Poly （4- methylphenoxyphosphnzene ） -graft-poly （ ethylene oxide ） （ PPZ-g- PEO ）, a novel amphiphilie grafting polymer was prepared via the Decker-Forster reaction. It is found that the graft efficiency increas...Poly （4- methylphenoxyphosphnzene ） -graft-poly （ ethylene oxide ） （ PPZ-g- PEO ）, a novel amphiphilie grafting polymer was prepared via the Decker-Forster reaction. It is found that the graft efficiency increased with extension of reaction time. Low molecular weight of poly （ ethylene oxide ） favored the grafting reaction. The grafted polymer has two different glass transition temperatures（ Tg） with those of pure poly（ 4-methyl- phenoxy-phopsphazene ） and PEO. The emulsifying ability of grafted polymer was studied with benzene-water mixtare. The emulsifying volumes increased with the decreasing of PEO＇ s molecular weight. The contact angle of film forming from grafted polymer decreased after introduction of PEO grafting chain.展开更多
This paper analyses the female characters in E.M.Forster’s novels from the perspective of feminism,through the deep exploration of E.M.Forster’s works,it is evident to draw a conclusion that there are four kinds of ...This paper analyses the female characters in E.M.Forster’s novels from the perspective of feminism,through the deep exploration of E.M.Forster’s works,it is evident to draw a conclusion that there are four kinds of female characters:first of all,stubborn and arrogant old ladies;the second type is insensitive but self-awakening middle-aged ladies;the third type is knowledgeable and smart young ladies;the last one is the warm-hearted and benevolent lady.These four kinds of different female characters can reveal that E.M.Forster has a contradictory attitude towards women.For one thing,he respected and sympathized with women,for another he discriminated against women.展开更多
Design Concept:Hanging Gardens The concept building’s primary structure consists of a central series of steel frames from which all floors are suspended.All apartment units are modular so that the configuration and a...Design Concept:Hanging Gardens The concept building’s primary structure consists of a central series of steel frames from which all floors are suspended.All apartment units are modular so that the configuration and aggregation of units can be adapted to market demands.The overall building is covered with a steel-mesh,allowing a space in between it and the building for balconies or service corridors.The steel mesh also provides a base for climbing plants,providing synthesis with an urban and natural landscape.设计理念:悬挂的花园建筑的主体结构为一个悬挂着其他楼层的主钢架群。所有的公寓单元都模块化,所以单元的布局及总数量可以根据市场需要进行调节。整个建筑被一个钢网罩住,在钢网与建筑中间还可布置阳台或维修通道。钢网上也可安置攀爬植物,同时提供城市和自然的景观,见图1。展开更多
Organic nanophotocatalysts are promising candidates for solar fuels production,but they still face the challenge of unfavorable geminate recombination due to the limited exciton diffusion lengths.Here,we introduce a b...Organic nanophotocatalysts are promising candidates for solar fuels production,but they still face the challenge of unfavorable geminate recombination due to the limited exciton diffusion lengths.Here,we introduce a binary nanophotocatalyst fabricated by blending two polymers,PS-PEG5(PS)and PBT-PEG5(PBT),with matched absorption and emission spectra,enabling a Forster resonance energy transfer(FRET)process for enhanced photocatalysis.These heterostructure nanophotocatalysts are processed using a facile and scalable flash nanoprecipitation(FNP)technique with precious kinetic control over binary nanoparticle formation.The resulting nanoparticles exhibit an exceptional photocatalytic hydrogen evolution rate up to 65 mmol g^(-1) h^(-1),2.5 times higher than that single component nanoparticles.Characterizations through fluorescence spectra and transient absorption spectra confirm the hetero-energy transfer within the binary nanoparticles,which prolongs the excited-state lifetime and extends the namely“effective exciton diffusion length”.Our finding opens new avenues for designing efficient organic photocatalysts by improving exciton migration.展开更多
Red fluorescent proteins with large Stokes shift(LSS-RFPs)are advantageous for multicolor imaging applications that allow simultaneous visualizations of multiple biological events.But it is difficult to develop LSS-RF...Red fluorescent proteins with large Stokes shift(LSS-RFPs)are advantageous for multicolor imaging applications that allow simultaneous visualizations of multiple biological events.But it is difficult to develop LSS-RFPs by extending the emission wavelength of RFPs to far-red region.Here,we employed Forster resonance energy transfer(FRET)strategy to engineer the far-red fluorescent proteins with large Stokes shift.LSS-m Apple and LSS-mCherry were constructed by fusing HaloTag to m Apple and mCherry,allowing the fluorophore TMSi R to be connected to these RFPs.FRET between RFPs and TMSi R enabled them to apply the excitation of donor RFPs to emit far-red fluorescence of acceptor TMSi R.The Stokes shifts of LSS-m Apple and LSS-mCherry were 97 nm and 75 nm,respectively.The high FRET efficiency of LSS-mCherry(E_(FRET)=83.7%)can greatly reduce the fluorescence from the donor channel,which did not affect co-imaging with mCherry.In addition,LSS-mCherry also showed excellent photostability(t_(1/2)=449.3 s),enabling stable confocal fluorescence imaging for 15 min under continuous strong excitation.Furthermore,LSS-mCherry was applied for fluorescence labeling and imaging of the nucleus,mitochondria,lysosomes,and endoplasmic reticulum in living cells.Finally,we applied LSS-mCherry to perform multi-color bioimaging of 2–4 channels,and there was no obvious crosstalk between these channels.展开更多
