目的:分析FBXO31(F-box only protein 31)对人宫颈癌细胞C-33A增殖的影响及其在人宫颈癌组织中的表达与临床因素间的相关性,探究其在人宫颈癌中的作用,以期寻找新的分子靶点。方法:MTT法分别检测不同浓度、不同时间时FBXO31作用下的C-33...目的:分析FBXO31(F-box only protein 31)对人宫颈癌细胞C-33A增殖的影响及其在人宫颈癌组织中的表达与临床因素间的相关性,探究其在人宫颈癌中的作用,以期寻找新的分子靶点。方法:MTT法分别检测不同浓度、不同时间时FBXO31作用下的C-33A细胞增殖抑制率。免疫组织化学法及蛋白质印迹(Western blotting)法检测70例人宫颈癌组织及癌旁组织中FBXO31的表达,并分析其与宫颈癌临床病理特征间的关系。结果:MTT法示,FBXO31作用后,C-33A细胞增殖受到抑制,且呈现浓度和时间依赖性。免疫组织化学法示,FBXO31主要表达于细胞核,人宫颈癌组织中FBXO31的阳性表达率(17/70,24.29%)明显低于癌旁组织(60/70,85.71%),差异有统计学意义(χ2=74.035,P<0.001)。Western blotting法示,人宫颈癌组织中FBXO31表达量明显低于癌旁组织,差异有统计学意义(P<0.05);进一步分析示FBXO31在宫颈癌组织中的表达与年龄、肿瘤大小及病理学类型无关,而与国际妇产科联盟(FIGO)分期、肿瘤分化程度及淋巴结转移和远处转移有关(P<0.05)。结论:FBXO31可能作为一种抑癌基因而抑制人宫颈癌C-33A细胞增殖,且呈时间及浓度依赖性。其在人宫颈癌组织呈低表达,且与宫颈癌临床特征关系密切,可能成为宫颈癌治疗新的分子靶点。展开更多
目的探讨FBXO31基因对肺腺癌细胞系A549增殖、克隆形成、迁移及侵袭能力的影响。方法通过脂质体转染法将FBXO31表达质粒及FBXO31 si RNA序列分别转染肺癌A549细胞,用Western blot、MTT、平板克隆、划痕和Transwell实验分别检测A549细胞...目的探讨FBXO31基因对肺腺癌细胞系A549增殖、克隆形成、迁移及侵袭能力的影响。方法通过脂质体转染法将FBXO31表达质粒及FBXO31 si RNA序列分别转染肺癌A549细胞,用Western blot、MTT、平板克隆、划痕和Transwell实验分别检测A549细胞中FBXO31蛋白表达、细胞增殖、克隆形成、迁移和侵袭能力。结果 Western blot显示FBXO31表达质粒转染上调A549细胞中FBXO31蛋白表达,而转染si RNA下调FBXO31蛋白表达。FBXO31基因过表达增强A549细胞增殖、克隆形成、侵袭及迁移能力,而FBXO31基因沉默抑制细胞增殖、克隆形成、侵袭及迁移能力(P<0.01)。结论 FBXO31基因可促进肺癌细胞增殖、克隆形成、迁移及侵袭能力。展开更多
Loss of Heterozygosity (LOH) is commonly considered to be one of a reason when some genes lose their function. Numbers of tumor suppressor genes are existing on the LOH lesion and chromosome 16q24 had been reported as...Loss of Heterozygosity (LOH) is commonly considered to be one of a reason when some genes lose their function. Numbers of tumor suppressor genes are existing on the LOH lesion and chromosome 16q24 had been reported as a LOH region in gastric cancer. Little is known about what kind of tumor suppressor genes locates around the position. F-box protein, (FBXO31) is a candidate tumor suppressor gene encoded in chromosome 16q24.3 and LOH of the gene was reported in breast cancer, hepatocellular carcinoma and ovarian cancer but the status of FBXO31 was not analyzed in gastric cancer so far. One hundred twenty-seven pairs of tumor and corresponding normal tissue specimens collected from gastric cancer patients who underwent gastrectomy. Total RNAs were extracted from those samples and the expression of FBXO31 was investigated using real time quantitative RT-PCR analysis. Patients were classified into FBXO31 high expression group and low expression group. Clinicopahological factors were compared between the two groups and importance of FBXO31 was investigated. The standardized expression of FBXO31 was not significantly different between tumor (0.43 ± 0.46) and the corresponding 0.49 ± 0.55 in normal tissue (p = 0.39). Two years survival rate was 77% in FBXO31 high expression group and 54% in low expression group however the chance of survival rate of high expression group was dropped in 5 years (Wilcoxon p = 0.01). Clinicopathological factors were compared between the two groups and peritoneal dissemination was observed significantly higher in FBXO31 low expression group than did in high expression group (p = 0.0398). In order to predict existence of peritoneal dissemination of gastric cancer before surgery, FBXO31 may become a favorite marker for the low risk of peritoneal dissemination.展开更多
This study highlights the role of C-terminal amide-bearing proteins(CTAPs)in protein homeostasis.Researchers discovered that CTAPs,formed under oxidative stress,are selectively recognized and degraded by the E3 ubiqui...This study highlights the role of C-terminal amide-bearing proteins(CTAPs)in protein homeostasis.Researchers discovered that CTAPs,formed under oxidative stress,are selectively recognized and degraded by the E3 ubiquitin ligase FBXO31 to maintain cellular health.Mutations in FBXO31,such as D334N,impair CTAP recognition,leading to abnormal protein accumulation and neurodevelopmental disorders.These findings not only elucidate the mechanism of protein quality control but also suggest potential therapeutic targets for diseases related to oxidative stress and protein misregulation.展开更多
