荧光漂白恢复(fluorescence recovery after photobleaching,FRAP)技术作为定量分析细胞质膜流动性的关键手段之一,在细胞膜动力学研究领域具有广泛的应用价值。囊泡运输(包括胞吞作用与胞吐作用)作为细胞内外物质交换的核心生物学过程...荧光漂白恢复(fluorescence recovery after photobleaching,FRAP)技术作为定量分析细胞质膜流动性的关键手段之一,在细胞膜动力学研究领域具有广泛的应用价值。囊泡运输(包括胞吞作用与胞吐作用)作为细胞内外物质交换的核心生物学过程,其运行机制高度依赖于质膜的流动性。基于科教融合的教学理念,在“细胞生物学实验”研究生课程中融入FRAP技术实验模块,其核心目标在于引导学生探究“囊泡运输”与“细胞质膜流动性”的内在相关性。该实验教学体系不仅充分凸显了FRAP技术在活细胞动态监测中的独特优势,更通过“理论知识–实验操作–数据分析”的完整训练体系,强化了学生对膜流动性与囊泡运输等基础理论知识的理解;并通过引导学生探究膜异质性及微聚体空间组织特性等细胞生物学前沿科学问题,拓展了其学术视野,为后续开展膜蛋白相关研究奠定了方法学基础。展开更多
The variation of membrane surface and lateral diffusion of membrane protein was studied after the interaction of laminin with its membrane receptor in mouse macrophages. A pattern of membrane surface which showed smal...The variation of membrane surface and lateral diffusion of membrane protein was studied after the interaction of laminin with its membrane receptor in mouse macrophages. A pattern of membrane surface which showed smaller and bigger peaks was obtained by scanning tunneling microscope(STM), looking like the domains of lipid groups and proteins in the model of fluid mosaic biomembrane. Some even more higher and wider peaks projected out from the membrane surface in STM image after the interacting of laminin with membrane receptor were, probably, the complexes of laminin and membrane receptor. Furthermore, the decreased lateral diffusion coefficient value (D) was obtained by fluorescence recovery after photobleaching (FRAP) after the laminin was reacted with membrane receptor. This phenomenon provides an evidence that the complexes of laminin and its membrane receptor were located on the membrane of macrophages. So we could consider that the laminin is combined with membrane receptor leading to the variation in the properties of membrane surface.展开更多
文摘荧光漂白恢复(fluorescence recovery after photobleaching,FRAP)技术作为定量分析细胞质膜流动性的关键手段之一,在细胞膜动力学研究领域具有广泛的应用价值。囊泡运输(包括胞吞作用与胞吐作用)作为细胞内外物质交换的核心生物学过程,其运行机制高度依赖于质膜的流动性。基于科教融合的教学理念,在“细胞生物学实验”研究生课程中融入FRAP技术实验模块,其核心目标在于引导学生探究“囊泡运输”与“细胞质膜流动性”的内在相关性。该实验教学体系不仅充分凸显了FRAP技术在活细胞动态监测中的独特优势,更通过“理论知识–实验操作–数据分析”的完整训练体系,强化了学生对膜流动性与囊泡运输等基础理论知识的理解;并通过引导学生探究膜异质性及微聚体空间组织特性等细胞生物学前沿科学问题,拓展了其学术视野,为后续开展膜蛋白相关研究奠定了方法学基础。
基金a great from the Nationa Natural Science Foundation,
文摘The variation of membrane surface and lateral diffusion of membrane protein was studied after the interaction of laminin with its membrane receptor in mouse macrophages. A pattern of membrane surface which showed smaller and bigger peaks was obtained by scanning tunneling microscope(STM), looking like the domains of lipid groups and proteins in the model of fluid mosaic biomembrane. Some even more higher and wider peaks projected out from the membrane surface in STM image after the interacting of laminin with membrane receptor were, probably, the complexes of laminin and membrane receptor. Furthermore, the decreased lateral diffusion coefficient value (D) was obtained by fluorescence recovery after photobleaching (FRAP) after the laminin was reacted with membrane receptor. This phenomenon provides an evidence that the complexes of laminin and its membrane receptor were located on the membrane of macrophages. So we could consider that the laminin is combined with membrane receptor leading to the variation in the properties of membrane surface.
