Objective: To investigate biomolecular alteration of sesamol on human lung adenocarcinoma(SK-LU-1) cells compared with cisplatin using Fourier transform infrared microscopy(FTIR). Methods: Cytotoxicity of sesamol was ...Objective: To investigate biomolecular alteration of sesamol on human lung adenocarcinoma(SK-LU-1) cells compared with cisplatin using Fourier transform infrared microscopy(FTIR). Methods: Cytotoxicity of sesamol was investigated against SK-LU-1 cells by using neutral red. DNA fragmentation and the cell cycle analysis were determined by agarose gel electrophoresis and flow cytometry, respectively. The FTIR microscopy technique was applied to explore the changes in cellular biochemical compositions in cells treated with sesamol that the biochemical and biological assays cannot cover. The alkylating property was determined by 4-(4-nitrobenzyl)pyridine assay. Results: Sesamol and cisplatin exerted an antiproliferative effect at 48 h with respective IC50 values of 2.7 and 0.07 m M. Both induced apoptosis by causing DNA damage and accumulation of cell populations at sub-G1. FTIR microscopy and Principle Component Analysis clearly discriminated the sesamol-and cisplatin-treated cells from the untreated cells or control. A significant increase of total lipid content was found in cisplatin-treated cells. Conformational changes in the proteins secondary structure from the β-helix to the β-sheet were found in both sesamol-and cisplatin-treated cells, as well as significant reductions in relative DNA content of both compared to the control were observed, suggesting DNA damage. A shift in the peak position of DNA region provides insight on the DNA interactions. Conclusions: The non-alkylating effect of sesamol based on the nitrobenzyl pyridine assay delineates the non-covalent binding mode of sesamol on DNA. Hydrogen bonding is the binding mode of sesamol on DNA, while for cisplatin it was covalent and hydrogen bonding.展开更多
Ce^（3＋） substituted Cu-spinel nanoferrites CuCe_xFe_（2-x）O_4（x=0.00, 0.02, 0.04, 0.06, 0.08 and 0.10） were synthesized via sol-gel self-combustion hybrid route. Single phase spinel ferrite of Cu nanoferrites we...Ce^（3＋） substituted Cu-spinel nanoferrites CuCe_xFe_（2-x）O_4（x=0.00, 0.02, 0.04, 0.06, 0.08 and 0.10） were synthesized via sol-gel self-combustion hybrid route. Single phase spinel ferrite of Cu nanoferrites were examined using X-ray diffraction（XRD） analysis whereas the multiphase structure was observed as Ce contents increased from x=0.06. Field emission scanning electron microscopy（FESEM）, Thermogravimetric and differential thermal analysis（TGA and DTA） and Fourier transform infrared spectroscopy（FTIR） were used to find out the morphology phase and metal stretching vibrations of Ce^（3＋） substituted nanocrystalline ferrites. The crystallite size was increased and found in the range of 25-91 nm. The agglomerations in Cu ferrite samples increase as the Ce^（3＋） concentration increases. The magnetic properties such as remanence, saturation magnetization, coercivity, Bohr magneton and magnetocrystalline anisotropy constant（K） were determined using M-H loops recorded from a vibrating sample magnetometer（VSM）. Saturation magnetization, remanence and coercivity are increased as the Ce^（3＋）contents increase in Cu nanocrystalline samples. Moreover, law of approach to saturation（LoA） was used to calculate the maximum value of saturation for Ce-doped Cu nanoferrites. The soft magnetic behaviour of the Cu nanoferrite is observed as compared to the samples substituted with the increased Ce contents in Cu nanocrystalline ferrite. Bohr magneton and magnetocrystalline anisotropy are found to increase with the substitution of rare earth Ce^（3＋） contents in Cu spinel nanocrystalline ferrite. Cedoped Cu nanocrystalline ferrites with excellent properties may be suitable for potential applications in sensing, security, switching, core, multilayer chip inductor, biomedical and microwave absorption applications.展开更多
The study investigates the magnetic separation of Fe from automobile shredder residue （ASR） （ 〈 0.25 mm） and its application for phenol degradation in water. The magnetically separated Fe was subjected to an ultr...The study investigates the magnetic separation of Fe from automobile shredder residue （ASR） （ 〈 0.25 mm） and its application for phenol degradation in water. The magnetically separated Fe was subjected to an ultrasonically assisted acid treatment, and the degradation of phenol in an aqueous solution using nano/micro-size Fe （n/m Fe） was investigated in an effort to evaluate the possibility of utilizing n/m Fe to remove phenol from wastewater. The prepared n/m Fe was analyzed by scanning electron microscopy （SEM） and Fourier transform infrared spectroscopy （FTIR）. The eflects of the dosages ot n/mFe, pH, concentration of phenol and amount of H2O2 on phenol removal were evaluated. The results confirm that the phenol degradation rate was improved with an increase in the dosages of n/mFe and H2O2; however, the rate is reduced when the phenol concentration is higher. The degradation of phenol by n/mFe followed the pseudo-first-order kinetics. The value of the reaction rate constant （k）was increased as the amounts of n/m Fe and H2O2 increased. Conversely, the value of k was reduced when the concentration of phenol was increased. The probable mechanism behind the degradation of phenol by n/m Fe is the oxidation of phenol through hydroxyl radicals which are produced during the reaction between H2O2 and n/m Fe.展开更多
