Molecular imaging techniques are increasingly being used in the localization of disease, the staging of disease and for therapy control. The objective of current study focused on the optimization of synthesis, quality...Molecular imaging techniques are increasingly being used in the localization of disease, the staging of disease and for therapy control. The objective of current study focused on the optimization of synthesis, quality control, in vitro and in vivo evaluation of 123I radiopharmaceuticals based on the chemotactic peptide N-formyl-Met-Leu-Phe. Labeling studies were done both by direct method using chloramine-Y according to Khawli （1989） and indirect method using [125I and 131I] S1B according to Zalutsky （1987）. Then, biodistribution studies were performed both in normal mice and the one bearing 50 laL turpentine for 24h, promoted inflammation in right leg. Furthermore, the ability of the labeled peptide conjugate to bind to human polymorph nuclear leukocytes was determined using in vitro assay. With increasing in pl-l, yield of labeled FMLF （N-formyl-Met-Leu-Phe） decreased perhaps because of interaction OH to carboxyl group of SIB. The maximum activity was observed in the right leg which injected with turpentine due to infection and increase in blood circulation. Also, this peptide was conjugated to PMN （Poly morph nuclear） specifically and maximum activity was 66%. The highest absorption of FLMF was seen in kidney, liver, stomach and gut. The small size of this protein causes passing through the glomerular of kidney, so high activity was observed in urine and bladder.展开更多
文摘Molecular imaging techniques are increasingly being used in the localization of disease, the staging of disease and for therapy control. The objective of current study focused on the optimization of synthesis, quality control, in vitro and in vivo evaluation of 123I radiopharmaceuticals based on the chemotactic peptide N-formyl-Met-Leu-Phe. Labeling studies were done both by direct method using chloramine-Y according to Khawli （1989） and indirect method using [125I and 131I] S1B according to Zalutsky （1987）. Then, biodistribution studies were performed both in normal mice and the one bearing 50 laL turpentine for 24h, promoted inflammation in right leg. Furthermore, the ability of the labeled peptide conjugate to bind to human polymorph nuclear leukocytes was determined using in vitro assay. With increasing in pl-l, yield of labeled FMLF （N-formyl-Met-Leu-Phe） decreased perhaps because of interaction OH to carboxyl group of SIB. The maximum activity was observed in the right leg which injected with turpentine due to infection and increase in blood circulation. Also, this peptide was conjugated to PMN （Poly morph nuclear） specifically and maximum activity was 66%. The highest absorption of FLMF was seen in kidney, liver, stomach and gut. The small size of this protein causes passing through the glomerular of kidney, so high activity was observed in urine and bladder.
