AIM: To study the effect of FR167653 on immunologica liver injury (ILI) in mice.METHODS: ILI was established by tail vein injection of 2.5 mg Bacillus Calmette-Guerin(BCG), and 10 d later with 10 mg lipopolysaccharide...AIM: To study the effect of FR167653 on immunologica liver injury (ILI) in mice.METHODS: ILI was established by tail vein injection of 2.5 mg Bacillus Calmette-Guerin(BCG), and 10 d later with 10 mg lipopolysaccharide (LPS) in 0.2 mL saline (BCG plus LPS). Alanine aminotransferase (ALT), aspartate aminotransferase (AST) in sera and malondialdehyde (MDA), glutathione peroxidase (GSHpx) contents in liver homogenates were assayed by spectrophotometry. The levels of tumor necrosis factor-~ (TNF-~) and nitric oxide (NO) levels in sera were determined using ELISA. Interleukin-1 (IL-1) produced by peritoneal macrophages was determined by the method of 3^H-infiltrated cell proliferation. The nuclear factor-kappa B (NF-xB) p65 in liver tissue was analyzed with reverse transcription polymerase chain reaction (RT-PCR). Liver samples collected were stained with hematoxylin and eosin.RFSULTS: FR167653 (50, 100, 150 mg/kg) could significantly decrease the serum transaminase (ALl', AS'I-) activity and MDA content in liver homogenate, and improve reduced GSHpx level of liver homogenate. Liver histopathological examinatdon showed FR167653 (100, 150 mg/kg) significantly reduced inflammatory cells infiltration and liver cells necrosis. FR167653 (50, 100, 150 mg/kg) significantly lowered TNF-α and NO levels in serum, and IL-1 produced by peritoneal macrophages. Moreover, expression of NF-xBmRNA in liver tissue of ILl induced by BCG plus LPS was significantly reduced by FR167653.CONCLUSION: All results showed that FR167653 had significant inhibitorv action an ILI in mice.展开更多
文摘AIM: To study the effect of FR167653 on immunologica liver injury (ILI) in mice.METHODS: ILI was established by tail vein injection of 2.5 mg Bacillus Calmette-Guerin(BCG), and 10 d later with 10 mg lipopolysaccharide (LPS) in 0.2 mL saline (BCG plus LPS). Alanine aminotransferase (ALT), aspartate aminotransferase (AST) in sera and malondialdehyde (MDA), glutathione peroxidase (GSHpx) contents in liver homogenates were assayed by spectrophotometry. The levels of tumor necrosis factor-~ (TNF-~) and nitric oxide (NO) levels in sera were determined using ELISA. Interleukin-1 (IL-1) produced by peritoneal macrophages was determined by the method of 3^H-infiltrated cell proliferation. The nuclear factor-kappa B (NF-xB) p65 in liver tissue was analyzed with reverse transcription polymerase chain reaction (RT-PCR). Liver samples collected were stained with hematoxylin and eosin.RFSULTS: FR167653 (50, 100, 150 mg/kg) could significantly decrease the serum transaminase (ALl', AS'I-) activity and MDA content in liver homogenate, and improve reduced GSHpx level of liver homogenate. Liver histopathological examinatdon showed FR167653 (100, 150 mg/kg) significantly reduced inflammatory cells infiltration and liver cells necrosis. FR167653 (50, 100, 150 mg/kg) significantly lowered TNF-α and NO levels in serum, and IL-1 produced by peritoneal macrophages. Moreover, expression of NF-xBmRNA in liver tissue of ILl induced by BCG plus LPS was significantly reduced by FR167653.CONCLUSION: All results showed that FR167653 had significant inhibitorv action an ILI in mice.
