The cDNA of PHYC gene of tall fescue ( Festuca arundinacea ) was cloned by RT-PCR,named FaPHYC.The sequence was analyzed by bioinformatic software,and the results showed that the full-length cDNA(3816 bp) was obtained...The cDNA of PHYC gene of tall fescue ( Festuca arundinacea ) was cloned by RT-PCR,named FaPHYC.The sequence was analyzed by bioinformatic software,and the results showed that the full-length cDNA(3816 bp) was obtained with an open reading frame predicated to be 3 411 bp,which encoded 1 136 amino acids.By BLASTP analysis,the N-terminal of FaPHYC was composed of GAF and pPhytochrome domains,and its C-terminal included two repeated PAS domains,one histidine kinase A domain and one histidine kinase-like ATPase domain.The phylogenetic analysis showed that FaPHYC was closely related to PHYCs from other Gramineae plants.The qRT-PCR results showed that FaPHYC expression was regulated by drought stress,heat stress,salt stress and nitrogen stress.The real-time quantitative PCR assay showed the expression of FaPHYC in tall fescue seedlings was continuously up-regulated during the light period and down-regulated in the dark.The results suggest that FaPHYC is photoperiod sensitive and plays a functional role in flowering regulation by photoperiod in tall fescue.The FaPHYC gene was cloned and reported from tall fescue for the first time,which lays a foundation for the investigation of the stress mechanism of FaPHYC in tall fescue plant.展开更多
基金Supported by project of High-level Innovative Talents Project in Guizhou Province([2018]5634)Youth Fund of Guizhou Academy of Agricultural Sciences(2018-80)Guizhou Province S&T Contract Basics([2019]1302)
文摘The cDNA of PHYC gene of tall fescue ( Festuca arundinacea ) was cloned by RT-PCR,named FaPHYC.The sequence was analyzed by bioinformatic software,and the results showed that the full-length cDNA(3816 bp) was obtained with an open reading frame predicated to be 3 411 bp,which encoded 1 136 amino acids.By BLASTP analysis,the N-terminal of FaPHYC was composed of GAF and pPhytochrome domains,and its C-terminal included two repeated PAS domains,one histidine kinase A domain and one histidine kinase-like ATPase domain.The phylogenetic analysis showed that FaPHYC was closely related to PHYCs from other Gramineae plants.The qRT-PCR results showed that FaPHYC expression was regulated by drought stress,heat stress,salt stress and nitrogen stress.The real-time quantitative PCR assay showed the expression of FaPHYC in tall fescue seedlings was continuously up-regulated during the light period and down-regulated in the dark.The results suggest that FaPHYC is photoperiod sensitive and plays a functional role in flowering regulation by photoperiod in tall fescue.The FaPHYC gene was cloned and reported from tall fescue for the first time,which lays a foundation for the investigation of the stress mechanism of FaPHYC in tall fescue plant.
