Studying immiscible fluid displacement patterns can provide a better understanding of displacement processes within heterogeneous porous media,thereby helping improving oil recovery and optimizing geological CO_(2) se...Studying immiscible fluid displacement patterns can provide a better understanding of displacement processes within heterogeneous porous media,thereby helping improving oil recovery and optimizing geological CO_(2) sequestration.As the injection rate of water displacing oil increases and the displacement pattern transits from capillary fingering to viscous fingering,there is a broad crossover zone between the two that can adversely affect the oil displacement efficiency.While previous studies have utilized phase diagrams to investigate the influence of the viscosity ratio and wettability of the crossover zone,fewer have studied the impact of rock heterogeneity.In this study,we created pore network models with varying degrees of heterogeneity to simulate water flooding at different injection rates.Our model quantifies capillary and viscous fingering characteristics while investigating porous media heterogeneity's role in the crossover zone.Analysis of simulation results reveals that a higher characteristic front flow rate within the crossover zone leads to earlier breakthrough and reduced displacement efficiency.Increased heterogeneity in the porous media raises injection-site pressure,lowers water saturation,and elevates the characteristic front flow rate,thereby expanding the extent of crossover zone.展开更多
BACKGROUND Colorectal cancer(CRC)is one of the most common causes of cancer mortality worldwide.The transcription factor Myc-associated zinc finger protein(MAZ)has been implicated in cancer progression.However,its pre...BACKGROUND Colorectal cancer(CRC)is one of the most common causes of cancer mortality worldwide.The transcription factor Myc-associated zinc finger protein(MAZ)has been implicated in cancer progression.However,its precise function and mecha-nisms in CRC remain unclear.AIM To investigate the role and mechanism of the MAZ/ubiquitin-like with PHD and RING finger domains 1(UHRF1)/esophageal cancer-related gene 4(ECRG4)axis in CRC metastasis.METHODS Western blot,quantitative reverse transcription polymerase chain reaction(PCR)and transwell were performed to evaluate the impact of MAZ knockdown on CRC cell migration and invasion.A xenograft tumor metastasis model was es-tablished by injecting MAZ-deficient CRC cells into nude mice to assess in vivo metastatic potential.Dual-luciferase reporter assay was performed to determine the role of MAZ and its downstream target,UHRF1.Chromatin immunoprecip-itation-quantitative PCR and methylation-specific PCR were used to analyze whether UHRF1 regulated ECRG4 through DNA methylation.RESULTS MAZ was highly upregulated in CRC cells and promoted CRC migration,inva-sion,epithelial-mesenchymal transition(EMT)and metastasis.Mechanistically,MAZ transcriptionally activated UHRF1,which in turn led to DNA methylation of ECRG4.Knockdown of MAZ suppressed CRC migration and invasion was reversed by overexpression of UHRF1.Loss of UHRF1 upregulated ECRG4,inhibited EMT,and reduced cell migration and invasion.However,simultaneous knockdown of ECRG4 partially reversed these effects.CONCLUSION MAZ promotes CRC cell migration,invasion,and EMT by transcriptionally activating UHRF1,which downreg-ulates ECRG4 through DNA methylation.展开更多
BACKGROUND Hepatocellular carcinoma(HCC)is one of the most prevalent and aggressive forms of liver cancer,with high morbidity and poor prognosis due to late diagnosis and limited treatment options.Despite advances in ...BACKGROUND Hepatocellular carcinoma(HCC)is one of the most prevalent and aggressive forms of liver cancer,with high morbidity and poor prognosis due to late diagnosis and limited treatment options.Despite advances in understanding its molecular mechanisms,effective biomarkers for early detection and targeted therapy remain scarce.Zinc finger protein 71(ZNF71),a zinc-finger protein,has been implicated in various cancers,yet its role in HCC remains largely unexplored.This gap in knowledge underscores the need for further investigation into the ZNF71 of potential as a diagnostic or therapeutic target in HCC.AIM To explore the expression levels,clinical relevance,and molecular mechanisms of ZNF71 in the progression of HCC.METHODS The study evaluated ZNF71 expression in 235 HCC specimens and 13 noncancerous liver tissue samples using immunohistochemistry.High-throughput datasets were employed to assess the differential expression of ZNF71 in HCC and its association with clinical and pathological features.The impact of ZNF71 on HCC cell line growth was examined through clustered regularly interspaced short palindromic repeat knockout screens.Co-expressed genes were identified and analyzed for enrichment using LinkedOmics and Sangerbox 3.0,focusing on significant correlations(P<0.01,correlation coefficient≥0.3).Furthermore,the relationship between ZNF71 expression and immune cell infiltration was quantified using TIMER2.0.RESULTS ZNF71 showed higher expression in HCC tissues vs non-tumorous tissues,with a significant statistical difference(P<0.05).Data from the UALCAN platform indicated increased ZNF71 levels across early to mid-stage HCC,correlating with disease severity(P<0.05).High-throughput analysis presented a standardized mean difference in ZNF71 expression of 0.55(95%confidence interval[CI]:0.34-0.75).The efficiency of ZNF71 mRNA was evaluated,yielding an area under the curve of 0.78(95%CI:0.75-0.82),a sensitivity of 0.63(95%CI:0.53-0.72),and a specificity of 