Taking into account the viscoelasticity of the fenugreek gum, a modified Maxwell model in terms of fractional derivatives is developed. Using this model, it is observed that the fenugreek gums with at least two differ...Taking into account the viscoelasticity of the fenugreek gum, a modified Maxwell model in terms of fractional derivatives is developed. Using this model, it is observed that the fenugreek gums with at least two different concentrations obey the Cox-Merz rule.展开更多
AIM: To investigate the effect of fenugreek lactone(FL) on palmitate(PA)-induced apoptosis and dysfunction in insulin secretion in pancreatic NIT-1 β-cells. METHODS: Cells were cultured in the presence or absence of ...AIM: To investigate the effect of fenugreek lactone(FL) on palmitate(PA)-induced apoptosis and dysfunction in insulin secretion in pancreatic NIT-1 β-cells. METHODS: Cells were cultured in the presence or absence of FL and PA(0.25 mmol/L) for 48 h. Then, lipid droplets in NIT-1 cells were observed by oil red O staining, and the intracellular triglyceride content was measured by colorimetric assay. The insulin content in the supernatant was determined using an insulin radioimmunoassay. Oxidative stress-associated parameters, including total superoxide dismutase, glutathione peroxidase and catalase activity and malondialdehyde levels in the suspensions were also examined. The expression of upstream regulators of oxidative stress, such as protein kinase C-α(PKC-α), phospho-PKC-α and P47 phox, were determined by Western blot analysis and real-time PCR. In addition, apoptosis was evaluated in NIT-1 cells by flow cytometry assays and caspase-3 viability assays.RESULTS: Our results indicated that compared to the control group, PA induced an increase in lipid accumulation and apoptosis and a decrease in insulin secretion in NIT-1 cells. Oxidative stress in NIT-1 cells was activated after 48 h of exposure to PA. However, FL reversed the above changes. These effects were accompanied by the inhibition of PKC-α, phosphoPKC-α and P47 phox expression and the activation of caspase-3. CONCLUSION: FL attenuates PA-induced apoptosis and insulin secretion dysfunction in NIT-1 pancreatic β-cells. The mechanism for this action may be associated with improvements in levels of oxidative stress.展开更多
The use of biopolymers for probiotic microencapsulation has been investigated in this paper.The objectives are to enhance its survival rate,colonic release,and stability of these probiotic cultures in digestive condit...The use of biopolymers for probiotic microencapsulation has been investigated in this paper.The objectives are to enhance its survival rate,colonic release,and stability of these probiotic cultures in digestive condition during storage time.Nine types of biopolymers(alginate-psyllium)blend with different concentration of prebiotic;(inulin or fenugreek)were used as candidate for microencapsulation matrix.One strain of probiotic candidates,namely;Enterococcus durans 39C was used in this study.The microencapsulation of this strain with the respective polymer blend was performed by using a simple extrusion method.All blend of formulations have recorded high encapsulation efficiency at value>98%.The survival rate of viable probiotic cells under simulated digestive conditions was also high with value above 47%as compared to non-microencapsulated cells.These nine gel formulations also displayed the high survival rate of viable probiotic cells during storage time(28 d).Their release occurred after 2 h in colonic condition and sustained until 12th h of incubation period.An increase of prebiotic effect value added was observed in incorporated inulin and fenugreek formulations.In short,this study revealed that a new herbal-based psyllium and fenugreek polymers have suitable potential as a matrix for probiotic microencapsulation.展开更多
Objective: To assess the safety and efficacy of herbal formulation rich in standardized fenugreek seed extract(IND-2) add-on therapy in type 2 diabetes mellitus(T2DM) patients who were on insulin treatment in prospect...Objective: To assess the safety and efficacy of herbal formulation rich in standardized fenugreek seed extract(IND-2) add-on therapy in type 2 diabetes mellitus(T2DM) patients who were on insulin treatment in prospective, single arm, open-label, uncontrolled, multicentre trial.Methods: T2DM patients(n=30) with aged 18-80 years who were stabilized on insulin treatment with fasting blood sugar(FBS) level between 100-140 mg/dL received IND-2 capsules(700 mg, thrice a day) for 16 weeks.The primary endpoints were an assessment of FBS at week 2, 4, 6, 8, 12 and 16.Secondary end-points include post-prandial blood sugar level, glycosylated Hb(HbA1c), reduction in the dose of insulin and number of hypoglycemic attacks, and improvement in lipid profile at various weeks.Safety and adverse events(AEs) were also assessed during the study.Results: Study was completed in twenty T2DM patients, and there was no significant reduction in FBS and post-prandial blood sugar level after addon therapy of IND-2.However, add-on therapy of IND-2 significantly reduced(P<0.01) the HbA1c values, requirements of insulin and hypoglycemic events as compared with baseline.Total cholesterol, high-density lipoproteins-cholesterol, and low-density lipoproteincholesterol levels were significantly increased(P<0.01) after IND-2 add-on therapy.Body weight and safety outcomes did not differ significantly in IND-2 add-on therapy group at week 16.Additionally, add-on therapy of IND-2 did not produce any serious adverse events.Conclusions: The results of present investigation suggest that add-on therapy of IND-2 with insulin in T2DM patients improves glycaemic control through a decrease in levels of HbA1c and number of insulin doses needed per day without an increase in body weight and risk of hypoglycemia.Thus, IND-2 may provide a safe and well-tolerated add-on therapy option for the management of T2DM.展开更多
Background: To study the antidiabetic effects and mechanisms of the fenugreek extracts in streptozotocin(STZ)-induced type 2 diabetic(T2 DM) mice fed a high-fat diet(HFD).Methods: We established C57 BL/6 J mice model ...Background: To study the antidiabetic effects and mechanisms of the fenugreek extracts in streptozotocin(STZ)-induced type 2 diabetic(T2 DM) mice fed a high-fat diet(HFD).Methods: We established C57 BL/6 J mice model of T2 DM using HFD-fed and STZinduced method. Then, the mice were administered with two types of fenugreek extracts(E1, flavonoid and E2, stilbene glycoside) for 4 weeks and the effects on fasting blood glucose(FBG), weight, superoxide dismutase(SOD), catalase(CAT),malondialdehyde(MDA), and pathological indexes were investigated.Results: Administration of fenugreek extracts decreased the FBG level compared with that of the model group. Comparatively, the high-dose E2 decreased the FBG more significantly than the other treatments did. Both extracts showed an obvious antioxidant effect by increasing serum SOD and CAT activities and decreasing the MDA content. Furthermore, the high-dose E1 showed a significant difference(P <.01) compared with the model group in the three investigated indexes.Conclusion: Our study demonstrated that both the flavonoid and stilbene glycoside extracts of fenugreek improved the hyperglycemia in the T2 DM mice model. Moreover, the antidiabetic effects of both extracts might be due to their antioxidant activity in vivo.展开更多
