The 3,5-dicaffeoylquinic acid(3,5-DiCQA),a key component of Chuzhou chrysanthemum,one of the“Four Major Edible Chrysanthemums”in China,has been shown to enrich beneficial gut bacteria and reduce lipid synthesis base...The 3,5-dicaffeoylquinic acid(3,5-DiCQA),a key component of Chuzhou chrysanthemum,one of the“Four Major Edible Chrysanthemums”in China,has been shown to enrich beneficial gut bacteria and reduce lipid synthesis based on its hydroxycinnamic acid structures.However,its low lipophilicity limits bioactivity,necessitating the development of more stable derivatives.Therefore,this study synthesized and characterized a novel esterified derivative 3,5-DiCQA dodecyl ester(3,5-DiCQAD)with markedly enhanced lipophilicity and bioactivity.In highfat diet(HFD)-induced male C57BL/6J mice,3,5-DiCQAD showed remarkable metabolic regulatory effects,including improvements in glucose metabolism,lipid homeostasis,BA signaling,and thermogenesis.Furthermore,3,5-DiCQAD effectively reshaped the gut microbiota,enriching beneficial bacteria such as Lactobacillus plantarum.Moreover,the strain L.plantarum ZZ 240321 was isolated,and its fermentation of 3,5-DiCQAD generated metabolites that modulated bile acid(BA)metabolism through the FXR/SHP/LRH-1 pathway,alleviating obesity-related symptoms in antibiotic-treated mice.This research provides a novel perspective for developing plant-derived polyphenols as anti-obesity treatments.It also broadens the understanding of DiCQA’s probiotic potential in the gut microbiome,highlighting its promising role in managing metabolism-associated disorders.展开更多
Shuiman tea,a unique variant of Pu-erh tea,contains polysaccharides known for their antioxidant and hypoglycemic activities.However,their anti-obesity potential remains unexplored.In this study,a homogeneous acidic po...Shuiman tea,a unique variant of Pu-erh tea,contains polysaccharides known for their antioxidant and hypoglycemic activities.However,their anti-obesity potential remains unexplored.In this study,a homogeneous acidic polysaccharide,STPS-3-1,was successfully isolated from Shuiman tea,and its preliminary structure and digestion characteristics were comprehensively analyzed.After 12 weeks of STPS-3-1 intervention,high-fat diet(HFD)-induced male C57BL/6J mice(specific pathogen free(SPF)grade,6 weeks old,18–20 g)exhibited significant metabolic improvements,including improved blood glucose and lipid levels,mitigated metabolic disorders,and reduced inflammation.Furthermore,STPS-3-1 notably reshaped the gut microbiota,enriching key species such as Bacteroides vulgatus.Subsequently,the B.vulgatus LWZ 240601 strain was successfully isolated and identified.This strain efficiently degraded STPS-3-1 under the synergistic action of three polysaccharide utilization loci(PULs),producing metabolites that regulated bile acid(BA)metabolism through the FXR/SHP signaling pathway,significantly alleviating obesity-related symptoms in antibiotic(ABX)-treated mice.This study provides valuable insights into the metabolic regulatory mechanisms and anti-obesity potential of STPS-3-1,elucidating their probiotic effects through gut microbiota modulation.Additionally,it expands the potential of tea polysaccharides in gut microbiota modulation and metabolic disease intervention,supporting the development of plant-based functional foods.展开更多
法尼醇X受体(Farnesoid X Receptor,FXR)属于代谢性核受体,是需配体激活的转录因子,在肝脏胆汁酸、脂质代谢过程,肝脏炎症和肿瘤的发展过程中起着重要的调节作用。小异二聚体伴侣受体(Small Heterodimer Partner,SHP)是核受体超家族中...法尼醇X受体(Farnesoid X Receptor,FXR)属于代谢性核受体,是需配体激活的转录因子,在肝脏胆汁酸、脂质代谢过程,肝脏炎症和肿瘤的发展过程中起着重要的调节作用。小异二聚体伴侣受体(Small Heterodimer Partner,SHP)是核受体超家族中的一个特殊成员,在特异的组织中作为转录调节的共抑制因子,抑制其他多种转录因子的活性,在众多代谢通路中起到了负性调节作用。近年来研究发现,核受体FXR通过对SHP的调控来实现其在肝脏的多种功能。本文着重对FXR调节SHP的机制及FXR-SHP轴在肝脏中作用进行综述。展开更多
基金supported by the National Natural Science Foundation of China(No.31901617)the National Key Research and Development Program of China(2017YFD0400600)+8 种基金the Top Talent Project of Department of Education of Anhui Province(gxyqZD2021125)the Excellent Talent project of Anhui Science and Technology University(XJYXRC202101)the Stable Talent Personnel Project of Anhui Science and Technology University(SPWD202101)the Natural Science Research Foundation of Department of Education of Anhui Province(2022AH040236)Project of Science and Technology Innovation Team of Anhui Science and Technology University(2023KJCXTD003)the Undergraduate innovation and entrepreneurship training program of Anhui Province(202410879088)the Excellent Research and Innovation Team of Anhui Province(2024AH010007)Discipline Leader Development Program of Anhui Province(DTR2024034)Anhui Province Youth Top Talent Scholar Program.