0.82(95%CI:0.73-0.89).Diagnostic likelihood ratios were positive at 3.61(95%CI:2.41-5.41)and negative at 0.45(95%CI:0.36-0.56).LinkedOmics analysis identified strong positive correlations of ZNF71 with genes such as ZNF470,ZNF256,and ZNF285.Pathway enrichment analyses highlighted associations with herpes simplex virus type 1 infection,the cell cycle,and DNA replication.Negative correlations involved metabolic pathways,peroxisomes,and fatty acid degradation.TIMER2.0 analysis demonstrated positive correlations of high ZNF71 expression with various immune cell types,including CD4^(+)T cells,B cells,regulatory T cells,monocytes,macrophages,and myeloid dendritic cells.CONCLUSION ZNF71 is significantly upregulated in HCC,correlating with the disease’s clinical and pathological stages.It appears to promote HCC progression through mechanisms involving the cell cycle and metabolism and is associated with immune cell infiltration.These findings suggest that ZNF71 could be a novel target for diagnosing and treating HCC.展开更多
Wheat(Triticum aestivum L.)is a highly valued cereal crop produced and consumed globally,particularly in arid or semi-arid regions(Zhou et al.,2020;Mao et al.,2023).However,its production is increasingly threatened by...Wheat(Triticum aestivum L.)is a highly valued cereal crop produced and consumed globally,particularly in arid or semi-arid regions(Zhou et al.,2020;Mao et al.,2023).However,its production is increasingly threatened by the rising incidence of drought events associated with climate change.Arid regions are especially susceptible to these droughts,which are intensifying in both severity and frequency(Eckardt et al.,2023;Mao et al.,2023;Yang and Qin,2023).As of a 2022 report,more than 92%of wheat-producing regions are estimated to experience one or more drought/heatwave events in each growing season.Furthermore,the duration and frequency of these combined stress events have increased by approximately 28%over the past four decades(He et al.,2022).To address this challenge,wheat breeding programs have allocated substantial and research efforts to developing elite,stress tolerant lines.This initiative is large part by rapid innovation in transgenic and genome editing strategies(Hu and Xiong,2014;Gao et al.,2021.展开更多
Finger reimplantation is a pivotal technique in microsurgery,playing an irreplaceable role in restoring the functional integrity of fingers,maintaining the aesthetic appearance of the hand,ensuring the patient’s abil...Finger reimplantation is a pivotal technique in microsurgery,playing an irreplaceable role in restoring the functional integrity of fingers,maintaining the aesthetic appearance of the hand,ensuring the patient’s ability to perform delicate hand operations,and enhancing their social participation and quality of life.This paper reports on the perioperative nursing approach employed in a successful case of replantation involving a single hand with a multi-planar,20-segment amputation of four fingers.By establishing a specialized nursing team,conducting a comprehensive assessment of the patient’s condition,formulating a personalized nursing plan,and implementing precise nursing care,we achieved successful outcomes.After nearly four weeks of meticulous diagnosis,treatment,and nursing,17 segments of the replanted fingers survived,preserving the functional length of the amputated fingers and maximizing the restoration of hand function.This nursing method provides valuable insights and approaches for effectively managing vascular crises following digital replantation,improving the replantation survival rate,and enhancing patient satisfaction.展开更多
[Objectives]To explore the effects of motivational interviewing intervention based on the transtheoretical model(TTM)on psychological resilience and self-management in patients undergoing finger reimplantation after a...[Objectives]To explore the effects of motivational interviewing intervention based on the transtheoretical model(TTM)on psychological resilience and self-management in patients undergoing finger reimplantation after amputation.[Methods]The patients with finger replantation due to fractures admitted from October 2024 to June 2025 were divided into either the control group or the observation group according to the random number table method,with 40 cases in each group.The control group received conventional perioperative care,while the observation group underwent motivational interviewing based on TTM framework on the basis of the control group.The psychological resilience and self-management levels of the two patient groups were then compared following their respective care interventions.[Results]The psychological resilience and self-management scores of the patients were significantly higher than those of the control group after the intervention,with the difference being statistically significant(P<0.05).[Conclusions]Motivational interviewing based on the TTM can effectively improve the psychological resilience and self-management in patients undergoing severed finger reimplantation,while effectively reducing the occurrence of vascular crisis.展开更多
