no significan! difference with that in contrail group （P〉0.05）. The apparent digestibility of chide protein and phosphorus of Hainan black pigs in Group II increased significantly （P〈0.05）, which were increased by 10.08% and 11.85% respectively compared to the control group; the apparent digestibility of calcium was increased, but the difference was not signifieant （P〉0.05）. [Conclusion] It will not reduce the growth performance of Hainan blaek pig when the protein level of diet is reduced by 2% and Ihe lysine level is increased to 1.03%. It is recommended that the diet can be used in the production of llainan black pig.
文摘Three goats were fed with ^15N-labelled rice straw to study the characteristics of digestion,assimilation,transformation and excretion of C and N compounds from rice straw.It was shown that the amount of ^15N transformed into the bodies of the two slaughtered goats accounted for 38.5 and 23.6% of the total amount of ^15N deposition of the experimental diet taken by each goat.The ^15N excreted through the feces and urine for the three goats accounted for 34.8,33.2 and 33.9% of the total amount of ^15N deposition in the feed of the 3 goats.The recovery of total ^15N for the two slaughtered goats were 73.3 and 57.5%,with the corresponding rates of ^15N loss 26.7 and 42.5% respectively.The digestibilities of total amino acids for Goats 1 and 3 were 68.7 and 54.0%,and the digestibilties of carbohydrates for the two goats were 74.8 and 67.7% respectively.
基金funded by a grant from the National Science and Technology Major Projects of China (2014ZX08006004)three grants from the Department of Science and Technology of Guangdong,China (20111090700016,2011A020102003 and 2011A020201009)
文摘Many animal feed grains contain high β-glucan in the cell wall. Pigs do not secret β-glucanase to degrade the β-glucan in their feed. The indigestible β-glucan not only blocks the release of nutrients from the grain cell wall, but also increases the digesta viscosity in the gastrointestinal tract of pigs. Therefore, dietary β-glucan significantly inhibits nutrient digestion and absorption in pigs. Transgenic expression of β-glucanase in the digestive tract of pigs may offer a solution to solve this problem. In the current study, four artificial codon-optimized β-glucanases genes was prepared and expressed in porcine cells. Only p Bg A and p Egx showed high activity in transfected pig kidney cells. To improve the p H range and p H stability of β-glucanase, the two β-glucanases, p Bg A and p Egx, were co-expressed in pig kidney cells and salivary gland cells by Linker A3 or 2A peptide. The resulting dual enzymes of p Bg A3 p Eg and p Bg2 Ap Eg showed significantly enlarged p H range and significantly increased p H stability, as compared to parental enzymes. These results provide useful data for future study on increasing the feed digestibility of pigs by transgenic expression of β-glucanase in their salivary glands.
基金The Key Research and Development Program of Gansu Province,Grant/Award Number:23ZDWA002ACIAR project,Grant/Award Number:LWR/2007/191+4 种基金The mechanism of important functional native grasses in the Natural Grasslands of China and Mongolia on the Digestion,Metabolism and Methane Emission of Sheep,Grant/Award Number:32161143028Construction mode and key technology of grassland ecological civilization demonstration area in Ningxia,Grant/Award Number:2021NXLCZDYF03The Innovative Research Team of the Ministry of Education,Grant/Award Number:IRT_17R50Gansu Forestry and Grassland Administration-Grassland monitoring and evaluation technology support project of Gansu Province,Grant/Award Number:2022-794The Lanzhou city's scientific research funding subsidy to Lanzhou university,Grant/Award Number:GRCZC2021001。
文摘Background:To optimize animal production systems for future sustainability,research now focuses on improving feed quality to enhance livestock performance.Here,we test the impact on goat metabolism of varying leaf proportions in the alfalfa component of an alfalfa-maize diet.Methods:In a pen-feeding experiment,16 three-month-old goats were divided into eight groups,four of which were fed a ration containing 40%alfalfa with leaves intact(LI)and four of which were fed an otherwise identical diet containing stemmy alfalfa(leaves removed,LR).Results:As expected,LR reduced protein intake compared to LI(48.3 vs.93.3 g d^(-1);p<0.001)and increased acid detergent fiber intake(239 vs.167 g d^(-1);p<0.001),but neutral detergent fiber and dry matter digestibility did not differ between LR and LI.The LR group had lower gross energy,digestible energy,and metabolizable energy intakes than the LI group(p=0.080,0.030,and 0.022,respectively).Retained nitrogen for LI and LR was 2.30 and 1.72 g d^(-1),respectively.Body weight gain was 74 g d^(-1)in LI and 52 g d^(-1)in LR(p<0.001).Conclusions:These results highlight that even where alfalfa is only one component of a mixed diet,the nutritive quality and leaf content of the alfalfa component are important to animal performance.
