Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disorder characterized by the degeneration of motor neurons in the brain and spinal cord,leading to muscle weakness,paralysis,and ultimately death(C...Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disorder characterized by the degeneration of motor neurons in the brain and spinal cord,leading to muscle weakness,paralysis,and ultimately death(Cleveland and Rothstein,2001).Frontotemporal lobar degeneration(FTLD)is a neurodegenerative disease affecting the frontal and temporal lobes of the brain,leading to changes in behavior,personality,and language(Van Langenhove et al.,2012).Both ALS and FTLD are classified as proteinopathies in which abnormal protein aggregation and accumulation in neurons contribute to the disease pathogenesis.Fused in sarcoma(FUS)is a DNA/RNA-binding protein involved in various cellular processes,including transcriptional regulation,RNA splicing,and DNA repair.Mutations in the FUS gene have been linked to familial ALS,highlighting the importance of FUS in the disease pathogenesis(Vance et al.,2009).In ALS and FTLD,aberrant post-translational modifications(PTMs)of FUS,such as phosphorylation,acetylation,and methylation,have been implicated in the promotion of FUS aggregation and neurotoxicity(Choi et al.,2023).Therefore,understanding the regulatory mechanisms of FUS PTMs is crucial for developing targeted therapies against these diseases.展开更多
At present, multi-se nsor fusion is widely used in object recognition and classification, since this technique can efficiently improve the accuracy and the ability of fault toleranc e. This paper describes a multi-se...At present, multi-se nsor fusion is widely used in object recognition and classification, since this technique can efficiently improve the accuracy and the ability of fault toleranc e. This paper describes a multi-sensor fusion system, which is model-based and used for rotating mechanical failure diagnosis. In the data fusion process, the fuzzy neural network is selected and used for the data fusion at report level. By comparing the experimental results of fault diagnoses based on fusion data wi th that on original separate data,it is shown that the former is more accurate than the latter.展开更多
A data fusion method of online multisensors is prop os ed in this paper based on artificial neuron. First, the dynamic data fusion mode l on artificial neuron is built. Then the calibration of data fusion is discusse ...A data fusion method of online multisensors is prop os ed in this paper based on artificial neuron. First, the dynamic data fusion mode l on artificial neuron is built. Then the calibration of data fusion is discusse d with self-adaptive weighing technique. Finally performance of the method is d emonstrated by an online vibration measurement case. The results show that the f used data are more stable, sensitive, accurate, reliable than that of single sen sor data.展开更多
Liquid-liquid phase separation(LLPS)or biomolecular condensation that leads to formation of membraneless organelles plays a critical role in many biochemical processes.Mechanism study of regulating LLPS is therefore c...Liquid-liquid phase separation(LLPS)or biomolecular condensation that leads to formation of membraneless organelles plays a critical role in many biochemical processes.Mechanism study of regulating LLPS is therefore central to the understanding of protein aggregation and disease-relevant process.We report a fused in sarcoma protein(FUS)-derived low complexity(LC)sequence that undergoes LLPS in the presence of metal ions.The LC protein was constructed by fusing a hexhistidine-tag to the N-terminal low complexity domain(the residues 1–165 in QGSY-rich segment)of FUS.Spontaneous condensation of the intrinsic disordered protein into coacervate droplets was observed in the presence of metal ions that chelate oligohistidine moieties to form protein matrix.We demonstrate the key role of metal ion-histidine coordination in governing LLPS behaviours and the fluidity of biomolecular condensates.By taking advantage of competitive binding using chelators,we show the possibility of regulating dynamic behaviors of disease-relevant protein droplets,and developing a potential approach towards controllable biological encapsulation/release.展开更多
In this study,six kinetics models of indomethacin hydrophilic gel patch transdermal in vitro release was established,including zero-level,first-order,Higuchi-level,Ritger-Peppas,Weibull and Hixcon-Crowell dynamic equa...In this study,six kinetics models of indomethacin hydrophilic gel patch transdermal in vitro release was established,including zero-level,first-order,Higuchi-level,Ritger-Peppas,Weibull and Hixcon-Crowell dynamic equations.The chemical permeation enhancers,including 3%and 5%Azone,and iontophoresis were used as the control.Transdermal diffusion tests were performed in vitro and indomethacin was quantified by high performance liquid chromatography system.The transdermal parameter of the Higuchi and Weibull dynamic equations,indicated that Fu’s cupping therapy(FCT)could significantly improve Higuchi and Weibull kinetic parameters in vitro transdermal,increased transdermal rate and permeability coefficient,reduced lagging time.Additionally,statistical analysis speculated the skin barrier function could be restored after 46 h treatment.Hence,as a new physical transdermal drug delivery technology,transdermal permeation effects produced by FCT are obvious,which has the characteristics of traditional Chinese medicine and has important clinical application value.展开更多