Background With the increasing prominence of hand and finger motion tracking in virtual reality(VR)applications and rehabilitation studies,data gloves have emerged as a prevalent solution.In this study,we developed an...Background With the increasing prominence of hand and finger motion tracking in virtual reality(VR)applications and rehabilitation studies,data gloves have emerged as a prevalent solution.In this study,we developed an innovative,lightweight,and detachable data glove tailored for finger motion tracking in VR environments.Methods The glove design incorporates a potentiometer coupled with a flexible rack and pinion gear system,facilitating precise and natural hand gestures for interaction with VR applications.Initially,we calibrated the potentiometer to align with the actual finger bending angle,and verified the accuracy of angle measurements recorded by the data glove.To verify the precision and reliability of our data glove,we conducted repeatability testing for flexion(grip test)and extension(flat test),with 250 measurements each,across five users.We employed the Gage Repeatability and Reproducibility to analyze and interpret the repeatable data.Furthermore,we integrated the gloves into a SteamVR home environment using the OpenGlove auto-calibration tool.Conclusions The repeatability analysis revealed an aggregate error of 1.45 degrees in both the gripped and flat hand positions.This outcome was notably favorable when compared with the findings from assessments of nine alternative data gloves that employed similar protocols.In these experiments,users navigated and engaged with virtual objects,underlining the glove's exact tracking of finger motion.Furthermore,the proposed data glove exhibited a low response time of 17-34 ms and back-drive force of only 0.19 N.Additionally,according to a comfort evaluation using the Comfort Rating Scales,the proposed glove system is wearable,placing it at the WL1 level.展开更多
Following the publication of Xu et al.(2022),an error was identified in Figure 1D.Specifically,the top left panel was inadvertently duplicated during figure preparation.To ensure the accuracy and integrity of our publ...Following the publication of Xu et al.(2022),an error was identified in Figure 1D.Specifically,the top left panel was inadvertently duplicated during figure preparation.To ensure the accuracy and integrity of our published work,we request the publication of a corrigendum with the corrected image.We apologize for this oversight and any confusion it may have caused.The amended figure is provided in the updated Supplementary Materials.展开更多
A recent study by Qin et al emphasized the potential of zinc finger protein 71(ZNF71)as a promising biomarker for hepatocellular carcinoma(HCC).The authors offered valuable insights into the relationship between ZNF71...A recent study by Qin et al emphasized the potential of zinc finger protein 71(ZNF71)as a promising biomarker for hepatocellular carcinoma(HCC).The authors offered valuable insights into the relationship between ZNF71 and various clinical and pathological stages of HCC.However,several limitations are required to be addressed to improve the findings.These limitations include concerns regarding patient selection,the generalizability of the results,and the necessity for functional validation to establish ZNF71’s specific role in the progression of HCC.Furthermore,statistical issues related to multiple comparisons,confounding variables,and the inherent heterogeneity of high-throughput datasets warrant careful consideration.Future research should focus on multi-institutional cohorts,utilize in vivo models,and compare ZNF71 with established biomarkers to strengthen the clinical relevance of ZNF71.展开更多
蛋白质泛素化修饰广泛参与植物的生长发育及逆境胁迫响应,其中RING-finger型E3泛素连接酶基因salt and drought induced ring finger1(SDIR1)在植物抗逆中具有重要的作用.为了解茶树SDIR1(CsSDIR1)在抗逆应答中的作用机制,采用RT-PCR技...蛋白质泛素化修饰广泛参与植物的生长发育及逆境胁迫响应,其中RING-finger型E3泛素连接酶基因salt and drought induced ring finger1(SDIR1)在植物抗逆中具有重要的作用.为了解茶树SDIR1(CsSDIR1)在抗逆应答中的作用机制,采用RT-PCR技术从茶树中克隆CsSDIR1的全长cDNA序列及启动子序列,对其生物信息学特征进行分析,并采用qRT-PCR技术检测该基因的组织表达特异性及在不同逆境胁迫下的表达模式.结果显示,CsSDIR1基因的开放阅读框(ORF)长831 bp,编码276个氨基酸,蛋白质分子量(Mr)为30.085×103,理论等电点为6.54;氨基酸序列分析表明,CsSDIR1属于疏水性蛋白、定位在胞内膜上,与其他植物中的SDIR1相似性较高,在其N-端和C-端分别含有2个保守的跨膜结构域和C3H2C3 RING finger功能域;CsSDIR1与猕猴桃关系最近. CsSDIR1上游启动子含多个与干旱胁迫和盐胁迫响应相关的元件.表达分析显示,CsSDIR1在茎中的表达量显著高于根、叶和花;ABA、干旱和高盐诱导其表达,低温抑制CsSDIR1的表达.根据上述结果推测CsSDIR1基因可能参与了茶树的抗逆响应.展开更多
The effects of rice transplanters＇ transplanting settings（plant spacing and finger sticking area） on rice yield were investigated in two early-season rice cultivars：hybrid rice cultivar（Zhuliangyou 819） and conv...The effects of rice transplanters＇ transplanting settings（plant spacing and finger sticking area） on rice yield were investigated in two early-season rice cultivars：hybrid rice cultivar（Zhuliangyou 819） and conventional rice cultivar（Zhongjiazao 17）.The results showed that,different transplanting settings resulted in different numbers of transplanted basic seedlings,and the rice yield was significantly correlated with the number of transplanted basic seedlings.The number of transplanted basic seedlings increased with the decrease of plant spacing and the increase of finger sticking area.The rate of lost hills of machine transplanting decreased with the increase of finger sticking area.The leaf area index and amount of dry matter accumulation were highly correlated with the number of transplanted basic seedlings.The average yield of the rice transplanted with a plant spacing of11.5 cm was higher than that with a plant spacing of 13.8 cm.In the field transplanted with the rice under the plant spacing of 13.8 cm,the rice yield increased with the increase of finger sticking area,and the finger sticking area of 2.23 cm2 showed the highest rice yield of 8 174.16 kg/hm2 of Zhuliangyou 819 and 7 925.54kg/hm2 of Zhongjiaozao17.In the field transplanted with the rice under the plant spacing of 11.5 cm,the rice yield was the highest with the finger sticking area of 1.83 cm2,and then decreased with the large finger sticking area.Under the plant spacing of 11.5 cm,the highest rice yields of Zhuliangyou 819 and Zhongjiaozao17 were 8 492.94 kg/hm2 and 8 000.23 kg/hm2,respectively.Therefore,the preferred machine transplanting settings for double-season early rice are a plant spacing of11.5 cm and a finger sticking area of 1.83 cm2,while the finger sticking area should be increased to 2.23 cm2 for conventional rice cultivars.展开更多
