The detailed glycan structural analysis of glycoprotein is amenable to glycopeptide enrichment. Here, we develop a simple, effective and economical approach to enrich glycopeptides from proteolytically digested peptid...The detailed glycan structural analysis of glycoprotein is amenable to glycopeptide enrichment. Here, we develop a simple, effective and economical approach to enrich glycopeptides from proteolytically digested peptide mixtures by chromatographic column packed with graphite carbon and activated charcoal (G/A-column). Glycopeptide from ovalbumin was efficiently enriched by homemade G/A-column using liquid chromatography and the structure of glycopeptide was obtained by tandem mass spectrometry using Fourier transform ion cyclotron resonance mass spectrometry. The results in this study demonstrate that G/A-column can be used to enrich N-glycolpeptides and be benefit for online identification of glycopeptide using LC-MS.展开更多
湖泊生态系统中溶解性有机物(dissolved organic matter,DOM)来源复杂,不同污染源输入差异显著,并深刻影响着湖泊物质循环与生态功能。以洞庭湖为研究对象,利用傅里叶变换离子回旋共振质谱(Fourier transform ion cyclotron resonance m...湖泊生态系统中溶解性有机物(dissolved organic matter,DOM)来源复杂,不同污染源输入差异显著,并深刻影响着湖泊物质循环与生态功能。以洞庭湖为研究对象,利用傅里叶变换离子回旋共振质谱(Fourier transform ion cyclotron resonance mass spectrometry,FT-ICR MS)分子表征技术,结合主成分分析(principal component analysis,PCA)-绝对主成分分数(absolute principal component scores,APCS)-多元线性回归(multiple linear regression,MLR)受体模型,定量解析湖区外源DOM的分子特征及贡献。结果表明:湖水DOM以CHO化合物为主,枯水期富含含硫化合物,丰水期含氮化合物比例较高;2个季节的DOM均以高度不饱和类化合物为主,且丰水期DOM的芳香性和稳定性更强;污染源DOM整体不饱和度和芳香性较高,难以降解;受体模型定量结果显示,外源对DOM的贡献顺序为农田水(37.7%)>污水(26.7%)>鱼塘水(18.3%)>未知来源(17.3%)。研究揭示了农业面源污染和生活污水是洞庭湖DOM的主要输入来源,可为湖泊DOM迁移转化机制解析和流域污染治理提供科学依据。展开更多
For many years, HUVEC.com1 public database provides biological data relative to the proteome of human umbilical vein endothelial cells (HU-VECs), which are the most used human endothelial cell model in vascular biolog...For many years, HUVEC.com1 public database provides biological data relative to the proteome of human umbilical vein endothelial cells (HU-VECs), which are the most used human endothelial cell model in vascular biology. The proteins were identified using two-dimensional gel electrophoresis (2-DGE) for protein separation coupled with Matrix Assisted Laser Desorption-Ionization Mass Spectrometry (MALDI-TOF-MS) for identification. We present here an important update of HUVEC.com with 521 protein identifications as determined using Fourier transformed ion cyclotron resonance-mass spectrometry (FTICR-MS) applied to an unstained 2-DGE gel cut in 221 squared pieces;each identified protein being accompanied by a semi-quantitative three dimensional visualization is called “score imaging”. The squared analyzed gel and the alphabetical list of identified proteins, linked with their corresponding three-dimensional score imaging, are available at www.huvec.com. This original approach led to the establishment of the most protein-rich and informative database for HUVECs, as well as to the identification of some protein species, in particular with phosphorylation.展开更多
文摘The detailed glycan structural analysis of glycoprotein is amenable to glycopeptide enrichment. Here, we develop a simple, effective and economical approach to enrich glycopeptides from proteolytically digested peptide mixtures by chromatographic column packed with graphite carbon and activated charcoal (G/A-column). Glycopeptide from ovalbumin was efficiently enriched by homemade G/A-column using liquid chromatography and the structure of glycopeptide was obtained by tandem mass spectrometry using Fourier transform ion cyclotron resonance mass spectrometry. The results in this study demonstrate that G/A-column can be used to enrich N-glycolpeptides and be benefit for online identification of glycopeptide using LC-MS.
文摘湖泊生态系统中溶解性有机物(dissolved organic matter,DOM)来源复杂,不同污染源输入差异显著,并深刻影响着湖泊物质循环与生态功能。以洞庭湖为研究对象,利用傅里叶变换离子回旋共振质谱(Fourier transform ion cyclotron resonance mass spectrometry,FT-ICR MS)分子表征技术,结合主成分分析(principal component analysis,PCA)-绝对主成分分数(absolute principal component scores,APCS)-多元线性回归(multiple linear regression,MLR)受体模型,定量解析湖区外源DOM的分子特征及贡献。结果表明:湖水DOM以CHO化合物为主,枯水期富含含硫化合物,丰水期含氮化合物比例较高;2个季节的DOM均以高度不饱和类化合物为主,且丰水期DOM的芳香性和稳定性更强;污染源DOM整体不饱和度和芳香性较高,难以降解;受体模型定量结果显示,外源对DOM的贡献顺序为农田水(37.7%)>污水(26.7%)>鱼塘水(18.3%)>未知来源(17.3%)。研究揭示了农业面源污染和生活污水是洞庭湖DOM的主要输入来源,可为湖泊DOM迁移转化机制解析和流域污染治理提供科学依据。
基金Financial support from the TGE FT-ICR for conducting the research is gratefully acknowledged.
文摘For many years, HUVEC.com1 public database provides biological data relative to the proteome of human umbilical vein endothelial cells (HU-VECs), which are the most used human endothelial cell model in vascular biology. The proteins were identified using two-dimensional gel electrophoresis (2-DGE) for protein separation coupled with Matrix Assisted Laser Desorption-Ionization Mass Spectrometry (MALDI-TOF-MS) for identification. We present here an important update of HUVEC.com with 521 protein identifications as determined using Fourier transformed ion cyclotron resonance-mass spectrometry (FTICR-MS) applied to an unstained 2-DGE gel cut in 221 squared pieces;each identified protein being accompanied by a semi-quantitative three dimensional visualization is called “score imaging”. The squared analyzed gel and the alphabetical list of identified proteins, linked with their corresponding three-dimensional score imaging, are available at www.huvec.com. This original approach led to the establishment of the most protein-rich and informative database for HUVECs, as well as to the identification of some protein species, in particular with phosphorylation.
