Alcohol intake is associated with increased mortality worldwide,particularly liver diseases,making it imperative to explore innovative strategies for managing alcohol-related liver disease.In this study,t he efficacy ...Alcohol intake is associated with increased mortality worldwide,particularly liver diseases,making it imperative to explore innovative strategies for managing alcohol-related liver disease.In this study,t he efficacy of Scytosiphon lomentaria fucoidan(SLF)in alleviating alcohol-induced liver injury was evaluated in a mouse model.It showed that SLF increased body weight and colon length,while reducing liver index,serum lipid,alanine aminotransferase,and aspartate aminotransferase in alcohol-treated mice.SLF inhibited inflammatory response in the liver by reducing inflammatory infiltration and the levels of pro-inflammatory cytokines.It can be associated with the alleviation of oxidative stress and the inhibition of the nuclear factor-κB pathway.SLF modulated alcohol-induced dysbiosis of gut microbiota,including a reduction in Bacteroidetes and Proteobacteria,and improved metabolites profile,primarily affecting short chain fatty acids and amino acids metabolism.In addition,SLF reduced the level of total bile acids,regulated the profile of bile acids,and increased the levels of farnesoid X receptor(FXR)and AMP-activated protein kinase(AMPK),suggesting that SLF can alleviate alcohol-induced liver injury by regulating bile acid-FXR/AMPK pathway.This study suggests that SLF holds the potential to alleviate the adverse effect of alcohol on the liver via the gut-liver axis.展开更多
The 3,5-dicaffeoylquinic acid(3,5-DiCQA),a key component of Chuzhou chrysanthemum,one of the“Four Major Edible Chrysanthemums”in China,has been shown to enrich beneficial gut bacteria and reduce lipid synthesis base...The 3,5-dicaffeoylquinic acid(3,5-DiCQA),a key component of Chuzhou chrysanthemum,one of the“Four Major Edible Chrysanthemums”in China,has been shown to enrich beneficial gut bacteria and reduce lipid synthesis based on its hydroxycinnamic acid structures.However,its low lipophilicity limits bioactivity,necessitating the development of more stable derivatives.Therefore,this study synthesized and characterized a novel esterified derivative 3,5-DiCQA dodecyl ester(3,5-DiCQAD)with markedly enhanced lipophilicity and bioactivity.In highfat diet(HFD)-induced male C57BL/6J mice,3,5-DiCQAD showed remarkable metabolic regulatory effects,including improvements in glucose metabolism,lipid homeostasis,BA signaling,and thermogenesis.Furthermore,3,5-DiCQAD effectively reshaped the gut microbiota,enriching beneficial bacteria such as Lactobacillus plantarum.Moreover,the strain L.plantarum ZZ 240321 was isolated,and its fermentation of 3,5-DiCQAD generated metabolites that modulated bile acid(BA)metabolism through the FXR/SHP/LRH-1 pathway,alleviating obesity-related symptoms in antibiotic-treated mice.This research provides a novel perspective for developing plant-derived polyphenols as anti-obesity treatments.It also broadens the understanding of DiCQA’s probiotic potential in the gut microbiome,highlighting its promising role in managing metabolism-associated disorders.展开更多
Shuiman tea,a unique variant of Pu-erh tea,contains polysaccharides known for their antioxidant and hypoglycemic activities.However,their anti-obesity potential remains unexplored.In this study,a homogeneous acidic po...Shuiman tea,a unique variant of Pu-erh tea,contains polysaccharides known for their antioxidant and hypoglycemic activities.However,their anti-obesity potential remains unexplored.In this study,a homogeneous acidic polysaccharide,STPS-3-1,was successfully isolated from Shuiman tea,and its preliminary structure and digestion characteristics were comprehensively analyzed.After 12 weeks of STPS-3-1 intervention,high-fat diet(HFD)-induced male C57BL/6J mice(specific pathogen free(SPF)grade,6 weeks old,18–20 g)exhibited significant metabolic improvements,including improved blood glucose and lipid levels,mitigated metabolic disorders,and reduced inflammation.Furthermore,STPS-3-1 notably reshaped the gut microbiota,enriching key species such as Bacteroides vulgatus.Subsequently,the B.vulgatus LWZ 240601 strain was successfully isolated and identified.This strain efficiently degraded STPS-3-1 under the synergistic action of three polysaccharide utilization loci(PULs),producing metabolites that regulated bile acid(BA)metabolism through the FXR/SHP signaling pathway,significantly alleviating obesity-related symptoms in antibiotic(ABX)-treated mice.This study provides valuable insights into the metabolic regulatory mechanisms and anti-obesity potential of STPS-3-1,elucidating their probiotic effects through gut microbiota modulation.Additionally,it expands the potential of tea polysaccharides in gut microbiota modulation and metabolic disease intervention,supporting the development of plant-based functional foods.展开更多
文摘Alcohol intake is associated with increased mortality worldwide,particularly liver diseases,making it imperative to explore innovative strategies for managing alcohol-related liver disease.In this study,t he efficacy of Scytosiphon lomentaria fucoidan(SLF)in alleviating alcohol-induced liver injury was evaluated in a mouse model.It showed that SLF increased body weight and colon length,while reducing liver index,serum lipid,alanine aminotransferase,and aspartate aminotransferase in alcohol-treated mice.SLF inhibited inflammatory response in the liver by reducing inflammatory infiltration and the levels of pro-inflammatory cytokines.It can be associated with the alleviation of oxidative stress and the inhibition of the nuclear factor-κB pathway.SLF modulated alcohol-induced dysbiosis of gut microbiota,including a reduction in Bacteroidetes and Proteobacteria,and improved metabolites profile,primarily affecting short chain fatty acids and amino acids metabolism.In addition,SLF reduced the level of total bile acids,regulated the profile of bile acids,and increased the levels of farnesoid X receptor(FXR)and AMP-activated protein kinase(AMPK),suggesting that SLF can alleviate alcohol-induced liver injury by regulating bile acid-FXR/AMPK pathway.This study suggests that SLF holds the potential to alleviate the adverse effect of alcohol on the liver via the gut-liver axis.
