期刊文献+
共找到16,076篇文章
< 1 2 250 >
每页显示 20 50 100
A Pragmatic Study of Dialogues in Flipped in Light of Speech Act Theory
1
作者 JI Ming-ming 《Journal of Literature and Art Studies》 2025年第10期769-774,共6页
Flipped is a book written by American author Wendelin Van Draanen. It is a novel about young teenagers and was adapted into the famous film of the same name in 2010. The thesis employs speech acts, as pioneered by Joh... Flipped is a book written by American author Wendelin Van Draanen. It is a novel about young teenagers and was adapted into the famous film of the same name in 2010. The thesis employs speech acts, as pioneered by John Austin and further developed by John Searle, to investigate the influence of dialogues on characterization and plot development in Flipped. By exploring the theory of speech acts presented in dialogues between characters, the author deciphers the underlying intentions embodied in the dialogues and demonstrates the importance of the use of speech acts in dialogues in revealing the characters, driving the development of the plot and expressing the theme of the text. 展开更多
关键词 PRAGMATICS speech act theory flipped DIALOGUE literary effect
在线阅读 下载PDF
Aligning and flocking of ellipsoidal Quincke rollers by flipping
2
作者 Kai-Xuan He Qi-Ying Ni +3 位作者 Xiao-Yi Zhou Wen-De Tian Kang Chen Tian-Hui Zhang 《Chinese Physics B》 2025年第7期457-462,共6页
Active rods propelled along their long axis align their velocities and orientations simultaneously in collision.However,as the propulsion is perpendicular to the long axis,velocity alignment becomes dynamically diffic... Active rods propelled along their long axis align their velocities and orientations simultaneously in collision.However,as the propulsion is perpendicular to the long axis,velocity alignment becomes dynamically difficult.Here,we show that ellipsoidal Quincke roller propelled along their short-axis(perpendicular to the long axis)can align their velocities by flipping and form flocking with nematic order.The flipping arises from the reversible transition between the static parallel spinless state and the spinning transversal state of ellipsoidal Quincke rollers.This is possible only near(above)the critical field where both the parallel spinless state and the spinning transversal spinning are metastable.The flipping-facilitated alignment offers an extra aligning mechanism for elongate active agents,and the resulting active liquid crystals serve a model system to explore the defect dynamics as the propulsion deviates from the local nematic orientation which has not been addressed yet. 展开更多
关键词 Quincke roller FLOCKING ALIGNING flipPING
原文传递
Analysis of the Application Effect of Group Cooperative Learning Combined with Flipped Classroom in Standardized Training of Resident Physicians
3
作者 Wei Gong Zhi Li +1 位作者 Wei Dai Qiang Zhang 《Journal of Contemporary Educational Research》 2025年第8期260-267,共8页
Objective:To explore the application effect of group cooperative learning combined with flipped classroom in standardized training of emergency surgery resident physicians.Methods:95 resident physicians undergoing eme... Objective:To explore the application effect of group cooperative learning combined with flipped classroom in standardized training of emergency surgery resident physicians.Methods:95 resident physicians undergoing emergency surgery standardized training in our hospital were randomly divided into an experimental group(46,group cooperative learning+flipped classroom)and a control