Based on flexible pneumatic actuator(FPA),bending joint and side-sway joint,a new kind of pneumatic dexterous robot finger was developed.The finger is equipped with one five-component force sensor and four contactless...Based on flexible pneumatic actuator(FPA),bending joint and side-sway joint,a new kind of pneumatic dexterous robot finger was developed.The finger is equipped with one five-component force sensor and four contactless magnetic rotary encoders.Mechanical parts and FPAs are integrated,which reduces the overall size of the finger.Driven by FPA directly,the joint output torque is more accurate and the friction and vibration can be effectively reduced.An improved adaptive genetic algorithm(IAGA) was adopted to solve the inverse kinematics problem of the redundant finger.The statics of the finger was analyzed and the relation between fingertip force and joint torque was built.Finally,the finger force/position control principle was introduced.Tracking experiments of fingertip force/position were carried out.The experimental results show that the fingertip position tracking error is within ±1 mm and the fingertip force tracking error is within ±0.4 N.It is also concluded from the theoretical and experimental results that the finger can be controlled and it has a good application prospect.展开更多
In this study, we improved an underactuated finger mechanism by using Solidworks to simulate the grasp operation of a finger in some different situations. In addition, a robot palm is designed for the three-finger rob...In this study, we improved an underactuated finger mechanism by using Solidworks to simulate the grasp operation of a finger in some different situations. In addition, a robot palm is designed for the three-finger robot hand with the designed underactuated fingers. A Solidworks simulation was used to verify the rationality of the design. Some parts of the hand were modified to fit for 3D printing, and a prototype of the hand was produced by 3D printing, which could reduce the cost of the production process, as well as provide design flexibility and other advantages. Finally, some grasping experiments were made with the prototype. The results showed that the robot could grasp objects with different sizes, and further verified the rationality of the design and feasibility of fabricating the robot hand using 3D printing.展开更多
BACKGROUND： The detection of motor evoked potential is utilized to explore neuromuscular finger coordination. The influence of transcranial magnetic stimulation on finger force has been investigated mainly on a singl...BACKGROUND： The detection of motor evoked potential is utilized to explore neuromuscular finger coordination. The influence of transcranial magnetic stimulation on finger force has been investigated mainly on a single finger, and only time-dependent increased target finger force has been detected in the finger force task. OBJECTIVE： To explore the neural mechanism of finger force coordination in the motor cortex by observing the influence of various finger coordination patterns and patterns of transcranial magnetic stimulation （TMS）-induced finger force changes. DESIGN, TIME AND SETTING： Neurophysiological and behavioral study was performed at the Biomedical Engineering Laboratory of Chongqing University from April to June 2008. PARTICIPANTS： A total of 10 healthy, university students, comprising 5 males and 5 females, aged 21-23 years, voluntarily participated in this study. All participants were right-handed, with normal or corrected vision. Individuals with upper limb complaints or other musculoskeletal disorders were excluded. METHODS： A target force-tracking task was conducted on the index finger, the index and middle fingers, and four fingers （index, middle, ring, and little）, respectively. Target force trace in a single trial consisted of a 6-second ramp phase, a 20-second constant phase, and a 6-second drop phase. During experimentation, an unpredictable single-pulse TMS （120% motor threshold） was applied to the primary motor cortex （M1） in each phase. MAIN OUTCOME MEASURES： Changes in peak force induced by TMS were obtained for each finger pattern during each force-tracking phase. Differences in force changes were tested between different finger pattems with regard to ramp, constant, and drop phases of target force. RESULTS： Under ramp, constant, and drop phases of target force, the increase in magnetic stimulation-induced finger forces changes positively correlated with the number of fingers involved in the force tracking task. The magnetic stimulation-induced force changes from the index finger were less than the combination of the index and middle fingers or all four fingers under the corresponding target force, and the force changes from the combination of the index and middle fingers were less than all four fingers, Le., index finger 〈 index and middle fingers 〈 four fingers. CONCLUSION： Different neuromuscular mechanisms could be involved in finger force production for different finger combination patterns. Results from the present study suggested that independent motor neurons regulated individual finger force production.展开更多
基金supported by the Research and Innovation Fund for Graduate Students of Southwest Petroleum University(No.2022KYCX027)supported by the National Natural Science Foundation for Youth Grant(No.41902157).
