目的评价激素样细胞因子-瘦素(leptin)与慢性特发性血小板减少性紫癜发病(CITP)的相关性。方法用ELISA法分别检测了46份CITP患者和40名健康志愿者血清瘦素水平。并且以外周血单个核细胞加入瘦素体外培养评价血小板抗体产生情况。数据以 ...目的评价激素样细胞因子-瘦素(leptin)与慢性特发性血小板减少性紫癜发病(CITP)的相关性。方法用ELISA法分别检测了46份CITP患者和40名健康志愿者血清瘦素水平。并且以外周血单个核细胞加入瘦素体外培养评价血小板抗体产生情况。数据以 student's t 检验处理。结果CITP患者平均血清瘦素水平(22.11±15.84ng/ml)明显高于健康志愿者(5.44±4.84ng/ml);瘦素水平阳性的CITP患者血小板数明显低于瘦素水平阴性的患者,而血小板相关抗原则明显高于后者。结论瘦素在慢性特发性血小板减少性紫癜发病机制中可能有重要作用,值得进一步探讨。展开更多
[目的]研究血清PECAM-1表达与胃癌患者预后的相关性,了解其在胃癌发生发展过程中的作用。[方法]入组初诊的Ⅳ期胃癌患者56例,经组织病理确诊、符合本研究入组标准,于入院后未经治疗前采取外周血5ml,运用酶联免疫法（ELISA法）检测外周血...[目的]研究血清PECAM-1表达与胃癌患者预后的相关性,了解其在胃癌发生发展过程中的作用。[方法]入组初诊的Ⅳ期胃癌患者56例,经组织病理确诊、符合本研究入组标准,于入院后未经治疗前采取外周血5ml,运用酶联免疫法（ELISA法）检测外周血中PECAM-1蛋白的表达,分析胃癌患者外周血中PECAM-1蛋白表达水平与预后及临床病理特征的关系。[结果]56例患者的中位总生存时间（m OS）为446d。胃癌患者血清PECAM-1中位值为79.5ng/ml,以该值作为分界值将患者分为高浓度组和低浓度组,PECAM-1高浓度组的m OS低于PECAM-1低浓度组（360d vs 558d,P=0.012）。PECAM-1高浓度组与低浓度组间患者性别、年龄（≥60岁与〈60岁）、一线化疗方案（XELOX与SOX）及周期数（≤4周期与〉4周期）、是否接受二线以上化疗差异均无统计学意义,但PECAM-1低浓度组一线化疗疗效优于高浓度组（P=0.043）。[结论]Ⅳ期胃癌患者血清PECAM-1水平与一线化疗疗效及预后生存可能相关,PECAM-1水平高者预后差,一线化疗疗效差。展开更多
Urea-soluble antigens of Trichinella spiralis infected larva were preparedand the efficiency of the antigens in diagnosis of trichinosis by ELISA wasstudied.Specific IgG antibodies were detected by ELISA using urea-so...Urea-soluble antigens of Trichinella spiralis infected larva were preparedand the efficiency of the antigens in diagnosis of trichinosis by ELISA wasstudied.Specific IgG antibodies were detected by ELISA using urea-solubleantigens in 91.67% (22/24) of 24 serum samples taken from patients withtrichinosis and 91.67% (11/12) of 12 blood spots on filter paper.Specific IgMantibodies were seen in 95.24% (20/21) of 21 serum samples from patients withtrichinosis using the antigens.Sera of patients infected with Schistosoma japonicumhad some cross teactions to the antigens,but the sera of mice infected withSchistosoma japoni(?) Ancylostoma caninum larva and Plasmodium yoelti were allnegative in the test.展开更多
文摘目的评价激素样细胞因子-瘦素(leptin)与慢性特发性血小板减少性紫癜发病(CITP)的相关性。方法用ELISA法分别检测了46份CITP患者和40名健康志愿者血清瘦素水平。并且以外周血单个核细胞加入瘦素体外培养评价血小板抗体产生情况。数据以 student's t 检验处理。结果CITP患者平均血清瘦素水平(22.11±15.84ng/ml)明显高于健康志愿者(5.44±4.84ng/ml);瘦素水平阳性的CITP患者血小板数明显低于瘦素水平阴性的患者,而血小板相关抗原则明显高于后者。结论瘦素在慢性特发性血小板减少性紫癜发病机制中可能有重要作用,值得进一步探讨。
文摘[目的]研究血清PECAM-1表达与胃癌患者预后的相关性,了解其在胃癌发生发展过程中的作用。[方法]入组初诊的Ⅳ期胃癌患者56例,经组织病理确诊、符合本研究入组标准,于入院后未经治疗前采取外周血5ml,运用酶联免疫法（ELISA法）检测外周血中PECAM-1蛋白的表达,分析胃癌患者外周血中PECAM-1蛋白表达水平与预后及临床病理特征的关系。[结果]56例患者的中位总生存时间（m OS）为446d。胃癌患者血清PECAM-1中位值为79.5ng/ml,以该值作为分界值将患者分为高浓度组和低浓度组,PECAM-1高浓度组的m OS低于PECAM-1低浓度组（360d vs 558d,P=0.012）。PECAM-1高浓度组与低浓度组间患者性别、年龄（≥60岁与〈60岁）、一线化疗方案（XELOX与SOX）及周期数（≤4周期与〉4周期）、是否接受二线以上化疗差异均无统计学意义,但PECAM-1低浓度组一线化疗疗效优于高浓度组（P=0.043）。[结论]Ⅳ期胃癌患者血清PECAM-1水平与一线化疗疗效及预后生存可能相关,PECAM-1水平高者预后差,一线化疗疗效差。
文摘Urea-soluble antigens of Trichinella spiralis infected larva were preparedand the efficiency of the antigens in diagnosis of trichinosis by ELISA wasstudied.Specific IgG antibodies were detected by ELISA using urea-solubleantigens in 91.67% (22/24) of 24 serum samples taken from patients withtrichinosis and 91.67% (11/12) of 12 blood spots on filter paper.Specific IgMantibodies were seen in 95.24% (20/21) of 21 serum samples from patients withtrichinosis using the antigens.Sera of patients infected with Schistosoma japonicumhad some cross teactions to the antigens,but the sera of mice infected withSchistosoma japoni(?) Ancylostoma caninum larva and Plasmodium yoelti were allnegative in the test.
