[Objectives]Based on spectrophotometry,a method for determining the total flavonoid content in milk powder was established by optimizing sample pretreatment conditions,and method validation was performed.[Methods]Tota...[Objectives]Based on spectrophotometry,a method for determining the total flavonoid content in milk powder was established by optimizing sample pretreatment conditions,and method validation was performed.[Methods]Total flavonoids in milk powder were extracted with ethanol using 45℃ water bath ultrasonication for 60 min.Flavonoids contain a benzopyran ring structure and react with aluminum nitrate in a potassium acetate environment to form a yellow complex.The absorbance of this complex was measured at 420 nm for quantitative determination.[Results]The method exhibited a good linear relationship within the range of 0.2-1.0 mg.The limit of detection(LOD)was 0.05 g/100 g,and the limit of quantification(LOQ)was 0.3 g/100 g.When the spiked amount of total flavonoids was in the range of 0.3% to 1.0%,the spike recovery rates were 99.3% to 103.4%,and the relative standard deviations(RSD s)were less than 3.0%.[Conclusions]This method offers advantages such as rapidness,accuracy,good stability,and high sensitivity,and can be used for the detection of total flavonoid content in milk powder.展开更多
Chronic obstructive pulmonary disease(COPD)has been a major global public health issue due to its high prevalence,disability,and mortality rates.The pathogenesis of COPD is complex and remains incompletely understood,...Chronic obstructive pulmonary disease(COPD)has been a major global public health issue due to its high prevalence,disability,and mortality rates.The pathogenesis of COPD is complex and remains incompletely understood,compounded by a lack of specific and effective clinical therapies.Key pathophysiological mechanisms include oxidative stress,inflammation,programmed cell death,and fibrosis,influenced by external risk factors such as cigarette smoke and internal factors like immune deficiency.Natural flavonoids emerge as promising adjuvant treatments or potential drug candidates for COPD,attributed to their multi-target properties and low toxicity.This article provides an overview of various types and sources of natural flavonoids that exhibit therapeutic effects on COPD,their specific pharmacological actions and detailed mechanisms of action.This review aims to serve as a reference for adjuvant treatment strategies in daily dietary practices and to inspire novel drug candidates for COPD.展开更多
Coreopsis tinctoria Nutt.,an edible flowering plant,belongs to the Chrysanthemum family and is mainly grown at high altitudes in Northwestern China.It is rich in polyphenolic compounds,particularly marein and flavomar...Coreopsis tinctoria Nutt.,an edible flowering plant,belongs to the Chrysanthemum family and is mainly grown at high altitudes in Northwestern China.It is rich in polyphenolic compounds,particularly marein and flavomarein,and possesses multiple health-promoting properties,such as antioxidant,hypoglycemic and vasorelaxant effects.Previous bioactivity investigations majorly focused on C.tinctoria and its crude extract.The aim of the present study was to prepare marein-dominant C.tinctoria flavonoids(CF),further investigate the CF protective effects of liver fibrosis induced by carbon tetrachloride and elucidate the associated molecular mechanisms.Results have demonstrated that CF effectively attenuated hepatofibrogenesis by increasing the activity of glutathione(GSH)and superoxide dismutase(SOD);suppressing the hepatic stellate cell(HSC)activation,inhibiting transforming growth factorβ(TGF-β)activation and the production ofα-smooth muscle actin(α-SMA),alleviating the phosphorylation of extracellular signal-regulated kinases1/2(ERK1/2)and small mothers against decapentaplegic1/2(Smad1/2),thus maintaining the collagen metabolic homeostasis in the liver.Our study revealed that CF possesses an efficacious protective effect against chronic hepatic fibrosis due to their strong inhibitory effects of oxidative stress and chronic inflammation.展开更多
The intricate landscape of neurodegenerative diseases complicates the search for effective therapeutic approaches.Photoreceptor degeneration,the common endpoint in various retinal diseases,including retinitis pigmento...The intricate landscape of neurodegenerative diseases complicates the search for effective therapeutic approaches.Photoreceptor degeneration,the common endpoint in various retinal diseases,including retinitis pigmentosa and age-related macular degeneration,leads to vision loss or blindness.While primary cell death is driven by genetic mutations,oxidative stress,and neuroinflammation,additional mechanisms contribute to disease progression.In retinitis pigmentosa,a multitude of genetic alterations can trigger the degeneration of photoreceptors,while other retinopathies,such as agerelated macular degeneration,are initiated by combinations of environmental factors,such as diet,smoking,and hypertension,with genetic predispositions.Nutraceutical therapies,which blend the principles of nutrition and pharmaceuticals,aim to harness the health benefits of bioactive compounds for therapeutic applications.These compounds generally possess multi-target effects.Polyphenols and flavonoids,secondary plant metabolites abundant in plant-based foods,are known for their antioxidant,neuroprotective,and anti-inflammatory properties.This review focuses on the potential of polyphenols and flavonoids as nutraceuticals to treat neurodegenerative diseases such as retinitis pigmentosa.Furthermore,the importance of developing reliable delivery methods to enhance the bioavailability and therapeutic efficacy of these compounds will be discussed.By combining nutraceuticals with other emerging therapies,such as genetic and cell-based treatments,it is possible to offer a more comprehensive approach to treating retinal degenerative diseases.These advancements could lead to a viable and accessible option,improving the quality of life for patients with retinal diseases.展开更多
[Objectives]To explore the effects of fermentation process on the content and functions of flavonoids in Gardenia jasminoides.[Methods]G.jasminoides was fermented by microorganisms,and the fermentation process of tota...[Objectives]To explore the effects of fermentation process on the content and functions of flavonoids in Gardenia jasminoides.[Methods]G.jasminoides was fermented by microorganisms,and the fermentation process of total flavonoids from G.jasminoides was optimized,and the antioxidant activity and hyaluronidase inhibitory activity of the fermentation broth were tested.[Results]The best strain for fermentation of total flavonoids in G.jasminoides was Bacillus subtilis.The optimum fermentation conditions were as follows:the solid-liquid ratio was 1:30,the inoculation amount was 2%,and the fermentation time was 24 h.Under these fermentation conditions,the content of total flavonoids in G.jasminoides reached 36.90 mg/g,which was 45.22%higher than that of the control group without microbial fermentation,and it had good DPPH free radical and hydroxyl free radical scavenging ability,and the inhibition ability of hyaluronidase after fermentation was also improved.[Conclusions]This study provides a technical reference for the comprehensive application of G.jasminoides.展开更多