The effect of incorporation of fenugreek seed husk (FSH) in muffins at different levels of 5%, 10% and 15% was investigated. Muffins were prepared with FSH at different levels and batter flow properties were studied. ...The effect of incorporation of fenugreek seed husk (FSH) in muffins at different levels of 5%, 10% and 15% was investigated. Muffins were prepared with FSH at different levels and batter flow properties were studied. The muffin batter viscosity increased from 32,500 to 38,000 cps when the FSH content increased from 0 to 15%. While the muffin vol-umes increased upto 10% addition of FSH and further FSH increase did not result beneficially. Supplementation with FSH resulted in softer crumb texture indicated by the hardness which decreases in hardness 4.20 to 3.19 N. Inclusion of FSH addition in muffins found acceptance by panelists with a rating better than the control. The optimal level of incorporation of FSH flour, based on sensory quality in muffins was found to be 10% and use of Polysorbate-60 at 0.5% level in these muffins further improved the overall quality characteristics. These muffins had double the amount of dietary fiber.展开更多
Soluble fiber-rich fenugreek seeds(Trigonella foenum-graecum)and garlic(Allium sativum)are understood to exert cholesterol-lowering and antioxidant effects.The cardioprotective influence of a combination of fenugreek ...Soluble fiber-rich fenugreek seeds(Trigonella foenum-graecum)and garlic(Allium sativum)are understood to exert cholesterol-lowering and antioxidant effects.The cardioprotective influence of a combination of fenugreek seeds and garlic by their antioxidant influence was evaluated in hypercholesterolemic rats administered isoproterenol.Wistar rats were maintained on high-cholesterol diet for 8 weeks along with dietary interventions of fenugreek(10%),garlic(2%)and their combination.Myocardial infarction was induced with isoproterenol injection.Increased circulatory troponin,disturbed activities of cardiac ATPases,increased serum iron and decreased ceruloplasmin confirmed myocardial infarction.Elevated lipid peroxides accompanied with reduced antioxidant molecules caused by isoproterenol and altered activities of antioxidant enzymes in serum and heart in induced myocardial necrosis were countered by dietary fenugreek,garlic,and fenugreek+garlic.Dietary fenugreek seeds and garlic ameliorated isoproterenol-induced compromised antioxidant status,the cardioprotective effect being higher by the combination of fenugreek seeds and garlic.展开更多
The use of new food grade polysaccharides (mucilage) obtained from Hibiscus esculentus and Trigonella foenum graceum,commonly called Okra and Fenugreek,respectively,as flocculants was described.These polysaccharides w...The use of new food grade polysaccharides (mucilage) obtained from Hibiscus esculentus and Trigonella foenum graceum,commonly called Okra and Fenugreek,respectively,as flocculants was described.These polysaccharides were used for removal of solids (suspended solids (SS) and total dissolved solids (TDS)) and dyes from real textile effluents and aqueous solutions of different class of synthetic dyes.Influences of varying polysaccharide concentration,contact time and pH on removal of pollutant from the textile...展开更多
The effects of cold atmospheric-pressure plasma jet (CAPPJ) were investigated on germination and early seedling growth of fenugreek (Trigonella foenum-graecum L) seeds. A two-electrode argon CAPPJ system with and with...The effects of cold atmospheric-pressure plasma jet (CAPPJ) were investigated on germination and early seedling growth of fenugreek (Trigonella foenum-graecum L) seeds. A two-electrode argon CAPPJ system with and without an additional grounded electrode [accelerating grounded (AG) electrode] was used at different exposure times. After 16 h of observation, the germination rates increased by 4 and 7 times, without and with using an AG electrode, respectively, for 1 min of plasma exposure. An increase in shoot fresh weight was observed, especially at 10 min exposure time. A high dry weight of root and shoot at 1 min-AG exposure time was observed. The root:shoot ratio was lower in plasma-treated seedlings, compared with the control plants. The study found that the O-radical emission line (777.4 nm) enhanced 5 times, due to the presence of an AG electrode, which increased the axial electric field and led to the formation of more streamers. The three stated effects (O-radicals, enhancement of the electric field and streamers) could be the cause for the stimulation of seed germination and seedling growth parameters when using the CAPPJ. The scanning electron microscope images showed the etching of the seed surface layers, which was more pronounced when an AG electrode was applied. The results of the current study indicate that the germination rates increased due to the increase of O-radical concentration and the etching of the seed surfaces.展开更多
Purpose： To evaluate the efficacy and safety of the glycoside fraction of fenugreek （Trigonellafoenum-graecum） seeds （Fenu-FG） on physiologi- cal parameters related to muscle anabolism, androgenic hormones, and b...Purpose： To evaluate the efficacy and safety of the glycoside fraction of fenugreek （Trigonellafoenum-graecum） seeds （Fenu-FG） on physiologi- cal parameters related to muscle anabolism, androgenic hormones, and body fat in healthy male subjects during an 8-week resistance training program using a prospective, randomized, double-blind, placebo controlled design. Methods： Sixty healthy male subjects were randomized to ingest capsules of Fenu-FG （1 capsule of 300 rag, twice per day） or the matching placebo at a 1：1 ratio. The subjects participated in a supervised 4-day per week resistance-training program for 8 weeks. The outcome measurements were recorded at recruitment （baseline） and at the end of the treatment （8 weeks）. The efficacy outcome included serum testosterone （total and free） levels, muscle strength and repetitions to failure, metabolic markers for anabolic activity （serum creatinine and blood urea nitrogen）, and % body fat. The standard safety measurements such as adverse events monitoring, vital signs, hematology, biochemistry, and urinalysis were performed. Results： Fenu-FG supplementation demonstrated significant anabolic and androgenic activity as compared with the placebo. Fenu-FG treated subjects showed significant improvements in body fat without a reduction in muscle strength or repetitions to failure. The Fenu-FG supplemen- tation was found to be safe and well-tolerated. Conclusion： Fenu-FG supplementation showed beneficial effects in male subjects during resistance training without any clinical side effects.展开更多
