The receptor-like kinase(RLK)FERONIA functions in immunity in Arabidopsis.Here,we systemically screened rice RLK genes encoding FERONIA-like receptor(FLRs)that may be involved in rice-Magnaporthe oryzae interaction.Th...The receptor-like kinase(RLK)FERONIA functions in immunity in Arabidopsis.Here,we systemically screened rice RLK genes encoding FERONIA-like receptor(FLRs)that may be involved in rice-Magnaporthe oryzae interaction.The expression of 16 FLR genes was examined in response to the infection of M.oryzae in different rice varieties.For each FLR gene,at least two independent mutants were generated by CRISPR/Cas9 gene-editing technology in rice variety Zhonghua 11(ZH11).Blast disease assay identified that the mutants of FLR1 and FLR13 showed increased susceptibility,whereas the mutants of FLR2 and FLR11 displayed enhanced resistance.Consistently,the mutant of FLR1 enhanced,but the mutant of FLR2 delayed the M.oryzae infection progress,which might be associated with the altered expression of defense-related genes.Together,these data indicate that at least 4 FLR genes are involved in rice-M.oryzae interaction and thus are potentially valuable in blast disease resistance.展开更多
Regulation of RNA metabolism is crucial for the modulation of gene expression in plants exposed to external stresses.However,the mechanism by which plant membrane receptors transmit external signals to regulate RNA me...Regulation of RNA metabolism is crucial for the modulation of gene expression in plants exposed to external stresses.However,the mechanism by which plant membrane receptors transmit external signals to regulate RNA metabolism has not been determined.In this study,we show that FERONIA-like receptor 2(FLR2)modulates innate immunity in rice by inducing the translation of the immune regulator OsEIL1,thereby promoting resistance to Magnaporthe oryzae.FLR2 interacts with and phosphorylates the mRNA processing factor CONSTITUTIVE EXPRESSER OF PATHOGENESIS-RELATED GENES 5(CPR5),leading to modulation of OsEIL1 poly(A)tail length and translation in response to M.oryzae infection.Like the flr2 loss-of-function mutant,the cpr5 mutant exhibited increased resistance to M.oryzae,induced reactive oxygen species generation,and activated the MAPK cascade.Furthermore,the phospho-dead cpr5 mutant(cpr5mut5A),in which the FLR2 phosphorylation sites are altered,could not rescue the cpr5 phenotype and resulted in longer OsEIL1 poly(A)tails than in the wild type.Conversely,the phospho-mimic cpr5mut5D restored both the wild-type phenotype and OsEIL1 poly(A)tail length in the cpr5 mutant background.These findings suggest that FLR2 perceives external stressors and regulates RNA meta-bolism via CPR5,revealing a novel posttranscriptional regulatory pathway in rice.展开更多
基金supported by the grants from the National Natural Science Foundation of China(31901839 and U19A2033)a grant from Sichuan Agricultural University(1922996003).
文摘The receptor-like kinase(RLK)FERONIA functions in immunity in Arabidopsis.Here,we systemically screened rice RLK genes encoding FERONIA-like receptor(FLRs)that may be involved in rice-Magnaporthe oryzae interaction.The expression of 16 FLR genes was examined in response to the infection of M.oryzae in different rice varieties.For each FLR gene,at least two independent mutants were generated by CRISPR/Cas9 gene-editing technology in rice variety Zhonghua 11(ZH11).Blast disease assay identified that the mutants of FLR1 and FLR13 showed increased susceptibility,whereas the mutants of FLR2 and FLR11 displayed enhanced resistance.Consistently,the mutant of FLR1 enhanced,but the mutant of FLR2 delayed the M.oryzae infection progress,which might be associated with the altered expression of defense-related genes.Together,these data indicate that at least 4 FLR genes are involved in rice-M.oryzae interaction and thus are potentially valuable in blast disease resistance.
基金was supported by grants from the National Key R&D Program of China(no.2023YFD1401100)the National Natural Science Foundation of China(NSFC-32071937)the Natural Science Foundation of Hunan Province(2023JJ30443).
文摘Regulation of RNA metabolism is crucial for the modulation of gene expression in plants exposed to external stresses.However,the mechanism by which plant membrane receptors transmit external signals to regulate RNA metabolism has not been determined.In this study,we show that FERONIA-like receptor 2(FLR2)modulates innate immunity in rice by inducing the translation of the immune regulator OsEIL1,thereby promoting resistance to Magnaporthe oryzae.FLR2 interacts with and phosphorylates the mRNA processing factor CONSTITUTIVE EXPRESSER OF PATHOGENESIS-RELATED GENES 5(CPR5),leading to modulation of OsEIL1 poly(A)tail length and translation in response to M.oryzae infection.Like the flr2 loss-of-function mutant,the cpr5 mutant exhibited increased resistance to M.oryzae,induced reactive oxygen species generation,and activated the MAPK cascade.Furthermore,the phospho-dead cpr5 mutant(cpr5mut5A),in which the FLR2 phosphorylation sites are altered,could not rescue the cpr5 phenotype and resulted in longer OsEIL1 poly(A)tails than in the wild type.Conversely,the phospho-mimic cpr5mut5D restored both the wild-type phenotype and OsEIL1 poly(A)tail length in the cpr5 mutant background.These findings suggest that FLR2 perceives external stressors and regulates RNA meta-bolism via CPR5,revealing a novel posttranscriptional regulatory pathway in rice.
