AIM:To investigate whether vaccinia-related kinase 1(VRK1)mediates transforming growth factor-beta2(TGF-β2)-caused epithelial-mesenchymal transition(EMT)and inflammatory responses in retinal pigment epithelial(RPE)ce...AIM:To investigate whether vaccinia-related kinase 1(VRK1)mediates transforming growth factor-beta2(TGF-β2)-caused epithelial-mesenchymal transition(EMT)and inflammatory responses in retinal pigment epithelial(RPE)cells through regulating snail family transcriptional repressor 1(SNAI1),and to validate its role in a proliferative vitreoretinopathy(PVR)mouse model.METHODS:Human RPE cell line ARPE-19 cells were treated with TGF-β2 to construct an EMT model.Western blot detected VRK1 level.The effects of VRK1 on SNAI1 expression and biological behavior of ARPE-19 cells were detected by immunofluorescence,ELISA,Transwell,and scratch assay,and the interaction between VRK1 and SNAI1 was confirmed through immunoprecipitation.A PVR mouse model was constructed,and the effects of VRK1 or/and SNAI1 on retinal damage were assessed by pathologic staining.Inflammatory factors and EMT-related proteins were assessed with ELISA and Western blot.RESULTS:VRK1 was upregulated in ARPE-19 cells after TGF-β2 treatment.Overexpression of VRK1 increased cell viability,promoted cell migration and EMT,and the levels of inflammatory factors.Silencing of VRK1 reversed the above indexes.There was a direct interaction between VRK1 and SNAI1,and overexpresssion SNAI1 weakened the impacts of silencing of VRK1.In PVR mice,silencing of VRK1 ameliorated retinal structural damage,decreased proinflammatory factor levels,and suppressed SNAI1 and mesenchymal marker expression.SNAI1 overexpression antagonized the protective effects of silencing VRK1 and exacerbated EMT and inflammatory responses.CONCLUSION:VRK1 plays a key role in retinal structural and inflammatory damage in PVR mice by regulating SNAI1 and mediating TGF-β2-caused EMT and inflammatory responses in RPE cells.展开更多
Dear Editor,We reported a Chinese family carrying a novel Crumbs homologue 1(CRB1)variant(c.1737_1755del).Consanguineous marriage resulted in a homozygous mutation,leading to the onset of Leber congenital amaurosis(LC...Dear Editor,We reported a Chinese family carrying a novel Crumbs homologue 1(CRB1)variant(c.1737_1755del).Consanguineous marriage resulted in a homozygous mutation,leading to the onset of Leber congenital amaurosis(LCA)in their offspring.展开更多
Background:Receptor-interacting protein kinases(RIPKs)regulate cell death,inflammation,and immune responses,yet their roles in cancer are not fully understood.This study investigates the expression,genomic alterations...Background:Receptor-interacting protein kinases(RIPKs)regulate cell death,inflammation,and immune responses,yet their roles in cancer are not fully understood.This study investigates the expression,genomic alterations,and functional implications of RIPK family members across various cancers.Methods:We collected multi-omics data from The Cancer Genome Atlas and other public databases,including gene expression,copy number variation(CNV),mutation,methylation,tumor mutation burden(TMB),and microsatellite instability(MSI).Differential expression and survival analyses were performed using DESeq2 and Cox proportional hazards models.CNV and mutation data were analyzed with GISTIC2 and Mutect2,and methylation data with the ChAMP package.Correlations with TMB and MSI were assessed using Pearson coefficients,and gene set enrichment analysis was conducted with the MSigDB Hallmark gene sets.Results:RIPK family members show significant differential expression in various cancers,with RIPK1 and RIPK4 frequently altered.Survival analysis reveals heterogeneous impacts on overall survival.CNV and mutation analyses identify high alteration frequencies for RIPK2 and RIPK7,affecting gene expression.RIPK1 and RIPK7 are hypermethylated in several cancers,inversely correlating with RIPK3 expression.RIPK1,RIPK2,RIPK5,RIPK6,and RIPK7 correlate positively with TMB,while RIPK3 shows negative correlations in some cancers.MSI analysis indicates associations with DNA mismatch repair.G ene set enrichment analysis highlights immune-related pathway enrichment for RIPK1,RIPK2,RIPK3,and RIPK6,and cell proliferation and DNA repair pathways for RIPK4 and RIPK5.RIPK family members showed heterogeneous alterations across cancers:for example,RIPK7 was mutated in up to~15%of u terine c orpus e ndometrial c arcinoma and l ung s quamous c ell c arcinoma cases,and RIPK1 and RIPK7 exhibited frequent promoter hypermethylation in multiple tumor types.Several genes displayed context-dependent associations with overall survival and with TMB/MSI.Conclusion:This pan-cancer analysis of the RIPK family reveals their diverse roles and potential as biomarkers and therapeutic targets.The findings emphasize the importance of RIPK genes in tumorigenesis and suggest context-dependent functions across cancer types.Further studies are needed to explore their mechanisms in cancer development and clinical applications.展开更多
新学期伊始,翻开鲁科版五四制小学英语的第七单元,《Family》的标题赫然在目,和所有英语老师一样,我对这个单元的内容、流程再熟悉不过了。按照以往的经验,我会带着孩子们认读图片、跟读录音、分组进行角色扮演,最后再进行一次基础小测...新学期伊始,翻开鲁科版五四制小学英语的第七单元,《Family》的标题赫然在目,和所有英语老师一样,我对这个单元的内容、流程再熟悉不过了。按照以往的经验,我会带着孩子们认读图片、跟读录音、分组进行角色扮演,最后再进行一次基础小测验。孩子们大多能流利地指着图片说出“This is my father.”但我总感觉缺了点什么,这些词汇对于他们而言,似乎只是一张需要死记硬背的单词表,与那个充满烟火气、承载着他们所有喜怒哀乐的“家”,隔着一层看不见的屏障。直到我看到坐在窗边的小雅,在课本“全家福”插图的旁边,用铅笔认真画了一个歪歪扭扭的小人,旁边工工整整地写着“me”。展开更多