group(49,traditional teaching).The training period was 2 months.Results:The graduation assessment scores of the experimental group were higher than those of the control group(P<0.05).The improvement in critical thinking ability and self-learning ability was better than that of the control group(P<0.05).The response rate of“yes”to all items of course teaching satisfaction was significantly higher than that of the control group(P<0.05).The satisfaction scores for training teachers were higher than those of the control group(P<0.05).Conclusion:The teaching model of group cooperative learning combined with flipped classroom,through reconstructing the learning process and activating interactive participation,can significantly improve the clinical operation and theoretical foundation of emergency surgery standardized training students.It also cultivates core professional qualities such as critical thinking,self-learning,and team collaboration,providing a feasible paradigm for integrated medical education in this discipline. 展开更多
关键词 Emergency surgery NEUROLOGY Standardized training Group cooperative learning flipped classroom
在线阅读 下载PDF
“美丽小宠物”重塑AI体验——国民小折叠努比亚Flip2售价3399元起
4
《计算机应用文摘》 2025年第7期262-263,共2页
3月4日,“国民小折叠,真AI随声动”努比亚Flip 2正式发布。新机不仅是2025年行业首款全尺寸内嵌DeepSeek的小折叠手机,更以全语音AI萌宠重塑外屏语音交互新方式。其机身轻盈纤薄,AI影像和拍照玩法进一步升级,具备实用均衡的性能体验。作... 3月4日,“国民小折叠,真AI随声动”努比亚Flip 2正式发布。新机不仅是2025年行业首款全尺寸内嵌DeepSeek的小折叠手机,更以全语音AI萌宠重塑外屏语音交互新方式。其机身轻盈纤薄,AI影像和拍照玩法进一步升级,具备实用均衡的性能体验。作为2025年度首部小折叠,努比亚Flip 2为追求自我的年轻人提供全方位的陪伴,带来全新体验。 展开更多
关键词 努比亚 语音交互 AI 全尺寸 折叠 flip
在线阅读 下载PDF
Evolution mechanism of interconnect interface and shear properties of 64.8Sn35.2Pb microbump during flip chip bonding
5
作者 SHEN Yu-lu LUO Jiao +2 位作者 XU Keng-feng WU Dao-wei ZHANG Ning 《Journal of Central South University》 2025年第4期1284-1298,共15页
Effect of flip chip bonding parameters on microstructure at the interconnect interface and shear properties of 64.8Sn35.2Pb microbumps were investigated in this work.Results show that the main intermetallic compound(I... Effect of flip chip bonding parameters on microstructure at the interconnect interface and shear properties of 64.8Sn35.2Pb microbumps were investigated in this work.Results show that the main intermetallic compound(IMC)at the interconnect interface is(Ni,Cu)_(3)Sn_(4)phase,and meanwhile a small amount of(Cu,Ni)_(6)Sn_(5)phase with a size of 50−100 nm is formed around(Ni,Cu)_(3)Sn_(4)phase.The orientation relationship of[-1-56](Ni,Cu)_(3)Sn_(4)//[152](Cu,Ni)_(6)Sn_(5)and(601)(Ni,Cu)_(3)Sn_(4)//(-201)(Cu,Ni)_(6)Sn_(5)is found between these two phases,and the atomic matching at the interface of the two phases is low.The highest shear force of 77.3 gf is achieved in the 64.8Sn35.2Pb microbump at the peak temperature of 250℃and parameter V1 because dense IMCs and no cracks form at the interconnect interface.Two typical fracture modes of microbumps are determined as solder fracture and mixed fracture.The high thermal stress presenting in the thick IMCs layer induces crack initiation,and cracks propagate along theα/βphase boundaries in the Sn-Pb solder under shear force,leading to a mixed fracture mode in the microbumps. 展开更多
关键词 flip chip bonding microbump SN-PB intermetallic compound orientation relationship shear properties
在线阅读 下载PDF
Void Formation Analysis in the Molded Underfill Process for Flip-Chip Packaging
6
作者 Ian Hu Tzu-Chun Hung +2 位作者 Mu-Heng Zhou Heng-Sheng Lin Dao-Long Chen 《Computers, Materials & Continua》 2025年第7期537-551,共15页