Fenugreek and carob seed were germinated in the dark for 4 days and 15 days respectively. The two species are rich in vitamins A, B1, B3, B8. Germination promotes the increase in their concentrations. To ensure preser...Fenugreek and carob seed were germinated in the dark for 4 days and 15 days respectively. The two species are rich in vitamins A, B1, B3, B8. Germination promotes the increase in their concentrations. To ensure preservation and increased bioavailability of the vitamins, it is necessary to apply a DIC of 30s to 400 kPa making it possible to support the cellular expansion and the decontamination of germinated seeds. Vitamin A content in germinated carob seed increased by 82.54%, which is not the case for fenugreek. A decrease in vitamin A concentration of 4 μg/100 g of dry matter. DIC increase vitamin B1, B3 and B8 in germinated fenugreek seeds. A slight decrease of B vitamins in germinated carob seed after treatment with DIC has been noticed. This loss can be explained by the low bioavailability caused mainly by the botanical structures of the seeds.展开更多
Background:Stress ulcers are common in mechanically ventilated patients.They are the most common cause of gastrointestinal bleeding and are primarily associated with increased the consumption of nonsteroidal anti-infl...Background:Stress ulcers are common in mechanically ventilated patients.They are the most common cause of gastrointestinal bleeding and are primarily associated with increased the consumption of nonsteroidal anti-inflammatory drugs.Despite advanced treatment,it has a high in-hospital mortality rate.This study assessed the effect of fenugreek(Trigonella foenum-graceum L.)seeds on gastrointestinal bleeding in mechanically ventilated patients.Methods:In this double-blind,randomized controlled clinical trial,60 mechanically ventilated patients hospitalized in intensive care units were equally distributed into intervention and control groups.The intervention group received 3 mg fenugreek seeds powder twice daily with a gavage solution for five days,in addition to routine treatment.The control group received only the gavage solution and routine treatment.The gastrointestinal bleeding and abdominal pain at admission and during the five days were assessed.Data were analyzed using SPSS software V.16,and the significance level was set at 0.05.Results:Gastrointestinal bleeding at admission was similar in both groups.The number of mechanically ventilated patients with gastrointestinal bleeding decreased on the third,fourth,and fifth days,but the difference was insignificant.The Cochran test results showed that the rate of stress-induced gastrointestinal bleeding in the control group increased significantly(P=.04).The decrease in gastrointestinal bleeding in the intervention group was not statistically significant(P=.08).Conclusion:The use of this drug reduces the gastrointestinal side effects of ventilated patient.Despite this,reducing gastrointestinal bleeding and similar mechanical ventilation complications in patients entirely dependent on the treating team is an important achievement.展开更多
The objective of the current work is to investigate the possible beneficial effects of forced feeding of fenugreek to rabbit does on their milk composition, milk yield, anemia screening parameters, immunity and the gr...The objective of the current work is to investigate the possible beneficial effects of forced feeding of fenugreek to rabbit does on their milk composition, milk yield, anemia screening parameters, immunity and the growth performance of bunnies. Twenty white New Zealand female rabbits were randomly assigned into 5 groups: normal control and 4 treated groups (vehicle (molasses), fenugreek powdered seeds + molasses, sprouted fenugreek + molasses, and fenugreek oil + molasses). All treated groups took the given doses orally for two days before parturition and 13 days after parturition for 2 successive pregnancies. Blood samples were collected from each doe on the 13<sup>th</sup> day of lactation for determination of hematological, biochemical and immunological parameters. Milk samples were taken for evaluation of milk composition. All rabbit does and their bunnies were weighed for calculation of the milk yield. The fenugreek seeds and oil administered groups (G<sup>3</sup> & G<sup>5</sup>) recorded the highest percent values of fat, protein, lactose, total solids and solid not fat contents of the rabbits’ milk in both 1st and 2nd lactation periods relative to other tested groups. At the beginning and mid of lactation periods, the milk yields recorded non-significant differences either between tested groups or between 1<sup>st</sup> and 2<sup>nd</sup> pregnancy in the same group. Whereas, at the end of lactation, germinated seeds (G<sup>4</sup>) and oil (G<sup>5</sup>) treated groups recorded significantly higher rates of milk yield relative to control and fenugreek seeds groups. Bunnies’ body weights suckling from dams of group III (G<sup>3</sup>) recorded the highest weights relative to other tested groups in both 1<sup>st</sup> and 2<sup>nd</sup> pregnancy. During the 1<sup>st</sup> lactation period, all experimental groups showed significant reduction of white blood cells, while, G<sup>3</sup> and G<sup>4</sup> recorded significant elevation during 2<sup>nd</sup> lactation period. Rabbits treated with fenugreek seeds powder (G<sup>3</sup>) showed a significant raise of phagocytic activity and phagocytic index. Fenugreek seeds group (G<sup>3</sup>) and germinated one (G<sup>4</sup>) showed significant red blood corpuscles and packed cell volume raise relative to other groups during both 1<sup>st</sup> and 2<sup>nd</sup> lactation periods.展开更多
Adding different percentages 1%, 3% and 5% of fenugreek flour depending on maize flour base to gluten free bread recipe for making tanoor bread was used to improve the tanoor bread quality, in term of sensory properti...Adding different percentages 1%, 3% and 5% of fenugreek flour depending on maize flour base to gluten free bread recipe for making tanoor bread was used to improve the tanoor bread quality, in term of sensory properties like volume, crust, color, symmetry, bake uniformity, texture, grain, aroma and taste. Also some rheological properties like gelatinization temperature and maximum viscosity of dough with bread keeping quality were used. The aim of this work is to produce suitable bread with maintaining the bread quality by selecting the suitable percentage to produce gluten free bread for people who suffering from celiac diseases and its contribution to health benefits. Significant differences were observed by using 5% of fenugreek flour in term of gelatinization temperature, maximum viscosity, breed keeping quality and volume. While using 1% of fenugreek flour significantly improved symmetry value, bread texture, crumb color, aroma and taste.展开更多