Osmanthus fragrans is most famous for its strong aroma,and different varieties have different degrees of fragrance and color.Fragrance and color are important factors affecting the ornamental quality of O.fragrans.Ter...Osmanthus fragrans is most famous for its strong aroma,and different varieties have different degrees of fragrance and color.Fragrance and color are important factors affecting the ornamental quality of O.fragrans.Terpenoids are important secondary metabolites in plants,with β-carotene(C40)being the major pigment substance and linalool(C10)being the key aromatic component in O.fragrans.The geranylgeranyl pyrophosphate synthase genes(GGPPSs)play important roles in secondary metabolism in plants.However,the functions of the GGPPS family in floral color and fragrance formation has rarely been reported in O.fragrans.In this study,24 Of GGPPS genes were identified and classified into two subfamilies.The Of GGPPSs showed tissue-specific expression and Of GGPPS13 had highest expression in flowers.The Of GGPPS13 protein was localized to chloroplasts.The transcriptome data of Of GGPPS13 was verified by q RT-PCR and the expression level in‘Wanyingui'with strong aroma was higher than that in‘Zhuangyuanhong'with deep color at different flower development stages.Transient overexpression of Of GGPPS13 in O.fragrans petals showed that Of GGPPS13 increased the β-carotene content,the main color substance of O.fragrans,but decreased the linalool content,the main volatile organic compound(VOC)in the floral aroma of O.fragrans.Of GGPPS13 was indicated as the critical gene related to terpenoid synthesis in the floral aroma and color formation in O.fragrans.Our findings provide gene resources on the GGPPS gene family for further revealing the molecular regulation mechanism of the floral color and aroma formation in O.fragrans.展开更多
Background and Purpose: In recent years, individual spirituality has been attracting attention, but little research has been conducted as it relates to family spirituality that applies this concept to the family and r...Background and Purpose: In recent years, individual spirituality has been attracting attention, but little research has been conducted as it relates to family spirituality that applies this concept to the family and relates to the meaning of the family’s existence in terms of the entire family. The purpose of this study was to clarify the attributes of family spirituality and the influencing factors of its decline. Methods: Regarding family spirituality, 1) a literature search was conducted using PubMed and reviews of 20 English-language articles;and 2) semi-structured interviews were conducted with 12 Japanese families having elderly members in the household. Data triangulation was performed for both, and a directed content analysis was conducted using Hohashi’s Concentric Sphere Family Environment Theory as the framework. Results: Attributes of family spirituality included 21 categories, such as “I think that my family exists for my children and grandchildren.” Factors influencing the decline in family spirituality included 20 categories in total, including 6 categories of risk/causal/promoting factors such as “lack of caring for family members”;11 categories of preventive/inhibitory/suppression factors such as “healthcare professionals not being close to the family”;and three categories of context-sensitive factors such as “death of a family member.” Conclusions/Implications for Practice: Family intervention requires nurses to understand the attributes of family spirituality and to control the influencing factors of a decline in family spirituality. Through such efforts, families will be able to discover the meaning of the existence of the family and maintain and improve their well-being.展开更多
Recent literature has focused on the venture capital investments of large family firms made through their family offices.However,evidence regarding informal venture capital provided to nascent entrepreneurs by small f...Recent literature has focused on the venture capital investments of large family firms made through their family offices.However,evidence regarding informal venture capital provided to nascent entrepreneurs by small family businesses is scant.I contribute to the literature by modeling the angel investments of about 30,000 family and nonfamily business entrepreneurs across 49 countries,who contributed about$64 million to businesses started by other entrepreneurs over a 3-year period.Based on this sample,I conclude that in countries with higher rates of small family businesses,angel investors are likely fewer,and the amounts invested in start-ups are also lower.Furthermore,I conclude that the owners/managers of small family businesses invest comparatively less capital in start-ups,irrespective of family relationships.I infer that this is attributable to the financing preferences of family businesses,which favor the use of internal resources for their continuity and the growth of their family capital.展开更多
Published:25 September 2025 In the article“MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2”(BIOCELL,2021,Vol.45,No.3,pp.627-638.doi:10.32604/biocell.2021.01349...Published:25 September 2025 In the article“MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2”(BIOCELL,2021,Vol.45,No.3,pp.627-638.doi:10.32604/biocell.2021.013496),there is an error in the Fig.3.In order to ensure the scientific and rigorous nature of our academic publications,we made a correction about Fig.3 correspondingly.展开更多
Objective:To understand the current status and influencing factors of family caregiving for infants and toddlers aged 6–36 months in Tianhe District,Guangzhou City,with the aim of better comprehending the physiologic...Objective:To understand the current status and influencing factors of family caregiving for infants and toddlers aged 6–36 months in Tianhe District,Guangzhou City,with the aim of better comprehending the physiological and psychological needs of infants and toddlers,and assisting families in developing effective intervention measures and support strategies.Methods:In November 2024,a random sample of 311 infants and toddlers aged 6–36 months from the permanent resident population in Tianhe District,Guangzhou City,was selected.Caregivers were surveyed using a questionnaire regarding family caregiving practices for infants and toddlers.The Kruskal-Wallis’s test,Mann-Whitney test,and multiple linear regression analysis were employed to examine the influencing factors of family caregiving for infants and toddlers.Results:The findings indicate that the quality of family caregiving is significantly influenced by the educational level of the parents(p<0.001)and the annual household income(p<0.001).Work-family conflict showed a significant negative correlation with the quality of family caregiving(β=-0.239,p<0.001),while family atmosphere demonstrated a significant positive correlation with the quality of family caregiving(β=0.225,p<0.001).Conclusion:In Tianhe District,Guangzhou City,the quality of infant and toddler caregiving is determined by a combination of key factors,including annual household income,parental educational background,work-family conflict,and family atmosphere.These elements collectively form the core determinants of caregiving practices for infants and toddlers.展开更多