Inflammatory bowel disease(IBD)is a chronic gastrointestinal disease with a high incidence.Treatment for IBD includes medications and diet,and common anti-inflammatory medications have limitations like drug resistance...Inflammatory bowel disease(IBD)is a chronic gastrointestinal disease with a high incidence.Treatment for IBD includes medications and diet,and common anti-inflammatory medications have limitations like drug resistance and serious adverse effects.Accumulating evidence has demonstrated that dietary flavonoids exhibit an alleviative effect on IBD by influencing gut microbiota.The microbiota-derived metabolites also regulate IBD and maintain intestinal homeostasis.In this review,we investigate the therapeutic effect of gut microbiota and metabolites on IBD by intestinal immune and intestinal barrier function.We demonstrate the underlying mechanism of dietary flavonoids as an anti-inflammatory molecule alleviating IBD by regulating gut microbiota,short chain fatty acid(SCFA),bile acid(BA),tryptophan(Trp)metabolism and lipopolysaccharides(LPS)-toll-like receptor 4(TLR4)signaling pathway.Based on structural differences of flavonoids,we summarize the recent research progress on the role of different dietary flavonoids in alleviating IBD by gut microbiota and metabolites in animal and clinical trials.This review indicates that dietary flavonoids targeting gut microbiota and metabolites provide a promising strategy for the treatment of inflammation and novel insights into the management of IBD.展开更多
[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was per...[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was performed using ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)with 0.1%formic acid water(A)-acetonitrile(B)with gradient elution at 30℃,injection volume of 2μL,and flow rate of 0.3 mL/min.Electrospray ion source adopted positive and negative ion detection mode with scanning range m/z 100-1000.[Results]A total of 93 compounds were identified from MITF,including 57 flavonoids,25 phenolic acids,9 alkaloids and 2 others.Among them,dimethoxytaxifolin was identified as a possible new compound by SciFinder search,and 67 compounds were first identified in M.integrifolia.[Conclusions]This study provides a scientific foundation for clarifying the material basis of the efficacy of M.integrifolia and improving the quality standards.展开更多
This study aims to explore the mechanism by which flavonoids in Crataegus pinnatifida fruit improve Alzheimer’s disease(AD)through network pharmacology and molecular docking technology.The flavonoid components presen...This study aims to explore the mechanism by which flavonoids in Crataegus pinnatifida fruit improve Alzheimer’s disease(AD)through network pharmacology and molecular docking technology.The flavonoid components present in Crataegus pinnatifida fruit were gathered from the HERB,HIT,and ETCM databases,and were further supplemented by relevant published literature.The PubChem and SwissTargetPrediction databases were utilized to predict potential targets,and a“Crataegus pinnatifida fruit-active ingredient-target”network was constructed using Cytoscape 3.9.0 software.The GeneCards database was utilized to identify targets associated with AD,which were subsequently intersected with the active targets of Crataegus pinnatifi da fruit.A protein-protein interaction(PPI)network was constructed using the STRING platform.KEGG enrichment analysis of the core targets was conducted on an online bioinformatics mapping platform,while molecular docking of the primary active components and core targets was executed using AutoDock software.Eight flavonoids and 160 potential targets were identifi ed from Crataegus pinnatifi da fruit,of which 147 targets were linked to AD.The results of the“Crataegus pinnatifi da fruit-active ingredient-target”network indicated that quercetin was the principal flavonoid active ingredient.PPI analysis revealed that SRC and EGFR were the key targets,and KEGG analysis identifi ed the main enrichment pathways as Pathways in cancer,PI3K/Akt signaling pathway,and Proteoglycans in cancer.Molecular docking confi rmed the strong binding affi nity between the core targets and the primary active ingredient.The interaction of quercetin with the key targets SRC and EGFR may represent a signifi cant mechanism by which flavonoids from Crataegus pinnatifi da fruit contribute to the improvement of AD.展开更多
Vitex negundo(V.negundo),an Indian herb with a rich historical background for the handling of various complaints,is a member of the Verbenaceae family and is characterized by small trees with pale gray bark.This herb ...Vitex negundo(V.negundo),an Indian herb with a rich historical background for the handling of various complaints,is a member of the Verbenaceae family and is characterized by small trees with pale gray bark.This herb is widely used and has been recognized in traditional medicine for its pharmacological effects on a wide range of diseases.All sections of the plant,but particularly the leaves,contain a variety of secondary metabolites,including alkaloids,phenols,flavonoids,glycoside iridoids,tannins,and terpenes.The system is included in a number of store-bought herbal preparations and has the potential to function as an efficient bio-committee.Cancer continues to be a major cause of death and morbidity in spite of the intervention.One of the leading causes of death is cancer,and current therapies can have unpleasant side effects.Unhindered reactions,resistance to traditional cancer medications,radiation therapy,chemotherapy,and restricted access to tumor tissue are some of the reasons why treatment frequently fails.To reduce side effects,increase chemotherapy sensitivity,and slow the spread of cancer,new approaches are required.Small food molecules have been suggested in numerous research as supplemental therapies for cancer patients.The effectiveness of the flavonoid-rich V.negundo extract in treating cancer was assessed mechanically in this investigation.Total flavonoids have been isolated for qualitative phytochemical investigation using V.negundo ethanolic extract.This article highlights significant developments in this field and explores how flavonoids contribute to the targeted suppression of the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin pathway in different cancer types.展开更多
Background:The study aimed to investigate the protective effect and mechanism of total flavonoids of Scutellaria baicalensis(TFSB)on acute myocardial ischemia(AMI)rats by using functional metabonomics.Methods:Rats wer...Background:The study aimed to investigate the protective effect and mechanism of total flavonoids of Scutellaria baicalensis(TFSB)on acute myocardial ischemia(AMI)rats by using functional metabonomics.Methods:Rats were divided into the Control,Model,AMI positive control(Propranolol hydrochloride,30 mg/kg),low dose TFSB(50 mg/kg),and high dose TFSB(100 mg/kg)groups.Rats received the corresponding treatment by intragastric administration once daily for 10 consecutive days.Electrocardiogram,myocardial enzyme,triphenyltetrazolium chloride staining,hematoxylin-eosin,and enzyme-linked immunosorbent assay were performed to evaluate the protective effect of TFSB on AMI rats.Then,the UHPLC-Q-Orbitrap MS method based on serum metabolomics was utilised to search for metabolic biomarkers and metabolic pathways.Subsequently,Western blot and RT-PCR techniques were employed to identify the respective genes and proteins.Results:Pharmacodynamics revealed that TFSB could ameliorate AMI in rats.The results of the metabolomics analysis indicated that the alterations in metabolic profile observed in rats with AMI were partially improved by treatment with TFSB.Moreover,the mRNA expression levels of 5-lipoxygenase(5-LOX)and 15-lipoxygenase(15-LOX)and the protein expression levels of 5-LOX,15-LOX,interleukin-1β(IL-1β),and NF-κB p65 were reduced following treatment with TFSB.Conclusion:The potential treatment of TFSB in AMI may be ascribed to its ability to regulate arachidonic acid metabolism.展开更多