Seeds of Fenugreek （Trigonellafoenum-graecum L.）, an international spice commodity, are much consumed in Sudan as a food, for flavoring and as a folk-remedy for several ailments, together with local beliefs in nutra...Seeds of Fenugreek （Trigonellafoenum-graecum L.）, an international spice commodity, are much consumed in Sudan as a food, for flavoring and as a folk-remedy for several ailments, together with local beliefs in nutraceutical benefits. The authors have noted an immense variability in colour and other morphological characters of Fenugreek seeds offered in local Sudanese markets and wondered whether this variability is extended to their chemical composition. Steroidal sapogenins are important chemical constituents of Fenugreek seeds in view of their potential as precursors for the commercial synthesis of steroid drugs and their continually revealed beneficial biological activities. In this study, thirty Sudanese Fenugreek seed accessions collected from different geographical regions in Sudan were analyzed for their 25α-, 25β- and total sapogenin content using a simple and specific infra-red spectroscopic method. The seed accessions exhibited much morphological variability particularly in outer seed coat color and size. Preparative TLC followed by gravimetric analysis showed that steroidal sapogenins, mostly diosgenin and yamogenin, represented more than 70% of the steroids of Fenugreek seeds. Infra-red spectroscopic analysis showed that total 25α-sapogenins （calculated as diosgenin） varied from 0.65% to 1.68%. Total 25β-sapogenin （calculated as yamogenin） varied from 0.38% to 2.03%. The content of total sapogenins （α ＋ β epimeric forms） varied between 1.24% and 3.0% of the oven-dry weight of seeds. β-sapogenins （mostly yamogenin） were dominant over α-sapogenins （diosgenin） in most Sudanese Fenugreek seed accessions.展开更多
Alfalfa and fenugreek sprouts are healthy foods,but they are occasionally contaminated with bacterial pathogens and serve as vehicles for transmitting foodborne illnesses.This study examined the efficacy of ascaroside...Alfalfa and fenugreek sprouts are healthy foods,but they are occasionally contaminated with bacterial pathogens and serve as vehicles for transmitting foodborne illnesses.This study examined the efficacy of ascaroside(ascr)#18 treatment for the control of enterohemorrhagic E.coli(EHEC)growth on sprouts.Commercial alfalfa and fenugreek seeds were decontaminated with 20,000 ppm of NaClO,and residual chlorine was neutralized with Dey-Engley broth.Decontaminated seeds were treated with 1 mM or 1μM ascr#18,a plant immunity modulator,before being dried and mixed with sandy soil inoculated with E.coli F4546 or BAA-2326 at 10^(4)-10^(5)CFU/g.The inoculated seeds were sprouted on 1%water agar at 25◦C for 7 days in the dark.Seed or sprout samples were collected on days 0,1,3,5,and 7 for enumeration of bacterial populations.Data was fit into the general linear model and analyzed using Fisher’s least significant different test of the statistical analysis software.Treatment with ascr#18 significantly(P≤0.05)reduced the cell population of EHEC on sprouts.The mean EHEC populations in the 1 mM or 1μM treatment groups were 3.31 or 1.56 log CFU/g lower compared to the control groups.Besides treatment,sprout seed type and sprouting time were also significant independent variables influencing the growth of EHEC,according to the results of type III error analysis.However,EHEC strain type was not a significant independent variable.The study suggests that ascr#18 could be potentially used to control EHEC contamination and improve the microbial safety of sprouts.展开更多
A novelβ-mannanase gene(ScMan26)from Stachybotrys chlorohalonata was expressed in Komagataella phaffii.ScMan26 displayed 69.1%of identity with theβ-mannanase from Thermothelomyces thermophilus ATCC 42464.The highest...A novelβ-mannanase gene(ScMan26)from Stachybotrys chlorohalonata was expressed in Komagataella phaffii.ScMan26 displayed 69.1%of identity with theβ-mannanase from Thermothelomyces thermophilus ATCC 42464.The highestβ-mannanase yield of 45000 U mL^(-1) with protein concentration of 7.8 mg mL^(-1) was obtained in a 5-L fermenter.ScMan26 was most active at 55℃ and pH 6.5,respectively.ScMan26 efficiently hydrolyzed fenugreek gum(15%,w/v)to yield manno-oligosaccharides.The manno-oligosaccharides promoted the growth of Lactobacillus casei AS 1.62 and Bifidobacteria adolescentis ATCC 15703 by 1.8 and 1.7-fold,respectively,compared to the control.Thus,fenugreek manno-oligosaccharides prepared by ScMan26 may be useful as a prebiotic source.展开更多
Saponins are a widely occurring class of secondary metabolites and are of variety of biological activities.Fenugreek,a saponin-rich traditional Chinese herb,has a long history in treatment of different kinds of disord...Saponins are a widely occurring class of secondary metabolites and are of variety of biological activities.Fenugreek,a saponin-rich traditional Chinese herb,has a long history in treatment of different kinds of disorders.In this study,total saponins of Fenugreek(TSOF)was successfully purified by a membrane-resin combined method(purity>90%)and its antioxidative activity was also tested.DPPH and ABTS tests demonstrated its potent radical scavenging activity with IC_(50) values of 0.163 mg/mL and 0.449 mg/mL,respectively.Furthermore,H_(2)O_(2)-induced H9c2 cells was used to identify the protective effect of TSOF.Results showed that TSOF could significantly reduce intracellular reactive oxygen species(ROS)production and malondialdehyde(MDA)level,and enhance intracellular superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSHPx)activities,thereby increasing cell viability and antagonizing cell damage.Morphological observation and LIVE/DEAD staining also demonstrated protective effect of TSOF on oxidative stress at cellular level.Our study presented a promising method for purification of fenugreek saponins.With determination of antioxidant activity of saponins,theoretical and data support were provided for development of fenugreek relative applications.展开更多
Objective: To evaluate the comparative effects of fenugreek(Trigonel a foenum graecum) seed extract(FSE) alone and in combination with an antidiabetic conventional medicine,glibenclamide(GLB),on the inhibition of in v...Objective: To evaluate the comparative effects of fenugreek(Trigonel a foenum graecum) seed extract(FSE) alone and in combination with an antidiabetic conventional medicine,glibenclamide(GLB),on the inhibition of in vitro lipid peroxidation(LPO) in liver,the major target organ of a drug.Methods: LPO was induced by ferrous sulphate(Fe So4),hydrogen peroxide(H2 O2) and carbon tetrachloride(CCl4) and the effects of test seed extract and/or GLB were evaluated.Results: While Fe So4,H2 O2 and CCl4 markedly enhanced the hepatic LPO,simultaneous administration of FSE reduced it in a concentration dependent manner.However,when both FSE and GLB were added to the incubation mixture,chemical y induced hepatic LPO was further inhibited.The test extract also exhibited high antioxidative activity in 1,1-diphenyl-2-picrylhydrazyl radical and in 2,2'-azino-bis,3-ethylbenzothiazoline-6-sulphonic acid radical scavenging assays.Conclusion: FSE therapy in moderate concentration along with a hypoglycemic drug may prove to be advantageous in ameliorating diabetes mel itus and other diseases that are LPO mediated.展开更多
基金Supported by the National Natural Science Foundation of China(No.29576238).