Flip-chip technology is widely used in integrated circuit(IC)packaging.Molded underfill transfer molding is the most common process for these products,as the chip and solder bumps must be protected by the encapsulatin... Flip-chip technology is widely used in integrated circuit(IC)packaging.Molded underfill transfer molding is the most common process for these products,as the chip and solder bumps must be protected by the encapsulating material to ensure good reliability.Flow-front merging usually occurs during the molding process,and air is then trapped under the chip,which can form voids in the molded product.The void under the chip may cause stability and reliability problems.However,the flow process is unobservable during the transfer molding process.The engineer can only check for voids in the molded product after the process is complete.Previous studies have used fluid visualization experiments and developed computational fluid dynamics simulation tools to investigate this issue.However,a critical gap remains in establishing a comprehensive three-dimensional model that integrates two-phase flow,accurate venting settings,and fluid surface tension for molded underfill void evaluation—validated by experimental fluid visualization.This study aims to address this gap in the existing literature.In this study,a fluid visualization experiment was designed to simulate the transfer molding process,allowing for the observation of flow-front merging and void formation behaviors.For comparison,a three-dimensional mold flow analysis was also performed.It was found that the numerical simulation of the trapped air compression process under the chip was more accurate when considering the capillary force.The effect of design factors is evaluated in this paper.The results show that the most important factors for void size are fluid viscosity,the gap height under the chip,transfer time,contact angle between the fluid and the contact surfaces,and transfer pressure.Specifically,a smaller gap height beneath the chip aggravates void formation,while lower viscosity,extended transfer time,reduced contact angle,and increased transfer pressure are effective in minimizing void size.The overall results of this study will be useful for product and process design in selecting appropriate solutions for IC packaging,particularly in the development of void-free molded-underfill flip-chip packages.These findings support the optimization of industrial packaging processes in semiconductor manufacturing by guiding material selection and process parameters,ultimately enhancing package reliability and yield. 展开更多
关键词 flip chip transfer molding molded underfill void formation capillary force
在线阅读 下载PDF
Research on the Application of Information-Based Flipped Classroom Teaching Model in Nursing Education of Rheumatology and Immunology Department
7
作者 Yaqi Niu Xiaoyan Li +3 位作者 Ning Li Changjuan Li Na Li Hua Guo 《Journal of Contemporary Educational Research》 2025年第9期282-288,共7页
Objective:To explore the application effect of an information-based flipped classroom teaching model in nursing education of rheumatology and immunology,providing empirical evidence for optimizing specialized nursing ... Objective:To explore the application effect of an information-based flipped classroom teaching model in nursing education of rheumatology and immunology,providing empirical evidence for optimizing specialized nursing education models.Methods:Eighty nursing students from the 2021 grade of Xi’an Medical