Non-alcoholic fatty liver disease(NAFLD)has emerged as a predominant contributor to chronic liver disorders and hepatocellular carcinoma,representing a significant global health burden.Mounting evidence has implicated...Non-alcoholic fatty liver disease(NAFLD)has emerged as a predominant contributor to chronic liver disorders and hepatocellular carcinoma,representing a significant global health burden.Mounting evidence has implicated obesity,metabolic syndrome,oxidative stress,and insulin resistance(IR)as central drivers of NAFLD pathogenesis.Despite the absence of approved pharmacological therapies or clearly defined molecular targets,flavonoids have garnered increasing attention for their therapeutic potential.As key bioactive molecules,flavonoids may modulate critical gene networks implicated in the progression of NAFLD.In addition to mitigating lipid accumulation induced by IR,these compounds exert multifaceted regulatory effects by modulating proteasome activity,intracellular signaling cascades,gene transcription,and inflammatory processes that contribute to hepatic lipid dysregulation.展开更多
The objective of this study was to predict,screen,synthesize,and investigate cocrystals of poorly soluble flavonoids that are commonly found in dietary supplements with bipolar compound picolinic acid(PA).To improve t...The objective of this study was to predict,screen,synthesize,and investigate cocrystals of poorly soluble flavonoids that are commonly found in dietary supplements with bipolar compound picolinic acid(PA).To improve the efficiency and success rate of experimental screening,two virtual tools based on hydrogen bond propensity(HBP)and modified molecular electrostatic potential(MEP)maps were used.The prediction accuracy of HBP and MEP is 58.82%and 94.11%,respectively,presenting that the MEP model is very powerful in the discovery of pharmaceutical cocrystals.Among the 12 successfully obtained cocrystals,4 single crystals of PA with luteolin(LUT),genistein(GEN),taxifolin(TAX),dihydromyricetin(DHM)were obtained for the first time.Charged-assisted O-H…O and N-H…O hydrogen bonds appear as main hydrogen bonding synthons,and PA adopts a zwitterionic form after cocrystallization.GEN-PA,TAX-PA,and DHM-PA showed higher DPPH'radical-scavenging capacities;LUT-PA and DHM-PA showed higher ABTS^(+)radical-scavenging capacities;GEN-PA and DHM-PA possessed better protective effects on H9c2 cells from hypoxic injury caused by CoCl_(2)than corresponding pure flavonoids.展开更多
Advanced glycation end products(AGEs)are complex compounds formed through interaction of carbonyl groups from saccharides with amino groups in amino acids,proteins,lipids and nucleic acids,mainly via Maillard reaction...Advanced glycation end products(AGEs)are complex compounds formed through interaction of carbonyl groups from saccharides with amino groups in amino acids,proteins,lipids and nucleic acids,mainly via Maillard reaction.Studies have shown that AGEs can accumulate in the body and lead to neurodegenerative diseases,cardiovascular diseases,inflammatory responses,diabetes,and other diseases.This comment will provide a review of the inhibitory mechanism of flavonoids on dietary AGEs formation in food models and aims to provide a theoretical basis for the development of new therapeutic strategies and drugs.展开更多
Sand rice(Agriophyllum squarrosum),a pioneering annual plant thriving in deserts and sandy regions throughout the Asian interior,is believed to be a potential food and forage crop due to its significant nutritional an...Sand rice(Agriophyllum squarrosum),a pioneering annual plant thriving in deserts and sandy regions throughout the Asian interior,is believed to be a potential food and forage crop due to its significant nutritional and medicinal values.Previous metabolomics analyses have revealed that sand rice contains abundant flavonoid components,which are known for their wide applications in cosmetics,food,and pharmaceuticals.To optimize the use of flavonoids in sand rice,in this study,the response surface methodology(RSM)was selected to determine the optimal ultrasonic-assisted extraction(UAE)criteria for flavonoids extraction from the aerial part of sand rice firstly.Statistical analyses unveiled the optimum parameters for flavonoids extraction fromsand rice could be 62%of ethanol concentration,1:43 solid-toliquid ratio,160Wfor ultrasound power,and 52℃for extraction temperature with extraction time of 12 min.Under this condition,the experiment optimumtotal flavonoid yield could reach at 15.24 mg/g,which was correspond to the maximum predicted value of RSM with 15.22 mg/g.Subsequently,the antifungal efficacy of these extracts was evaluated against three common plant pathogenic fungi,showing a significant inhibitory effect with the highest rate of inhibition reaching 25.3%at a concentration of 4 mg/mL,underscoring its potential as a natural antimicrobial agent.This studywill not only provide a powerful method to extract flavonoids froma desert resource plant,but also pave the way for industrial development and application of the promising desert plants with high nutritional and medicinal values.展开更多
Three highly oxidized hybrid flavonoids neosophoflavonoids A–C(1,2a,and 2b)were isolated from the roots of Sophora flavescens.Neosophoflavonoid A possesses a unique highly oxidized heptacyclic6/6/6/6/6/6/5 system.Neo...Three highly oxidized hybrid flavonoids neosophoflavonoids A–C(1,2a,and 2b)were isolated from the roots of Sophora flavescens.Neosophoflavonoid A possesses a unique highly oxidized heptacyclic6/6/6/6/6/6/5 system.Neosophoflavonoids B and C are isomers and share the same highly oxidized hexacyclic 6/6/6/6/6/6 systems.Their planar structures were elucidated from 1D/2D nuclear magnetic resonance(NMR),ultraviolet spectroscopy(UV),infrared spectroscopy(IR),and high resolution electrospray ionization mass spectroscopy(HRESIMS)data.Their absolute configurations were determined by thorough GIAO13C NMR(DP4+)calculation protocol and electronic circular dichroism(ECD)calculation method.The plausible biosynthetic routes for the compounds were also proposed.All compounds exhibited significant protein tyrosine phosphatase-1B(PTP1B)inhibitory activity with half maximal inhibitory concentration(IC_(5)0)values 3.94±0.01,0.38±0.13,and 0.70±0.01μmol/L,respectively.In addition,compared to a positive control fenofibrate(Feno)at 20μmol/L,compounds 2a and 2b exhibited stronger inhibitory effects on lipid accumulation in the oleic acid(OA)-induced cell model at 5 and 10μmol/L.展开更多
Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible actio...Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible action mechanisms of D.offi cinale flowers’flavonoid enriched extract(DOFF)were assessed against gastric ulcer.The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34%of the total extractive was flavonoid,and ultra-high performance liquid chromatography time of flight mass spectrometer(UPLC-QTOF/MS)revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids.In addition,in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions,with drop-in ulcer index from 64.33±8.76 to 32.00±4.47.Similar results were revealed in human gastric mucosal epithelia cells,with cells viability to increase from 27.2%to 61.6%post DOFF administration.To analyzed the protect effect of DOFF,we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways.The results showed that DOFF(320μg/mL)could increase the level of oxidation marker protein(HO-1),apoptosis regulatory protein(Bcl-2)and autophagy marker(LC3β)by 50.84%,43.85%,and 59.21%compared with ethanol-treated group respectively.Further analyzed of the mitochondrial activity and apoptosis pathway,we found that DOFF appeared to mitigate against ethanolinduced gastric mucosal injury via AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.展开更多
OBJECTIVE: To evaluate the analgesic effects of total flavonoids of Longxuejie(Resina Dracaenae Cochinchinensis)(TFDB) and explore the possible analgesic mechanism associated with transient receptor potential vanilloi...OBJECTIVE: To evaluate the analgesic effects of total flavonoids of Longxuejie(Resina Dracaenae Cochinchinensis)(TFDB) and explore the possible analgesic mechanism associated with transient receptor potential vanilloid 1(TRPV1).METHODS: Whole-cell patch clamp technique was used to observe the effects of TFDB on capsaicin-induced TRPV1 currents. Rat experiments in vivo were used to observe the analgesic effects of TFDB. Western blot and immunofluorescence experiments were used to test the change of TRPV1 expression in DRG neurons induced by TFDB.RESULTS: Results showed that TFDB inhibited capsaicin-induced TRPV1 receptor currents in acutely isolated dorsal root ganglion(DRG) neurons of rats and the half inhibitory concentration was(16.7 ± 1.6) mg/L.TFDB(2-20 mg/kg) showed analgesic activity in the phase Ⅱ of formalin test and(0.02-2 mg per paw)reduced capsaicin-induced licking times of rats. TFDB(20 mg/kg) was fully efficacious on complete Freund's adjuvant(CFA)-induced inflammatory thermal hyperalgesia and capsaicin could weaken the analgesic effects. The level of TRPV1 expressions of DRG neurons was also decreased in TFDB-treated CFA-inflammatory pain rats.CONCLUSION: All these results indicated that the analgesic effect of TFDB may contribute to their modulations on both function and expression of TRPV1 channels in DRG neurons.展开更多
Diabetes mellitus remains a major global health issue,and great attention is directed at natural therapeutics.This systematic review aimed to assess the potential of flavonoids as antidiabetic agents by investigating ...Diabetes mellitus remains a major global health issue,and great attention is directed at natural therapeutics.This systematic review aimed to assess the potential of flavonoids as antidiabetic agents by investigating their inhibitory effects onα-glucosidase andα-amylase,two key enzymes involved in starch digestion.Six scientific databases(PubMed,Virtual Health Library,EMBASE,SCOPUS,Web of Science,and WHO Global Index Medicus)were searched until August 21,2022,for in vitro studies reporting IC50 values of purified flavonoids onα-amylase andα-glucosidase,along with corresponding data for acarbose as a positive control.A total of 339 eligible articles were analyzed,resulting in the retrieval of 1643 flavonoid structures.These structures were rigorously standardized and curated,yielding 974 unique compounds,among which 177 flavonoids exhibited inhibition of bothα-glucosidase andα-amylase are presented.Quality assessment utilizing a modified CONSORT checklist and structure-activity relationship(SAR)analysis were performed,revealing crucial features for the simultaneous inhibition of flavonoids against both enzymes.Moreover,the review also addressed several limitations in the current research landscape and proposed potential solutions.The curated datasets are available online at https://github.com/MedChemUMP/FDIGA.展开更多
[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experimen...[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from P.chinense Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried out.The optimized extraction process was adopted to compare the contents of total flavonoids from different parts of P.chinense Pursh.[Results]The best extraction process was extracting the powder of P.chinense Pursh for 2.0 h with 20 times of 55%ethanol by reflux twice.Under this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of P.chinense Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from P.chinense Pursh.The contents of total flavonoids from different parts of P.chinense Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of P.chinense Pursh.展开更多
BACKGROUND Flavonoids,the main class of polyphenols,exhibit antioxidant and antihypertensive properties.AIM To prospectively investigate the impact of flavonoids on arterial stiffness in patients with chronic kidney d...BACKGROUND Flavonoids,the main class of polyphenols,exhibit antioxidant and antihypertensive properties.AIM To prospectively investigate the impact of flavonoids on arterial stiffness in patients with chronic kidney disease(CKD)stagesⅠ-Ⅳ.METHODS In this prospective,single-arm study,CKD patients with arterial hypertension and diabetes mellitus were enrolled.Baseline demographic,clinical,and laboratory variables were recorded.Patients received daily treatment with a phenol-rich dietary supplement for 3 months.Blood pressure,arterial stiffness(carotidfemoral pulse wave velocity,central pulse pressure),and oxidative stress markers(protein carbonyls,total phenolic compound,total antioxidant capacity)were measured at baseline and at study end.RESULTS Sixteen patients(mean age:62.5 years,87.5%male)completed the study.Following intervention,peripheral systolic blood pressure decreased significantly by 14 mmHg(P<0.001).Carotid-femoral pulse wave velocity decreased from 8.9 m/s(baseline)to 8.2 m/s(study end)(P<0.001),and central pulse pressure improved from 59 mmHg to 48 mmHg(P=0.003).Flavonoids also reduced oxidative stress markers including protein carbonyls(P<0.001),total phenolic compound(P=0.001),and total antioxidant capacity(P=0.013).CONCLUSION Flavonoid supplementation in CKD patients shows promise in improving blood pressure,arterial stiffness,and oxidative stress markers.展开更多
基金Supported by Key Research and Development and Achievement Transformation Plan Project of Inner Mongolia Autonomous Region(2023YFHH0093).
文摘[Objectives]Based on spectrophotometry,a method for determining the total flavonoid content in milk powder was established by optimizing sample pretreatment conditions,and method validation was performed.[Methods]Total flavonoids in milk powder were extracted with ethanol using 45℃ water bath ultrasonication for 60 min.Flavonoids contain a benzopyran ring structure and react with aluminum nitrate in a potassium acetate environment to form a yellow complex.The absorbance of this complex was measured at 420 nm for quantitative determination.[Results]The method exhibited a good linear relationship within the range of 0.2-1.0 mg.The limit of detection(LOD)was 0.05 g/100 g,and the limit of quantification(LOQ)was 0.3 g/100 g.When the spiked amount of total flavonoids was in the range of 0.3% to 1.0%,the spike recovery rates were 99.3% to 103.4%,and the relative standard deviations(RSD s)were less than 3.0%.[Conclusions]This method offers advantages such as rapidness,accuracy,good stability,and high sensitivity,and can be used for the detection of total flavonoid content in milk powder.