文摘Taking into account the viscoelasticity of the fenugreek gum, a modified Maxwell model in terms of fractional derivatives is developed. Using this model, it is observed that the fenugreek gums with at least two different concentrations obey the Cox-Merz rule.
基金Supported by The National Natural Science Foundation of ChinaNo.81273683 and No.81473637
文摘AIM: To investigate the effect of fenugreek lactone(FL) on palmitate(PA)-induced apoptosis and dysfunction in insulin secretion in pancreatic NIT-1 β-cells. METHODS: Cells were cultured in the presence or absence of FL and PA(0.25 mmol/L) for 48 h. Then, lipid droplets in NIT-1 cells were observed by oil red O staining, and the intracellular triglyceride content was measured by colorimetric assay. The insulin content in the supernatant was determined using an insulin radioimmunoassay. Oxidative stress-associated parameters, including total superoxide dismutase, glutathione peroxidase and catalase activity and malondialdehyde levels in the suspensions were also examined. The expression of upstream regulators of oxidative stress, such as protein kinase C-α(PKC-α), phospho-PKC-α and P47 phox, were determined by Western blot analysis and real-time PCR. In addition, apoptosis was evaluated in NIT-1 cells by flow cytometry assays and caspase-3 viability assays.RESULTS: Our results indicated that compared to the control group, PA induced an increase in lipid accumulation and apoptosis and a decrease in insulin secretion in NIT-1 cells. Oxidative stress in NIT-1 cells was activated after 48 h of exposure to PA. However, FL reversed the above changes. These effects were accompanied by the inhibition of PKC-α, phosphoPKC-α and P47 phox expression and the activation of caspase-3. CONCLUSION: FL attenuates PA-induced apoptosis and insulin secretion dysfunction in NIT-1 pancreatic β-cells. The mechanism for this action may be associated with improvements in levels of oxidative stress.
基金supports of the University Putra Malaysia are gratefully acknowledged.
文摘The use of biopolymers for probiotic microencapsulation has been investigated in this paper.The objectives are to enhance its survival rate,colonic release,and stability of these probiotic cultures in digestive condition during storage time.Nine types of biopolymers(alginate-psyllium)blend with different concentration of prebiotic;(inulin or fenugreek)were used as candidate for microencapsulation matrix.One strain of probiotic candidates,namely;Enterococcus durans 39C was used in this study.The microencapsulation of this strain with the respective polymer blend was performed by using a simple extrusion method.All blend of formulations have recorded high encapsulation efficiency at value>98%.The survival rate of viable probiotic cells under simulated digestive conditions was also high with value above 47%as compared to non-microencapsulated cells.These nine gel formulations also displayed the high survival rate of viable probiotic cells during storage time(28 d).Their release occurred after 2 h in colonic condition and sustained until 12th h of incubation period.An increase of prebiotic effect value added was observed in incorporated inulin and fenugreek formulations.In short,this study revealed that a new herbal-based psyllium and fenugreek polymers have suitable potential as a matrix for probiotic microencapsulation.
文摘Objective: To assess the safety and efficacy of herbal formulation rich in standardized fenugreek seed extract(IND-2) add-on therapy in type 2 diabetes mellitus(T2DM) patients who were on insulin treatment in prospective, single arm, open-label, uncontrolled, multicentre trial.Methods: T2DM patients(n=30) with aged 18-80 years who were stabilized on insulin treatment with fasting blood sugar(FBS) level between 100-140 mg/dL received IND-2 capsules(700 mg, thrice a day) for 16 weeks.The primary endpoints were an assessment of FBS at week 2, 4, 6, 8, 12 and 16.Secondary end-points include post-prandial blood sugar level, glycosylated Hb(HbA1c), reduction in the dose of insulin and number of hypoglycemic attacks, and improvement in lipid profile at various weeks.Safety and adverse events(AEs) were also assessed during the study.Results: Study was completed in twenty T2DM patients, and there was no significant reduction in FBS and post-prandial blood sugar level after addon therapy of IND-2.However, add-on therapy of IND-2 significantly reduced(P<0.01) the HbA1c values, requirements of insulin and hypoglycemic events as compared with baseline.Total cholesterol, high-density lipoproteins-cholesterol, and low-density lipoproteincholesterol levels were significantly increased(P<0.01) after IND-2 add-on therapy.Body weight and safety outcomes did not differ significantly in IND-2 add-on therapy group at week 16.Additionally, add-on therapy of IND-2 did not produce any serious adverse events.Conclusions: The results of present investigation suggest that add-on therapy of IND-2 with insulin in T2DM patients improves glycaemic control through a decrease in levels of HbA1c and number of insulin doses needed per day without an increase in body weight and risk of hypoglycemia.Thus, IND-2 may provide a safe and well-tolerated add-on therapy option for the management of T2DM.