BACKGROUND Colorectal cancer(CRC)is the third most common cancer globally,causing over 900000 deaths annually.Risk factors include aging,diet,obesity,sedentary lifestyle,tobacco use,genetic predisposition,and inflamma...BACKGROUND Colorectal cancer(CRC)is the third most common cancer globally,causing over 900000 deaths annually.Risk factors include aging,diet,obesity,sedentary lifestyle,tobacco use,genetic predisposition,and inflammatory bowel disease.Despite current treatments,survival rates for advanced CRC remain low,highlighting the need for better therapeutic strategies.AIM To evaluate both the clinical significance and the pathological implications of the Kinesin family member 14(KIF14)expression within CRC specimens.Additionally,this study aims to investigate the interaction between nitidine chloride(NC)and KIF14,considering their potential as therapeutic targets.METHODS The expression of the KIF14 protein in CRC was analyzed using immunohistochemical staining.The integration of multicenter high-throughput data facilitated the calculation of the standardized mean difference(SMD)for KIF14 mRNA levels.The assessment of clinical and pathological impact was enhanced by analyzing combined receiver operating characteristic curves,along with measures of sensitivity,specificity,and likelihood ratios.Additionally,clustered regularly interspaced short palindromic repeats knockout screening for cell growth and single-cell sequencing were employed to validate the significance of KIF14 expression in CRC.Survival analysis established the prognostic value of KIF14 in CRC.The molecular mechanism of NC against CRC was elucidated through whole-genome sequencing and enrichment analysis,and molecular docking was utilized to explore the targeting affinity between NC and KIF14.RESULTS KIF14 was highly expressed in 208 CRC patients.Data from 17 platforms involving 2436 CRC samples and 1320 noncancerous colorectal tissue controls indicated that KIF14 expression was significantly higher in CRC samples,with an SMD of 1.92(95%CI:1.49-2.35).The area under the curve was 0.94(95%CI:0.92-0.96),with a sensitivity of 0.85(95%CI:0.78-0.90)and a specificity of 0.90(95%CI:0.85-0.93).The positive and negative likelihood ratios were 8.38(95%CI:5.39-13.02)and 0.17(95%CI:0.11-0.26),respectively.At the single-cell level,significant overexpression of KIF14 was observed in CRC cells(P<0.001),with 35 CRC cell lines dependent on KIF14 for growth.The K-M plots demonstrated that KIF14 possesses prognostic value in CRC patients within the GSE71187 and GSE103679 datasets(P<0.05).Binding energy calculations indicated that KIF14 is a potential target for NC(binding energy:10.3 kcal/mol).CONCLUSION KIF14 promotes the growth of CRC cells and acts as an oncogenic factor,potentially serving as a therapeutic target for NC in the treatment of CRC.展开更多
In this editorial,we comment on the article by Qin et al,recently published in the World Journal of Gastrointestinal Oncology.Malignant tumors of the digestive tract represent a significant health threat.Kinesin famil...In this editorial,we comment on the article by Qin et al,recently published in the World Journal of Gastrointestinal Oncology.Malignant tumors of the digestive tract represent a significant health threat.Kinesin family member 14(KIF14),a critical kinesin,is pivotal in the proliferation,migration,and invasion of tumor cells.It has emerged as a focal point in recent studies of malignant tumors in the digestive tract.This article reviews the current research on KIF14 within these tumors and details its significant role in tumor cell behaviors,including proliferation,apo-ptosis,migration,invasion,and angiogenesis,alongside the regulatory mechanisms of the associated intracellular signaling pathways.Additionally,it explores the clinical value of KIF14 as a potential biomarker for early diagnosis,disease monitoring,and prognostic evaluation in malignant tumors of the digestive tract.The article concludes by introducing the potential regulatory role of traditional Chinese medicine,aiming to combine the strengths of both modern and traditional medical approaches to enhance treatment outcomes and prognosis for patients with these tumors.展开更多
Wax gourd(Benincasa hispida)is an important cucurbit crop with economic and medicinal value.The myeloblastosis(MYB)gene family is one of the largest gene families in plants and regulates various biological processes,w...Wax gourd(Benincasa hispida)is an important cucurbit crop with economic and medicinal value.The myeloblastosis(MYB)gene family is one of the largest gene families in plants and regulates various biological processes,whereas the MYB gene family has not been systematically studied in wax gourd.In this study,we performed genome-wide identification of the MYB gene family in wax gourd and analyzed their phylogenetic relationship,MYB DNA-binding domain(MYB DBD),gene structure,protein motif,synteny,duplication mode and expression pattern.As a result,a total of 215 BhMYB genes(BhMYBs)were identified,belonging to four subfamilies:1R-,2R-,3R-and 4R-MYB subfamilies.Genes of 1R-MYB subfamily and 2R-MYB subfamily were subdivided into different subgroups respectively.The analysis of MYB DBD,gene structure and protein motif showed that the most genes in the same subgroup had similar characteristics and the 2R-MYB genes were more conserved than the 1R-MYB genes.Interestingly,the long terminal retrotransposons(LTR-RTs)were found in the long introns of several BhMYBs.The results of synteny analysis showed that there were more syntenic gene pairs between wax gourd and other cucurbit crops,while the least number of syntenic gene pairs existed between wax gourd and rice.Gene duplication was the main reason for the expansion of the MYB gene family in wax gourd,with the transposed duplication(TRD)mode contributing more.All duplication BhMYB genes were under purifying selection pressure.Further expression analysis showed that many BhMYBs exhibited obvious tissue-specific expression and several BhMYBs were significantly induced by one or more abiotic stresses.BhMYB79 was particularly expressed in roots and significantly induced by salt,drought,cold and heat stresses,overexpression of which led to reduced tolerance to salt stress in Arabidopsis.In conclusion,our results provide a systematic analysis of wax gourd MYB gene family and facilitate the biological role study of BhMYB79 during wax gourd salt stress response process.展开更多