基金supported by the Shandong Provincial Traditional Chinese Medicine Science and Technology Project“Study on the mechanism of Astragalus polysaccharide inhibiting the occurrence of liver fibrosis through PD-1 regulating NK cell function(2020Q004)”Shandong Provincial Medical and Health Science and Technology Development Project“Mechanism of STAT3 inhibition of liver fibrosis by regulating PD-1+NK cells(202002070991)”Natural Science Foundation of Shandong Province“Astragalus polysaccharides inhibit liver fibrosis by regulating CD49a+NK cells(ZR2022QH111).”。
文摘Chronic obstructive pulmonary disease(COPD)has been a major global public health issue due to its high prevalence,disability,and mortality rates.The pathogenesis of COPD is complex and remains incompletely understood,compounded by a lack of specific and effective clinical therapies.Key pathophysiological mechanisms include oxidative stress,inflammation,programmed cell death,and fibrosis,influenced by external risk factors such as cigarette smoke and internal factors like immune deficiency.Natural flavonoids emerge as promising adjuvant treatments or potential drug candidates for COPD,attributed to their multi-target properties and low toxicity.This article provides an overview of various types and sources of natural flavonoids that exhibit therapeutic effects on COPD,their specific pharmacological actions and detailed mechanisms of action.This review aims to serve as a reference for adjuvant treatment strategies in daily dietary practices and to inspire novel drug candidates for COPD.
基金funded by the grant for Evaluation of Functional Property,Processing and Utilization of Xinjiang Characteristic Plant Resources(G2023046003L)the Xinjiang Uygur Autonomous Region Outstanding Youth Science Fund project(2024D01E11)+1 种基金National Nature Science Fund of China(82060788)Hebei Natural Science Foundation(C2022204167).
文摘Coreopsis tinctoria Nutt.,an edible flowering plant,belongs to the Chrysanthemum family and is mainly grown at high altitudes in Northwestern China.It is rich in polyphenolic compounds,particularly marein and flavomarein,and possesses multiple health-promoting properties,such as antioxidant,hypoglycemic and vasorelaxant effects.Previous bioactivity investigations majorly focused on C.tinctoria and its crude extract.The aim of the present study was to prepare marein-dominant C.tinctoria flavonoids(CF),further investigate the CF protective effects of liver fibrosis induced by carbon tetrachloride and elucidate the associated molecular mechanisms.Results have demonstrated that CF effectively attenuated hepatofibrogenesis by increasing the activity of glutathione(GSH)and superoxide dismutase(SOD);suppressing the hepatic stellate cell(HSC)activation,inhibiting transforming growth factorβ(TGF-β)activation and the production ofα-smooth muscle actin(α-SMA),alleviating the phosphorylation of extracellular signal-regulated kinases1/2(ERK1/2)and small mothers against decapentaplegic1/2(Smad1/2),thus maintaining the collagen metabolic homeostasis in the liver.Our study revealed that CF possesses an efficacious protective effect against chronic hepatic fibrosis due to their strong inhibitory effects of oxidative stress and chronic inflammation.
基金Fundação de AmparoàPesquisa do Estado de São Paulo(FAPESP,Brazil,#2020/11667-0)and Universidade Federal do ABC(UFABC,Brazil)were recipients of fellowships from FAPESP:THLV(#2021/11969-9 and#2024/00828-3),GBS(#2021/14227-3),and GMB(#2024/10858-7)+1 种基金recipients of fellowships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(CAPES,Brazil):MIM(Finance Code 001,#88887.597402/2021-00)recipients of fellowships from Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq,Brazil.):GKD(#145164/2024-1),and DRA(#308819/2022-5).
文摘The intricate landscape of neurodegenerative diseases complicates the search for effective therapeutic approaches.Photoreceptor degeneration,the common endpoint in various retinal diseases,including retinitis pigmentosa and age-related macular degeneration,leads to vision loss or blindness.While primary cell death is driven by genetic mutations,oxidative stress,and neuroinflammation,additional mechanisms contribute to disease progression.In retinitis pigmentosa,a multitude of genetic alterations can trigger the degeneration of photoreceptors,while other retinopathies,such as agerelated macular degeneration,are initiated by combinations of environmental factors,such as diet,smoking,and hypertension,with genetic predispositions.Nutraceutical therapies,which blend the principles of nutrition and pharmaceuticals,aim to harness the health benefits of bioactive compounds for therapeutic applications.These compounds generally possess multi-target effects.Polyphenols and flavonoids,secondary plant metabolites abundant in plant-based foods,are known for their antioxidant,neuroprotective,and anti-inflammatory properties.This review focuses on the potential of polyphenols and flavonoids as nutraceuticals to treat neurodegenerative diseases such as retinitis pigmentosa.Furthermore,the importance of developing reliable delivery methods to enhance the bioavailability and therapeutic efficacy of these compounds will be discussed.By combining nutraceuticals with other emerging therapies,such as genetic and cell-based treatments,it is possible to offer a more comprehensive approach to treating retinal degenerative diseases.These advancements could lead to a viable and accessible option,improving the quality of life for patients with retinal diseases.
基金Supported by Shanghai Putuo District R&D Platform Project(2024QX04).
文摘[Objectives]To explore the effects of fermentation process on the content and functions of flavonoids in Gardenia jasminoides.[Methods]G.jasminoides was fermented by microorganisms,and the fermentation process of total flavonoids from G.jasminoides was optimized,and the antioxidant activity and hyaluronidase inhibitory activity of the fermentation broth were tested.[Results]The best strain for fermentation of total flavonoids in G.jasminoides was Bacillus subtilis.The optimum fermentation conditions were as follows:the solid-liquid ratio was 1:30,the inoculation amount was 2%,and the fermentation time was 24 h.Under these fermentation conditions,the content of total flavonoids in G.jasminoides reached 36.90 mg/g,which was 45.22%higher than that of the control group without microbial fermentation,and it had good DPPH free radical and hydroxyl free radical scavenging ability,and the inhibition ability of hyaluronidase after fermentation was also improved.[Conclusions]This study provides a technical reference for the comprehensive application of G.jasminoides.
基金supported by grants from the National Natural Science Foundation of China(31560459)Jiangxi Provincial Natural Science Foundation(20224ACB205014)The Double Thousands Talents Plan of Jiangxi(jxsq2018102075,jxsq2018102076)。
文摘Inflammatory bowel disease(IBD)is a chronic gastrointestinal disease with a high incidence.Treatment for IBD includes medications and diet,and common anti-inflammatory medications have limitations like drug resistance and serious adverse effects.Accumulating evidence has demonstrated that dietary flavonoids exhibit an alleviative effect on IBD by influencing gut microbiota.The microbiota-derived metabolites also regulate IBD and maintain intestinal homeostasis.In this review,we investigate the therapeutic effect of gut microbiota and metabolites on IBD by intestinal immune and intestinal barrier function.We demonstrate the underlying mechanism of dietary flavonoids as an anti-inflammatory molecule alleviating IBD by regulating gut microbiota,short chain fatty acid(SCFA),bile acid(BA),tryptophan(Trp)metabolism and lipopolysaccharides(LPS)-toll-like receptor 4(TLR4)signaling pathway.Based on structural differences of flavonoids,we summarize the recent research progress on the role of different dietary flavonoids in alleviating IBD by gut microbiota and metabolites in animal and clinical trials.This review indicates that dietary flavonoids targeting gut microbiota and metabolites provide a promising strategy for the treatment of inflammation and novel insights into the management of IBD.