Multicharged supramolecular assemblies based on luminescent macrocycle play an important role in extending their optical properties and functions.Herein,we reported macrocyclic supramolecular assemblies based on lumin...Multicharged supramolecular assemblies based on luminescent macrocycle play an important role in extending their optical properties and functions.Herein,we reported macrocyclic supramolecular assemblies based on luminescent terphen[3]arene sulfate(TP[3]AS)and tetraphenylethylene pyridinium(TPE-4Py)through electrostatic interactions,host-vip encapsulation andπ-πstacking interactions.F?rster resonance energy transfer(FRET)process from TP[3]AS to TPE-4Py was achieved with the energy transfer efficiency of 99.9%,accompanied by TPE-4Py fluorescence emission bathochromic shifted of 15 nm and enhanced by 1.68 times in PBS solution.In contrast,other non-luminescent sulfato-β-cyclodextrin and sulfobutylether-β-cyclodextrin only can enhance the fluorescence intensity of TPE-4Py without bathochromic shift.Due to the strong fluorescence and good stability of TPE-4Py@TP[3]AS,it can be used for optical imaging in living cells,which provided an effective approach for the construction of assembling-confined luminescent biomaterials.展开更多
激发态能量转移(Excitation Energy Transfer,EET)作为一类重要的光物理现象,被广泛用于比率型荧光探针和分子灯标的设计以及DNA检测等多个领域.影响EET效率的两个重要因素是供受体间的空间距离和光谱交盖,通过调节供受体间的空间距离...激发态能量转移(Excitation Energy Transfer,EET)作为一类重要的光物理现象,被广泛用于比率型荧光探针和分子灯标的设计以及DNA检测等多个领域.影响EET效率的两个重要因素是供受体间的空间距离和光谱交盖,通过调节供受体间的空间距离或光谱重叠程度来调控能量转移过程,实现对目标客体的双波长比率检测.综述了基于不同供受体荧光团的EET体系、供受体间的连接方式对能量转移效率的影响,以及通过调控供受体间光谱重叠程度或空间距离,获得识别不同客体的比率型荧光探针,并对EET机理的比率型荧光探针的设计以及未来在生物成像和医学检测等领域的应用进行了展望.展开更多
文摘Poly （4- methylphenoxyphosphnzene ） -graft-poly （ ethylene oxide ） （ PPZ-g- PEO ）, a novel amphiphilie grafting polymer was prepared via the Decker-Forster reaction. It is found that the graft efficiency increased with extension of reaction time. Low molecular weight of poly （ ethylene oxide ） favored the grafting reaction. The grafted polymer has two different glass transition temperatures（ Tg） with those of pure poly（ 4-methyl- phenoxy-phopsphazene ） and PEO. The emulsifying ability of grafted polymer was studied with benzene-water mixtare. The emulsifying volumes increased with the decreasing of PEO＇ s molecular weight. The contact angle of film forming from grafted polymer decreased after introduction of PEO grafting chain.
文摘This paper analyses the female characters in E.M.Forster’s novels from the perspective of feminism,through the deep exploration of E.M.Forster’s works,it is evident to draw a conclusion that there are four kinds of female characters:first of all,stubborn and arrogant old ladies;the second type is insensitive but self-awakening middle-aged ladies;the third type is knowledgeable and smart young ladies;the last one is the warm-hearted and benevolent lady.These four kinds of different female characters can reveal that E.M.Forster has a contradictory attitude towards women.For one thing,he respected and sympathized with women,for another he discriminated against women.
文摘Design Concept:Hanging Gardens The concept building’s primary structure consists of a central series of steel frames from which all floors are suspended.All apartment units are modular so that the configuration and aggregation of units can be adapted to market demands.The overall building is covered with a steel-mesh,allowing a space in between it and the building for balconies or service corridors.The steel mesh also provides a base for climbing plants,providing synthesis with an urban and natural landscape.设计理念:悬挂的花园建筑的主体结构为一个悬挂着其他楼层的主钢架群。所有的公寓单元都模块化,所以单元的布局及总数量可以根据市场需要进行调节。整个建筑被一个钢网罩住,在钢网与建筑中间还可布置阳台或维修通道。钢网上也可安置攀爬植物,同时提供城市和自然的景观,见图1。
基金supported by National Natural Science Foundation of China(NSFC,22338006,92356301,9235630033 and 22375062)Shanghai Municipal Science and Technology Major Project(21JC1401700)+4 种基金Shanghai Pilot Program for Basic Research(22TQ1400100-10)Fundamental Research Funds for the Central UniversitiesShanghai Pujiang Program(22PJ1402400)“Chenguang Program”supported by Shanghai Education Development Foundation and Shanghai Municipal Education Commission(22CGA32)the Young Elite Scientists Sponsorship Program by CAST(2023QNRC001).
