For any cultured cell lines during their exponentially growing phase,the outflow rate of G2M cells can be measured with bromodeoxyuridine (BrdUrd) continuous labeling.The duration of G2M phase can be estibmated from t...For any cultured cell lines during their exponentially growing phase,the outflow rate of G2M cells can be measured with bromodeoxyuridine (BrdUrd) continuous labeling.The duration of G2M phase can be estibmated from the slope of the outtflow rate, The cell cycling time and the durations of G1 phase and S phase can be calculated from the measured fractions of G1, S cells and the cell age distribution theory. The method described here was simple and only about 1 hour was needed Several cell lines were measured and the obtained results were equivalent to the values determined by the FLSm curve or other methods.展开更多
文摘For any cultured cell lines during their exponentially growing phase,the outflow rate of G2M cells can be measured with bromodeoxyuridine (BrdUrd) continuous labeling.The duration of G2M phase can be estibmated from the slope of the outtflow rate, The cell cycling time and the durations of G1 phase and S phase can be calculated from the measured fractions of G1, S cells and the cell age distribution theory. The method described here was simple and only about 1 hour was needed Several cell lines were measured and the obtained results were equivalent to the values determined by the FLSm curve or other methods.