基金supported by the National Natural Science Foundation of China(No.31901617)the National Key Research and Development Program of China(2017YFD0400600)+8 种基金the Top Talent Project of Department of Education of Anhui Province(gxyqZD2021125)the Excellent Talent project of Anhui Science and Technology University(XJYXRC202101)the Stable Talent Personnel Project of Anhui Science and Technology University(SPWD202101)the Natural Science Research Foundation of Department of Education of Anhui Province(2022AH040236)Project of Science and Technology Innovation Team of Anhui Science and Technology University(2023KJCXTD003)the Undergraduate innovation and entrepreneurship training program of Anhui Province(202410879088)the Excellent Research and Innovation Team of Anhui Province(2024AH010007)Discipline Leader Development Program of Anhui Province(DTR2024034)Anhui Province Youth Top Talent Scholar Program.
文摘The 3,5-dicaffeoylquinic acid(3,5-DiCQA),a key component of Chuzhou chrysanthemum,one of the“Four Major Edible Chrysanthemums”in China,has been shown to enrich beneficial gut bacteria and reduce lipid synthesis based on its hydroxycinnamic acid structures.However,its low lipophilicity limits bioactivity,necessitating the development of more stable derivatives.Therefore,this study synthesized and characterized a novel esterified derivative 3,5-DiCQA dodecyl ester(3,5-DiCQAD)with markedly enhanced lipophilicity and bioactivity.In highfat diet(HFD)-induced male C57BL/6J mice,3,5-DiCQAD showed remarkable metabolic regulatory effects,including improvements in glucose metabolism,lipid homeostasis,BA signaling,and thermogenesis.Furthermore,3,5-DiCQAD effectively reshaped the gut microbiota,enriching beneficial bacteria such as Lactobacillus plantarum.Moreover,the strain L.plantarum ZZ 240321 was isolated,and its fermentation of 3,5-DiCQAD generated metabolites that modulated bile acid(BA)metabolism through the FXR/SHP/LRH-1 pathway,alleviating obesity-related symptoms in antibiotic-treated mice.This research provides a novel perspective for developing plant-derived polyphenols as anti-obesity treatments.It also broadens the understanding of DiCQA’s probiotic potential in the gut microbiome,highlighting its promising role in managing metabolism-associated disorders.
基金supported by the National Natural Science Foundation of China(No.31901617)the National Key Research and Development Program of China(2017YFD0400600)+8 种基金the Top Talent Project of Department of Education of Anhui Province(gxyqZD2021125)the Excellent Talent project of Anhui Science and Technology University(XJYXRC202101)the Stable Talent Personnel Project of Anhui Science and Technology University(SPWD202101)the Natural Science Research Foundation of Department of Education of Anhui Province(2022AH040236)Project of Science and Technology Innovation Team of Anhui Science and Technology University(2023KJCXTD003)the Undergraduate Innovation and Entrepreneurship Training Program of Anhui Province(S202310879232)the Excellent Research and Innovation Team of Anhui Province Universities(2024AH010007)Discipline Leader Development Program of Anhui Province(DTR2024034)Anhui Province Youth Top Talent Scholar Program.
文摘Shuiman tea,a unique variant of Pu-erh tea,contains polysaccharides known for their antioxidant and hypoglycemic activities.However,their anti-obesity potential remains unexplored.In this study,a homogeneous acidic polysaccharide,STPS-3-1,was successfully isolated from Shuiman tea,and its preliminary structure and digestion characteristics were comprehensively analyzed.After 12 weeks of STPS-3-1 intervention,high-fat diet(HFD)-induced male C57BL/6J mice(specific pathogen free(SPF)grade,6 weeks old,18–20 g)exhibited significant metabolic improvements,including improved blood glucose and lipid levels,mitigated metabolic disorders,and reduced inflammation.Furthermore,STPS-3-1 notably reshaped the gut microbiota,enriching key species such as Bacteroides vulgatus.Subsequently,the B.vulgatus LWZ 240601 strain was successfully isolated and identified.This strain efficiently degraded STPS-3-1 under the synergistic action of three polysaccharide utilization loci(PULs),producing metabolites that regulated bile acid(BA)metabolism through the FXR/SHP signaling pathway,significantly alleviating obesity-related symptoms in antibiotic(ABX)-treated mice.This study provides valuable insights into the metabolic regulatory mechanisms and anti-obesity potential of STPS-3-1,elucidating their probiotic effects through gut microbiota modulation.Additionally,it expands the potential of tea polysaccharides in gut microbiota modulation and metabolic disease intervention,supporting the development of plant-based functional foods.