University were selected as research subjects and randomly divided into an experimental group(n=40)and a control group(n=40)using a random number table method.The control group received traditional lecture-based teaching,while the experimental group underwent information-based flipped classroom teaching.The theoretical knowledge scores and practical operation assessment scores of the two groups were compared after teaching,and the teaching satisfaction of the experimental group students was evaluated through a questionnaire survey.Results:The theoretical knowledge score(85.60±5.20)and practical operation score(88.30±4.70)of the experimental group were significantly higher than those of the control group(76.20±6.50)and(75.80±5.90),respectively.The differences were statistically significant(p<0.05).The satisfaction scores of all dimensions in the experimental group were significantly higher than those in the control group,and the differences were statistically significant(p<0.05).Conclusion:The information-based flipped classroom teaching model can effectively improve the quality of nursing teaching in rheumatology and immunology,enhance students’knowledge mastery and practical abilities,and is worthy of promotion and application in clinical nursing teaching. 展开更多
关键词 Information-based teaching flipped classroom Rheumatology and immunology Nursing education Teaching effectiveness
在线阅读 下载PDF
The learning expectations of undergraduate nursing students for the flipped Health Assessment course:A qualitative study
8
作者 Yixin Luo Jingjing You +4 位作者 Yimin Chen Meijing Chen Jiayuan Zhuang Fangli Xu Rong Hu* 《International Journal of Nursing Sciences》 2025年第5期485-492,I0004,共9页
Objective:This study aimed to investigate the learning expectations of undergraduate nursing students regarding the flipped Health Assessment course.Methods:This descriptive,qualitative study was conducted at a medica... Objective:This study aimed to investigate the learning expectations of undergraduate nursing students regarding the flipped Health Assessment course.Methods:This descriptive,qualitative study was conducted at a medical university in Fuzhou,Fujian Province,China.An interview outline was designed based on the core dimensions of Expectation Confirmation Theory(expectation sources,expectation content,and expectation importance).Thirty second-year undergraduate nursing students who had completed first-year basic medical courses and were about to take the flipped Health Assessment course were interviewed between June and July 2022.Interview data were analyzed using qualitative content analysis.Results:Five major themes and thirteen subthemes were identified.Theme 1 was expectation sourcesperceived learning difficulties from past experiences,which included four subthemes:insufficient autonomous learning ability,confusion regarding learning methods,insufficient engagement in learning,and low professional identity.Theme 2 included knowledge and information expectations,which comprised three subthemes:knowledge to improve professional competence,knowledge to enhance academic competitiveness,and knowledge to boost self-efficacy.Theme 3 comprised logical expectations and included two subthemes:flexible teaching methods and efficient instructional tools.Theme 4,pleasure expectations,included two subthemes:vivid teaching styles and diversified teaching evaluations.Theme 5 comprised professional value expectations and included two subthemes:teachers'responsible professional attitudes and gentle emotional support.Conclusion:Students'learning expectations originate from perceived learning difficulties,such as insufficient learning autonomy and engagement,confusion about learning methods,and a lack of professional identity.They showed various learning expectations for the flipped Health Assessment course,including knowledge and information,logic,pleasure,and professional value expectations. 展开更多