文摘The 3,5-dicaffeoylquinic acid(3,5-DiCQA),a key component of Chuzhou chrysanthemum,one of the“Four Major Edible Chrysanthemums”in China,has been shown to enrich beneficial gut bacteria and reduce lipid synthesis based on its hydroxycinnamic acid structures.However,its low lipophilicity limits bioactivity,necessitating the development of more stable derivatives.Therefore,this study synthesized and characterized a novel esterified derivative 3,5-DiCQA dodecyl ester(3,5-DiCQAD)with markedly enhanced lipophilicity and bioactivity.In highfat diet(HFD)-induced male C57BL/6J mice,3,5-DiCQAD showed remarkable metabolic regulatory effects,including improvements in glucose metabolism,lipid homeostasis,BA signaling,and thermogenesis.Furthermore,3,5-DiCQAD effectively reshaped the gut microbiota,enriching beneficial bacteria such as Lactobacillus plantarum.Moreover,the strain L.plantarum ZZ 240321 was isolated,and its fermentation of 3,5-DiCQAD generated metabolites that modulated bile acid(BA)metabolism through the FXR/SHP/LRH-1 pathway,alleviating obesity-related symptoms in antibiotic-treated mice.This research provides a novel perspective for developing plant-derived polyphenols as anti-obesity treatments.It also broadens the understanding of DiCQA’s probiotic potential in the gut microbiome,highlighting its promising role in managing metabolism-associated disorders.
基金supported by the National Natural Science Foundation of China(No.31901617)the National Key Research and Development Program of China(2017YFD0400600)+8 种基金the Top Talent Project of Department of Education of Anhui Province(gxyqZD2021125)the Excellent Talent project of Anhui Science and Technology University(XJYXRC202101)the Stable Talent Personnel Project of Anhui Science and Technology University(SPWD202101)the Natural Science Research Foundation of Department of Education of Anhui Province(2022AH040236)Project of Science and Technology Innovation Team of Anhui Science and Technology University(2023KJCXTD003)the Undergraduate Innovation and Entrepreneurship Training Program of Anhui Province(S202310879232)the Excellent Research and Innovation Team of Anhui Province Universities(2024AH010007)Discipline Leader Development Program of Anhui Province(DTR2024034)Anhui Province Youth Top Talent Scholar Program.
文摘Shuiman tea,a unique variant of Pu-erh tea,contains polysaccharides known for their antioxidant and hypoglycemic activities.However,their anti-obesity potential remains unexplored.In this study,a homogeneous acidic polysaccharide,STPS-3-1,was successfully isolated from Shuiman tea,and its preliminary structure and digestion characteristics were comprehensively analyzed.After 12 weeks of STPS-3-1 intervention,high-fat diet(HFD)-induced male C57BL/6J mice(specific pathogen free(SPF)grade,6 weeks old,18–20 g)exhibited significant metabolic improvements,including improved blood glucose and lipid levels,mitigated metabolic disorders,and reduced inflammation.Furthermore,STPS-3-1 notably reshaped the gut microbiota,enriching key species such as Bacteroides vulgatus.Subsequently,the B.vulgatus LWZ 240601 strain was successfully isolated and identified.This strain efficiently degraded STPS-3-1 under the synergistic action of three polysaccharide utilization loci(PULs),producing metabolites that regulated bile acid(BA)metabolism through the FXR/SHP signaling pathway,significantly alleviating obesity-related symptoms in antibiotic(ABX)-treated mice.This study provides valuable insights into the metabolic regulatory mechanisms and anti-obesity potential of STPS-3-1,elucidating their probiotic effects through gut microbiota modulation.Additionally,it expands the potential of tea polysaccharides in gut microbiota modulation and metabolic disease intervention,supporting the development of plant-based functional foods.
文摘法尼醇X受体(Farnesoid X Receptor,FXR)属于代谢性核受体,是需配体激活的转录因子,在肝脏胆汁酸、脂质代谢过程,肝脏炎症和肿瘤的发展过程中起着重要的调节作用。小异二聚体伴侣受体(Small Heterodimer Partner,SHP)是核受体超家族中的一个特殊成员,在特异的组织中作为转录调节的共抑制因子,抑制其他多种转录因子的活性,在众多代谢通路中起到了负性调节作用。近年来研究发现,核受体FXR通过对SHP的调控来实现其在肝脏的多种功能。本文着重对FXR调节SHP的机制及FXR-SHP轴在肝脏中作用进行综述。
文摘为研究功效性米粉对高脂诱导小鼠高血脂症的防治作用,并探讨其作用机制,选取决明子、葛根、山药、薏苡仁、沙棘等药食同源原料,与荞麦和藜麦混合制成米粉,选取48只六周龄无特定病原体(specific pathogen free,SPF)级健康ICR雄性小鼠,采用高脂饲料构建高血脂模型,并在造模期间灌胃功效性米粉干预。检测每组小鼠体质量、肝质量变化,血清中甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)含量,以及血清、肝脏、粪便中总胆汁酸含量,肝脏组织中法尼酯X受体(farnesoid X receptor,FXR)蛋白、小异二聚体伴侣(small heterodimer partner,SHP)蛋白、细胞色素P450家族成员7A1(cytochrome P4507A1,CYP7A1)蛋白表达水平和肠道菌群结构以及多样性。结果表明,功效性米粉能有效降低高血脂小鼠血清TG、TC和LDL-C含量,提高HDL-C含量;降低血清和肝脏中总胆汁酸含量,提高粪便中总胆汁酸含量;同时下调肝脏中FXR、SHP蛋白的表达;提高肠道微生物的丰度和多样性,改善肠道菌群结构。因此,功效性米粉可以防治高脂诱导小鼠的高血脂症,调节肠道菌群,其机制可能与调节FXR-SHP通路和促进胆汁酸排泄有关。