基金supported by the BK21 FOUR(Fostering Outstanding Universities for Research)and the Basic Science Research Program through the National Research Foundation of Korea(NRF),funded by the Ministry of Education(MOE)and the Ministry of Science and ICT(MSIT)(NRF-2022R1A2C1004204,RS-2023-00219563,2023-DD-UP-0007)by the Soonchunhyang University Research Fund(to KK)。
文摘Amyotrophic lateral sclerosis(ALS)is a progressive neurodegenerative disorder characterized by the degeneration of motor neurons in the brain and spinal cord,leading to muscle weakness,paralysis,and ultimately death(Cleveland and Rothstein,2001).Frontotemporal lobar degeneration(FTLD)is a neurodegenerative disease affecting the frontal and temporal lobes of the brain,leading to changes in behavior,personality,and language(Van Langenhove et al.,2012).Both ALS and FTLD are classified as proteinopathies in which abnormal protein aggregation and accumulation in neurons contribute to the disease pathogenesis.Fused in sarcoma(FUS)is a DNA/RNA-binding protein involved in various cellular processes,including transcriptional regulation,RNA splicing,and DNA repair.Mutations in the FUS gene have been linked to familial ALS,highlighting the importance of FUS in the disease pathogenesis(Vance et al.,2009).In ALS and FTLD,aberrant post-translational modifications(PTMs)of FUS,such as phosphorylation,acetylation,and methylation,have been implicated in the promotion of FUS aggregation and neurotoxicity(Choi et al.,2023).Therefore,understanding the regulatory mechanisms of FUS PTMs is crucial for developing targeted therapies against these diseases.
文摘At present, multi-se nsor fusion is widely used in object recognition and classification, since this technique can efficiently improve the accuracy and the ability of fault toleranc e. This paper describes a multi-sensor fusion system, which is model-based and used for rotating mechanical failure diagnosis. In the data fusion process, the fuzzy neural network is selected and used for the data fusion at report level. By comparing the experimental results of fault diagnoses based on fusion data wi th that on original separate data,it is shown that the former is more accurate than the latter.
文摘A data fusion method of online multisensors is prop os ed in this paper based on artificial neuron. First, the dynamic data fusion mode l on artificial neuron is built. Then the calibration of data fusion is discusse d with self-adaptive weighing technique. Finally performance of the method is d emonstrated by an online vibration measurement case. The results show that the f used data are more stable, sensitive, accurate, reliable than that of single sen sor data.
基金financially supported by the National Natural Science Foundation of China (Nos. 22072159 and 22172007)the Fundamental Research Funds for the Central Universities(No. buctrc202015)
文摘Liquid-liquid phase separation(LLPS)or biomolecular condensation that leads to formation of membraneless organelles plays a critical role in many biochemical processes.Mechanism study of regulating LLPS is therefore central to the understanding of protein aggregation and disease-relevant process.We report a fused in sarcoma protein(FUS)-derived low complexity(LC)sequence that undergoes LLPS in the presence of metal ions.The LC protein was constructed by fusing a hexhistidine-tag to the N-terminal low complexity domain(the residues 1–165 in QGSY-rich segment)of FUS.Spontaneous condensation of the intrinsic disordered protein into coacervate droplets was observed in the presence of metal ions that chelate oligohistidine moieties to form protein matrix.We demonstrate the key role of metal ion-histidine coordination in governing LLPS behaviours and the fluidity of biomolecular condensates.By taking advantage of competitive binding using chelators,we show the possibility of regulating dynamic behaviors of disease-relevant protein droplets,and developing a potential approach towards controllable biological encapsulation/release.
基金This work was supported by the Projects[NO.20154030 and NO.(2017)5655]from the Science and Technology Department of Guizhou Province and the National Natural Science Foundation of China(No.81873020).
文摘In this study,six kinetics models of indomethacin hydrophilic gel patch transdermal in vitro release was established,including zero-level,first-order,Higuchi-level,Ritger-Peppas,Weibull and Hixcon-Crowell dynamic equations.The chemical permeation enhancers,including 3%and 5%Azone,and iontophoresis were used as the control.Transdermal diffusion tests were performed in vitro and indomethacin was quantified by high performance liquid chromatography system.The transdermal parameter of the Higuchi and Weibull dynamic equations,indicated that Fu’s cupping therapy(FCT)could significantly improve Higuchi and Weibull kinetic parameters in vitro transdermal,increased transdermal rate and permeability coefficient,reduced lagging time.Additionally,statistical analysis speculated the skin barrier function could be restored after 46 h treatment.Hence,as a new physical transdermal drug delivery technology,transdermal permeation effects produced by FCT are obvious,which has the characteristics of traditional Chinese medicine and has important clinical application value.