基金National Natural Science Foundation of China,Grant/Award Number:31470426Open Project of State Key Laboratory of Plateau Ecology and Agriculture,Grant/Award Number:2016-KF-05+2 种基金Qinghai Provincial Science Foundation,Grant/Award Number:2016-ZJ-01,2016-ZJ-929QTaishan Scholar Program of Shandong Province,Grant/Award Number:tshw201502046Shandong Provincial Science Foundation,Grant/Award Number:ZR2017MH024
文摘Background: To study the antidiabetic effects and mechanisms of the fenugreek extracts in streptozotocin(STZ)-induced type 2 diabetic(T2 DM) mice fed a high-fat diet(HFD).Methods: We established C57 BL/6 J mice model of T2 DM using HFD-fed and STZinduced method. Then, the mice were administered with two types of fenugreek extracts(E1, flavonoid and E2, stilbene glycoside) for 4 weeks and the effects on fasting blood glucose(FBG), weight, superoxide dismutase(SOD), catalase(CAT),malondialdehyde(MDA), and pathological indexes were investigated.Results: Administration of fenugreek extracts decreased the FBG level compared with that of the model group. Comparatively, the high-dose E2 decreased the FBG more significantly than the other treatments did. Both extracts showed an obvious antioxidant effect by increasing serum SOD and CAT activities and decreasing the MDA content. Furthermore, the high-dose E1 showed a significant difference(P <.01) compared with the model group in the three investigated indexes.Conclusion: Our study demonstrated that both the flavonoid and stilbene glycoside extracts of fenugreek improved the hyperglycemia in the T2 DM mice model. Moreover, the antidiabetic effects of both extracts might be due to their antioxidant activity in vivo.
文摘The effect of incorporation of fenugreek seed husk (FSH) in muffins at different levels of 5%, 10% and 15% was investigated. Muffins were prepared with FSH at different levels and batter flow properties were studied. The muffin batter viscosity increased from 32,500 to 38,000 cps when the FSH content increased from 0 to 15%. While the muffin vol-umes increased upto 10% addition of FSH and further FSH increase did not result beneficially. Supplementation with FSH resulted in softer crumb texture indicated by the hardness which decreases in hardness 4.20 to 3.19 N. Inclusion of FSH addition in muffins found acceptance by panelists with a rating better than the control. The optimal level of incorporation of FSH flour, based on sensory quality in muffins was found to be 10% and use of Polysorbate-60 at 0.5% level in these muffins further improved the overall quality characteristics. These muffins had double the amount of dietary fiber.
文摘Soluble fiber-rich fenugreek seeds(Trigonella foenum-graecum)and garlic(Allium sativum)are understood to exert cholesterol-lowering and antioxidant effects.The cardioprotective influence of a combination of fenugreek seeds and garlic by their antioxidant influence was evaluated in hypercholesterolemic rats administered isoproterenol.Wistar rats were maintained on high-cholesterol diet for 8 weeks along with dietary interventions of fenugreek(10%),garlic(2%)and their combination.Myocardial infarction was induced with isoproterenol injection.Increased circulatory troponin,disturbed activities of cardiac ATPases,increased serum iron and decreased ceruloplasmin confirmed myocardial infarction.Elevated lipid peroxides accompanied with reduced antioxidant molecules caused by isoproterenol and altered activities of antioxidant enzymes in serum and heart in induced myocardial necrosis were countered by dietary fenugreek,garlic,and fenugreek+garlic.Dietary fenugreek seeds and garlic ameliorated isoproterenol-induced compromised antioxidant status,the cardioprotective effect being higher by the combination of fenugreek seeds and garlic.
文摘The use of new food grade polysaccharides (mucilage) obtained from Hibiscus esculentus and Trigonella foenum graceum,commonly called Okra and Fenugreek,respectively,as flocculants was described.These polysaccharides were used for removal of solids (suspended solids (SS) and total dissolved solids (TDS)) and dyes from real textile effluents and aqueous solutions of different class of synthetic dyes.Influences of varying polysaccharide concentration,contact time and pH on removal of pollutant from the textile...
文摘The effects of cold atmospheric-pressure plasma jet (CAPPJ) were investigated on germination and early seedling growth of fenugreek (Trigonella foenum-graecum L) seeds. A two-electrode argon CAPPJ system with and without an additional grounded electrode [accelerating grounded (AG) electrode] was used at different exposure times. After 16 h of observation, the germination rates increased by 4 and 7 times, without and with using an AG electrode, respectively, for 1 min of plasma exposure. An increase in shoot fresh weight was observed, especially at 10 min exposure time. A high dry weight of root and shoot at 1 min-AG exposure time was observed. The root:shoot ratio was lower in plasma-treated seedlings, compared with the control plants. The study found that the O-radical emission line (777.4 nm) enhanced 5 times, due to the presence of an AG electrode, which increased the axial electric field and led to the formation of more streamers. The three stated effects (O-radicals, enhancement of the electric field and streamers) could be the cause for the stimulation of seed germination and seedling growth parameters when using the CAPPJ. The scanning electron microscope images showed the etching of the seed surface layers, which was more pronounced when an AG electrode was applied. The results of the current study indicate that the germination rates increased due to the increase of O-radical concentration and the etching of the seed surfaces.
基金supported by the Indus Biotech Private Ltd.,Pune with no role in the collection,analysis,and interpretation of data and the writing of the report
文摘Purpose： To evaluate the efficacy and safety of the glycoside fraction of fenugreek （Trigonellafoenum-graecum） seeds （Fenu-FG） on physiologi- cal parameters related to muscle anabolism, androgenic hormones, and body fat in healthy male subjects during an 8-week resistance training program using a prospective, randomized, double-blind, placebo controlled design. Methods： Sixty healthy male subjects were randomized to ingest capsules of Fenu-FG （1 capsule of 300 rag, twice per day） or the matching placebo at a 1：1 ratio. The subjects participated in a supervised 4-day per week resistance-training program for 8 weeks. The outcome measurements were recorded at recruitment （baseline） and at the end of the treatment （8 weeks）. The efficacy outcome included serum testosterone （total and free） levels, muscle strength and repetitions to failure, metabolic markers for anabolic activity （serum creatinine and blood urea nitrogen）, and % body fat. The standard safety measurements such as adverse events monitoring, vital signs, hematology, biochemistry, and urinalysis were performed. Results： Fenu-FG supplementation demonstrated significant anabolic and androgenic activity as compared with the placebo. Fenu-FG treated subjects showed significant improvements in body fat without a reduction in muscle strength or repetitions to failure. The Fenu-FG supplemen- tation was found to be safe and well-tolerated. Conclusion： Fenu-FG supplementation showed beneficial effects in male subjects during resistance training without any clinical side effects.