关键词 flipped course Health assessment Learning expectations Nursing education Nursing students Qualitative research
在线阅读 下载PDF
The Application of Flipped Classroom Teaching Mode Based on SPOC in College English Teaching:Taking the Zhihuishu Knowledge Graph Platform as an Example
9
作者 Yiying Li Yifan Liao 《Journal of Contemporary Educational Research》 2025年第9期27-37,共11页
In recent years,with the continuous development of educational digitalization,the integration model of SPOC(Small Private Online Course)and flipped classroom can integrate online teaching resources and offline teachin... In recent years,with the continuous development of educational digitalization,the integration model of SPOC(Small Private Online Course)and flipped classroom can integrate online teaching resources and offline teaching resources,overcome the shortcomings of traditional teaching models,and enhance students’enthusiasm for learning and autonomous learning ability.This model has become a popular direction in the reform of teaching models.Meanwhile,the advantages of the Zhihuishu knowledge graph platform,such as knowledge coherence and complete teaching modules,are highly beneficial for the promotion of the flipped classroom teaching model based on SPOC.This research will adopt methods such as literature review,investigation and research,questionnaire survey,and in-depth interview to analyze the application effect and optimization path of the flipped classroom teaching model based on SPOC on the Zhihuishu platform in college English teaching.The theoretical basis,practical effect and existing problems of the model were specifically analyzed,and corresponding optimization suggestions were put forward. 展开更多
关键词 Zhihuishu App SPOC flipped Classroom College English Teaching Digital education
在线阅读 下载PDF
The Effectiveness of Flipped Classroom Teaching in Surgical Nursing Education
10
作者 Su Tu 《Journal of Contemporary Educational Research》 2025年第11期183-190,共8页
Surgical nursing education has long grappled with a tension between traditional lecture-based models and actual practice needs.Students have difficulty converting theory into functional nursing skills in the passive f... Surgical nursing education has long grappled with a tension between traditional lecture-based models and actual practice needs.Students have difficulty converting theory into functional nursing skills in the passive format of knowledge acquisition.As medical education concepts have been renewed,the goals of educating nursing talents with independent judgment and adaptability to clinical practice have evolved into a significant direction of teaching reform.To this end,the“flipped classroom”model that blends lecture with discussion,through the mechanism of“internalized absorption”and“discussion”in the classroom,represents both a new teaching model that meets the need for integration of surgical nursing knowledge and an approach to skill application.This model reconstructs the teaching and learning process by providing a bridge for students to transfer from understanding knowledge to clinical thinking,thus providing a path forward for the dilemma of the separation of theory and practice in surgical nursing education. 展开更多