文摘Studying immiscible fluid displacement patterns can provide a better understanding of displacement processes within heterogeneous porous media,thereby helping improving oil recovery and optimizing geological CO_(2) sequestration.As the injection rate of water displacing oil increases and the displacement pattern transits from capillary fingering to viscous fingering,there is a broad crossover zone between the two that can adversely affect the oil displacement efficiency.While previous studies have utilized phase diagrams to investigate the influence of the viscosity ratio and wettability of the crossover zone,fewer have studied the impact of rock heterogeneity.In this study,we created pore network models with varying degrees of heterogeneity to simulate water flooding at different injection rates.Our model quantifies capillary and viscous fingering characteristics while investigating porous media heterogeneity's role in the crossover zone.Analysis of simulation results reveals that a higher characteristic front flow rate within the crossover zone leads to earlier breakthrough and reduced displacement efficiency.Increased heterogeneity in the porous media raises injection-site pressure,lowers water saturation,and elevates the characteristic front flow rate,thereby expanding the extent of crossover zone.
基金Supported by Hangzhou Medical and Health Science and Technology Plan,No.B20210014.
文摘BACKGROUND Colorectal cancer(CRC)is one of the most common causes of cancer mortality worldwide.The transcription factor Myc-associated zinc finger protein(MAZ)has been implicated in cancer progression.However,its precise function and mecha-nisms in CRC remain unclear.AIM To investigate the role and mechanism of the MAZ/ubiquitin-like with PHD and RING finger domains 1(UHRF1)/esophageal cancer-related gene 4(ECRG4)axis in CRC metastasis.METHODS Western blot,quantitative reverse transcription polymerase chain reaction(PCR)and transwell were performed to evaluate the impact of MAZ knockdown on CRC cell migration and invasion.A xenograft tumor metastasis model was es-tablished by injecting MAZ-deficient CRC cells into nude mice to assess in vivo metastatic potential.Dual-luciferase reporter assay was performed to determine the role of MAZ and its downstream target,UHRF1.Chromatin immunoprecip-itation-quantitative PCR and methylation-specific PCR were used to analyze whether UHRF1 regulated ECRG4 through DNA methylation.RESULTS MAZ was highly upregulated in CRC cells and promoted CRC migration,inva-sion,epithelial-mesenchymal transition(EMT)and metastasis.Mechanistically,MAZ transcriptionally activated UHRF1,which in turn led to DNA methylation of ECRG4.Knockdown of MAZ suppressed CRC migration and invasion was reversed by overexpression of UHRF1.Loss of UHRF1 upregulated ECRG4,inhibited EMT,and reduced cell migration and invasion.However,simultaneous knockdown of ECRG4 partially reversed these effects.CONCLUSION MAZ promotes CRC cell migration,invasion,and EMT by transcriptionally activating UHRF1,which downreg-ulates ECRG4 through DNA methylation.
基金Supported by Joint Project on Regional High Incidence Diseases Research of Guangxi Natural Science Foundation,No.2024GXNSFAA010057 and No.2024GXNSFAA010085Natural Science Foundation of Guangxi,China,No.2022GXNSFBA035657+2 种基金Guangxi Zhuang Autonomous Region Health Commission Self-Financed Scientific Research Project,No.Z20210764Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine Scientific Research Project,No.GXZYA20230270 and No.GXZYA20240305Advanced Innovation Teams and Xinghu Scholars Program of Guangxi Medical University(2022).