The analysis of the historical and theoretical origins of the dual identity of Chinese women based on the Marxist view of family-society enables a more scientific and reasonable explanation to the unity of history and...The analysis of the historical and theoretical origins of the dual identity of Chinese women based on the Marxist view of family-society enables a more scientific and reasonable explanation to the unity of history and logic of Chinese women’s dual identity.Under the guidance of the Marxist concept of family-society,Chinese women will achieve development in both family space and social space;meanwhile,Chinese women in the dual identity development carry the home-state emotion different from that of western women’s;cultivating women’s awareness of family identity is of great significance to the construction of family style or national style in the new era of socialism with Chinese characteristics.展开更多
I have a wonderful family.We all like music very much.My mother is a music teacher.She is good at playing the piano and the violin.She also likes singing and she can sing many songs.My father is a doctor.He likes musi...I have a wonderful family.We all like music very much.My mother is a music teacher.She is good at playing the piano and the violin.She also likes singing and she can sing many songs.My father is a doctor.He likes music,too.After he comes back from work,he often listens to music.In fact,playing the flute and the erhu are his favorites.I enjoy playing the guitar though it's difficult to play.I practice playing it every day,so I can play it very well.I once won the second prize at a guitar concert in the city.But my sister thinks playing theguitar is boring.展开更多
Hi! I'm Linda.I'm 12 years old.I'm a student.I'm in No.1 middle school(1).Here is a nice photo of my family.These are my parents,Tom and Mary.My mother is a(2)English teacher.My father is a doctor.Thos...Hi! I'm Linda.I'm 12 years old.I'm a student.I'm in No.1 middle school(1).Here is a nice photo of my family.These are my parents,Tom and Mary.My mother is a(2)English teacher.My father is a doctor.Those are my brother and sister.My sister(3)name is Helen.She is 6 years old.My brother is Eric.展开更多
一、请根据录音,填写单词。二、听对话,选择正确的答案。1.Who is coming for dinner?A.Friends.B.Aunt and uncle.C.Grandma and grandpa.2.What does Dad offer to do at the beginning?A.Go shopping.B.Set the table.C.Cook the fish.
Objective:Cardiopulmonary resuscitation(CPR)is one of the most important life-saving procedures in the hospital.Contrary to medical guidelines,family presence during CPR is still not accepted in some countries.Family ...Objective:Cardiopulmonary resuscitation(CPR)is one of the most important life-saving procedures in the hospital.Contrary to medical guidelines,family presence during CPR is still not accepted in some countries.Family presence during CPR depends on the nurses’attitude,which is influenced by various factors.Emotional intelligence(EI)helps nurses make wise decisions and display responsible behavior,which is necessary for proper and good performance in nursing.The study’s purpose was to determine the attitude of acute care nurses toward family presence during CPR and its relationship with EI.Methods:The descriptive-analytical study was conducted among the nurses of acute care units(intensive care unit[ICU],critical care unit,and emergency department)in teaching hospitals of Qazvin University of Medical Sciences,Iran,in 2022-2023.A total of 186 nurses were included in the study using convenience sampling.The data collection tools were a checklist of demographic characteristics,nurses’attitude toward the presence of family scale,and Siberia Schering’s EI questionnaire.The collected data were analyzed using descriptive and analytical statistics and SPSS software.Results:The mean age of the participants was(32.05±6.93)years.Of 186 nurses participating in the study,127(68.3%)were women and the rest were men.The mean score of attitude was 47.41±9.41(the minimum score of the nurses was 19 and the maximum score was 95).The mean score of EI was 99.27±8.86(the minimum score was 69 and the maximum score was 128).The results of Pearson’s correlation coefficient showed no significant relationship between the total score of attitude and EI of the participants(P=0.588).Data analysis showed that only the self-arousal dimension of EI has a significant relationship with the range of family self-control behaviors in attitude(P=0.037).Conclusions:The results showed that the nurses of acute care units have a positive attitude toward the presence of the family during CPR and have high EI.Although no significant relationship was observed between the 2 variables,paying attention to the influencing factors on the attitude of the nurse toward family presence during CPR in different societies needs more research and investigation.展开更多