文摘目的:分析FBXO31(F-box only protein 31)对人宫颈癌细胞C-33A增殖的影响及其在人宫颈癌组织中的表达与临床因素间的相关性,探究其在人宫颈癌中的作用,以期寻找新的分子靶点。方法:MTT法分别检测不同浓度、不同时间时FBXO31作用下的C-33A细胞增殖抑制率。免疫组织化学法及蛋白质印迹(Western blotting)法检测70例人宫颈癌组织及癌旁组织中FBXO31的表达,并分析其与宫颈癌临床病理特征间的关系。结果:MTT法示,FBXO31作用后,C-33A细胞增殖受到抑制,且呈现浓度和时间依赖性。免疫组织化学法示,FBXO31主要表达于细胞核,人宫颈癌组织中FBXO31的阳性表达率(17/70,24.29%)明显低于癌旁组织(60/70,85.71%),差异有统计学意义(χ2=74.035,P<0.001)。Western blotting法示,人宫颈癌组织中FBXO31表达量明显低于癌旁组织,差异有统计学意义(P<0.05);进一步分析示FBXO31在宫颈癌组织中的表达与年龄、肿瘤大小及病理学类型无关,而与国际妇产科联盟(FIGO)分期、肿瘤分化程度及淋巴结转移和远处转移有关(P<0.05)。结论:FBXO31可能作为一种抑癌基因而抑制人宫颈癌C-33A细胞增殖,且呈时间及浓度依赖性。其在人宫颈癌组织呈低表达,且与宫颈癌临床特征关系密切,可能成为宫颈癌治疗新的分子靶点。
文摘目的探讨FBXO31基因对肺腺癌细胞系A549增殖、克隆形成、迁移及侵袭能力的影响。方法通过脂质体转染法将FBXO31表达质粒及FBXO31 si RNA序列分别转染肺癌A549细胞,用Western blot、MTT、平板克隆、划痕和Transwell实验分别检测A549细胞中FBXO31蛋白表达、细胞增殖、克隆形成、迁移和侵袭能力。结果 Western blot显示FBXO31表达质粒转染上调A549细胞中FBXO31蛋白表达,而转染si RNA下调FBXO31蛋白表达。FBXO31基因过表达增强A549细胞增殖、克隆形成、侵袭及迁移能力,而FBXO31基因沉默抑制细胞增殖、克隆形成、侵袭及迁移能力(P<0.01)。结论 FBXO31基因可促进肺癌细胞增殖、克隆形成、迁移及侵袭能力。
文摘Loss of Heterozygosity (LOH) is commonly considered to be one of a reason when some genes lose their function. Numbers of tumor suppressor genes are existing on the LOH lesion and chromosome 16q24 had been reported as a LOH region in gastric cancer. Little is known about what kind of tumor suppressor genes locates around the position. F-box protein, (FBXO31) is a candidate tumor suppressor gene encoded in chromosome 16q24.3 and LOH of the gene was reported in breast cancer, hepatocellular carcinoma and ovarian cancer but the status of FBXO31 was not analyzed in gastric cancer so far. One hundred twenty-seven pairs of tumor and corresponding normal tissue specimens collected from gastric cancer patients who underwent gastrectomy. Total RNAs were extracted from those samples and the expression of FBXO31 was investigated using real time quantitative RT-PCR analysis. Patients were classified into FBXO31 high expression group and low expression group. Clinicopahological factors were compared between the two groups and importance of FBXO31 was investigated. The standardized expression of FBXO31 was not significantly different between tumor (0.43 ± 0.46) and the corresponding 0.49 ± 0.55 in normal tissue (p = 0.39). Two years survival rate was 77% in FBXO31 high expression group and 54% in low expression group however the chance of survival rate of high expression group was dropped in 5 years (Wilcoxon p = 0.01). Clinicopathological factors were compared between the two groups and peritoneal dissemination was observed significantly higher in FBXO31 low expression group than did in high expression group (p = 0.0398). In order to predict existence of peritoneal dissemination of gastric cancer before surgery, FBXO31 may become a favorite marker for the low risk of peritoneal dissemination.
基金supported by the Key Research and Development Program of Zhejiang Province(2025C02058)the National Natural Science Foundation of China(32000799).
文摘This study highlights the role of C-terminal amide-bearing proteins(CTAPs)in protein homeostasis.Researchers discovered that CTAPs,formed under oxidative stress,are selectively recognized and degraded by the E3 ubiquitin ligase FBXO31 to maintain cellular health.Mutations in FBXO31,such as D334N,impair CTAP recognition,leading to abnormal protein accumulation and neurodevelopmental disorders.These findings not only elucidate the mechanism of protein quality control but also suggest potential therapeutic targets for diseases related to oxidative stress and protein misregulation.