关键词 flipped classroom Surgical nursing teaching Application effect
在线阅读 下载PDF
Analysis of the Application of Flipped Classroom Teaching Mode in the Elementary Comprehensive Chinese Course
11
作者 Qiuyun Wang Weidi Xu Yuzhen Zhou 《Journal of Contemporary Educational Research》 2025年第10期415-420,共6页
The widespread adoption of the internet has provided new platforms and possibilities for Chinese language instruction.Students can utilize online resources or mobile devices for learning outside the classroom,while te... The widespread adoption of the internet has provided new platforms and possibilities for Chinese language instruction.Students can utilize online resources or mobile devices for learning outside the classroom,while teachers can shift the“intensive instruction”component of comprehensive Chinese courses to extracurricular settings.This approach enables increased practice time during class sessions,truly placing the learner at the center of the educational process.The flipped classroom model aligns with this philosophy and complements the disciplinary characteristics of comprehensive Chinese courses.In practice,implementing the O-PIRTAS universal flipped classroom model revealed its effectiveness in enhancing oral proficiency and overall competency.However,it is essential to concurrently address students’writing skills and cultivate their awareness of the flipped classroom approach. 展开更多
关键词 flipped classroom Teaching model Elementary comprehensive Chinese
在线阅读 下载PDF
Observation on the Effect of Internal Limiting Membrane Flipping and Covering Technique in the Treatment of High Myopic Macular Hole
12
作者 Zhiwei Li 《Journal of Clinical and Nursing Research》 2025年第5期245-251,共7页
Objective:To evaluate the effectiveness of the internal limiting membrane(ILM)flipping and covering technique in the treatment of patients with high myopic macular hole(HMMH).Methods:One hundred and two patients with ... Objective:To evaluate the effectiveness of the internal limiting membrane(ILM)flipping and covering technique in the treatment of patients with high myopic macular hole(HMMH).Methods:One hundred and two patients with HMMH who were admitted to the hospital from June 2019 to June 2024 were selected.The minimum diameter of the macular hole(MH)in all patients was≤500μm.The patients were randomly divided into two groups.The experimental group received 25G pars plana vitrectomy(PPV)combined with ILM flipping and covering technique,while the reference group received PPV combined with ILM peeling.The efficacy indicators and best corrected visual acuity levels were compared between the two groups.Results:The MH closure rate and retinal reattachment rate were higher in the experimental group than in the reference group at 3 and 6 months postoperatively(P<0.05).The best corrected visual acuity level was higher in the experimental group than in the reference group at 3 and 6 months postoperatively(P<0.05).The Chinese version of the Visual Function-Related Quality of Life Questionnaire-25(CVRQOL-25)score was higher in the experimental group than in the reference group at 3 and 6 months postoperatively(P<0.05).No serious complications were observed in both groups postoperatively,and there was no difference between the two groups(P>0.05).Conclusion:The ILM flipping and covering technique can improve the MH closure rate and retinal reattachment rate in patients with HMMH(diameter≤500μm),enhance the best corrected visual acuity level,and improve the quality of life related to visual function,with fewer postoperative complications. 展开更多
