[Objective] This study aimed to establish a determination method for iprobenfos residue in rice straw and husked rice. [Method] The rice straw and husked rice samples were extracted by acetone-ethyl acetate mixed solv...[Objective] This study aimed to establish a determination method for iprobenfos residue in rice straw and husked rice. [Method] The rice straw and husked rice samples were extracted by acetone-ethyl acetate mixed solvent. The extracts were purified using SPE C18 column and SPE NH2 column, and the iprobenfos residues were determined by GC-FTD. [Result] In the concentration range of 0.005-5.0 mg/kg, iprobenfos concentration showed a good linear relationship with peak area （r=0.999 8）. When the iprobenfos concentrations were 0.01, 0.1 and 1.0 mg/kg respectively, the recoveries of added iprobenfos from rice straw ranged from 72.6% to 99.7% with relative standard deviation ranging from 5.65% to 8.48%; the recoveries of added iprobenfos from husked rice ranged from 81.6% to 97.6% with relative standard deviation ranging from 3.74% to 7.63%. The minimum detectable quantity of iprobenfos was 5×10^-12 g, and the minimum detectable concentrations of iprobenfos in rice straw and husked rice samples were 2.0 and 0.5 μg/kg, respec- tively. [Conclusion] The established determination method is characterized by low de- termination limit, high sensitivity, good reproducibility and high operability, which all meet the requirements by Guideline on Pesticide Residue Trials of the Ministry of Agriculture.展开更多
Today, the growth of aerospace industry has led to the development of modular overhead systems that can be applied to a wide variety of aircraft.?It actually maximizes training effectiveness by working in a similar wa...Today, the growth of aerospace industry has led to the development of modular overhead systems that can be applied to a wide variety of aircraft.?It actually maximizes training effectiveness by working in a similar way as to the real aircraft maneuverability. Overhead system control device for aircraft simulator is developed as a module and integrated to the flight simulator. The developed system can replace the similar products imported from overseas at a much lower price, about one third of the imports, while maintaining the same level of functionality and the performance with the counterparts. This price advantage is the main motivation of this development, which is expected to enlarge the commercial training simulator market in our country. This development has been also funded by the government, and we invited several commercial airline pilots to test the equipment. The post operation interview revealed that the developed system at least matches or exceeds the performance of the imported products. With the development completed, it is ready for the commercial production and will help promote the expansion of flight training education at various aerospace universities in Korea.展开更多
The “ applause sign” is a simple test of motor control that helps to diffe rentiate PSP from frontal or striatofrontal degenerative diseases. It was found in 0/39 controls, 0 of 24 patients with frontotemporal demen...The “ applause sign” is a simple test of motor control that helps to diffe rentiate PSP from frontal or striatofrontal degenerative diseases. It was found in 0/39 controls, 0 of 24 patients with frontotemporal dementia (FTD), 0 of 17 p atients with Parkinson disease (PD), and 30/42 patients with progressive supranu clear palsy (PSP). It discriminated PSP from FTD (p < 0.001) and PD (p < 0.00). The “ three clap test” correctly identified 81.8% of the patients in the co mparison PSP and FTD and 75% of the patients in the comparison of PSP and PD.展开更多
A largely increased number of GGGGCC repeats located in the non-coding region of C9orf72 gene have been identified as the leading cause of two related neurological disorders, familial amyotrophic lateral sclero- sis ...A largely increased number of GGGGCC repeats located in the non-coding region of C9orf72 gene have been identified as the leading cause of two related neurological disorders, familial amyotrophic lateral sclero- sis （ALS） and frontotemporal dementia （FTD）. We examined G-quadruplex forming ability of GGGGCC- repeat containing oligonucleotides with four guanine tracts chosen as the smallest possible model to form a unimolecular G-quadruplex. These oligonucleotides are readily to folded into G-quadruplexes in the presence of K~ ions. However, the formation of multiple structures makes structural analysis challenging and time consuming. We observed that flanking sequences on 5＇- and 3＇-ends as well as mutations of loop residues have a profound effect on folding. Sequence d[（GaC2）3G4] was chosen for further scrutiny and optimization of nuclear magnetic resonance （NMR） spectroscopic properties with dG to 8Br-dG substitutions at specific positions in the sequence under different folding conditions. Expectedly, folding into desired predominant topology is facilitated when sub- stituted residue adopted a syn conformation in the naturally-occurring structure. Single dG to 8Br-dG sub- stitution at position 21 and fine tuning of folding conditions facilitate folding of d[（G4C2）3GG^BrGG] into （mostly） a single G-quadruplex, and thus enable determi- nation of its high-resolution structure by high-field NMR.展开更多
