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In Vitro Fertilization in Maize and Tobacco: The Fusion Patterns During Sexual Cell Fusion 被引量:1
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作者 孙蒙祥 杨弘远 Mauro CRESTI 《Acta Botanica Sinica》 CSCD 2002年第10期1142-1145,共4页
Large scale of sexual cell fusion was carried out in order to observe the fusion pattern during in vitro fertilization with special interest in the influence of cell volume on the membrane behavior. Three patterns cou... Large scale of sexual cell fusion was carried out in order to observe the fusion pattern during in vitro fertilization with special interest in the influence of cell volume on the membrane behavior. Three patterns could be recognized in sexual cell fusion, which was supported by fluorescent microscopy with the aid of video enhanced system and cooled CCD. It was found that the fusion pattern and cell membrane behavior were related to the cell volume ratio of two fusing cells. The results reported here might be useful for interpreting and evaluating data from in vitro fertilization experiments and for explaining male cytoplasm exclusion during fertilization. 展开更多
关键词 in vitro fertilization MAIZE TOBACCO cell volume fusion pattern fitc-con A
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