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Conventional Multiplex PCR for Identifying Soil Transmitted Helminthes (Ascaris Lumbricoides, Trichuris Trichura and Ancylostoma Duodenale) in Fecal Specimens
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作者 Ghada Basi Ali Alomashi Hasan Raheem Khudhur Layla Safar Jebur 《Nano Biomedicine & Engineering》 2022年第4期289-294,共6页
Ascaris lumbricoides, Trichuris trichiura, and Ancylostoma duodenale are soil-transmitted helminths (STHs) and medically neglected in Iraq country in spite of their effect on the public health. A cross-sectional study... Ascaris lumbricoides, Trichuris trichiura, and Ancylostoma duodenale are soil-transmitted helminths (STHs) and medically neglected in Iraq country in spite of their effect on the public health. A cross-sectional study was performed in the Maternity and Childhood Teaching Hospital and General Education Hospital in Al-Dewanyia Province, included 850 tool samples collected from patients who attended to the O&P Lab. General stool examination (GSE), direct wet mount method DWMM and Kato-Katz were using for diagnosis of STH infections through detecting the adult and the ovum of the helminthes. A conventional multiplex PCR assay was used for detection of STHs in fecal samples. Based on microscopic examination, the results showed that 275/850 range among triple, double and single infection on other hand was 365/850 range among triple, double and single infection. In conclusions, the investigative sensitivity of DWMM is notable for STH;in exception, it is capable to identify patients with the intention of highest requirement of management, and therefore contributes to the universal target to reduce STH as a community healthiness trouble. 展开更多
关键词 Multiplex PCR Ascais lumbricoides Trichuris trichura Ancylostoma duodenale fecal specimens
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