FKF1是响应蓝光节律表达的基因,是光周期途径调控植物开花的重要因子之一。为探索BdFKF1基因在光周期途径中调控烟草植株开花的分子机制,该研究以野生型烟草(SR1)和转BdFKF1基因烟草(BdFKF1-OE)植株为材料,利用转录组学测序技术并进行RT...FKF1是响应蓝光节律表达的基因,是光周期途径调控植物开花的重要因子之一。为探索BdFKF1基因在光周期途径中调控烟草植株开花的分子机制,该研究以野生型烟草(SR1)和转BdFKF1基因烟草(BdFKF1-OE)植株为材料,利用转录组学测序技术并进行RT-qPCR验证,观测并记录2种材料的开花时间。结果表明:(1)在SR1 vs FKF1组中,共筛选出472个差异表达基因,包括上调基因248个、下调基因224个,其中与光周期相关的差异表达基因有14个,包括上调基因7个、下调基因7个。(2)GO富集分析发现,差异表达基因大量富集在U5小核核糖核蛋白体和氧化还原酶活性,作用于成对供体和蓝光反应等通路中,其中与光周期相关的差异表达基因主要富集在FK506结合、光周期和开花、光周期、磷脂酰乙醇胺结合、大环内酯结合、花发育调控等通路中。(3)KEGG富集分析发现,差异表达基因主要富集在ABC转运蛋白、内质网中的蛋白质加工、角质,木栓素和蜡的生物合成、昼夜节律-植物等通路中,其中与光周期相关的差异表达基因主要富集在植物激素信号转导、昼夜节律-植物通路中。(4)通过观察记录发现BdFKF1-OE植株开花时间比SR1提前3.9 d。(5)RT-qPCR结果与转录组学数据变化趋势基本一致,表明转录组数据有较高的可靠性。综上认为,在长日照条件下,BdFKF1基因会影响光周期途径相关基因的表达,同时过表达BdFKF1基因具有促进烟草植株开花的作用。展开更多
Plants constantly survey the surrounding environment using several sets of photoreceptors. They can sense changes in the quantity （= intensity） and quality （=wavelength） of light and use this information to adjust...Plants constantly survey the surrounding environment using several sets of photoreceptors. They can sense changes in the quantity （= intensity） and quality （=wavelength） of light and use this information to adjust their physiological responses, growth, and developmental patterns. In addition to the classical photoreceptors, such as phytochromes, crypto- chromes, and phototropins, ZEITLUPE （ZTL）, FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 （FKF1）, and LOV KELCH PROTEIN 2 （LKP2） proteins have been recently identified as blue-light photoreceptors that are important for regulation of the circadian clock and photoperiodic flowering. The ZTL/FKF1/LKP2 protein family possesses a unique combination of domains： a blue-light-absorb- ing LOV （Light, Oxygen, or Voltage） domain along with domains involved in protein degradation. Here, we summarize recent advances in our understanding of the function of the Arabidopsis ZTL/FKF1/LKP2 proteins. We summarize the distinct pho- tochemical properties of their LOV domains and discuss the molecular mechanisms by which the ZTL/FKF1/LKP2 proteins reg- ulate the circadian clock and photoperiodic flowering by controlling blue-light-dependent protein degradation.展开更多
文摘FKF1是响应蓝光节律表达的基因,是光周期途径调控植物开花的重要因子之一。为探索BdFKF1基因在光周期途径中调控烟草植株开花的分子机制,该研究以野生型烟草(SR1)和转BdFKF1基因烟草(BdFKF1-OE)植株为材料,利用转录组学测序技术并进行RT-qPCR验证,观测并记录2种材料的开花时间。结果表明:(1)在SR1 vs FKF1组中,共筛选出472个差异表达基因,包括上调基因248个、下调基因224个,其中与光周期相关的差异表达基因有14个,包括上调基因7个、下调基因7个。(2)GO富集分析发现,差异表达基因大量富集在U5小核核糖核蛋白体和氧化还原酶活性,作用于成对供体和蓝光反应等通路中,其中与光周期相关的差异表达基因主要富集在FK506结合、光周期和开花、光周期、磷脂酰乙醇胺结合、大环内酯结合、花发育调控等通路中。(3)KEGG富集分析发现,差异表达基因主要富集在ABC转运蛋白、内质网中的蛋白质加工、角质,木栓素和蜡的生物合成、昼夜节律-植物等通路中,其中与光周期相关的差异表达基因主要富集在植物激素信号转导、昼夜节律-植物通路中。(4)通过观察记录发现BdFKF1-OE植株开花时间比SR1提前3.9 d。(5)RT-qPCR结果与转录组学数据变化趋势基本一致,表明转录组数据有较高的可靠性。综上认为,在长日照条件下,BdFKF1基因会影响光周期途径相关基因的表达,同时过表达BdFKF1基因具有促进烟草植株开花的作用。
文摘Plants constantly survey the surrounding environment using several sets of photoreceptors. They can sense changes in the quantity （= intensity） and quality （=wavelength） of light and use this information to adjust their physiological responses, growth, and developmental patterns. In addition to the classical photoreceptors, such as phytochromes, crypto- chromes, and phototropins, ZEITLUPE （ZTL）, FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 （FKF1）, and LOV KELCH PROTEIN 2 （LKP2） proteins have been recently identified as blue-light photoreceptors that are important for regulation of the circadian clock and photoperiodic flowering. The ZTL/FKF1/LKP2 protein family possesses a unique combination of domains： a blue-light-absorb- ing LOV （Light, Oxygen, or Voltage） domain along with domains involved in protein degradation. Here, we summarize recent advances in our understanding of the function of the Arabidopsis ZTL/FKF1/LKP2 proteins. We summarize the distinct pho- tochemical properties of their LOV domains and discuss the molecular mechanisms by which the ZTL/FKF1/LKP2 proteins reg- ulate the circadian clock and photoperiodic flowering by controlling blue-light-dependent protein degradation.
