通过探索生态农产品“O2O+F2F”(Online to Offline+Farm to Family)模式助推嘉兴乡村振兴的产业兴旺,主要对嘉兴5县2区农村生态农产品电子商务发展现状进行深入调研及分析,挖掘出制约嘉兴农村生态农产品电子商务销售的瓶颈,提出相应的...通过探索生态农产品“O2O+F2F”(Online to Offline+Farm to Family)模式助推嘉兴乡村振兴的产业兴旺,主要对嘉兴5县2区农村生态农产品电子商务发展现状进行深入调研及分析,挖掘出制约嘉兴农村生态农产品电子商务销售的瓶颈,提出相应的解决方案,供各美丽乡村和相关部门人员及科研人员参考。展开更多
F2F(Farm to Family)模式是一种生态型都市农业中生产者与消费者相互动的模式。基于F2F模式,农业众筹演变成依托互联网众筹平台,直接将众多小额投资连接到一起的新型农业融资方式。本文从农户的角度,针对所提出的F2F模式特征、农户自身...F2F(Farm to Family)模式是一种生态型都市农业中生产者与消费者相互动的模式。基于F2F模式,农业众筹演变成依托互联网众筹平台,直接将众多小额投资连接到一起的新型农业融资方式。本文从农户的角度,针对所提出的F2F模式特征、农户自身特性及其参与该模式的意愿行为,采用logistic模型探讨了消费者参与该模式的影响因素。结论表明:农民盈利预期以及所能够接受的订单违约率、最高违约金均会显著影响农民对该模式的参与意愿。因此,该模式的实施需要充分考虑农户特征以及F2F模式的自身特点。展开更多
Objective:MicroRNAs(miRNAs)are small,non-coding RNAs that play a key role in the development of chemoresistance in various cancer types,including colorectal cancer(CRC).In this study,we aimed to study the underlying m...Objective:MicroRNAs(miRNAs)are small,non-coding RNAs that play a key role in the development of chemoresistance in various cancer types,including colorectal cancer(CRC).In this study,we aimed to study the underlying mechanisms of miRNA in chemotherapy-resistant CRC.Methods:LoVo CRC cell line was exposed to oxaliplatin at an increased dose,and cells were cultured in the presence of oxaliplatin to develop LoVo^(OXR) cells.Microarray and Quantitative Reverse Transcription Polymerase Chain Reaction(qRT-PCR),western blot,and transwell assay were used to evaluate the chemoresistance in LoVo^(OXR) CRC cells.Results:Microarray and qRT-PCR analysis showed an increased expression of miR-100-5p in LoVo^(OXR) cells.MTT assay and flow cytometry analysis revealed less apoptosis and higher cell viability in LoVo^(OXR) cells.mRNA prediction target gene analysis showed C-terminal domain small phosphatase-like(CTDSPL),a phosphatase-like tumor suppressor,as a key target of miR-100-5p.CTDSPL expression was low in LoVo^(OXR) cells compared to LoVoWT cells.miR-100-5p regulates G1/S and S-phase transitions and inhibits differentiation by targeting the CTDSPL/pRB/E2F1 signaling pathway,which involves the modulation of cell cycle effectors in LoVo^(OXR) cells.Further,we found that forkhead box P3(FOXP3),as the upstream target of miR-100-5p,is highly expressed in LoVo^(OXR) cells.Inhibiting miR-100-5p and FOXP3 down-regulates miR-100-5p expression,while increased CTDSPL expression contributed to reduced cell proliferation and promoted cell apoptosis in LoVo^(OXR) CRC cells.Conclusions:miR-100-5p plays an oncogenic role in inducing chemoresistance through modulation of the CTDSPL/retinoblastoma protein(pRB)/E2F transcription factor 1(E2F1)axis in CRC cells.展开更多
文摘通过探索生态农产品“O2O+F2F”(Online to Offline+Farm to Family)模式助推嘉兴乡村振兴的产业兴旺,主要对嘉兴5县2区农村生态农产品电子商务发展现状进行深入调研及分析,挖掘出制约嘉兴农村生态农产品电子商务销售的瓶颈,提出相应的解决方案,供各美丽乡村和相关部门人员及科研人员参考。
文摘F2F(Farm to Family)模式是一种生态型都市农业中生产者与消费者相互动的模式。基于F2F模式,农业众筹演变成依托互联网众筹平台,直接将众多小额投资连接到一起的新型农业融资方式。本文从农户的角度,针对所提出的F2F模式特征、农户自身特性及其参与该模式的意愿行为,采用logistic模型探讨了消费者参与该模式的影响因素。结论表明:农民盈利预期以及所能够接受的订单违约率、最高违约金均会显著影响农民对该模式的参与意愿。因此,该模式的实施需要充分考虑农户特征以及F2F模式的自身特点。
基金supported by the China Medical University Hospital,Department of Medical Research(DMR-110-209)the Ministry of Science and Technology,Taiwan(MOST:109-2320-B-303-001-MY2),CMU107-ASIA-08,CMU109-MF-76 and CMU105-ASIA-01Taichung Armed Forces General Hospital:TCAFGH_D_115034.
文摘Objective:MicroRNAs(miRNAs)are small,non-coding RNAs that play a key role in the development of chemoresistance in various cancer types,including colorectal cancer(CRC).In this study,we aimed to study the underlying mechanisms of miRNA in chemotherapy-resistant CRC.Methods:LoVo CRC cell line was exposed to oxaliplatin at an increased dose,and cells were cultured in the presence of oxaliplatin to develop LoVo^(OXR) cells.Microarray and Quantitative Reverse Transcription Polymerase Chain Reaction(qRT-PCR),western blot,and transwell assay were used to evaluate the chemoresistance in LoVo^(OXR) CRC cells.Results:Microarray and qRT-PCR analysis showed an increased expression of miR-100-5p in LoVo^(OXR) cells.MTT assay and flow cytometry analysis revealed less apoptosis and higher cell viability in LoVo^(OXR) cells.mRNA prediction target gene analysis showed C-terminal domain small phosphatase-like(CTDSPL),a phosphatase-like tumor suppressor,as a key target of miR-100-5p.CTDSPL expression was low in LoVo^(OXR) cells compared to LoVoWT cells.miR-100-5p regulates G1/S and S-phase transitions and inhibits differentiation by targeting the CTDSPL/pRB/E2F1 signaling pathway,which involves the modulation of cell cycle effectors in LoVo^(OXR) cells.Further,we found that forkhead box P3(FOXP3),as the upstream target of miR-100-5p,is highly expressed in LoVo^(OXR) cells.Inhibiting miR-100-5p and FOXP3 down-regulates miR-100-5p expression,while increased CTDSPL expression contributed to reduced cell proliferation and promoted cell apoptosis in LoVo^(OXR) CRC cells.Conclusions:miR-100-5p plays an oncogenic role in inducing chemoresistance through modulation of the CTDSPL/retinoblastoma protein(pRB)/E2F transcription factor 1(E2F1)axis in CRC cells.
