The main objective of this study was to investigate the relationship between a ceftriaxone-resistant Neisseria gonorrhoeae strain GX211022,isolated from China,and MLST ST13871 strains reported internationally.In this ...The main objective of this study was to investigate the relationship between a ceftriaxone-resistant Neisseria gonorrhoeae strain GX211022,isolated from China,and MLST ST13871 strains reported internationally.In this study,we determined the susceptibility of the N.gonorrhoeae isolate to antibiotics using the agar dilution method.Genotyping was performed through N.gonorrhoeae sequence typing for antimicrobial resistance(NG-STAR),N.gonorrhoeae multiantigen sequence typing(NG-MAST),and multilocus sequence typing(MLST).Additionally,phylogenetic analysis was conducted to explore the genetic relatedness to other MLST ST13871 strains.Isolate GX211022 was identified to possess penA allele 60.001 with a ceftriaxone MIC=1 mg/L and was classified as MLST ST13871 and NG-STAR ST233.Phylogenetic analysis indicated that GX211022 was clustered with isolates from Vietnam and confirmed that all MLST ST13871 strains are a subclone of the internationally disseminated high-level ceftriaxone-resistant FC428 clone.These findings highlight abundant regional spread of MLST ST13871 in Southeast Asia.展开更多
Neisseria gonorrhoeae is a multidrug-resistant bacterial pathogen for which ceftriaxone is the only remaining recommended first-line therapy.However,ceftriaxone susceptibility has been waning in a number of countries ...Neisseria gonorrhoeae is a multidrug-resistant bacterial pathogen for which ceftriaxone is the only remaining recommended first-line therapy.However,ceftriaxone susceptibility has been waning in a number of countries over the last decade and ceftriaxone treatment failures have been reported,commonly as a result of sporadic high-level ceftriaxone-resistant strains.In recent years,N.gonorrhoeae strains associated with the high-level ceftriaxone-resistant FC428 clone or strains that acquired its main ceftriaxone resistance determinant,penA allele 60.001,have shown global transmission,resulting in ceftriaxone treatment failure in a number of cases.The FC428 clone was first encountered in Japan in 2015 and subsequently in China,Europe,Australia,North America and Southeast Asia afterward.Strains associated with the FC428 clone commonly display a ceftriaxone minimum inhibitory concentration of 0.5-1 mg/L.However,where penA alleles encountered in sporadic high-level ceftriaxone-resistant isolates induce an in vitro growth defect,penA allele 60.001 does not seem to affect in vitro growth.The limited impact of penA allele 60.001 on biological fitness might be associated with its successful global transmission.Although the FC428 clone displays high-level ceftriaxone resistance,most gonorrhea cases associatedwith this clone were still successfully curedwith ceftriaxonewhen intramuscular or intravenous doses of 500mg to 2 g were used.A successful alternative therapy seems to be ertapenem given at 1-g doses,although further clinical studies are required to validate ertapenemefficacy.This review summarizes the global transmission of strains associated with the FC428 clone and antimicrobial treatment of associated cases.展开更多
基金supported by Fundamental Research Operations of the Central-level Public Welfare Research Institute of the Chinese Academy of Medical Sciences(2022-RW320-15)Jiangsu Provincial Medical Key Laboratory,Jiangsu Province Capability Improvement Project through Science,Technology and Education(ZDXYS202204).The funders had no role in study design,data collection or analysis,decision to publish,or preparation of the manuscript.
文摘The main objective of this study was to investigate the relationship between a ceftriaxone-resistant Neisseria gonorrhoeae strain GX211022,isolated from China,and MLST ST13871 strains reported internationally.In this study,we determined the susceptibility of the N.gonorrhoeae isolate to antibiotics using the agar dilution method.Genotyping was performed through N.gonorrhoeae sequence typing for antimicrobial resistance(NG-STAR),N.gonorrhoeae multiantigen sequence typing(NG-MAST),and multilocus sequence typing(MLST).Additionally,phylogenetic analysis was conducted to explore the genetic relatedness to other MLST ST13871 strains.Isolate GX211022 was identified to possess penA allele 60.001 with a ceftriaxone MIC=1 mg/L and was classified as MLST ST13871 and NG-STAR ST233.Phylogenetic analysis indicated that GX211022 was clustered with isolates from Vietnam and confirmed that all MLST ST13871 strains are a subclone of the internationally disseminated high-level ceftriaxone-resistant FC428 clone.These findings highlight abundant regional spread of MLST ST13871 in Southeast Asia.
基金supported by the National Natural Science Foundation of China(grant numbers 82272382,82150610507,82072320,81871695).
文摘Neisseria gonorrhoeae is a multidrug-resistant bacterial pathogen for which ceftriaxone is the only remaining recommended first-line therapy.However,ceftriaxone susceptibility has been waning in a number of countries over the last decade and ceftriaxone treatment failures have been reported,commonly as a result of sporadic high-level ceftriaxone-resistant strains.In recent years,N.gonorrhoeae strains associated with the high-level ceftriaxone-resistant FC428 clone or strains that acquired its main ceftriaxone resistance determinant,penA allele 60.001,have shown global transmission,resulting in ceftriaxone treatment failure in a number of cases.The FC428 clone was first encountered in Japan in 2015 and subsequently in China,Europe,Australia,North America and Southeast Asia afterward.Strains associated with the FC428 clone commonly display a ceftriaxone minimum inhibitory concentration of 0.5-1 mg/L.However,where penA alleles encountered in sporadic high-level ceftriaxone-resistant isolates induce an in vitro growth defect,penA allele 60.001 does not seem to affect in vitro growth.The limited impact of penA allele 60.001 on biological fitness might be associated with its successful global transmission.Although the FC428 clone displays high-level ceftriaxone resistance,most gonorrhea cases associatedwith this clone were still successfully curedwith ceftriaxonewhen intramuscular or intravenous doses of 500mg to 2 g were used.A successful alternative therapy seems to be ertapenem given at 1-g doses,although further clinical studies are required to validate ertapenemefficacy.This review summarizes the global transmission of strains associated with the FC428 clone and antimicrobial treatment of associated cases.