基金Supported by Natural Science Foundation of Sichuan Province(2023NS-FSC0610)the Special Fund of Natural Science for the Central Universities of Southwest Minzu University(ZYN2023071).
文摘[Objectives]To analyze the chemical components of Meconopsis integrifolia(Maxim.)Franch total flavonoids(MITF).[Methods]The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS.The column was performed using ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)with 0.1%formic acid water(A)-acetonitrile(B)with gradient elution at 30℃,injection volume of 2μL,and flow rate of 0.3 mL/min.Electrospray ion source adopted positive and negative ion detection mode with scanning range m/z 100-1000.[Results]A total of 93 compounds were identified from MITF,including 57 flavonoids,25 phenolic acids,9 alkaloids and 2 others.Among them,dimethoxytaxifolin was identified as a possible new compound by SciFinder search,and 67 compounds were first identified in M.integrifolia.[Conclusions]This study provides a scientific foundation for clarifying the material basis of the efficacy of M.integrifolia and improving the quality standards.
基金supported by National Natural Science Foundation of China(No.82374333,No.82173961)LiaoNing Revitalization Talents Program(XLYC2203200)+5 种基金SPU Excellent Youth Program(YQ202310)SPU Youth Career Development plan(ZQN202211)Key Laboratory of polysaccharide bioactivity evaluation of TCM of Liaoning Province-Liaoning Distinguished Professor Project for Ying Jia(2017)High-level innovation and entrepreneurship team of Liaoning Province(XLYC2008029)Liaoning Provincial Department of Education Fund(LJ212410163006,LJ212410163018)Postgraduate Education and Teaching Reform Research Project of Liaoning Province in 2024(LNYJG2024251).
文摘This study aims to explore the mechanism by which flavonoids in Crataegus pinnatifida fruit improve Alzheimer’s disease(AD)through network pharmacology and molecular docking technology.The flavonoid components present in Crataegus pinnatifida fruit were gathered from the HERB,HIT,and ETCM databases,and were further supplemented by relevant published literature.The PubChem and SwissTargetPrediction databases were utilized to predict potential targets,and a“Crataegus pinnatifida fruit-active ingredient-target”network was constructed using Cytoscape 3.9.0 software.The GeneCards database was utilized to identify targets associated with AD,which were subsequently intersected with the active targets of Crataegus pinnatifi da fruit.A protein-protein interaction(PPI)network was constructed using the STRING platform.KEGG enrichment analysis of the core targets was conducted on an online bioinformatics mapping platform,while molecular docking of the primary active components and core targets was executed using AutoDock software.Eight flavonoids and 160 potential targets were identifi ed from Crataegus pinnatifi da fruit,of which 147 targets were linked to AD.The results of the“Crataegus pinnatifi da fruit-active ingredient-target”network indicated that quercetin was the principal flavonoid active ingredient.PPI analysis revealed that SRC and EGFR were the key targets,and KEGG analysis identifi ed the main enrichment pathways as Pathways in cancer,PI3K/Akt signaling pathway,and Proteoglycans in cancer.Molecular docking confi rmed the strong binding affi nity between the core targets and the primary active ingredient.The interaction of quercetin with the key targets SRC and EGFR may represent a signifi cant mechanism by which flavonoids from Crataegus pinnatifi da fruit contribute to the improvement of AD.
文摘Vitex negundo(V.negundo),an Indian herb with a rich historical background for the handling of various complaints,is a member of the Verbenaceae family and is characterized by small trees with pale gray bark.This herb is widely used and has been recognized in traditional medicine for its pharmacological effects on a wide range of diseases.All sections of the plant,but particularly the leaves,contain a variety of secondary metabolites,including alkaloids,phenols,flavonoids,glycoside iridoids,tannins,and terpenes.The system is included in a number of store-bought herbal preparations and has the potential to function as an efficient bio-committee.Cancer continues to be a major cause of death and morbidity in spite of the intervention.One of the leading causes of death is cancer,and current therapies can have unpleasant side effects.Unhindered reactions,resistance to traditional cancer medications,radiation therapy,chemotherapy,and restricted access to tumor tissue are some of the reasons why treatment frequently fails.To reduce side effects,increase chemotherapy sensitivity,and slow the spread of cancer,new approaches are required.Small food molecules have been suggested in numerous research as supplemental therapies for cancer patients.The effectiveness of the flavonoid-rich V.negundo extract in treating cancer was assessed mechanically in this investigation.Total flavonoids have been isolated for qualitative phytochemical investigation using V.negundo ethanolic extract.This article highlights significant developments in this field and explores how flavonoids contribute to the targeted suppression of the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin pathway in different cancer types.
基金sponsored by Shandong Provincial Key Research and Development Program(Major Technological Innovation Project)([2021]CXGC010508)Guizhou Province Youth Science and Technology Talent Plan(YQK[2023]038)+1 种基金Science and Technology Department of Zunyi City of Guizhou province of China([2020]7)Key project at central government level:the ability establishment of sustainable use for valuable Chinese medicine resources(2060302).
文摘Background:The study aimed to investigate the protective effect and mechanism of total flavonoids of Scutellaria baicalensis(TFSB)on acute myocardial ischemia(AMI)rats by using functional metabonomics.Methods:Rats were divided into the Control,Model,AMI positive control(Propranolol hydrochloride,30 mg/kg),low dose TFSB(50 mg/kg),and high dose TFSB(100 mg/kg)groups.Rats received the corresponding treatment by intragastric administration once daily for 10 consecutive days.Electrocardiogram,myocardial enzyme,triphenyltetrazolium chloride staining,hematoxylin-eosin,and enzyme-linked immunosorbent assay were performed to evaluate the protective effect of TFSB on AMI rats.Then,the UHPLC-Q-Orbitrap MS method based on serum metabolomics was utilised to search for metabolic biomarkers and metabolic pathways.Subsequently,Western blot and RT-PCR techniques were employed to identify the respective genes and proteins.Results:Pharmacodynamics revealed that TFSB could ameliorate AMI in rats.The results of the metabolomics analysis indicated that the alterations in metabolic profile observed in rats with AMI were partially improved by treatment with TFSB.Moreover,the mRNA expression levels of 5-lipoxygenase(5-LOX)and 15-lipoxygenase(15-LOX)and the protein expression levels of 5-LOX,15-LOX,interleukin-1β(IL-1β),and NF-κB p65 were reduced following treatment with TFSB.Conclusion:The potential treatment of TFSB in AMI may be ascribed to its ability to regulate arachidonic acid metabolism.