文摘Organic nanophotocatalysts are promising candidates for solar fuels production,but they still face the challenge of unfavorable geminate recombination due to the limited exciton diffusion lengths.Here,we introduce a binary nanophotocatalyst fabricated by blending two polymers,PS-PEG5(PS)and PBT-PEG5(PBT),with matched absorption and emission spectra,enabling a Forster resonance energy transfer(FRET)process for enhanced photocatalysis.These heterostructure nanophotocatalysts are processed using a facile and scalable flash nanoprecipitation(FNP)technique with precious kinetic control over binary nanoparticle formation.The resulting nanoparticles exhibit an exceptional photocatalytic hydrogen evolution rate up to 65 mmol g^(-1) h^(-1),2.5 times higher than that single component nanoparticles.Characterizations through fluorescence spectra and transient absorption spectra confirm the hetero-energy transfer within the binary nanoparticles,which prolongs the excited-state lifetime and extends the namely“effective exciton diffusion length”.Our finding opens new avenues for designing efficient organic photocatalysts by improving exciton migration.
基金supported by the National Natural Science Foundation of China(Nos.22225806,22078314,22278394,22378385)Dalian Institute of Chemical Physics(Nos.DICPI202142,DICPI202436)。
文摘Red fluorescent proteins with large Stokes shift(LSS-RFPs)are advantageous for multicolor imaging applications that allow simultaneous visualizations of multiple biological events.But it is difficult to develop LSS-RFPs by extending the emission wavelength of RFPs to far-red region.Here,we employed Forster resonance energy transfer(FRET)strategy to engineer the far-red fluorescent proteins with large Stokes shift.LSS-m Apple and LSS-mCherry were constructed by fusing HaloTag to m Apple and mCherry,allowing the fluorophore TMSi R to be connected to these RFPs.FRET between RFPs and TMSi R enabled them to apply the excitation of donor RFPs to emit far-red fluorescence of acceptor TMSi R.The Stokes shifts of LSS-m Apple and LSS-mCherry were 97 nm and 75 nm,respectively.The high FRET efficiency of LSS-mCherry(E_(FRET)=83.7%)can greatly reduce the fluorescence from the donor channel,which did not affect co-imaging with mCherry.In addition,LSS-mCherry also showed excellent photostability(t_(1/2)=449.3 s),enabling stable confocal fluorescence imaging for 15 min under continuous strong excitation.Furthermore,LSS-mCherry was applied for fluorescence labeling and imaging of the nucleus,mitochondria,lysosomes,and endoplasmic reticulum in living cells.Finally,we applied LSS-mCherry to perform multi-color bioimaging of 2–4 channels,and there was no obvious crosstalk between these channels.
基金the National Natural Science Foundation of China(Nos.21971192,21807038)the Tianjin Municipal Education Commission(No.2021KJ188)the China Postdoctoral Science Foundation(No.2021T140343)。
文摘Multicharged supramolecular assemblies based on luminescent macrocycle play an important role in extending their optical properties and functions.Herein,we reported macrocyclic supramolecular assemblies based on luminescent terphen[3]arene sulfate(TP[3]AS)and tetraphenylethylene pyridinium(TPE-4Py)through electrostatic interactions,host-vip encapsulation andπ-πstacking interactions.F?rster resonance energy transfer(FRET)process from TP[3]AS to TPE-4Py was achieved with the energy transfer efficiency of 99.9%,accompanied by TPE-4Py fluorescence emission bathochromic shifted of 15 nm and enhanced by 1.68 times in PBS solution.In contrast,other non-luminescent sulfato-β-cyclodextrin and sulfobutylether-β-cyclodextrin only can enhance the fluorescence intensity of TPE-4Py without bathochromic shift.Due to the strong fluorescence and good stability of TPE-4Py@TP[3]AS,it can be used for optical imaging in living cells,which provided an effective approach for the construction of assembling-confined luminescent biomaterials.
文摘激发态能量转移(Excitation Energy Transfer,EET)作为一类重要的光物理现象,被广泛用于比率型荧光探针和分子灯标的设计以及DNA检测等多个领域.影响EET效率的两个重要因素是供受体间的空间距离和光谱交盖,通过调节供受体间的空间距离或光谱重叠程度来调控能量转移过程,实现对目标客体的双波长比率检测.综述了基于不同供受体荧光团的EET体系、供受体间的连接方式对能量转移效率的影响,以及通过调控供受体间光谱重叠程度或空间距离,获得识别不同客体的比率型荧光探针,并对EET机理的比率型荧光探针的设计以及未来在生物成像和医学检测等领域的应用进行了展望.
