目的观察沙利度胺对二氧化硅所致肺纤维化形成的影响。方法SD大鼠54只,随机分为3组,模型组、沙利度胺治疗组和对照组。模型组和沙利度胺治疗组气管内注入二氧化硅混悬液复制实验室大鼠矽肺模型,对照组在相同条件下给予生理盐水。第二天...目的观察沙利度胺对二氧化硅所致肺纤维化形成的影响。方法SD大鼠54只,随机分为3组,模型组、沙利度胺治疗组和对照组。模型组和沙利度胺治疗组气管内注入二氧化硅混悬液复制实验室大鼠矽肺模型,对照组在相同条件下给予生理盐水。第二天起沙利度胺治疗组给予沙利度胺饲料喂养,其余各组在相同条件下普通饲料喂养。分别在染尘后7、30、60d分批处死大鼠,观察其组织的病理变化,并采用比色法测定肺组织羟脯氨酸含量、免疫组织化学法观察各组大鼠肺组织中FIZZ1(found in inflamm atory zone 1,FIZZ1)蛋白的表达、实时荧光定量PCR法检测FIZZ1mRNA的表达水平。结果沙利度胺治疗组羟脯氨酸含量低于模型组,但高于对照组;FIZZ1蛋白在正常肺组织为弱表达,模型组第7天时表达明显增强,第30天时较第7天时有所减弱,第60天时明显减弱,但强于对照组;沙利度胺治疗组在第7天、第30天及第60天FIZZ1蛋白的表达均较对照组高,但低于模型组,尤其是第7天的表达降低较明显,但第60天降低不甚明显;PCR也显示出同样的结果。结论沙利度胺治疗后减少了矽肺大鼠肺组织中羟脯氨酸的含量,从而抑制胶原形成,对影响矽肺形成有一定的积极作用,但其是否也通过抑制FIZZ1而减轻矽肺的发生尚有待进一步研究。展开更多
FIZZ/RELM is a new gene family named ＂found in inflammatory zone＂ （FIZZ） or ＂re- sistin-like molecule＂ （RELM）. FIZZ1/RELMct is specifically expressed in lung tissue and associated with pulmonary inflammation. ...FIZZ/RELM is a new gene family named ＂found in inflammatory zone＂ （FIZZ） or ＂re- sistin-like molecule＂ （RELM）. FIZZ1/RELMct is specifically expressed in lung tissue and associated with pulmonary inflammation. Chronic cigarette smoking up-regulates FIZZ 1/RELMct expression in rat lung tissues, the mechanism of which is related to cigarette smoking-induced airway hyperresponsive- ness. To investigate the effect of exercise training on chronic cigarette smoking-induced airway hyper- responsiveness and up-regulation of FIZZ1/RELMct, rat chronic cigarette smoking model was estab- lished. The rats were treated with regular exercise training and their airway responsiveness was meas- ured. Hematoxylin and eosin （HE） staining, immunohistochemistry and in situ hybridization of lung tissues were performed to detect the expression of FIZZ1/RELMct. Results revealed that proper exercise training decreased airway hyperresponsiveness and pulmonary inflammation in rat chronic cigarette smoking model. Cigarette smoking increased the mRNA and protein levels of FIZZ1/RELMct, which were reversed by the proper exercise. It is concluded that proper exercise training prevents up-regulation of FIZZ1/RELMct induced by cigarette smoking, which may be involved in the mechanism of proper exercise training modulating airway hyperresponsiveness.展开更多
文摘目的观察沙利度胺对二氧化硅所致肺纤维化形成的影响。方法SD大鼠54只,随机分为3组,模型组、沙利度胺治疗组和对照组。模型组和沙利度胺治疗组气管内注入二氧化硅混悬液复制实验室大鼠矽肺模型,对照组在相同条件下给予生理盐水。第二天起沙利度胺治疗组给予沙利度胺饲料喂养,其余各组在相同条件下普通饲料喂养。分别在染尘后7、30、60d分批处死大鼠,观察其组织的病理变化,并采用比色法测定肺组织羟脯氨酸含量、免疫组织化学法观察各组大鼠肺组织中FIZZ1(found in inflamm atory zone 1,FIZZ1)蛋白的表达、实时荧光定量PCR法检测FIZZ1mRNA的表达水平。结果沙利度胺治疗组羟脯氨酸含量低于模型组,但高于对照组;FIZZ1蛋白在正常肺组织为弱表达,模型组第7天时表达明显增强,第30天时较第7天时有所减弱,第60天时明显减弱,但强于对照组;沙利度胺治疗组在第7天、第30天及第60天FIZZ1蛋白的表达均较对照组高,但低于模型组,尤其是第7天的表达降低较明显,但第60天降低不甚明显;PCR也显示出同样的结果。结论沙利度胺治疗后减少了矽肺大鼠肺组织中羟脯氨酸的含量,从而抑制胶原形成,对影响矽肺形成有一定的积极作用,但其是否也通过抑制FIZZ1而减轻矽肺的发生尚有待进一步研究。
基金supported by grants from the National Natural Science Foundation of China (No. 81200020, 30770943,30770648, and 31271490)
文摘FIZZ/RELM is a new gene family named ＂found in inflammatory zone＂ （FIZZ） or ＂re- sistin-like molecule＂ （RELM）. FIZZ1/RELMct is specifically expressed in lung tissue and associated with pulmonary inflammation. Chronic cigarette smoking up-regulates FIZZ 1/RELMct expression in rat lung tissues, the mechanism of which is related to cigarette smoking-induced airway hyperresponsive- ness. To investigate the effect of exercise training on chronic cigarette smoking-induced airway hyper- responsiveness and up-regulation of FIZZ1/RELMct, rat chronic cigarette smoking model was estab- lished. The rats were treated with regular exercise training and their airway responsiveness was meas- ured. Hematoxylin and eosin （HE） staining, immunohistochemistry and in situ hybridization of lung tissues were performed to detect the expression of FIZZ1/RELMct. Results revealed that proper exercise training decreased airway hyperresponsiveness and pulmonary inflammation in rat chronic cigarette smoking model. Cigarette smoking increased the mRNA and protein levels of FIZZ1/RELMct, which were reversed by the proper exercise. It is concluded that proper exercise training prevents up-regulation of FIZZ1/RELMct induced by cigarette smoking, which may be involved in the mechanism of proper exercise training modulating airway hyperresponsiveness.