关键词 Internal limiting membrane flipping Internal limiting membrane peeling High myopic macular hole
暂未订购
Realization of all optical D flip-flop using LPhC based on beams interference method
13
作者 Asghar ASKARIAN Fariborz PARANDIN Arezoo SHEYKHIAN 《Optoelectronics Letters》 2025年第7期397-401,共5页
In this study,we design and numerically investigate a novel all optical D flip-flop(AODFF)based on linear photonic crystal(LPhC)structure that is composed of optical waveguides using the finite difference time domain(... In this study,we design and numerically investigate a novel all optical D flip-flop(AODFF)based on linear photonic crystal(LPhC)structure that is composed of optical waveguides using the finite difference time domain(FDTD)method.The proposed structure has the hexagonal close packed of 16×20 circular rods that are suspended in the air substrate with a lattice constant of 606 nm.The plane wave expansion(PWE)method is used to obtain the band diagram for AODFF at an operating wavelength of 1550 nm.The proposed optical flip-flop achieves a low delay time of 0.2 ps and a high contrast ratio(CR)of 10.33 dB.The main advantage of this design is that the input power as low as 1 mW/μm^(2) is sufficient for its operation,since no nonlinear rods are included.In addition,the footprint of the proposed AODFF is 100μm^(2),which is smaller compared to the structures reported in the literature,and it has a fast switching frequency of 5 Tbit/s. 展开更多
关键词 all optical d flip flop linear photonic crystal finite difference time domain plane wave expansion optical waveguides air substrate plane wave beams interference method
原文传递
FLIP在非小细胞肺癌中的表达及其与细胞凋亡的关系 被引量:9
14
作者 曹勇 李清泉 +1 位作者 钟立厚 王爱玲 《中国肺癌杂志》 CAS 2003年第1期51-54,共4页
目的 研究FLIP在非小细胞肺癌 (NSCLC)中的表达并观察其与细胞凋亡的关系。方法 采用免疫组化技术检测FLIP在 48例NSCLC和 16例肺良性疾病组织中的表达 ,并应用TUNEL技术检测 48例NSCLC的凋亡情况。结果 肺癌组织中FLIP的表达率为 83... 目的 研究FLIP在非小细胞肺癌 (NSCLC)中的表达并观察其与细胞凋亡的关系。方法 采用免疫组化技术检测FLIP在 48例NSCLC和 16例肺良性疾病组织中的表达 ,并应用TUNEL技术检测 48例NSCLC的凋亡情况。结果 肺癌组织中FLIP的表达率为 83 .3 3 % ( 4 0 /4 8) ,明显高于肺良性病变组织( 2 5 .0 0 % ) (P <0 .0 1)。FLIP的表达水平与TNM分期及淋巴结转移有密切关系 ,但与肿瘤组织学分型、分化程度无明显关系。FLIP在肺癌组织中的表达水平与癌细胞凋亡指数 (AI)未见明显相关性 (r =-0 .2 11,P >0 .0 5 )。结论 FLIP的过量表达在NSCLC的发生发展中起重要作用 ,但与细胞凋亡无明显关系。 展开更多
关键词 flip 非小细胞肺癌 表达 细胞凋亡 免疫组化技术 TUNEL技术
暂未订购
凋亡抑制蛋白c-FLIP在带状疱疹患者细胞免疫中作用的研究 被引量:8
15
作者 陶娟 董婧 +3 位作者 李延 刘业强 杨井 涂亚庭 《中国皮肤性病学杂志》 CAS 北大核心 2008年第4期207-210,共4页
目的探讨凋亡抑制蛋白c-FLIP在抗水痘-带状疱疹病毒(VZV)免疫中的作用。方法采用流式细胞术检测30例无恶性肿瘤的带状疱疹患者、17例伴恶性肿瘤的带状疱疹患者和20例正常对照者外周血T细胞和B细胞内c-FLIP蛋白表达阳性率和CD3+T细胞阳... 目的探讨凋亡抑制蛋白c-FLIP在抗水痘-带状疱疹病毒(VZV)免疫中的作用。方法采用流式细胞术检测30例无恶性肿瘤的带状疱疹患者、17例伴恶性肿瘤的带状疱疹患者和20例正常对照者外周血T细胞和B细胞内c-FLIP蛋白表达阳性率和CD3+T细胞阳性率。结果急性期伴有恶性肿瘤的带状疱疹患者外周血T细胞内c-FLIP表达明显低于无恶性肿瘤和正常对照组(P<0.05,P<0.01),无恶性肿瘤组明显低于正常对照组(P<0.05);恢复期无恶性肿瘤的带状疱疹患者外周血T细胞内c-FLIP表达明显增加,与急性期相比有明显差异(P<0.01),但恢复期的伴恶性肿瘤和急性期相比无明显差异(P>0.05);外周血B细胞内c-FLIP表达在急性期和恢复期的带状疱疹患者中无明显差异(P>0.05)。CD3+T细胞在带状疱疹患者外周血中的阳性率与c-FLIP有相似的表达趋势,两者呈正相关(r分别为0.806和0.534,P均<0.05)。结论凋亡抑制蛋白c-FLIP在急性期带状疱疹患者外周血T细胞内低表达,恢复期则高表达,且与T细胞数量明显正相关,表明其可能参与带状疱疹患者T细胞的增殖,在带状疱疹发生、发展中发挥一定的作用。 展开更多
关键词 C—flip 带状疱疹 T淋巴细胞 增殖
暂未订购
凋亡相关蛋白FLIP和NF-κB在人骨肉瘤中表达及其与凋亡的关系研究 被引量:5
16
作者 付文娟 赵济华 +2 位作者 甘洪全 翟宏芳 李琪佳 《中国全科医学》 CAS CSCD 北大核心 2012年第17期1959-1961,共3页
