Four kinds of assays were used to study the effect of a fat-soluble extract of spinach powder (SPFE) on the proliferation of human gastric adenocareinoma cell line (SGC-7901) in vitro.These studies included: (Ⅰ) cell...Four kinds of assays were used to study the effect of a fat-soluble extract of spinach powder (SPFE) on the proliferation of human gastric adenocareinoma cell line (SGC-7901) in vitro.These studies included: (Ⅰ) cell growth assay, (Ⅱ) colony forming assay, (Ⅲ) MTT colorimetric assay, and (Ⅳ) 3H-TdR incorporation assay. The concentrations of SPFE expressed as the level of β-carotene in the medium were 2×10-8, 2×10-7 and 2×10-6 mol/L β-carotene in assays (Ⅰ)～(Ⅲ), but 4×10- 8, 4×10-7 and 4×10-6 mol/L β-caretene in assay (Ⅳ) respectively. The results indicated that SPFE inhibited the prolifendion and colony forming ability of SGC-7901 cells. And in MTT assay, SPFE inhibited the viability of SGC7901 cells, but no inhibitory effect of SPFE was observed on the viability of lymphocytes in peripheral blood of healthy people. Finally, in the 3H-TdR incorporation test, both SPFE and β-carotene showed significant inhibitory effects on DNA synthesis in SGC-7901 cells, but SPFE was more effective than β-carotene.展开更多
Objective:To study the effect of fat-soluble vitamins combined with antibiotic therapy on serum indexes and pulmonary function of acute infantile mycoplasma pneumoniae infection. Methods:A total of 124 children with m...Objective:To study the effect of fat-soluble vitamins combined with antibiotic therapy on serum indexes and pulmonary function of acute infantile mycoplasma pneumoniae infection. Methods:A total of 124 children with mycoplasma pneumoniae pneumonia treated in our hospital between May 2014 and March 2016 were selected as the research subjects and randomly divided into two groups, vitamin group received fat-soluble vitamins combined with antibiotic therapy and control group accepted routine antibiotic therapy. 4 weeks after treatment, serum was collected and the kits were used to detect the content of inflammatory cytokines and antioxidants, and spirometer was used on the same day to detect lung function parameters.Results:Serum ICAM-1, IL-5, IL-17A, IL-18 and TNF-α content of vitamin group after treatment were significantly lower than those before treatment while CAT, MPO, SOD and GSH-Px content were significantly higher than those before treatment;serum ICAM-1, IL-5, IL-17A, IL-18, TNF-α, CAT, MPO, SOD and GSH-Px content of control group after treatment were significantly lower than those before treatment;after treatment, serum ICAM-1, IL-5, IL-17A, IL-18 and TNF-α content of vitamin group were significantly lower than those of control group while CAT, MPO, SOD and GSH-Px content were significantly higher than those of control group, and lung function parameters FVC, FEV1, PEF, FEF25, FEF50 and FEF75 were significantly higher than those of control group. Conclusion:The fat-soluble vitamins combined with antibiotic treatment of acute infantile mycoplasma pneumoniae infection can inhibit inflammation and oxidative stress, and improve lung function.展开更多
Objective: To evaluate the general conditions and inflammatory response of antibiotics combined with fat-soluble vitamin therapy for pediatric pneumonia. Methods: A total of 350 children with pneumonia who were treate...Objective: To evaluate the general conditions and inflammatory response of antibiotics combined with fat-soluble vitamin therapy for pediatric pneumonia. Methods: A total of 350 children with pneumonia who were treated in our hospital between January 2014 and January 2017 were collected and divided into control group and observation group according to the random number table, 175 cases in each group. Control group received azithromycin therapy, and observation group received azithromycin combined with fat-soluble vitamin therapy. The differences in the systemic and inflammatory responses before and after treatment were compared between the two groups of patients. Results: Before treatment, differences in blood routine index levels as well as serum contents of oxidative stress indexes and inflammatory factors were not statistically significant between two groups of patients. After treatment, white blood cell count, neutrophil ratio as well as serum CRP, PCT, IL-6 and TNF-α contents of both groups were lower than those before treatment while lymphocyte ratio as well as serum IgA, IgG and IgM contents were higher than those before treatment, and white blood cell count, neutrophil ratio as well as serum CRP, PCT, IL-6 and TNF-α contents of observation group after treatment were lower than those of control group while lymphocyte ratio as well as serum IgA, IgG and IgM contents was higher than those of control group. Conclusion:Antibiotics combined with fat-soluble vitamin therapy for pediatric pneumonia can effectively optimize the patient's general conditions and reduce the inflammatory response.展开更多