文摘Fenugreek and carob seed were germinated in the dark for 4 days and 15 days respectively. The two species are rich in vitamins A, B1, B3, B8. Germination promotes the increase in their concentrations. To ensure preservation and increased bioavailability of the vitamins, it is necessary to apply a DIC of 30s to 400 kPa making it possible to support the cellular expansion and the decontamination of germinated seeds. Vitamin A content in germinated carob seed increased by 82.54%, which is not the case for fenugreek. A decrease in vitamin A concentration of 4 μg/100 g of dry matter. DIC increase vitamin B1, B3 and B8 in germinated fenugreek seeds. A slight decrease of B vitamins in germinated carob seed after treatment with DIC has been noticed. This loss can be explained by the low bioavailability caused mainly by the botanical structures of the seeds.
文摘Background:Stress ulcers are common in mechanically ventilated patients.They are the most common cause of gastrointestinal bleeding and are primarily associated with increased the consumption of nonsteroidal anti-inflammatory drugs.Despite advanced treatment,it has a high in-hospital mortality rate.This study assessed the effect of fenugreek(Trigonella foenum-graceum L.)seeds on gastrointestinal bleeding in mechanically ventilated patients.Methods:In this double-blind,randomized controlled clinical trial,60 mechanically ventilated patients hospitalized in intensive care units were equally distributed into intervention and control groups.The intervention group received 3 mg fenugreek seeds powder twice daily with a gavage solution for five days,in addition to routine treatment.The control group received only the gavage solution and routine treatment.The gastrointestinal bleeding and abdominal pain at admission and during the five days were assessed.Data were analyzed using SPSS software V.16,and the significance level was set at 0.05.Results:Gastrointestinal bleeding at admission was similar in both groups.The number of mechanically ventilated patients with gastrointestinal bleeding decreased on the third,fourth,and fifth days,but the difference was insignificant.The Cochran test results showed that the rate of stress-induced gastrointestinal bleeding in the control group increased significantly(P=.04).The decrease in gastrointestinal bleeding in the intervention group was not statistically significant(P=.08).Conclusion:The use of this drug reduces the gastrointestinal side effects of ventilated patient.Despite this,reducing gastrointestinal bleeding and similar mechanical ventilation complications in patients entirely dependent on the treating team is an important achievement.
文摘The objective of the current work is to investigate the possible beneficial effects of forced feeding of fenugreek to rabbit does on their milk composition, milk yield, anemia screening parameters, immunity and the growth performance of bunnies. Twenty white New Zealand female rabbits were randomly assigned into 5 groups: normal control and 4 treated groups (vehicle (molasses), fenugreek powdered seeds + molasses, sprouted fenugreek + molasses, and fenugreek oil + molasses). All treated groups took the given doses orally for two days before parturition and 13 days after parturition for 2 successive pregnancies. Blood samples were collected from each doe on the 13<sup>th</sup> day of lactation for determination of hematological, biochemical and immunological parameters. Milk samples were taken for evaluation of milk composition. All rabbit does and their bunnies were weighed for calculation of the milk yield. The fenugreek seeds and oil administered groups (G<sup>3</sup> & G<sup>5</sup>) recorded the highest percent values of fat, protein, lactose, total solids and solid not fat contents of the rabbits’ milk in both 1st and 2nd lactation periods relative to other tested groups. At the beginning and mid of lactation periods, the milk yields recorded non-significant differences either between tested groups or between 1<sup>st</sup> and 2<sup>nd</sup> pregnancy in the same group. Whereas, at the end of lactation, germinated seeds (G<sup>4</sup>) and oil (G<sup>5</sup>) treated groups recorded significantly higher rates of milk yield relative to control and fenugreek seeds groups. Bunnies’ body weights suckling from dams of group III (G<sup>3</sup>) recorded the highest weights relative to other tested groups in both 1<sup>st</sup> and 2<sup>nd</sup> pregnancy. During the 1<sup>st</sup> lactation period, all experimental groups showed significant reduction of white blood cells, while, G<sup>3</sup> and G<sup>4</sup> recorded significant elevation during 2<sup>nd</sup> lactation period. Rabbits treated with fenugreek seeds powder (G<sup>3</sup>) showed a significant raise of phagocytic activity and phagocytic index. Fenugreek seeds group (G<sup>3</sup>) and germinated one (G<sup>4</sup>) showed significant red blood corpuscles and packed cell volume raise relative to other groups during both 1<sup>st</sup> and 2<sup>nd</sup> lactation periods.
文摘Adding different percentages 1%, 3% and 5% of fenugreek flour depending on maize flour base to gluten free bread recipe for making tanoor bread was used to improve the tanoor bread quality, in term of sensory properties like volume, crust, color, symmetry, bake uniformity, texture, grain, aroma and taste. Also some rheological properties like gelatinization temperature and maximum viscosity of dough with bread keeping quality were used. The aim of this work is to produce suitable bread with maintaining the bread quality by selecting the suitable percentage to produce gluten free bread for people who suffering from celiac diseases and its contribution to health benefits. Significant differences were observed by using 5% of fenugreek flour in term of gelatinization temperature, maximum viscosity, breed keeping quality and volume. While using 1% of fenugreek flour significantly improved symmetry value, bread texture, crumb color, aroma and taste.