文摘BACKGROUND Hepatocellular carcinoma(HCC)is one of the most prevalent and aggressive forms of liver cancer,with high morbidity and poor prognosis due to late diagnosis and limited treatment options.Despite advances in understanding its molecular mechanisms,effective biomarkers for early detection and targeted therapy remain scarce.Zinc finger protein 71(ZNF71),a zinc-finger protein,has been implicated in various cancers,yet its role in HCC remains largely unexplored.This gap in knowledge underscores the need for further investigation into the ZNF71 of potential as a diagnostic or therapeutic target in HCC.AIM To explore the expression levels,clinical relevance,and molecular mechanisms of ZNF71 in the progression of HCC.METHODS The study evaluated ZNF71 expression in 235 HCC specimens and 13 noncancerous liver tissue samples using immunohistochemistry.High-throughput datasets were employed to assess the differential expression of ZNF71 in HCC and its association with clinical and pathological features.The impact of ZNF71 on HCC cell line growth was examined through clustered regularly interspaced short palindromic repeat knockout screens.Co-expressed genes were identified and analyzed for enrichment using LinkedOmics and Sangerbox 3.0,focusing on significant correlations(P<0.01,correlation coefficient≥0.3).Furthermore,the relationship between ZNF71 expression and immune cell infiltration was quantified using TIMER2.0.RESULTS ZNF71 showed higher expression in HCC tissues vs non-tumorous tissues,with a significant statistical difference(P<0.05).Data from the UALCAN platform indicated increased ZNF71 levels across early to mid-stage HCC,correlating with disease severity(P<0.05).High-throughput analysis presented a standardized mean difference in ZNF71 expression of 0.55(95%confidence interval[CI]:0.34-0.75).The efficiency of ZNF71 mRNA was evaluated,yielding an area under the curve of 0.78(95%CI:0.75-0.82),a sensitivity of 0.63(95%CI:0.53-0.72),and a specificity of 0.82(95%CI:0.73-0.89).Diagnostic likelihood ratios were positive at 3.61(95%CI:2.41-5.41)and negative at 0.45(95%CI:0.36-0.56).LinkedOmics analysis identified strong positive correlations of ZNF71 with genes such as ZNF470,ZNF256,and ZNF285.Pathway enrichment analyses highlighted associations with herpes simplex virus type 1 infection,the cell cycle,and DNA replication.Negative correlations involved metabolic pathways,peroxisomes,and fatty acid degradation.TIMER2.0 analysis demonstrated positive correlations of high ZNF71 expression with various immune cell types,including CD4^(+)T cells,B cells,regulatory T cells,monocytes,macrophages,and myeloid dendritic cells.CONCLUSION ZNF71 is significantly upregulated in HCC,correlating with the disease’s clinical and pathological stages.It appears to promote HCC progression through mechanisms involving the cell cycle and metabolism and is associated with immune cell infiltration.These findings suggest that ZNF71 could be a novel target for diagnosing and treating HCC.
基金supported by grants from the Major Project on Agricultural Bio-breeding of China(2023ZD04026)National Natural Science Foundation of China(31872866 and 32372124)+2 种基金China Postdoctoral Science Foundation(2022M721101)National Natural Science Foundation of Hunan(2023JJ40132)Natural Science Foundation of Chongqing(CSTB2023NSCQ-MSX0542).
文摘Wheat(Triticum aestivum L.)is a highly valued cereal crop produced and consumed globally,particularly in arid or semi-arid regions(Zhou et al.,2020;Mao et al.,2023).However,its production is increasingly threatened by the rising incidence of drought events associated with climate change.Arid regions are especially susceptible to these droughts,which are intensifying in both severity and frequency(Eckardt et al.,2023;Mao et al.,2023;Yang and Qin,2023).As of a 2022 report,more than 92%of wheat-producing regions are estimated to experience one or more drought/heatwave events in each growing season.Furthermore,the duration and frequency of these combined stress events have increased by approximately 28%over the past four decades(He et al.,2022).To address this challenge,wheat breeding programs have allocated substantial and research efforts to developing elite,stress tolerant lines.This initiative is large part by rapid innovation in transgenic and genome editing strategies(Hu and Xiong,2014;Gao et al.,2021.
文摘Finger reimplantation is a pivotal technique in microsurgery,playing an irreplaceable role in restoring the functional integrity of fingers,maintaining the aesthetic appearance of the hand,ensuring the patient’s ability to perform delicate hand operations,and enhancing their social participation and quality of life.This paper reports on the perioperative nursing approach employed in a successful case of replantation involving a single hand with a multi-planar,20-segment amputation of four fingers.By establishing a specialized nursing team,conducting a comprehensive assessment of the patient’s condition,formulating a personalized nursing plan,and implementing precise nursing care,we achieved successful outcomes.After nearly four weeks of meticulous diagnosis,treatment,and nursing,17 segments of the replanted fingers survived,preserving the functional length of the amputated fingers and maximizing the restoration of hand function.This nursing method provides valuable insights and approaches for effectively managing vascular crises following digital replantation,improving the replantation survival rate,and enhancing patient satisfaction.
基金Supported by 2023 Shiyan Taihe Hospital Fund Project(2023JJXM024).
文摘[Objectives]To explore the effects of motivational interviewing intervention based on the transtheoretical model(TTM)on psychological resilience and self-management in patients undergoing finger reimplantation after amputation.[Methods]The patients with finger replantation due to fractures admitted from October 2024 to June 2025 were divided into either the control group or the observation group according to the random number table method,with 40 cases in each group.The control group received conventional perioperative care,while the observation group underwent motivational interviewing based on TTM framework on the basis of the control group.The psychological resilience and self-management levels of the two patient groups were then compared following their respective care interventions.[Results]The psychological resilience and self-management scores of the patients were significantly higher than those of the control group after the intervention,with the difference being statistically significant(P<0.05).[Conclusions]Motivational interviewing based on the TTM can effectively improve the psychological resilience and self-management in patients undergoing severed finger reimplantation,while effectively reducing the occurrence of vascular crisis.