Background:Physical inactivity among adolescents has become a global public health challenge,with over 80%failing to meet the recommendations of the WHO for activity levels.Existing research predominantly examines how...Background:Physical inactivity among adolescents has become a global public health challenge,with over 80%failing to meet the recommendations of the WHO for activity levels.Existing research predominantly examines how physical activity(PA)enhances resilience,while the predictive role of resilience in PA,particularly its interaction with family factors,has received limited attention.This study aimed to examine the associations between resilience and PA among adolescents,focusing on family functioning and gender differences.Methods:In this cross-sectional study,a total of 909 Chinese adolescents(463 males and 446 females,aged 13.30.5 years)completed the±following validated self-report instruments:the Connor-Davidson Resilience Scale with 10 items,the Physical Activity Questionnaire for Older Children,and the Family Adaptability and Cohesion Evaluation Scale II-Chinese version that was used to categorize family functioning into three types(i.e.,lower,balanced,and higher).The generalized linear mixed-effect model(GLMM)was used to determine the contribution of resilience and its interaction with family functioning type on PA after adjusting for age and gender.Results:Males presented significantly higher resilience and PA levels(both p 0.001)as well as better family functioning(p 0.01)than females.Compared with the lower<<functioning group,participants from higher-functioning families showed superior resilience and PA(both p 0.001).<The GLMM analysis revealed a positive relationship between resilience and PA(p 0.001),where the lower functioning<group was significantly weaker than the higher functioning group.Conclusion:Resilience and PA in adolescents vary across gender and family functioning type,with males and adolescents from better-functioning families outperforming their peers.Resilience is a positive predictor of PA in adolescents,with family functioning type being a crucial moderator of such a relationship.展开更多
文摘AIM:To investigate whether vaccinia-related kinase 1(VRK1)mediates transforming growth factor-beta2(TGF-β2)-caused epithelial-mesenchymal transition(EMT)and inflammatory responses in retinal pigment epithelial(RPE)cells through regulating snail family transcriptional repressor 1(SNAI1),and to validate its role in a proliferative vitreoretinopathy(PVR)mouse model.METHODS:Human RPE cell line ARPE-19 cells were treated with TGF-β2 to construct an EMT model.Western blot detected VRK1 level.The effects of VRK1 on SNAI1 expression and biological behavior of ARPE-19 cells were detected by immunofluorescence,ELISA,Transwell,and scratch assay,and the interaction between VRK1 and SNAI1 was confirmed through immunoprecipitation.A PVR mouse model was constructed,and the effects of VRK1 or/and SNAI1 on retinal damage were assessed by pathologic staining.Inflammatory factors and EMT-related proteins were assessed with ELISA and Western blot.RESULTS:VRK1 was upregulated in ARPE-19 cells after TGF-β2 treatment.Overexpression of VRK1 increased cell viability,promoted cell migration and EMT,and the levels of inflammatory factors.Silencing of VRK1 reversed the above indexes.There was a direct interaction between VRK1 and SNAI1,and overexpresssion SNAI1 weakened the impacts of silencing of VRK1.In PVR mice,silencing of VRK1 ameliorated retinal structural damage,decreased proinflammatory factor levels,and suppressed SNAI1 and mesenchymal marker expression.SNAI1 overexpression antagonized the protective effects of silencing VRK1 and exacerbated EMT and inflammatory responses.CONCLUSION:VRK1 plays a key role in retinal structural and inflammatory damage in PVR mice by regulating SNAI1 and mediating TGF-β2-caused EMT and inflammatory responses in RPE cells.
基金Supported by the National Natural Science Foundation of China(No.81970804)Natural Science Foundation of Hunan Province(No.2021JJ30949).
文摘Dear Editor,We reported a Chinese family carrying a novel Crumbs homologue 1(CRB1)variant(c.1737_1755del).Consanguineous marriage resulted in a homozygous mutation,leading to the onset of Leber congenital amaurosis(LCA)in their offspring.
基金supported by grants from the Tianjin Health Technology Project(Grant no.2022QN106).
文摘Background:Receptor-interacting protein kinases(RIPKs)regulate cell death,inflammation,and immune responses,yet their roles in cancer are not fully understood.This study investigates the expression,genomic alterations,and functional implications of RIPK family members across various cancers.Methods:We collected multi-omics data from The Cancer Genome Atlas and other public databases,including gene expression,copy number variation(CNV),mutation,methylation,tumor mutation burden(TMB),and microsatellite instability(MSI).Differential expression and survival analyses were performed using DESeq2 and Cox proportional hazards models.CNV and mutation data were analyzed with GISTIC2 and Mutect2,and methylation data with the ChAMP package.Correlations with TMB and MSI were assessed using Pearson coefficients,and gene set enrichment analysis was conducted with the MSigDB Hallmark gene sets.Results:RIPK family members show significant differential expression in various cancers,with RIPK1 and RIPK4 frequently altered.Survival analysis reveals heterogeneous impacts on overall survival.CNV and mutation analyses identify high alteration frequencies for RIPK2 and RIPK7,affecting gene expression.RIPK1 and RIPK7 are hypermethylated in several cancers,inversely correlating with RIPK3 expression.RIPK1,RIPK2,RIPK5,RIPK6,and RIPK7 correlate positively with TMB,while RIPK3 shows negative correlations in some cancers.MSI analysis indicates associations with DNA mismatch repair.G ene set enrichment analysis highlights immune-related pathway enrichment for RIPK1,RIPK2,RIPK3,and RIPK6,and cell proliferation and DNA repair pathways for RIPK4 and RIPK5.RIPK family members showed heterogeneous alterations across cancers:for example,RIPK7 was mutated in up to~15%of u terine c orpus e ndometrial c arcinoma and l ung s quamous c ell c arcinoma cases,and RIPK1 and RIPK7 exhibited frequent promoter hypermethylation in multiple tumor types.Several genes displayed context-dependent associations with overall survival and with TMB/MSI.Conclusion:This pan-cancer analysis of the RIPK family reveals their diverse roles and potential as biomarkers and therapeutic targets.The findings emphasize the importance of RIPK genes in tumorigenesis and suggest context-dependent functions across cancer types.Further studies are needed to explore their mechanisms in cancer development and clinical applications.
