Hyperuricemia is a high-risk factor for the development of gout and renal fibrosis,but the adverse effects of hyperuricemia on the liver have been seriously neglected.This research investigated the ameliorating effect...Hyperuricemia is a high-risk factor for the development of gout and renal fibrosis,but the adverse effects of hyperuricemia on the liver have been seriously neglected.This research investigated the ameliorating effect of Lacticaseibacillus rhamnosus Fmb14 on hyperuricemia induced liver dysfunction both in vitro and in vivo.Cell free extracts of high dose L.rhamnosus Fmb14 treatment reduced the death rate of HepG2 cell lines from 24.1%to 14.9%by inhibiting NLRP3 recruitment,which was mainly activated by reactive oxygen species release and mitochondrial membrane potential disorder.In purine dietary induced hyperuricemia(PDIH)mice model,liver oedema and pyroptosis were ameliorated after L.rhamnosus Fmb14 administration through downregulating the expression levels of NLRP3,caspase-1 and gasdermin-D from 1.61 to 0.86,3.15 to 1.01 and 5.63 to 2.02,respectively.L.rhamnosus Fmb14 administration restored mitochondrial inner membrane protein(MPV17)and connexin 43 from 2.83 and 0.73 to 0.80 and 0.98 respectively in PDIH mice,indicating that dysbiosis of mitochondrial membrane potential was restored in liver.Intriguingly,PDIH pyroptosis stimulates the process of apoptosis,which leads to severe leakage of hepatocytes,and both of pyroptosis and apoptosis were decreased after L.rhamnosus Fmb14 treatment.Therefore,L.rhamnosus Fmb14 is a promising biological resource to maintain homeostasis of the liver in hyperuricemia and the prevention of subsequent complications.展开更多
Hyperuricemia is a critical threat to human health,and a high inosine diet can increase the prevalence of it.Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt,and its inosine de...Hyperuricemia is a critical threat to human health,and a high inosine diet can increase the prevalence of it.Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt,and its inosine degradation rate reached 36.3%at 109 CFU/mL for 24 h.LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L.rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin.The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts(CFE).L.rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase(XOD)inhibition and ATP-binding cassette subfamily G member 2(ABCG2)promotion,both of which are responsible for uric acid(UA)synthesis and secretion in HepG2 and Caco2 cells,respectively.The in vivo results showed that the serum UA level decreased from 236.28 to 149.28μmol/L after 8 weeks of oral administration of L.rhamnosus Fmb14 in inosine-induced hyperuricemia model mice.Our results revealed that L.rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention.展开更多
3月3日,由FMB(Fertilizer Market Business Group,UK.)举办的第二届亚洲会议和展览在北京香格里拉饭店举行,160多个国家和地区的代表参加了这次会议。作为全球化肥工业、化肥市场情报的主要提供者,FMB 周报监控、销售、咨询并定价所有...3月3日,由FMB(Fertilizer Market Business Group,UK.)举办的第二届亚洲会议和展览在北京香格里拉饭店举行,160多个国家和地区的代表参加了这次会议。作为全球化肥工业、化肥市场情报的主要提供者,FMB 周报监控、销售、咨询并定价所有的化肥产品,同时还向全球主要的生产商、销售商和顾客提供FMB周报。由于在去年一年内,中国化肥和原材料的供需趋势对全球的化肥行业都产生了十分巨大的影响,因此,会议的主要目的是对未来一年内的供需状况进行评估,并确定定价系统,探讨当前整个亚洲所面临的主要供需信息和分销信息。在为期两天会上,近10位国际性大公司的领导者针对不同的领域进行发言,与参会人员共同分享了在不同肥料类别以及运输、政策中的一些经验和做法,并对未来的市场进行了预测和分析。展开更多
铁路沿线彩钢房存在安装不规范、结构老化变形等问题,在大风天气下易被吹落至铁路线路附近,威胁列车行车安全,甚至可能引发严重安全事故。为解决上述问题,提出了一种基于无人机巡检图像的铁路沿线彩钢房状态检测算法。基于YOLOv10(You O...铁路沿线彩钢房存在安装不规范、结构老化变形等问题,在大风天气下易被吹落至铁路线路附近,威胁列车行车安全,甚至可能引发严重安全事故。为解决上述问题,提出了一种基于无人机巡检图像的铁路沿线彩钢房状态检测算法。基于YOLOv10(You Only Look Once,v10)算法的构架,首先,搭建了基于FasterNet优化的主干网络,提升原算法的特征提取能力,提高算法的检测精度;其次,构造新的C2F-FMB(Cross-Stage Partial Fusion-Feature Modulation Blocks)颈部模块,通过引入特征的自适应调制机制,增强算法对图像中局部细节和全局信息的提取能力,优化C2F模块在小目标检测的性能表现。结果表明:相比原始YOLOv10算法,改进后的算法在IoU(Intersection over Union)阈值为0.5时的平均精度均值(mean Average Precision,mAP@0.5)提高了4.7%。同时,该算法在小面积彩钢房检测任务以及具有相似特征的目标分类任务中表现出更强的适应性和更高的检测精度。展开更多
N-succinimidyl 4-[18F](fluoromethyl) benzoate for protein labeling was prepared (57%, EOB) in about 30min. Reaction conditions of S18FMB with IgG including pH of solutions, protein concentration, reaction temperature ...N-succinimidyl 4-[18F](fluoromethyl) benzoate for protein labeling was prepared (57%, EOB) in about 30min. Reaction conditions of S18FMB with IgG including pH of solutions, protein concentration, reaction temperature and time were studied. The optimal labeling conditions were: 0.2mg/mL IgG, pH = 7.8-8.5, 25℃, and reaction time 5min.Under these conditions the yield was about 80%. The 18F-labeled protein was purified by size exclusion chromatography.展开更多
基金Grant support was received from the National Natural Science Foundation of China(32072182).