基金Chongqing Municipal High-Level Medical Talent Development Project for Middle-Aged and Young ProfessionalsChongqing Health Commission of Traditional Chinese Medicine Research Project(Grant No.2025WSJK175)。
文摘Non-alcoholic fatty liver disease(NAFLD)has emerged as a predominant contributor to chronic liver disorders and hepatocellular carcinoma,representing a significant global health burden.Mounting evidence has implicated obesity,metabolic syndrome,oxidative stress,and insulin resistance(IR)as central drivers of NAFLD pathogenesis.Despite the absence of approved pharmacological therapies or clearly defined molecular targets,flavonoids have garnered increasing attention for their therapeutic potential.As key bioactive molecules,flavonoids may modulate critical gene networks implicated in the progression of NAFLD.In addition to mitigating lipid accumulation induced by IR,these compounds exert multifaceted regulatory effects by modulating proteasome activity,intracellular signaling cascades,gene transcription,and inflammatory processes that contribute to hepatic lipid dysregulation.
基金supported by the Beijing Natural Science Foundation(No.7222261)CAMS Innovation Fund for Medical Sciences(No.2022-I2M-1-015)。
文摘The objective of this study was to predict,screen,synthesize,and investigate cocrystals of poorly soluble flavonoids that are commonly found in dietary supplements with bipolar compound picolinic acid(PA).To improve the efficiency and success rate of experimental screening,two virtual tools based on hydrogen bond propensity(HBP)and modified molecular electrostatic potential(MEP)maps were used.The prediction accuracy of HBP and MEP is 58.82%and 94.11%,respectively,presenting that the MEP model is very powerful in the discovery of pharmaceutical cocrystals.Among the 12 successfully obtained cocrystals,4 single crystals of PA with luteolin(LUT),genistein(GEN),taxifolin(TAX),dihydromyricetin(DHM)were obtained for the first time.Charged-assisted O-H…O and N-H…O hydrogen bonds appear as main hydrogen bonding synthons,and PA adopts a zwitterionic form after cocrystallization.GEN-PA,TAX-PA,and DHM-PA showed higher DPPH'radical-scavenging capacities;LUT-PA and DHM-PA showed higher ABTS^(+)radical-scavenging capacities;GEN-PA and DHM-PA possessed better protective effects on H9c2 cells from hypoxic injury caused by CoCl_(2)than corresponding pure flavonoids.
基金financially supported by the National Natural Science Foundation of China(Grant No.32472468 and No.32001817)the Fundamental Research Program of Shanxi Province(202303021221123).
文摘Advanced glycation end products(AGEs)are complex compounds formed through interaction of carbonyl groups from saccharides with amino groups in amino acids,proteins,lipids and nucleic acids,mainly via Maillard reaction.Studies have shown that AGEs can accumulate in the body and lead to neurodegenerative diseases,cardiovascular diseases,inflammatory responses,diabetes,and other diseases.This comment will provide a review of the inhibitory mechanism of flavonoids on dietary AGEs formation in food models and aims to provide a theoretical basis for the development of new therapeutic strategies and drugs.
基金support received from the Lanzhou Youth Science and Technology Talent Innovation Project(Grant No.:2023-QN-140)National Natural Science Foundation of China(Grant Nos.:31901079,32171608,32271695 and 32201378)the Chinese Academy of Sciences Strategic Biological Resources Program(Grant No.:KFJ-BRP-007-015).
文摘Sand rice(Agriophyllum squarrosum),a pioneering annual plant thriving in deserts and sandy regions throughout the Asian interior,is believed to be a potential food and forage crop due to its significant nutritional and medicinal values.Previous metabolomics analyses have revealed that sand rice contains abundant flavonoid components,which are known for their wide applications in cosmetics,food,and pharmaceuticals.To optimize the use of flavonoids in sand rice,in this study,the response surface methodology(RSM)was selected to determine the optimal ultrasonic-assisted extraction(UAE)criteria for flavonoids extraction from the aerial part of sand rice firstly.Statistical analyses unveiled the optimum parameters for flavonoids extraction fromsand rice could be 62%of ethanol concentration,1:43 solid-toliquid ratio,160Wfor ultrasound power,and 52℃for extraction temperature with extraction time of 12 min.Under this condition,the experiment optimumtotal flavonoid yield could reach at 15.24 mg/g,which was correspond to the maximum predicted value of RSM with 15.22 mg/g.Subsequently,the antifungal efficacy of these extracts was evaluated against three common plant pathogenic fungi,showing a significant inhibitory effect with the highest rate of inhibition reaching 25.3%at a concentration of 4 mg/mL,underscoring its potential as a natural antimicrobial agent.This studywill not only provide a powerful method to extract flavonoids froma desert resource plant,but also pave the way for industrial development and application of the promising desert plants with high nutritional and medicinal values.
基金supported by the National Natural Science Foundation of China(No.81973194)Biomedical High Performance Computing Platform,Chinese Academy of Medical Sciences。
文摘Three highly oxidized hybrid flavonoids neosophoflavonoids A–C(1,2a,and 2b)were isolated from the roots of Sophora flavescens.Neosophoflavonoid A possesses a unique highly oxidized heptacyclic6/6/6/6/6/6/5 system.Neosophoflavonoids B and C are isomers and share the same highly oxidized hexacyclic 6/6/6/6/6/6 systems.Their planar structures were elucidated from 1D/2D nuclear magnetic resonance(NMR),ultraviolet spectroscopy(UV),infrared spectroscopy(IR),and high resolution electrospray ionization mass spectroscopy(HRESIMS)data.Their absolute configurations were determined by thorough GIAO13C NMR(DP4+)calculation protocol and electronic circular dichroism(ECD)calculation method.The plausible biosynthetic routes for the compounds were also proposed.All compounds exhibited significant protein tyrosine phosphatase-1B(PTP1B)inhibitory activity with half maximal inhibitory concentration(IC_(5)0)values 3.94±0.01,0.38±0.13,and 0.70±0.01μmol/L,respectively.In addition,compared to a positive control fenofibrate(Feno)at 20μmol/L,compounds 2a and 2b exhibited stronger inhibitory effects on lipid accumulation in the oleic acid(OA)-induced cell model at 5 and 10μmol/L.
基金supported by National Key Research and Development Program for Intergovernmental International Science and Technology Innovation Cooperation(2024YFE0109500)National Natural Science Foundation of China(32072149).
文摘Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible action mechanisms of D.offi cinale flowers’flavonoid enriched extract(DOFF)were assessed against gastric ulcer.The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34%of the total extractive was flavonoid,and ultra-high performance liquid chromatography time of flight mass spectrometer(UPLC-QTOF/MS)revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids.In addition,in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions,with drop-in ulcer index from 64.33±8.76 to 32.00±4.47.Similar results were revealed in human gastric mucosal epithelia cells,with cells viability to increase from 27.2%to 61.6%post DOFF administration.To analyzed the protect effect of DOFF,we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways.The results showed that DOFF(320μg/mL)could increase the level of oxidation marker protein(HO-1),apoptosis regulatory protein(Bcl-2)and autophagy marker(LC3β)by 50.84%,43.85%,and 59.21%compared with ethanol-treated group respectively.Further analyzed of the mitochondrial activity and apoptosis pathway,we found that DOFF appeared to mitigate against ethanolinduced gastric mucosal injury via AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.