目的探讨FLIP、NF-κB蛋白在人骨肉瘤组织中的表达以及与骨肉瘤细胞凋亡的关系。方法免疫组化方法检测FLIP、NF-κB蛋白在骨肉瘤及骨软骨瘤中的表达;用TUNEL方法通过激光扫描共聚焦显微镜检测骨肉瘤及骨软骨瘤细胞凋亡并计算细胞凋亡指... 目的探讨FLIP、NF-κB蛋白在人骨肉瘤组织中的表达以及与骨肉瘤细胞凋亡的关系。方法免疫组化方法检测FLIP、NF-κB蛋白在骨肉瘤及骨软骨瘤中的表达;用TUNEL方法通过激光扫描共聚焦显微镜检测骨肉瘤及骨软骨瘤细胞凋亡并计算细胞凋亡指数(AI)。结果骨肉瘤组织FLIP蛋白表达强度(0.276±0.013)高于骨软骨瘤(0.171±0.007)(t=26.122,P<0.05)。NF-κB蛋白表达也高于骨软骨瘤〔(0.754±0.026)和(0.318±0.009)〕(t=56.700,P<0.05)。骨肉瘤中AI显著低于骨软骨瘤(P<0.01)。骨肉瘤中FLIP蛋白表达与细胞凋亡指数呈负相关(r=-0.949,P<0.01);NF-κB蛋白表达与细胞凋亡指数之间也呈负相关(r=-0.877,P<0.01)。结论 FLIP、NF-κB均参与细胞凋亡的调控过程,二者均可抑制骨肉瘤细胞凋亡;骨肉瘤组织中FLIP及NF-κB蛋白均呈过表达,对其发生、发展中起促进作用。 展开更多
关键词 骨肉瘤 flip NF-ΚB 凋亡
暂未订购
蟾毒灵通过下调c-Flip和XIAP增强TRAIL诱导的乳腺癌MDA-MB-231细胞凋亡 被引量:5
17
作者 闫顺朝 曲秀娟 +3 位作者 邹华伟 焦昕 滕月娥 刘云鹏 《现代肿瘤医学》 CAS 2014年第8期1748-1752,共5页
目的:研究蟾毒灵(bufalin)对TRAIL诱导乳腺癌MDA-MB-231细胞凋亡的的影响及机制。方法:MTT法测定细胞活力、采用流式细胞仪检测细胞凋亡,免疫荧光实验检测DR4、DR5的表达情况,Western blot法检测c-Flip和XIAP蛋白的表达。结果:MTT结果显... 目的:研究蟾毒灵(bufalin)对TRAIL诱导乳腺癌MDA-MB-231细胞凋亡的的影响及机制。方法:MTT法测定细胞活力、采用流式细胞仪检测细胞凋亡,免疫荧光实验检测DR4、DR5的表达情况,Western blot法检测c-Flip和XIAP蛋白的表达。结果:MTT结果显示bufalin作用于MDA-MB-231细胞后24h和48h的IC50分别为934nmol/L和513nmol/L。流式分析显示50nmol/L的bufalin作用24h仅诱导4.5%的MDA-MB-231细胞凋亡,100ng/ml的TRAIL作用24h诱导5.1%的MDA-MB-231细胞凋亡,50nmol/L的bufalin与100ng/ml的TRAIL联合作用24小时后可诱导32.5%的MDA-MB-231细胞凋亡。Western blot分析显示bufalin与TRAIL联合作用后导致c-Flip和XIAP的明显下调。结论:Bufalin可通过下调cFLIP和XIAP的表达增强TRAIL诱导的乳腺癌MDA-MB-231细胞凋亡。 展开更多
关键词 BUFALIN TRAIL 乳腺癌 c—flip XIAP
暂未订购
FLIP基因在乳腺癌组织中表达的初步研究 被引量:3
18
作者 臧凤琳 张霖 +2 位作者 肖绪祺 于泳 孙保存 《中国肿瘤临床》 CAS CSCD 北大核心 2007年第6期308-311,共4页
目的:探讨FLIP基因(包括蛋白和mRNA水平)在乳腺癌组织中的表达及对乳腺癌生物学行为的影响。方法:采用免疫组化和荧光实时定量PCR技术,检测乳腺浸润性导管癌和正常乳腺组织中FLIP蛋白及mRNA的表达。结果:FLIPL/S蛋白在浸润性导管癌中的... 目的:探讨FLIP基因(包括蛋白和mRNA水平)在乳腺癌组织中的表达及对乳腺癌生物学行为的影响。方法:采用免疫组化和荧光实时定量PCR技术,检测乳腺浸润性导管癌和正常乳腺组织中FLIP蛋白及mRNA的表达。结果:FLIPL/S蛋白在浸润性导管癌中的平均染色积分为6.13±0.34,在正常乳腺组织中的平均染色积分为2.68±0.30,两组有显著性差异(P<0.05)。FLIPL、FLIPS mRNA在浸润性导管癌中的含量均高于正常乳腺组织(P<0.05)。结论:FLIP在乳腺癌组织中高表达,可能通过阻断凋亡通路参与乳腺癌的发生。 展开更多
关键词 乳腺癌 flip凋亡 实时定量PCR 免疫组化
暂未订购
c-FLIP(L)在乳腺癌中表达的意义及对凋亡的调节 被引量:3
19
作者 臧凤琳 魏熙胤 +2 位作者 孙蕾娜 赵秀兰 孙保存 《中国肿瘤临床》 CAS CSCD 北大核心 2010年第15期841-845,共5页
目的:检测乳腺浸润性导管癌组织中c-FLIP(L)蛋白的表达,探讨c-FLIP(L)在乳腺癌中的临床病理意义,以及对乳腺癌细胞凋亡、增殖的影响。方法:收集116例乳腺浸润性导管癌标本,应用免疫组化技术分析c-FLIP(L)与乳腺癌临床病理因素的相关性。... 目的:检测乳腺浸润性导管癌组织中c-FLIP(L)蛋白的表达,探讨c-FLIP(L)在乳腺癌中的临床病理意义,以及对乳腺癌细胞凋亡、增殖的影响。方法:收集116例乳腺浸润性导管癌标本,应用免疫组化技术分析c-FLIP(L)与乳腺癌临床病理因素的相关性。以TUNEL标记肿瘤细胞凋亡指数,Ki-67标记增殖指数,分析c-FLIP(L)与细胞凋亡、增殖的相关性。结果:c-FLIP(L)蛋白定位于浸润性导管癌癌细胞胞浆中,ER、PR、Ki-67定位于肿瘤细胞胞核,Her-2定位于肿瘤细胞胞膜,TUNEL染色定位于肿瘤细胞胞核。c-FLIP(L)蛋白高表达与患者低年龄(<35岁)、绝经前、未发生远处转移、较早的pTNM分期(Ⅰ~Ⅱ期)密切相关:与家族史、手术方式、肿瘤大小、淋巴结转移、组织学分级和肿瘤复发等临床病理因素不存在显著相关性。在总病例中,ER阳性64例(55.17%),PR阳性43例(37.07%),Her-2阳性19例(16.38%)。c-FLIP(L)高表达与ER、PR之间存在显著相关性(P<0.05),与Her-2表达无关。c-FLIP(L)蛋白表达与乳腺癌凋亡指数正相关(r=0.544,P<0.05),虽然呈现c-FLIP(L)表达越高增殖指数越低的趋势,但二者之间相关系数无统计学意义(r=0.148,P>0.05)。结论:在乳腺癌中,c-FLIP(L)阳性表达提示患者较为良好的临床预后,该因子可能直接或间接参与肿瘤细胞凋亡过程,具有进一步研究的价值。 展开更多
关键词 c—flip(L) 乳腺癌 临床病理意义 凋亡
暂未订购
c-FLIP在DATS诱导人胃癌SGC-7901细胞凋亡中的作用 被引量:3
20
作者 陈真伟 凌晖 +4 位作者 李振峰 段海瑞 胡程 文玲 朱亚平 《现代生物医学进展》 CAS 2010年第21期4187-4191,共5页
目的:探讨二烯丙基三硫(diallyl trisulfide,DATS)诱导人胃癌SGC-7901细胞凋亡及凋亡过程中c-FLIP的变化及意义。方法采用MTT、western-blot和细胞免疫组化分别检测DATS对SGC-7901细胞的增殖抑制率及c-FLIP的表达情况。光学显微镜观察... 目的:探讨二烯丙基三硫(diallyl trisulfide,DATS)诱导人胃癌SGC-7901细胞凋亡及凋亡过程中c-FLIP的变化及意义。方法采用MTT、western-blot和细胞免疫组化分别检测DATS对SGC-7901细胞的增殖抑制率及c-FLIP的表达情况。光学显微镜观察凋亡形态,流式细胞术检测凋亡率。结果:MTT结果显示,不同浓度DATS(6、8、10、12、14、16mg.L-1)DATS处理SGC-7901细胞24、48小时后,生长抑制率分别为20.4%--79%和36%--90%,DATS抑制作用随浓度及时间逐渐增强(P<0.05)。细胞免疫组化和western-blot显示:9.5mg..L-1DATS处理SGC-7901细胞24、48小时后,与对照组相比,c-FLIP的表达下调(P<0.05)。光学显微镜:通过9.5mg..L-1DATS作用后24、48小时后,胃癌细胞出现了凋亡形态学改变。流式细胞术检测:经过9.5mg..L-1DATS处理SGC-7901细胞24、48小时后,细胞凋亡率逐渐升高。结论:DATS促进SGC-7901细胞凋亡的机制可能与抑制c-FLIP蛋白的表达有关。 展开更多
关键词 二烯丙基三硫 SGC-7901细胞 c—flip 凋亡
原文传递
上一页 1 2 250 下一页 到第
使用帮助 返回顶部