文摘新学期伊始,翻开鲁科版五四制小学英语的第七单元,《Family》的标题赫然在目,和所有英语老师一样,我对这个单元的内容、流程再熟悉不过了。按照以往的经验,我会带着孩子们认读图片、跟读录音、分组进行角色扮演,最后再进行一次基础小测验。孩子们大多能流利地指着图片说出“This is my father.”但我总感觉缺了点什么,这些词汇对于他们而言,似乎只是一张需要死记硬背的单词表,与那个充满烟火气、承载着他们所有喜怒哀乐的“家”,隔着一层看不见的屏障。直到我看到坐在窗边的小雅,在课本“全家福”插图的旁边,用铅笔认真画了一个歪歪扭扭的小人,旁边工工整整地写着“me”。
基金funded by the National Natural Science Foundation of China(32071828 and 32471943)the Central Finance Forestry Science and Technology Promotion Demonstration Project,China(Su2024TG04)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),Chinapartly supported by the open funds of the National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops,China。
文摘Osmanthus fragrans is most famous for its strong aroma,and different varieties have different degrees of fragrance and color.Fragrance and color are important factors affecting the ornamental quality of O.fragrans.Terpenoids are important secondary metabolites in plants,with β-carotene(C40)being the major pigment substance and linalool(C10)being the key aromatic component in O.fragrans.The geranylgeranyl pyrophosphate synthase genes(GGPPSs)play important roles in secondary metabolism in plants.However,the functions of the GGPPS family in floral color and fragrance formation has rarely been reported in O.fragrans.In this study,24 Of GGPPS genes were identified and classified into two subfamilies.The Of GGPPSs showed tissue-specific expression and Of GGPPS13 had highest expression in flowers.The Of GGPPS13 protein was localized to chloroplasts.The transcriptome data of Of GGPPS13 was verified by q RT-PCR and the expression level in‘Wanyingui'with strong aroma was higher than that in‘Zhuangyuanhong'with deep color at different flower development stages.Transient overexpression of Of GGPPS13 in O.fragrans petals showed that Of GGPPS13 increased the β-carotene content,the main color substance of O.fragrans,but decreased the linalool content,the main volatile organic compound(VOC)in the floral aroma of O.fragrans.Of GGPPS13 was indicated as the critical gene related to terpenoid synthesis in the floral aroma and color formation in O.fragrans.Our findings provide gene resources on the GGPPS gene family for further revealing the molecular regulation mechanism of the floral color and aroma formation in O.fragrans.
文摘Background and Purpose: In recent years, individual spirituality has been attracting attention, but little research has been conducted as it relates to family spirituality that applies this concept to the family and relates to the meaning of the family’s existence in terms of the entire family. The purpose of this study was to clarify the attributes of family spirituality and the influencing factors of its decline. Methods: Regarding family spirituality, 1) a literature search was conducted using PubMed and reviews of 20 English-language articles;and 2) semi-structured interviews were conducted with 12 Japanese families having elderly members in the household. Data triangulation was performed for both, and a directed content analysis was conducted using Hohashi’s Concentric Sphere Family Environment Theory as the framework. Results: Attributes of family spirituality included 21 categories, such as “I think that my family exists for my children and grandchildren.” Factors influencing the decline in family spirituality included 20 categories in total, including 6 categories of risk/causal/promoting factors such as “lack of caring for family members”;11 categories of preventive/inhibitory/suppression factors such as “healthcare professionals not being close to the family”;and three categories of context-sensitive factors such as “death of a family member.” Conclusions/Implications for Practice: Family intervention requires nurses to understand the attributes of family spirituality and to control the influencing factors of a decline in family spirituality. Through such efforts, families will be able to discover the meaning of the existence of the family and maintain and improve their well-being.
基金support from Programa de Apoyo a la Investigacion(PAI)2023,Universidad Adolfo IbanezFONDECYT Grant No.1240098 is greatly acknowledged.
文摘Recent literature has focused on the venture capital investments of large family firms made through their family offices.However,evidence regarding informal venture capital provided to nascent entrepreneurs by small family businesses is scant.I contribute to the literature by modeling the angel investments of about 30,000 family and nonfamily business entrepreneurs across 49 countries,who contributed about$64 million to businesses started by other entrepreneurs over a 3-year period.Based on this sample,I conclude that in countries with higher rates of small family businesses,angel investors are likely fewer,and the amounts invested in start-ups are also lower.Furthermore,I conclude that the owners/managers of small family businesses invest comparatively less capital in start-ups,irrespective of family relationships.I infer that this is attributable to the financing preferences of family businesses,which favor the use of internal resources for their continuity and the growth of their family capital.
文摘Published:25 September 2025 In the article“MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2”(BIOCELL,2021,Vol.45,No.3,pp.627-638.doi:10.32604/biocell.2021.013496),there is an error in the Fig.3.In order to ensure the scientific and rigorous nature of our academic publications,we made a correction about Fig.3 correspondingly.
基金Undergraduate Innovation and Entrepreneurship Training Program,Guangzhou Xinhua University(Project No.:2413092083)。
文摘Objective:To understand the current status and influencing factors of family caregiving for infants and toddlers aged 6–36 months in Tianhe District,Guangzhou City,with the aim of better comprehending the physiological and psychological needs of infants and toddlers,and assisting families in developing effective intervention measures and support strategies.Methods:In November 2024,a random sample of 311 infants and toddlers aged 6–36 months from the permanent resident population in Tianhe District,Guangzhou City,was selected.Caregivers were surveyed using a questionnaire regarding family caregiving practices for infants and toddlers.The Kruskal-Wallis’s test,Mann-Whitney test,and multiple linear regression analysis were employed to examine the influencing factors of family caregiving for infants and toddlers.Results:The findings indicate that the quality of family caregiving is significantly influenced by the educational level of the parents(p<0.001)and the annual household income(p<0.001).Work-family conflict showed a significant negative correlation with the quality of family caregiving(β=-0.239,p<0.001),while family atmosphere demonstrated a significant positive correlation with the quality of family caregiving(β=0.225,p<0.001).Conclusion:In Tianhe District,Guangzhou City,the quality of infant and toddler caregiving is determined by a combination of key factors,including annual household income,parental educational background,work-family conflict,and family atmosphere.These elements collectively form the core determinants of caregiving practices for infants and toddlers.