文摘Hyperuricemia is a high-risk factor for the development of gout and renal fibrosis,but the adverse effects of hyperuricemia on the liver have been seriously neglected.This research investigated the ameliorating effect of Lacticaseibacillus rhamnosus Fmb14 on hyperuricemia induced liver dysfunction both in vitro and in vivo.Cell free extracts of high dose L.rhamnosus Fmb14 treatment reduced the death rate of HepG2 cell lines from 24.1%to 14.9%by inhibiting NLRP3 recruitment,which was mainly activated by reactive oxygen species release and mitochondrial membrane potential disorder.In purine dietary induced hyperuricemia(PDIH)mice model,liver oedema and pyroptosis were ameliorated after L.rhamnosus Fmb14 administration through downregulating the expression levels of NLRP3,caspase-1 and gasdermin-D from 1.61 to 0.86,3.15 to 1.01 and 5.63 to 2.02,respectively.L.rhamnosus Fmb14 administration restored mitochondrial inner membrane protein(MPV17)and connexin 43 from 2.83 and 0.73 to 0.80 and 0.98 respectively in PDIH mice,indicating that dysbiosis of mitochondrial membrane potential was restored in liver.Intriguingly,PDIH pyroptosis stimulates the process of apoptosis,which leads to severe leakage of hepatocytes,and both of pyroptosis and apoptosis were decreased after L.rhamnosus Fmb14 treatment.Therefore,L.rhamnosus Fmb14 is a promising biological resource to maintain homeostasis of the liver in hyperuricemia and the prevention of subsequent complications.
基金the National Natural Science Foundation of China(32072182).
文摘Hyperuricemia is a critical threat to human health,and a high inosine diet can increase the prevalence of it.Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt,and its inosine degradation rate reached 36.3%at 109 CFU/mL for 24 h.LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L.rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin.The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts(CFE).L.rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase(XOD)inhibition and ATP-binding cassette subfamily G member 2(ABCG2)promotion,both of which are responsible for uric acid(UA)synthesis and secretion in HepG2 and Caco2 cells,respectively.The in vivo results showed that the serum UA level decreased from 236.28 to 149.28μmol/L after 8 weeks of oral administration of L.rhamnosus Fmb14 in inosine-induced hyperuricemia model mice.Our results revealed that L.rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention.
文摘3月3日,由FMB(Fertilizer Market Business Group,UK.)举办的第二届亚洲会议和展览在北京香格里拉饭店举行,160多个国家和地区的代表参加了这次会议。作为全球化肥工业、化肥市场情报的主要提供者,FMB 周报监控、销售、咨询并定价所有的化肥产品,同时还向全球主要的生产商、销售商和顾客提供FMB周报。由于在去年一年内,中国化肥和原材料的供需趋势对全球的化肥行业都产生了十分巨大的影响,因此,会议的主要目的是对未来一年内的供需状况进行评估,并确定定价系统,探讨当前整个亚洲所面临的主要供需信息和分销信息。在为期两天会上,近10位国际性大公司的领导者针对不同的领域进行发言,与参会人员共同分享了在不同肥料类别以及运输、政策中的一些经验和做法,并对未来的市场进行了预测和分析。
文摘铁路沿线彩钢房存在安装不规范、结构老化变形等问题,在大风天气下易被吹落至铁路线路附近,威胁列车行车安全,甚至可能引发严重安全事故。为解决上述问题,提出了一种基于无人机巡检图像的铁路沿线彩钢房状态检测算法。基于YOLOv10(You Only Look Once,v10)算法的构架,首先,搭建了基于FasterNet优化的主干网络,提升原算法的特征提取能力,提高算法的检测精度;其次,构造新的C2F-FMB(Cross-Stage Partial Fusion-Feature Modulation Blocks)颈部模块,通过引入特征的自适应调制机制,增强算法对图像中局部细节和全局信息的提取能力,优化C2F模块在小目标检测的性能表现。结果表明:相比原始YOLOv10算法,改进后的算法在IoU(Intersection over Union)阈值为0.5时的平均精度均值(mean Average Precision,mAP@0.5)提高了4.7%。同时,该算法在小面积彩钢房检测任务以及具有相似特征的目标分类任务中表现出更强的适应性和更高的检测精度。
文摘N-succinimidyl 4-[18F](fluoromethyl) benzoate for protein labeling was prepared (57%, EOB) in about 30min. Reaction conditions of S18FMB with IgG including pH of solutions, protein concentration, reaction temperature and time were studied. The optimal labeling conditions were: 0.2mg/mL IgG, pH = 7.8-8.5, 25℃, and reaction time 5min.Under these conditions the yield was about 80%. The 18F-labeled protein was purified by size exclusion chromatography.