基金High Level Talents Project of Affiliated Hospital of Youjiang Medical University for Nationalities:Study of Soft-Du'an Capsule's Mechanism and Efficacy of Regulating TRPV1 Pashways in Relieving Oral and Maxillofacial Trigeminal Neuralgia (No. YYFYR20213002)Innovative Group Project of Natural Science Foundation of Hubei Province:Study on the Mechanisms of Pain Signal Transduction and Drug Analgesia (No. 2020CFA025)。
文摘OBJECTIVE: To evaluate the analgesic effects of total flavonoids of Longxuejie(Resina Dracaenae Cochinchinensis)(TFDB) and explore the possible analgesic mechanism associated with transient receptor potential vanilloid 1(TRPV1).METHODS: Whole-cell patch clamp technique was used to observe the effects of TFDB on capsaicin-induced TRPV1 currents. Rat experiments in vivo were used to observe the analgesic effects of TFDB. Western blot and immunofluorescence experiments were used to test the change of TRPV1 expression in DRG neurons induced by TFDB.RESULTS: Results showed that TFDB inhibited capsaicin-induced TRPV1 receptor currents in acutely isolated dorsal root ganglion(DRG) neurons of rats and the half inhibitory concentration was(16.7 ± 1.6) mg/L.TFDB(2-20 mg/kg) showed analgesic activity in the phase Ⅱ of formalin test and(0.02-2 mg per paw)reduced capsaicin-induced licking times of rats. TFDB(20 mg/kg) was fully efficacious on complete Freund's adjuvant(CFA)-induced inflammatory thermal hyperalgesia and capsaicin could weaken the analgesic effects. The level of TRPV1 expressions of DRG neurons was also decreased in TFDB-treated CFA-inflammatory pain rats.CONCLUSION: All these results indicated that the analgesic effect of TFDB may contribute to their modulations on both function and expression of TRPV1 channels in DRG neurons.
基金funded by the University of Medicine and Pharmacy at Ho Chi Minh City under grant number 162/2019/HĐ-ĐHYD for Thanh-Dao Tran.
文摘Diabetes mellitus remains a major global health issue,and great attention is directed at natural therapeutics.This systematic review aimed to assess the potential of flavonoids as antidiabetic agents by investigating their inhibitory effects onα-glucosidase andα-amylase,two key enzymes involved in starch digestion.Six scientific databases(PubMed,Virtual Health Library,EMBASE,SCOPUS,Web of Science,and WHO Global Index Medicus)were searched until August 21,2022,for in vitro studies reporting IC50 values of purified flavonoids onα-amylase andα-glucosidase,along with corresponding data for acarbose as a positive control.A total of 339 eligible articles were analyzed,resulting in the retrieval of 1643 flavonoid structures.These structures were rigorously standardized and curated,yielding 974 unique compounds,among which 177 flavonoids exhibited inhibition of bothα-glucosidase andα-amylase are presented.Quality assessment utilizing a modified CONSORT checklist and structure-activity relationship(SAR)analysis were performed,revealing crucial features for the simultaneous inhibition of flavonoids against both enzymes.Moreover,the review also addressed several limitations in the current research landscape and proposed potential solutions.The curated datasets are available online at https://github.com/MedChemUMP/FDIGA.
基金Supported by Key Research and Development Program of Sichuan Province(2022YFS0436)Natural Science Foundation of Sichuan Province(2022NSFSC1738)+4 种基金Science and Technology Planning Project of Luzhou City(2021-JYJ-109,2023SYF120)Special Project of Traditional Chinese Medicine Scientific Research of Sichuan Provincial Administration of Traditional Chinese Medicine(2020CP0029)Southwest Medical University-Luzhou Hospital of Traditional Chinese Medicine Base Project(2019-LH003)Open Subject of Luzhou Key Laboratory of Fine Chemical Application Technology(HYJY-2106-B)Southwest Medical University Undergraduate Student Innovation and Entrepreneurship Training Program(202310632074).
文摘[Objectives]This study was conducted to optimize the extraction process of total flavonoids from Penthorum chinense Pursh and compare their contents from different parts.[Methods]Single factor and orthogonal experiments were designed to optimize the extraction process of total flavonoids from P.chinense Pursh with the volume fraction of ethanol,the ratio of material to liquid,heating reflux extraction time and extraction times as factors,and the content of total flavonoids as the index.A verification test was carried out.The optimized extraction process was adopted to compare the contents of total flavonoids from different parts of P.chinense Pursh.[Results]The best extraction process was extracting the powder of P.chinense Pursh for 2.0 h with 20 times of 55%ethanol by reflux twice.Under this condition,the contents of total flavonoids were 3.63%,8.90%,11.28%,and 4.36%from stems,leaves,flowers and whole grass of P.chinense Pursh,respectively.[Conclusions]The process is reasonable,feasible and stable,and can effectively extract total flavonoids from P.chinense Pursh.The contents of total flavonoids from different parts of P.chinense Pursh were quite different,and the value was higher in the leaves and flowers,so the proportions of leaves and flowers should be paid attention to in the industrial processing of P.chinense Pursh.
基金Supported by the Greek Public Sector and the European Regional Development Fund,No.ΔΜΡ1-0010874 and No.MIS 5068931.
文摘BACKGROUND Flavonoids,the main class of polyphenols,exhibit antioxidant and antihypertensive properties.AIM To prospectively investigate the impact of flavonoids on arterial stiffness in patients with chronic kidney disease(CKD)stagesⅠ-Ⅳ.METHODS In this prospective,single-arm study,CKD patients with arterial hypertension and diabetes mellitus were enrolled.Baseline demographic,clinical,and laboratory variables were recorded.Patients received daily treatment with a phenol-rich dietary supplement for 3 months.Blood pressure,arterial stiffness(carotidfemoral pulse wave velocity,central pulse pressure),and oxidative stress markers(protein carbonyls,total phenolic compound,total antioxidant capacity)were measured at baseline and at study end.RESULTS Sixteen patients(mean age:62.5 years,87.5%male)completed the study.Following intervention,peripheral systolic blood pressure decreased significantly by 14 mmHg(P<0.001).Carotid-femoral pulse wave velocity decreased from 8.9 m/s(baseline)to 8.2 m/s(study end)(P<0.001),and central pulse pressure improved from 59 mmHg to 48 mmHg(P=0.003).Flavonoids also reduced oxidative stress markers including protein carbonyls(P<0.001),total phenolic compound(P=0.001),and total antioxidant capacity(P=0.013).CONCLUSION Flavonoid supplementation in CKD patients shows promise in improving blood pressure,arterial stiffness,and oxidative stress markers.