Micronutrients are fundamental to support and maintain normal physiological function.Deficiencies of these nutrients are a growing public health concern with potentially devastating consequences.An adequate diet of wh...Micronutrients are fundamental to support and maintain normal physiological function.Deficiencies of these nutrients are a growing public health concern with potentially devastating consequences.An adequate diet of whole foods is the primary source of micronutrients;supplementation is sometimes necessary.Both deficiency and excess of these nutrients have adverse effects.Common deficiencies include iron,folate,iodine,zinc,and vitamin A,which can present clinically as a syndrome.Micronutrient deficiencies(MNDs)are common contributors to intellectual impairments,poor growth,perinatal complications,and increased risk for morbidity and mortality.Excess of a select few of these nutrients can result in conditions such as idiopathic intracranial hypertension and diarrhea.Interventions,including supplementation,fortification,and biofortification,can help combat MNDs.This article reviews some common micronutrient imbalances,their clinical manifestations,and treatment interventions.展开更多
The edible mushroom Agaricus bisporus L.plays a crucial ecological role in nutrient cycling and organic matter decomposition,alongside its increasing importance in the food and nutrition industry.This study explored e...The edible mushroom Agaricus bisporus L.plays a crucial ecological role in nutrient cycling and organic matter decomposition,alongside its increasing importance in the food and nutrition industry.This study explored ecological interventions to enhance the mushroom’s vitamin content by enriching its cultivation substrate with nanomaterials and biostimulatory agents.The experiment was conducted within the mushroom production project at Al-Qadisiyah Governorate,Iraq.The compost-based medium was amended with magnetic iron nanoparticles(N-FeO),carbon nanotube(CNT)suspensions,EM biofertilizer,and Atonik growth stimulant.Their ecological impact on the enrichment of fat-soluble(A,D,E)and water-soluble(B2,B3,B5,B6)vitamins in mushrooms was assessed.The study employed a Completely Randomized Design(CRD)with three replicates.Results revealed that the synergistic application of these eco-friendly treatments significantly enhanced the vitamin profiles of A.bisporus.The highest concentrations of vitamins B2 and B5(5.16 and 17.70 mg kg^(-1),respectively)and vitamin A(6.87 IU ml^(-1))were recorded under the combined quadruple treatment.Additionally,the triple treatment(N-FeO+EM+Atonik)notably increased levels of vitamins B2(4.47 mg kg^(-1)),B6(25.66 mg kg^(-1)),D(34.76 mg kg^(-1)),and vitamin A(6.87 IU ml^(-1)).Dual treatments(EM+Atonik)also significantly improved vitamin B2(4.54 mg kg^(-1))and vitamin E(3.30 mg kg^(-1))contents.These findings demonstrate that integrating nanomaterials and biostimulants can serve as an ecological strategy to improve the nutritional quality of mushrooms while promoting sustainable agricultural practices.展开更多
Primary biliary cirrhosis (PBC) and primary sclerosing cho-langitis (PSC) are two major types of chronic cholestatic liver disease. Each disorder has distinguishing features and variable progression, but both may ulti...Primary biliary cirrhosis (PBC) and primary sclerosing cho-langitis (PSC) are two major types of chronic cholestatic liver disease. Each disorder has distinguishing features and variable progression, but both may ultimately result in cirrhosis and hepatic failure. The following offers a review of PBC and PSC, beginning with a general overview of disease etiology, pathogenesis, diagnosis, clinical features, natural course, and treatment. In addition to commonly associated manifestations of fatigue, pruritus, and fat-soluble vitamin deficiency, select disease-related topics pertaining to women's health are discussed including metabolic bone disease, hyperlipidemia and cardiovascular risk, and preg-nancy-related issues influencing maternal disease course and birth outcomes. This comprehensive review of PBC and PSC highlights some unique clinical considerations in the care of female patients with cholestatic liver disease.展开更多
