Wheat powdery mildew(Blumeria graminis f.sp.tritici) is the one of main wheat diseases in China.Based on the internal transcribed spacer(ITS) sequences of ribosome of B.graminis f.sp.tritici,three molecular primer pai...Wheat powdery mildew(Blumeria graminis f.sp.tritici) is the one of main wheat diseases in China.Based on the internal transcribed spacer(ITS) sequences of ribosome of B.graminis f.sp.tritici,three molecular primer pairs(F1/R,F2/R and F3/R) were designed to detect the fungal pathogen of wheat powdery mildew.The species specificity of these primers was confirmed.F1/R was demonstrated a higher sensitivity than the other two primer pairs,and could detect as low as 1 pg DNA of B.graminis f.sp.tritici.Furthermore,F1/R primer pair was used to detect the pathogen DNA extracted from wheat leaves showing chlorosis and typical symptoms of powdery mildew caused by artificial inoculation with B.graminis f.sp.tritici.The preliminary results demonstrated the usefulness of this primer pair and its potential applications in efficient detection of wheat powdery mildew pathogen from leaves with latent infections at early growth stages of wheat.展开更多
对新近分离的鹅副粘病毒SF0 2株采用RT PCR方法 ,扩增部分F基因进行测序 ,其裂解位点的序列为112 R R Q K R F117,与新城疫病毒强毒株的特征相符。通过对其F基因与我国大陆新城疫强毒F4 8E9株、疫苗毒Lasota株比较 ,发现该毒株的F基因...对新近分离的鹅副粘病毒SF0 2株采用RT PCR方法 ,扩增部分F基因进行测序 ,其裂解位点的序列为112 R R Q K R F117,与新城疫病毒强毒株的特征相符。通过对其F基因与我国大陆新城疫强毒F4 8E9株、疫苗毒Lasota株比较 ,发现该毒株的F基因已发生了较大的变异 ,而与1999年在我国台湾省流行的新城疫病毒基因Ⅶ型有很高的同源性。展开更多
文摘Wheat powdery mildew(Blumeria graminis f.sp.tritici) is the one of main wheat diseases in China.Based on the internal transcribed spacer(ITS) sequences of ribosome of B.graminis f.sp.tritici,three molecular primer pairs(F1/R,F2/R and F3/R) were designed to detect the fungal pathogen of wheat powdery mildew.The species specificity of these primers was confirmed.F1/R was demonstrated a higher sensitivity than the other two primer pairs,and could detect as low as 1 pg DNA of B.graminis f.sp.tritici.Furthermore,F1/R primer pair was used to detect the pathogen DNA extracted from wheat leaves showing chlorosis and typical symptoms of powdery mildew caused by artificial inoculation with B.graminis f.sp.tritici.The preliminary results demonstrated the usefulness of this primer pair and its potential applications in efficient detection of wheat powdery mildew pathogen from leaves with latent infections at early growth stages of wheat.
文摘对新近分离的鹅副粘病毒SF0 2株采用RT PCR方法 ,扩增部分F基因进行测序 ,其裂解位点的序列为112 R R Q K R F117,与新城疫病毒强毒株的特征相符。通过对其F基因与我国大陆新城疫强毒F4 8E9株、疫苗毒Lasota株比较 ,发现该毒株的F基因已发生了较大的变异 ,而与1999年在我国台湾省流行的新城疫病毒基因Ⅶ型有很高的同源性。