基金supported by the Higher Education Research Promotion and National Research University Project of Thailand,Office of the Higher Education Commission,through the Food and Functional Food Research Cluster and Research and Development of Herbal Nutraceutics Subcluster of Khon Kaen University(FC 3.1.13 PhD and NRU 541057)
文摘Objective: To investigate biomolecular alteration of sesamol on human lung adenocarcinoma(SK-LU-1) cells compared with cisplatin using Fourier transform infrared microscopy(FTIR). Methods: Cytotoxicity of sesamol was investigated against SK-LU-1 cells by using neutral red. DNA fragmentation and the cell cycle analysis were determined by agarose gel electrophoresis and flow cytometry, respectively. The FTIR microscopy technique was applied to explore the changes in cellular biochemical compositions in cells treated with sesamol that the biochemical and biological assays cannot cover. The alkylating property was determined by 4-(4-nitrobenzyl)pyridine assay. Results: Sesamol and cisplatin exerted an antiproliferative effect at 48 h with respective IC50 values of 2.7 and 0.07 m M. Both induced apoptosis by causing DNA damage and accumulation of cell populations at sub-G1. FTIR microscopy and Principle Component Analysis clearly discriminated the sesamol-and cisplatin-treated cells from the untreated cells or control. A significant increase of total lipid content was found in cisplatin-treated cells. Conformational changes in the proteins secondary structure from the β-helix to the β-sheet were found in both sesamol-and cisplatin-treated cells, as well as significant reductions in relative DNA content of both compared to the control were observed, suggesting DNA damage. A shift in the peak position of DNA region provides insight on the DNA interactions. Conclusions: The non-alkylating effect of sesamol based on the nitrobenzyl pyridine assay delineates the non-covalent binding mode of sesamol on DNA. Hydrogen bonding is the binding mode of sesamol on DNA, while for cisplatin it was covalent and hydrogen bonding.
文摘Ce^（3＋） substituted Cu-spinel nanoferrites CuCe_xFe_（2-x）O_4（x=0.00, 0.02, 0.04, 0.06, 0.08 and 0.10） were synthesized via sol-gel self-combustion hybrid route. Single phase spinel ferrite of Cu nanoferrites were examined using X-ray diffraction（XRD） analysis whereas the multiphase structure was observed as Ce contents increased from x=0.06. Field emission scanning electron microscopy（FESEM）, Thermogravimetric and differential thermal analysis（TGA and DTA） and Fourier transform infrared spectroscopy（FTIR） were used to find out the morphology phase and metal stretching vibrations of Ce^（3＋） substituted nanocrystalline ferrites. The crystallite size was increased and found in the range of 25-91 nm. The agglomerations in Cu ferrite samples increase as the Ce^（3＋） concentration increases. The magnetic properties such as remanence, saturation magnetization, coercivity, Bohr magneton and magnetocrystalline anisotropy constant（K） were determined using M-H loops recorded from a vibrating sample magnetometer（VSM）. Saturation magnetization, remanence and coercivity are increased as the Ce^（3＋）contents increase in Cu nanocrystalline samples. Moreover, law of approach to saturation（LoA） was used to calculate the maximum value of saturation for Ce-doped Cu nanoferrites. The soft magnetic behaviour of the Cu nanoferrite is observed as compared to the samples substituted with the increased Ce contents in Cu nanocrystalline ferrite. Bohr magneton and magnetocrystalline anisotropy are found to increase with the substitution of rare earth Ce^（3＋） contents in Cu spinel nanocrystalline ferrite. Cedoped Cu nanocrystalline ferrites with excellent properties may be suitable for potential applications in sensing, security, switching, core, multilayer chip inductor, biomedical and microwave absorption applications.
文摘The study investigates the magnetic separation of Fe from automobile shredder residue （ASR） （ 〈 0.25 mm） and its application for phenol degradation in water. The magnetically separated Fe was subjected to an ultrasonically assisted acid treatment, and the degradation of phenol in an aqueous solution using nano/micro-size Fe （n/m Fe） was investigated in an effort to evaluate the possibility of utilizing n/m Fe to remove phenol from wastewater. The prepared n/m Fe was analyzed by scanning electron microscopy （SEM） and Fourier transform infrared spectroscopy （FTIR）. The eflects of the dosages ot n/mFe, pH, concentration of phenol and amount of H2O2 on phenol removal were evaluated. The results confirm that the phenol degradation rate was improved with an increase in the dosages of n/mFe and H2O2; however, the rate is reduced when the phenol concentration is higher. The degradation of phenol by n/mFe followed the pseudo-first-order kinetics. The value of the reaction rate constant （k）was increased as the amounts of n/m Fe and H2O2 increased. Conversely, the value of k was reduced when the concentration of phenol was increased. The probable mechanism behind the degradation of phenol by n/m Fe is the oxidation of phenol through hydroxyl radicals which are produced during the reaction between H2O2 and n/m Fe.