基金Supported by the Sirindhorn International Institute of Technology,Thammasat University,EFS-G(Excellent foreign Student-Graduate)research fund.
文摘Background With the increasing prominence of hand and finger motion tracking in virtual reality(VR)applications and rehabilitation studies,data gloves have emerged as a prevalent solution.In this study,we developed an innovative,lightweight,and detachable data glove tailored for finger motion tracking in VR environments.Methods The glove design incorporates a potentiometer coupled with a flexible rack and pinion gear system,facilitating precise and natural hand gestures for interaction with VR applications.Initially,we calibrated the potentiometer to align with the actual finger bending angle,and verified the accuracy of angle measurements recorded by the data glove.To verify the precision and reliability of our data glove,we conducted repeatability testing for flexion(grip test)and extension(flat test),with 250 measurements each,across five users.We employed the Gage Repeatability and Reproducibility to analyze and interpret the repeatable data.Furthermore,we integrated the gloves into a SteamVR home environment using the OpenGlove auto-calibration tool.Conclusions The repeatability analysis revealed an aggregate error of 1.45 degrees in both the gripped and flat hand positions.This outcome was notably favorable when compared with the findings from assessments of nine alternative data gloves that employed similar protocols.In these experiments,users navigated and engaged with virtual objects,underlining the glove's exact tracking of finger motion.Furthermore,the proposed data glove exhibited a low response time of 17-34 ms and back-drive force of only 0.19 N.Additionally,according to a comfort evaluation using the Comfort Rating Scales,the proposed glove system is wearable,placing it at the WL1 level.
文摘Following the publication of Xu et al.(2022),an error was identified in Figure 1D.Specifically,the top left panel was inadvertently duplicated during figure preparation.To ensure the accuracy and integrity of our published work,we request the publication of a corrigendum with the corrected image.We apologize for this oversight and any confusion it may have caused.The amended figure is provided in the updated Supplementary Materials.
文摘A recent study by Qin et al emphasized the potential of zinc finger protein 71(ZNF71)as a promising biomarker for hepatocellular carcinoma(HCC).The authors offered valuable insights into the relationship between ZNF71 and various clinical and pathological stages of HCC.However,several limitations are required to be addressed to improve the findings.These limitations include concerns regarding patient selection,the generalizability of the results,and the necessity for functional validation to establish ZNF71’s specific role in the progression of HCC.Furthermore,statistical issues related to multiple comparisons,confounding variables,and the inherent heterogeneity of high-throughput datasets warrant careful consideration.Future research should focus on multi-institutional cohorts,utilize in vivo models,and compare ZNF71 with established biomarkers to strengthen the clinical relevance of ZNF71.
文摘蛋白质泛素化修饰广泛参与植物的生长发育及逆境胁迫响应,其中RING-finger型E3泛素连接酶基因salt and drought induced ring finger1(SDIR1)在植物抗逆中具有重要的作用.为了解茶树SDIR1(CsSDIR1)在抗逆应答中的作用机制,采用RT-PCR技术从茶树中克隆CsSDIR1的全长cDNA序列及启动子序列,对其生物信息学特征进行分析,并采用qRT-PCR技术检测该基因的组织表达特异性及在不同逆境胁迫下的表达模式.结果显示,CsSDIR1基因的开放阅读框(ORF)长831 bp,编码276个氨基酸,蛋白质分子量(Mr)为30.085×103,理论等电点为6.54;氨基酸序列分析表明,CsSDIR1属于疏水性蛋白、定位在胞内膜上,与其他植物中的SDIR1相似性较高,在其N-端和C-端分别含有2个保守的跨膜结构域和C3H2C3 RING finger功能域;CsSDIR1与猕猴桃关系最近. CsSDIR1上游启动子含多个与干旱胁迫和盐胁迫响应相关的元件.表达分析显示,CsSDIR1在茎中的表达量显著高于根、叶和花;ABA、干旱和高盐诱导其表达,低温抑制CsSDIR1的表达.根据上述结果推测CsSDIR1基因可能参与了茶树的抗逆响应.