文摘Four kinds of assays were used to study the effect of a fat-soluble extract of spinach powder (SPFE) on the proliferation of human gastric adenocareinoma cell line (SGC-7901) in vitro.These studies included: (Ⅰ) cell growth assay, (Ⅱ) colony forming assay, (Ⅲ) MTT colorimetric assay, and (Ⅳ) 3H-TdR incorporation assay. The concentrations of SPFE expressed as the level of β-carotene in the medium were 2×10-8, 2×10-7 and 2×10-6 mol/L β-carotene in assays (Ⅰ)～(Ⅲ), but 4×10- 8, 4×10-7 and 4×10-6 mol/L β-caretene in assay (Ⅳ) respectively. The results indicated that SPFE inhibited the prolifendion and colony forming ability of SGC-7901 cells. And in MTT assay, SPFE inhibited the viability of SGC7901 cells, but no inhibitory effect of SPFE was observed on the viability of lymphocytes in peripheral blood of healthy people. Finally, in the 3H-TdR incorporation test, both SPFE and β-carotene showed significant inhibitory effects on DNA synthesis in SGC-7901 cells, but SPFE was more effective than β-carotene.
文摘Objective:To study the effect of fat-soluble vitamins combined with antibiotic therapy on serum indexes and pulmonary function of acute infantile mycoplasma pneumoniae infection. Methods:A total of 124 children with mycoplasma pneumoniae pneumonia treated in our hospital between May 2014 and March 2016 were selected as the research subjects and randomly divided into two groups, vitamin group received fat-soluble vitamins combined with antibiotic therapy and control group accepted routine antibiotic therapy. 4 weeks after treatment, serum was collected and the kits were used to detect the content of inflammatory cytokines and antioxidants, and spirometer was used on the same day to detect lung function parameters.Results:Serum ICAM-1, IL-5, IL-17A, IL-18 and TNF-α content of vitamin group after treatment were significantly lower than those before treatment while CAT, MPO, SOD and GSH-Px content were significantly higher than those before treatment;serum ICAM-1, IL-5, IL-17A, IL-18, TNF-α, CAT, MPO, SOD and GSH-Px content of control group after treatment were significantly lower than those before treatment;after treatment, serum ICAM-1, IL-5, IL-17A, IL-18 and TNF-α content of vitamin group were significantly lower than those of control group while CAT, MPO, SOD and GSH-Px content were significantly higher than those of control group, and lung function parameters FVC, FEV1, PEF, FEF25, FEF50 and FEF75 were significantly higher than those of control group. Conclusion:The fat-soluble vitamins combined with antibiotic treatment of acute infantile mycoplasma pneumoniae infection can inhibit inflammation and oxidative stress, and improve lung function.
文摘Objective: To evaluate the general conditions and inflammatory response of antibiotics combined with fat-soluble vitamin therapy for pediatric pneumonia. Methods: A total of 350 children with pneumonia who were treated in our hospital between January 2014 and January 2017 were collected and divided into control group and observation group according to the random number table, 175 cases in each group. Control group received azithromycin therapy, and observation group received azithromycin combined with fat-soluble vitamin therapy. The differences in the systemic and inflammatory responses before and after treatment were compared between the two groups of patients. Results: Before treatment, differences in blood routine index levels as well as serum contents of oxidative stress indexes and inflammatory factors were not statistically significant between two groups of patients. After treatment, white blood cell count, neutrophil ratio as well as serum CRP, PCT, IL-6 and TNF-α contents of both groups were lower than those before treatment while lymphocyte ratio as well as serum IgA, IgG and IgM contents were higher than those before treatment, and white blood cell count, neutrophil ratio as well as serum CRP, PCT, IL-6 and TNF-α contents of observation group after treatment were lower than those of control group while lymphocyte ratio as well as serum IgA, IgG and IgM contents was higher than those of control group. Conclusion:Antibiotics combined with fat-soluble vitamin therapy for pediatric pneumonia can effectively optimize the patient's general conditions and reduce the inflammatory response.