基金Natural Science Foundation of Shandong Province,No.ZR2020QH185Scientific Research Nurturing Fund of The First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,No.QYPY2020NSFC0803+2 种基金Guangxi Zhuang Autonomous Region Health Commission Scientific Research Project,No.Z-A20220415Guangxi Medical University Teacher Teaching Ability Development Project,No.2022JFA02Guangxi Medical University Undergraduate Education and Teaching Reform Project,No.2023Y05.
文摘BACKGROUND Colorectal cancer(CRC)is the third most common cancer globally,causing over 900000 deaths annually.Risk factors include aging,diet,obesity,sedentary lifestyle,tobacco use,genetic predisposition,and inflammatory bowel disease.Despite current treatments,survival rates for advanced CRC remain low,highlighting the need for better therapeutic strategies.AIM To evaluate both the clinical significance and the pathological implications of the Kinesin family member 14(KIF14)expression within CRC specimens.Additionally,this study aims to investigate the interaction between nitidine chloride(NC)and KIF14,considering their potential as therapeutic targets.METHODS The expression of the KIF14 protein in CRC was analyzed using immunohistochemical staining.The integration of multicenter high-throughput data facilitated the calculation of the standardized mean difference(SMD)for KIF14 mRNA levels.The assessment of clinical and pathological impact was enhanced by analyzing combined receiver operating characteristic curves,along with measures of sensitivity,specificity,and likelihood ratios.Additionally,clustered regularly interspaced short palindromic repeats knockout screening for cell growth and single-cell sequencing were employed to validate the significance of KIF14 expression in CRC.Survival analysis established the prognostic value of KIF14 in CRC.The molecular mechanism of NC against CRC was elucidated through whole-genome sequencing and enrichment analysis,and molecular docking was utilized to explore the targeting affinity between NC and KIF14.RESULTS KIF14 was highly expressed in 208 CRC patients.Data from 17 platforms involving 2436 CRC samples and 1320 noncancerous colorectal tissue controls indicated that KIF14 expression was significantly higher in CRC samples,with an SMD of 1.92(95%CI:1.49-2.35).The area under the curve was 0.94(95%CI:0.92-0.96),with a sensitivity of 0.85(95%CI:0.78-0.90)and a specificity of 0.90(95%CI:0.85-0.93).The positive and negative likelihood ratios were 8.38(95%CI:5.39-13.02)and 0.17(95%CI:0.11-0.26),respectively.At the single-cell level,significant overexpression of KIF14 was observed in CRC cells(P<0.001),with 35 CRC cell lines dependent on KIF14 for growth.The K-M plots demonstrated that KIF14 possesses prognostic value in CRC patients within the GSE71187 and GSE103679 datasets(P<0.05).Binding energy calculations indicated that KIF14 is a potential target for NC(binding energy:10.3 kcal/mol).CONCLUSION KIF14 promotes the growth of CRC cells and acts as an oncogenic factor,potentially serving as a therapeutic target for NC in the treatment of CRC.
基金Supported by the 2023 Government-funded Project of the Outstanding Talents Training Program in Clinical Medicine,No.ZF2023165Key Research and Development Projects of Hebei Province,No.18277731D+1 种基金Natural Science Foundation of Hebei Province,No.H202423105Hebei Provincial Administration of Traditional Chinese Medicine,Scientific Research Project,No.2020014.
文摘In this editorial,we comment on the article by Qin et al,recently published in the World Journal of Gastrointestinal Oncology.Malignant tumors of the digestive tract represent a significant health threat.Kinesin family member 14(KIF14),a critical kinesin,is pivotal in the proliferation,migration,and invasion of tumor cells.It has emerged as a focal point in recent studies of malignant tumors in the digestive tract.This article reviews the current research on KIF14 within these tumors and details its significant role in tumor cell behaviors,including proliferation,apo-ptosis,migration,invasion,and angiogenesis,alongside the regulatory mechanisms of the associated intracellular signaling pathways.Additionally,it explores the clinical value of KIF14 as a potential biomarker for early diagnosis,disease monitoring,and prognostic evaluation in malignant tumors of the digestive tract.The article concludes by introducing the potential regulatory role of traditional Chinese medicine,aiming to combine the strengths of both modern and traditional medical approaches to enhance treatment outcomes and prognosis for patients with these tumors.
基金supported by the Key-Area Research and Development Program of Guangdong Province(Grant No.2020B020220003)National Natural Science Foundation of China(32202504)+2 种基金Guangdong Basic and Applied Basic Research Foundation(Grant No.2023A1515030049)Guangdong Rural Revitalization Strategy Special Project(Grant No.2023-NJS-00-003)Special fund for scientific and technological talents introduction of Guangdong Academy of Agricultural Sciences(Grant No.R2021YJ-YB2004)。
文摘Wax gourd(Benincasa hispida)is an important cucurbit crop with economic and medicinal value.The myeloblastosis(MYB)gene family is one of the largest gene families in plants and regulates various biological processes,whereas the MYB gene family has not been systematically studied in wax gourd.In this study,we performed genome-wide identification of the MYB gene family in wax gourd and analyzed their phylogenetic relationship,MYB DNA-binding domain(MYB DBD),gene structure,protein motif,synteny,duplication mode and expression pattern.As a result,a total of 215 BhMYB genes(BhMYBs)were identified,belonging to four subfamilies:1R-,2R-,3R-and 4R-MYB subfamilies.Genes of 1R-MYB subfamily and 2R-MYB subfamily were subdivided into different subgroups respectively.The analysis of MYB DBD,gene structure and protein motif showed that the most genes in the same subgroup had similar characteristics and the 2R-MYB genes were more conserved than the 1R-MYB genes.Interestingly,the long terminal retrotransposons(LTR-RTs)were found in the long introns of several BhMYBs.The results of synteny analysis showed that there were more syntenic gene pairs between wax gourd and other cucurbit crops,while the least number of syntenic gene pairs existed between wax gourd and rice.Gene duplication was the main reason for the expansion of the MYB gene family in wax gourd,with the transposed duplication(TRD)mode contributing more.All duplication BhMYB genes were under purifying selection pressure.Further expression analysis showed that many BhMYBs exhibited obvious tissue-specific expression and several BhMYBs were significantly induced by one or more abiotic stresses.BhMYB79 was particularly expressed in roots and significantly induced by salt,drought,cold and heat stresses,overexpression of which led to reduced tolerance to salt stress in Arabidopsis.In conclusion,our results provide a systematic analysis of wax gourd MYB gene family and facilitate the biological role study of BhMYB79 during wax gourd salt stress response process.
