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维生素D调节自噬活性和FXR/FGF15信号通路改善大鼠2型糖尿病肾脏功能的作用机制
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作者 赵月萍 刘明 +1 位作者 张奇 张瑞 《川北医学院学报》 2026年第2期129-135,共7页
目的:探究维生素D调节自噬活性和FXR/FGF15信号通路改善大鼠2型糖尿病的作用机制。方法:选取40只SPF级SD大鼠,其中10只大鼠作为对照组,30只构建2型糖尿病模型。将建模成功的27只大鼠分为模型组、维生素D组、维生素D+FXR抑制剂组,每组各... 目的:探究维生素D调节自噬活性和FXR/FGF15信号通路改善大鼠2型糖尿病的作用机制。方法:选取40只SPF级SD大鼠,其中10只大鼠作为对照组,30只构建2型糖尿病模型。将建模成功的27只大鼠分为模型组、维生素D组、维生素D+FXR抑制剂组,每组各9只。比较各组大鼠肾组织病理组织学变化、空腹血糖、胰岛素、体质量、肾功能、炎症因子水平、自噬相关蛋白、FXR/FGF15通路mRNA及蛋白表达水平。结果:对照组大鼠肾组织结构正常;模型组大鼠肾小球肥大、系膜细胞增生,肾小管上皮细胞肿胀变性且部分萎缩;维生素D组大鼠肾病理损伤减轻,而维生素D+FXR抑制剂组大鼠损伤较维生素D组加重,程度介于模型组与维生素D组之间。与对照组相比,模型组大鼠空腹血糖、肌酐(Scr)、尿素氮(BUN)、单核细胞趋化蛋白1(MCP-1)、白细胞介素6(IL-6)、白细胞介素1β(IL-1β)、肿瘤坏死因子ɑ(TNF-ɑ)、P62蛋白表达升高;胰岛素、体质量、溶酶体相关膜蛋白1(LAMP1)、LC3-Ⅱ/Ⅰ蛋白、法尼醇X受体(FXR)、成纤维细胞生长因子15(FGF15)mRNA、蛋白表达水平降低,差异均有统计学意义(P<0.05)。与模型组相比,维生素D组大鼠空腹血糖、Scr、BUN、MCP-1、IL-6、IL-1β、TNF-ɑ、P62蛋白表达降低,胰岛素、体质量、LAMP1、LC3-Ⅱ/Ⅰ蛋白、FXR、FGF15 mRNA、蛋白表达水平升高(P<0.05)。与维生素D组相比,维生素D+FXR抑制剂组大鼠空腹血糖、Scr、BUN、MCP-1、IL-6、IL-1β、TNF-ɑ、P62蛋白表达升高;胰岛素、体质量、LAMP1、LC3-Ⅱ/Ⅰ蛋白、FXR、FGF15 mRNA、蛋白表达水平降低,差异均有统计学意义(P<0.05)。结论:维生素D可改善肾功能,作用机制可能与调节自噬活性和FXR/FGF15通路有关。 展开更多
关键词 2型糖尿病 维生素D 法尼酯X受体/成纤维细胞生长因子 自噬活性 肾脏功能
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气质联用追踪筛选辛夷挥发油提取工艺 被引量:7
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作者 魏刚 赵敏华 +1 位作者 方春亮 柯雪红 《时珍国医国药》 CAS CSCD 2000年第2期105-106,共2页
采用GC-MS为追踪手段,确定辛夷挥发油提取优选工艺为:辛夷粗粉采用水蒸气蒸馏法提取3~4h,提取率约为86%。
关键词 辛夷 挥发油 提取方法 GC-MS 金合欢醇 中药
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A Unifying Theory of Dark Energy, Dark Matter, and Baryonic Matter in the Positive-Negative Mass Universe Pair: Protogalaxy and Galaxy Evolutions
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作者 Ding-Yu Chung 《Journal of Modern Physics》 2020年第7期1091-1122,共32页
This paper modifies the Farnes’ unifying theory of dark energy and dark matter which are negative-mass, created continuously from the negative-mass universe in the positive-negative mass universe pair. The first modi... This paper modifies the Farnes’ unifying theory of dark energy and dark matter which are negative-mass, created continuously from the negative-mass universe in the positive-negative mass universe pair. The first modification explains that observed dark energy is 68.6%, greater than 50% for the symmetrical positive-negative mass universe pair. This paper starts with the proposed positive-negative-mass 11D universe pair (without kinetic energy) which is transformed into the positive-negative mass 10D universe pair and the external dual gravities as in the Randall-Sundrum model, resulting in the four equal and separate universes consisting of the positive-mass 10D universe, the positive-mass massive external gravity, the negative-mass 10D universe and the negative-mass massive external gravity. The positive-mass 10D universe is transformed into 4D universe (home universe) with kinetic energy through the inflation and the Big Bang to create positive-mass dark matter which is five times of positive-mass baryonic matter. The other three universes without kinetic energy oscillate between 10D and 10D through 4D, resulting in the hidden universes when D > 4 and dark energy when D = 4, which is created continuously to our 4D home universe with the maximum dark energy = 3/4 = 75%. In the second modification to explain dark matter in the CMB, dark matter initially is not repulsive. The condensed baryonic gas at the critical surface density induces dark matter repulsive force to transform dark matter in the region into repulsive dark matter repulsing one another. The calculated percentages of dark energy, dark matter, and baryonic matter are 68.6 (as an input from the observation), 26 and 5.2, respectively, in agreement with observed 68.6, 26.5 and 4.9, respectively, and dark energy started in 4.33 billion years ago in agreement with the observed 4.71 <span style="white-space:nowrap;">&plusmn;</span> 0.98 billion years ago. In conclusion, the modified Farnes’ unifying theory reinterprets the Farnes’ equations, and is a unifying theory of dark energy, dark matter, and baryonic matter in the positive-negative mass universe pair. The unifying theory explains protogalaxy and galaxy evolutions in agreement with the observations. 展开更多
关键词 Unifying Theory farnes Dark Energy Dark Matter Baryonic Matter Negative Mass Positive-Negative Mass Universe Pair Protogalaxy Evolution Galaxy Evolution
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