文摘Micronutrients are fundamental to support and maintain normal physiological function.Deficiencies of these nutrients are a growing public health concern with potentially devastating consequences.An adequate diet of whole foods is the primary source of micronutrients;supplementation is sometimes necessary.Both deficiency and excess of these nutrients have adverse effects.Common deficiencies include iron,folate,iodine,zinc,and vitamin A,which can present clinically as a syndrome.Micronutrient deficiencies(MNDs)are common contributors to intellectual impairments,poor growth,perinatal complications,and increased risk for morbidity and mortality.Excess of a select few of these nutrients can result in conditions such as idiopathic intracranial hypertension and diarrhea.Interventions,including supplementation,fortification,and biofortification,can help combat MNDs.This article reviews some common micronutrient imbalances,their clinical manifestations,and treatment interventions.
文摘The edible mushroom Agaricus bisporus L.plays a crucial ecological role in nutrient cycling and organic matter decomposition,alongside its increasing importance in the food and nutrition industry.This study explored ecological interventions to enhance the mushroom’s vitamin content by enriching its cultivation substrate with nanomaterials and biostimulatory agents.The experiment was conducted within the mushroom production project at Al-Qadisiyah Governorate,Iraq.The compost-based medium was amended with magnetic iron nanoparticles(N-FeO),carbon nanotube(CNT)suspensions,EM biofertilizer,and Atonik growth stimulant.Their ecological impact on the enrichment of fat-soluble(A,D,E)and water-soluble(B2,B3,B5,B6)vitamins in mushrooms was assessed.The study employed a Completely Randomized Design(CRD)with three replicates.Results revealed that the synergistic application of these eco-friendly treatments significantly enhanced the vitamin profiles of A.bisporus.The highest concentrations of vitamins B2 and B5(5.16 and 17.70 mg kg^(-1),respectively)and vitamin A(6.87 IU ml^(-1))were recorded under the combined quadruple treatment.Additionally,the triple treatment(N-FeO+EM+Atonik)notably increased levels of vitamins B2(4.47 mg kg^(-1)),B6(25.66 mg kg^(-1)),D(34.76 mg kg^(-1)),and vitamin A(6.87 IU ml^(-1)).Dual treatments(EM+Atonik)also significantly improved vitamin B2(4.54 mg kg^(-1))and vitamin E(3.30 mg kg^(-1))contents.These findings demonstrate that integrating nanomaterials and biostimulants can serve as an ecological strategy to improve the nutritional quality of mushrooms while promoting sustainable agricultural practices.
文摘Primary biliary cirrhosis (PBC) and primary sclerosing cho-langitis (PSC) are two major types of chronic cholestatic liver disease. Each disorder has distinguishing features and variable progression, but both may ultimately result in cirrhosis and hepatic failure. The following offers a review of PBC and PSC, beginning with a general overview of disease etiology, pathogenesis, diagnosis, clinical features, natural course, and treatment. In addition to commonly associated manifestations of fatigue, pruritus, and fat-soluble vitamin deficiency, select disease-related topics pertaining to women's health are discussed including metabolic bone disease, hyperlipidemia and cardiovascular risk, and preg-nancy-related issues influencing maternal disease course and birth outcomes. This comprehensive review of PBC and PSC highlights some unique clinical considerations in the care of female patients with cholestatic liver disease.