文摘Seeds of Fenugreek （Trigonellafoenum-graecum L.）, an international spice commodity, are much consumed in Sudan as a food, for flavoring and as a folk-remedy for several ailments, together with local beliefs in nutraceutical benefits. The authors have noted an immense variability in colour and other morphological characters of Fenugreek seeds offered in local Sudanese markets and wondered whether this variability is extended to their chemical composition. Steroidal sapogenins are important chemical constituents of Fenugreek seeds in view of their potential as precursors for the commercial synthesis of steroid drugs and their continually revealed beneficial biological activities. In this study, thirty Sudanese Fenugreek seed accessions collected from different geographical regions in Sudan were analyzed for their 25α-, 25β- and total sapogenin content using a simple and specific infra-red spectroscopic method. The seed accessions exhibited much morphological variability particularly in outer seed coat color and size. Preparative TLC followed by gravimetric analysis showed that steroidal sapogenins, mostly diosgenin and yamogenin, represented more than 70% of the steroids of Fenugreek seeds. Infra-red spectroscopic analysis showed that total 25α-sapogenins （calculated as diosgenin） varied from 0.65% to 1.68%. Total 25β-sapogenin （calculated as yamogenin） varied from 0.38% to 2.03%. The content of total sapogenins （α ＋ β epimeric forms） varied between 1.24% and 3.0% of the oven-dry weight of seeds. β-sapogenins （mostly yamogenin） were dominant over α-sapogenins （diosgenin） in most Sudanese Fenugreek seed accessions.
基金supported by the Small Business Technology Transfer Program of the Department of Health and Human Services National Institutes of Health,United States.The grant number is 1R41AI152915-01.
文摘Alfalfa and fenugreek sprouts are healthy foods,but they are occasionally contaminated with bacterial pathogens and serve as vehicles for transmitting foodborne illnesses.This study examined the efficacy of ascaroside(ascr)#18 treatment for the control of enterohemorrhagic E.coli(EHEC)growth on sprouts.Commercial alfalfa and fenugreek seeds were decontaminated with 20,000 ppm of NaClO,and residual chlorine was neutralized with Dey-Engley broth.Decontaminated seeds were treated with 1 mM or 1μM ascr#18,a plant immunity modulator,before being dried and mixed with sandy soil inoculated with E.coli F4546 or BAA-2326 at 10^(4)-10^(5)CFU/g.The inoculated seeds were sprouted on 1%water agar at 25◦C for 7 days in the dark.Seed or sprout samples were collected on days 0,1,3,5,and 7 for enumeration of bacterial populations.Data was fit into the general linear model and analyzed using Fisher’s least significant different test of the statistical analysis software.Treatment with ascr#18 significantly(P≤0.05)reduced the cell population of EHEC on sprouts.The mean EHEC populations in the 1 mM or 1μM treatment groups were 3.31 or 1.56 log CFU/g lower compared to the control groups.Besides treatment,sprout seed type and sprouting time were also significant independent variables influencing the growth of EHEC,according to the results of type III error analysis.However,EHEC strain type was not a significant independent variable.The study suggests that ascr#18 could be potentially used to control EHEC contamination and improve the microbial safety of sprouts.
基金funded by the National Key Research and Devel-opment Program of China(Grant number:2022YFD2101400)Doctor Research Fund of the Henan University of Technology(grant number:2023BS086)+1 种基金the Henan University of Technology postdoctoral scientific research fund(grant number:21450081)the Open Project Program(Project Number)of State Key Laboratory of Rice Biology and Breeding(grant number:20240206).
文摘A novelβ-mannanase gene(ScMan26)from Stachybotrys chlorohalonata was expressed in Komagataella phaffii.ScMan26 displayed 69.1%of identity with theβ-mannanase from Thermothelomyces thermophilus ATCC 42464.The highestβ-mannanase yield of 45000 U mL^(-1) with protein concentration of 7.8 mg mL^(-1) was obtained in a 5-L fermenter.ScMan26 was most active at 55℃ and pH 6.5,respectively.ScMan26 efficiently hydrolyzed fenugreek gum(15%,w/v)to yield manno-oligosaccharides.The manno-oligosaccharides promoted the growth of Lactobacillus casei AS 1.62 and Bifidobacteria adolescentis ATCC 15703 by 1.8 and 1.7-fold,respectively,compared to the control.Thus,fenugreek manno-oligosaccharides prepared by ScMan26 may be useful as a prebiotic source.
基金supported by the Open Foundation Project of Sichuan Provincial Key Laboratory of Philosophy and Social Sciences in 2024(ZHYYZKYB2420).
文摘Saponins are a widely occurring class of secondary metabolites and are of variety of biological activities.Fenugreek,a saponin-rich traditional Chinese herb,has a long history in treatment of different kinds of disorders.In this study,total saponins of Fenugreek(TSOF)was successfully purified by a membrane-resin combined method(purity>90%)and its antioxidative activity was also tested.DPPH and ABTS tests demonstrated its potent radical scavenging activity with IC_(50) values of 0.163 mg/mL and 0.449 mg/mL,respectively.Furthermore,H_(2)O_(2)-induced H9c2 cells was used to identify the protective effect of TSOF.Results showed that TSOF could significantly reduce intracellular reactive oxygen species(ROS)production and malondialdehyde(MDA)level,and enhance intracellular superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSHPx)activities,thereby increasing cell viability and antagonizing cell damage.Morphological observation and LIVE/DEAD staining also demonstrated protective effect of TSOF on oxidative stress at cellular level.Our study presented a promising method for purification of fenugreek saponins.With determination of antioxidant activity of saponins,theoretical and data support were provided for development of fenugreek relative applications.
基金Supported by Department of Science and Technology(DST),University Grants Commission,New Delhi,India
文摘Objective: To evaluate the comparative effects of fenugreek(Trigonel a foenum graecum) seed extract(FSE) alone and in combination with an antidiabetic conventional medicine,glibenclamide(GLB),on the inhibition of in vitro lipid peroxidation(LPO) in liver,the major target organ of a drug.Methods: LPO was induced by ferrous sulphate(Fe So4),hydrogen peroxide(H2 O2) and carbon tetrachloride(CCl4) and the effects of test seed extract and/or GLB were evaluated.Results: While Fe So4,H2 O2 and CCl4 markedly enhanced the hepatic LPO,simultaneous administration of FSE reduced it in a concentration dependent manner.However,when both FSE and GLB were added to the incubation mixture,chemical y induced hepatic LPO was further inhibited.The test extract also exhibited high antioxidative activity in 1,1-diphenyl-2-picrylhydrazyl radical and in 2,2'-azino-bis,3-ethylbenzothiazoline-6-sulphonic acid radical scavenging assays.Conclusion: FSE therapy in moderate concentration along with a hypoglycemic drug may prove to be advantageous in ameliorating diabetes mel itus and other diseases that are LPO mediated.