文摘The effects of rice transplanters＇ transplanting settings（plant spacing and finger sticking area） on rice yield were investigated in two early-season rice cultivars：hybrid rice cultivar（Zhuliangyou 819） and conventional rice cultivar（Zhongjiazao 17）.The results showed that,different transplanting settings resulted in different numbers of transplanted basic seedlings,and the rice yield was significantly correlated with the number of transplanted basic seedlings.The number of transplanted basic seedlings increased with the decrease of plant spacing and the increase of finger sticking area.The rate of lost hills of machine transplanting decreased with the increase of finger sticking area.The leaf area index and amount of dry matter accumulation were highly correlated with the number of transplanted basic seedlings.The average yield of the rice transplanted with a plant spacing of11.5 cm was higher than that with a plant spacing of 13.8 cm.In the field transplanted with the rice under the plant spacing of 13.8 cm,the rice yield increased with the increase of finger sticking area,and the finger sticking area of 2.23 cm2 showed the highest rice yield of 8 174.16 kg/hm2 of Zhuliangyou 819 and 7 925.54kg/hm2 of Zhongjiaozao17.In the field transplanted with the rice under the plant spacing of 11.5 cm,the rice yield was the highest with the finger sticking area of 1.83 cm2,and then decreased with the large finger sticking area.Under the plant spacing of 11.5 cm,the highest rice yields of Zhuliangyou 819 and Zhongjiaozao17 were 8 492.94 kg/hm2 and 8 000.23 kg/hm2,respectively.Therefore,the preferred machine transplanting settings for double-season early rice are a plant spacing of11.5 cm and a finger sticking area of 1.83 cm2,while the finger sticking area should be increased to 2.23 cm2 for conventional rice cultivars.
基金Project(2009AA04Z209) supported by the National High Technology Research and Development Program of ChinaProject(R1090674) supported by the Natural Science Foundation of Zhejiang Province,ChinaProject(51075363) supported by the National Natural Science Foundation of China
文摘Based on flexible pneumatic actuator(FPA),bending joint and side-sway joint,a new kind of pneumatic dexterous robot finger was developed.The finger is equipped with one five-component force sensor and four contactless magnetic rotary encoders.Mechanical parts and FPAs are integrated,which reduces the overall size of the finger.Driven by FPA directly,the joint output torque is more accurate and the friction and vibration can be effectively reduced.An improved adaptive genetic algorithm(IAGA) was adopted to solve the inverse kinematics problem of the redundant finger.The statics of the finger was analyzed and the relation between fingertip force and joint torque was built.Finally,the finger force/position control principle was introduced.Tracking experiments of fingertip force/position were carried out.The experimental results show that the fingertip position tracking error is within ±1 mm and the fingertip force tracking error is within ±0.4 N.It is also concluded from the theoretical and experimental results that the finger can be controlled and it has a good application prospect.
基金supported by National Natural Science Foundation of China (Nos. 51375504 and 61602539)the Program for New Century Excellent Talents in University
文摘In this study, we improved an underactuated finger mechanism by using Solidworks to simulate the grasp operation of a finger in some different situations. In addition, a robot palm is designed for the three-finger robot hand with the designed underactuated fingers. A Solidworks simulation was used to verify the rationality of the design. Some parts of the hand were modified to fit for 3D printing, and a prototype of the hand was produced by 3D printing, which could reduce the cost of the production process, as well as provide design flexibility and other advantages. Finally, some grasping experiments were made with the prototype. The results showed that the robot could grasp objects with different sizes, and further verified the rationality of the design and feasibility of fabricating the robot hand using 3D printing.
基金the National Natural Science Foundation of China, No. 3077054630970758+1 种基金Chongqing Natural Science Foundation, No. 2006BB2043 2007BB5148
文摘BACKGROUND： The detection of motor evoked potential is utilized to explore neuromuscular finger coordination. The influence of transcranial magnetic stimulation on finger force has been investigated mainly on a single finger, and only time-dependent increased target finger force has been detected in the finger force task. OBJECTIVE： To explore the neural mechanism of finger force coordination in the motor cortex by observing the influence of various finger coordination patterns and patterns of transcranial magnetic stimulation （TMS）-induced finger force changes. DESIGN, TIME AND SETTING： Neurophysiological and behavioral study was performed at the Biomedical Engineering Laboratory of Chongqing University from April to June 2008. PARTICIPANTS： A total of 10 healthy, university students, comprising 5 males and 5 females, aged 21-23 years, voluntarily participated in this study. All participants were right-handed, with normal or corrected vision. Individuals with upper limb complaints or other musculoskeletal disorders were excluded. METHODS： A target force-tracking task was conducted on the index finger, the index and middle fingers, and four fingers （index, middle, ring, and little）, respectively. Target force trace in a single trial consisted of a 6-second ramp phase, a 20-second constant phase, and a 6-second drop phase. During experimentation, an unpredictable single-pulse TMS （120% motor threshold） was applied to the primary motor cortex （M1） in each phase. MAIN OUTCOME MEASURES： Changes in peak force induced by TMS were obtained for each finger pattern during each force-tracking phase. Differences in force changes were tested between different finger pattems with regard to ramp, constant, and drop phases of target force. RESULTS： Under ramp, constant, and drop phases of target force, the increase in magnetic stimulation-induced finger forces changes positively correlated with the number of fingers involved in the force tracking task. The magnetic stimulation-induced force changes from the index finger were less than the combination of the index and middle fingers or all four fingers under the corresponding target force, and the force changes from the combination of the index and middle fingers were less than all four fingers, Le., index finger 〈 index and middle fingers 〈 four fingers. CONCLUSION： Different neuromuscular mechanisms could be involved in finger force production for different finger combination patterns. Results from the present study suggested that independent motor neurons regulated individual finger force production.