文摘The analysis of the historical and theoretical origins of the dual identity of Chinese women based on the Marxist view of family-society enables a more scientific and reasonable explanation to the unity of history and logic of Chinese women’s dual identity.Under the guidance of the Marxist concept of family-society,Chinese women will achieve development in both family space and social space;meanwhile,Chinese women in the dual identity development carry the home-state emotion different from that of western women’s;cultivating women’s awareness of family identity is of great significance to the construction of family style or national style in the new era of socialism with Chinese characteristics.
文摘I have a wonderful family.We all like music very much.My mother is a music teacher.She is good at playing the piano and the violin.She also likes singing and she can sing many songs.My father is a doctor.He likes music,too.After he comes back from work,he often listens to music.In fact,playing the flute and the erhu are his favorites.I enjoy playing the guitar though it's difficult to play.I practice playing it every day,so I can play it very well.I once won the second prize at a guitar concert in the city.But my sister thinks playing theguitar is boring.
文摘Hi! I'm Linda.I'm 12 years old.I'm a student.I'm in No.1 middle school(1).Here is a nice photo of my family.These are my parents,Tom and Mary.My mother is a(2)English teacher.My father is a doctor.Those are my brother and sister.My sister(3)name is Helen.She is 6 years old.My brother is Eric.
文摘一、请根据录音,填写单词。二、听对话,选择正确的答案。1.Who is coming for dinner?A.Friends.B.Aunt and uncle.C.Grandma and grandpa.2.What does Dad offer to do at the beginning?A.Go shopping.B.Set the table.C.Cook the fish.
文摘Objective:Cardiopulmonary resuscitation(CPR)is one of the most important life-saving procedures in the hospital.Contrary to medical guidelines,family presence during CPR is still not accepted in some countries.Family presence during CPR depends on the nurses’attitude,which is influenced by various factors.Emotional intelligence(EI)helps nurses make wise decisions and display responsible behavior,which is necessary for proper and good performance in nursing.The study’s purpose was to determine the attitude of acute care nurses toward family presence during CPR and its relationship with EI.Methods:The descriptive-analytical study was conducted among the nurses of acute care units(intensive care unit[ICU],critical care unit,and emergency department)in teaching hospitals of Qazvin University of Medical Sciences,Iran,in 2022-2023.A total of 186 nurses were included in the study using convenience sampling.The data collection tools were a checklist of demographic characteristics,nurses’attitude toward the presence of family scale,and Siberia Schering’s EI questionnaire.The collected data were analyzed using descriptive and analytical statistics and SPSS software.Results:The mean age of the participants was(32.05±6.93)years.Of 186 nurses participating in the study,127(68.3%)were women and the rest were men.The mean score of attitude was 47.41±9.41(the minimum score of the nurses was 19 and the maximum score was 95).The mean score of EI was 99.27±8.86(the minimum score was 69 and the maximum score was 128).The results of Pearson’s correlation coefficient showed no significant relationship between the total score of attitude and EI of the participants(P=0.588).Data analysis showed that only the self-arousal dimension of EI has a significant relationship with the range of family self-control behaviors in attitude(P=0.037).Conclusions:The results showed that the nurses of acute care units have a positive attitude toward the presence of the family during CPR and have high EI.Although no significant relationship was observed between the 2 variables,paying attention to the influencing factors on the attitude of the nurse toward family presence during CPR in different societies needs more research and investigation.
基金supported by the National Social Science Fund of China(Grant No.22BTY098)with JJY as Principal Investigator.
文摘Background:Physical inactivity among adolescents has become a global public health challenge,with over 80%failing to meet the recommendations of the WHO for activity levels.Existing research predominantly examines how physical activity(PA)enhances resilience,while the predictive role of resilience in PA,particularly its interaction with family factors,has received limited attention.This study aimed to examine the associations between resilience and PA among adolescents,focusing on family functioning and gender differences.Methods:In this cross-sectional study,a total of 909 Chinese adolescents(463 males and 446 females,aged 13.30.5 years)completed the±following validated self-report instruments:the Connor-Davidson Resilience Scale with 10 items,the Physical Activity Questionnaire for Older Children,and the Family Adaptability and Cohesion Evaluation Scale II-Chinese version that was used to categorize family functioning into three types(i.e.,lower,balanced,and higher).The generalized linear mixed-effect model(GLMM)was used to determine the contribution of resilience and its interaction with family functioning type on PA after adjusting for age and gender.Results:Males presented significantly higher resilience and PA levels(both p 0.001)as well as better family functioning(p 0.01)than females.Compared with the lower<<functioning group,participants from higher-functioning families showed superior resilience and PA(both p 0.001).<The GLMM analysis revealed a positive relationship between resilience and PA(p 0.001),where the lower functioning<group was significantly weaker than the higher functioning group.Conclusion:Resilience and PA in adolescents vary across gender and family functioning type,with males and adolescents from better-functioning families outperforming their peers.Resilience is a positive predictor of PA in adolescents,with family functioning type being a crucial moderator of such a relationship.