基金Supported by Pesticide Registration Residual Test of Institute for the Control of Agrochemicals,Ministry of Agriculture(2013F216)~~
文摘[Objective] This study aimed to establish a determination method for iprobenfos residue in rice straw and husked rice. [Method] The rice straw and husked rice samples were extracted by acetone-ethyl acetate mixed solvent. The extracts were purified using SPE C18 column and SPE NH2 column, and the iprobenfos residues were determined by GC-FTD. [Result] In the concentration range of 0.005-5.0 mg/kg, iprobenfos concentration showed a good linear relationship with peak area （r=0.999 8）. When the iprobenfos concentrations were 0.01, 0.1 and 1.0 mg/kg respectively, the recoveries of added iprobenfos from rice straw ranged from 72.6% to 99.7% with relative standard deviation ranging from 5.65% to 8.48%; the recoveries of added iprobenfos from husked rice ranged from 81.6% to 97.6% with relative standard deviation ranging from 3.74% to 7.63%. The minimum detectable quantity of iprobenfos was 5×10^-12 g, and the minimum detectable concentrations of iprobenfos in rice straw and husked rice samples were 2.0 and 0.5 μg/kg, respec- tively. [Conclusion] The established determination method is characterized by low de- termination limit, high sensitivity, good reproducibility and high operability, which all meet the requirements by Guideline on Pesticide Residue Trials of the Ministry of Agriculture.
文摘Today, the growth of aerospace industry has led to the development of modular overhead systems that can be applied to a wide variety of aircraft.?It actually maximizes training effectiveness by working in a similar way as to the real aircraft maneuverability. Overhead system control device for aircraft simulator is developed as a module and integrated to the flight simulator. The developed system can replace the similar products imported from overseas at a much lower price, about one third of the imports, while maintaining the same level of functionality and the performance with the counterparts. This price advantage is the main motivation of this development, which is expected to enlarge the commercial training simulator market in our country. This development has been also funded by the government, and we invited several commercial airline pilots to test the equipment. The post operation interview revealed that the developed system at least matches or exceeds the performance of the imported products. With the development completed, it is ready for the commercial production and will help promote the expansion of flight training education at various aerospace universities in Korea.
文摘The “ applause sign” is a simple test of motor control that helps to diffe rentiate PSP from frontal or striatofrontal degenerative diseases. It was found in 0/39 controls, 0 of 24 patients with frontotemporal dementia (FTD), 0 of 17 p atients with Parkinson disease (PD), and 30/42 patients with progressive supranu clear palsy (PSP). It discriminated PSP from FTD (p < 0.001) and PD (p < 0.00). The “ three clap test” correctly identified 81.8% of the patients in the co mparison PSP and FTD and 75% of the patients in the comparison of PSP and PD.
文摘A largely increased number of GGGGCC repeats located in the non-coding region of C9orf72 gene have been identified as the leading cause of two related neurological disorders, familial amyotrophic lateral sclero- sis （ALS） and frontotemporal dementia （FTD）. We examined G-quadruplex forming ability of GGGGCC- repeat containing oligonucleotides with four guanine tracts chosen as the smallest possible model to form a unimolecular G-quadruplex. These oligonucleotides are readily to folded into G-quadruplexes in the presence of K~ ions. However, the formation of multiple structures makes structural analysis challenging and time consuming. We observed that flanking sequences on 5＇- and 3＇-ends as well as mutations of loop residues have a profound effect on folding. Sequence d[（GaC2）3G4] was chosen for further scrutiny and optimization of nuclear magnetic resonance （NMR） spectroscopic properties with dG to 8Br-dG substitutions at specific positions in the sequence under different folding conditions. Expectedly, folding into desired predominant topology is facilitated when sub- stituted residue adopted a syn conformation in the naturally-occurring structure. Single dG to 8Br-dG sub- stitution at position 21 and fine tuning of folding conditions facilitate folding of d[（G4C2）3GG^BrGG] into （mostly） a single G-quadruplex, and thus enable determi- nation of its high-resolution structure by high-field NMR.
