Via multi-dimensional gas chromatography, configured with parallel dual-channel, double detectors, valves switching and back flushing, rapid analysis of the gas compositions consisting of C1-C5 hydrocarbons and perman...Via multi-dimensional gas chromatography, configured with parallel dual-channel, double detectors, valves switching and back flushing, rapid analysis of the gas compositions consisting of C1-C5 hydrocarbons and permanent gases, such as CO2, H2S, H2, and CO, for direct coal liquefaction has been realized. With four packed chromatographic columns, which are Hayesep-Q pre-column, Hayesep-Q column, molecular sieve 5A column and one PLOT A1203 S capillary column, the gas compositions for direct coal liquefaction are analyzed qualitatively and quantitatively by the external standard method. The determination method has such advantages as excellent separation, simple operation, rapid analysis and accurate results.展开更多
Objective: to establish multi-components by single marker (QAMS) and HPlC method for the determination of four substances (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin and to provide experimental basis ...Objective: to establish multi-components by single marker (QAMS) and HPlC method for the determination of four substances (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin and to provide experimental basis for the quality standard of Compound Yizhihao Granules. Methods: the sample treatment method was improved, and HPLC external standard method was established for the determination of four substances in Compound Yizhihao Granules. The chromatographic column was Wondersil C18 (4.6mm×250mm, 5μm), the flow rate was 1.0mL/min, the check wavelength was 245nm, the column temperature was 30℃, the sample was 20 μL, the mobile phase was acetonitrile-water. The relative correction factors (RCF) of (R, S) - Epigoitrin, Vitexicarpin, Artemisetin were determined with Indirubin as internal reference. The RCF was used to calculate the content of the substance under test, and then the content of the substance under test was determined by the external standard method to verify the feasibility of the multi-components by single marker. Results: the treatment method of the test sample was as follows: solid-liquid ratio was 1:5. Ultrasound was 45 min, trichloromethane extraction, and steam dry concentration with methanol constant volume. The results showed that the good linear ranges of (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin were 5.15-103 μ g / mL, 5.005-100.1 μ g / mL, 0.5-10 μ g / mL and 0.5115-10.23 μ g / mL, respectively. The average recoveries were 95.19%, 95.73%, 90.75%, 91.65%. The relative correction factors (RCF) of (R, S) - Epigoitrin, Vitexicarpin, Artemisetin were determined with Indirubin as internal reference was 0.2%, 0.6% and 0.3%. And the relative error of multi-components by single marker and external standard method was less than 1%. Conclusion: the multi-components by single marker method (QAMS) is simple, stable and can be used for the determination of multiple substances in Compound Yizhihao Granules.展开更多
文摘Via multi-dimensional gas chromatography, configured with parallel dual-channel, double detectors, valves switching and back flushing, rapid analysis of the gas compositions consisting of C1-C5 hydrocarbons and permanent gases, such as CO2, H2S, H2, and CO, for direct coal liquefaction has been realized. With four packed chromatographic columns, which are Hayesep-Q pre-column, Hayesep-Q column, molecular sieve 5A column and one PLOT A1203 S capillary column, the gas compositions for direct coal liquefaction are analyzed qualitatively and quantitatively by the external standard method. The determination method has such advantages as excellent separation, simple operation, rapid analysis and accurate results.
文摘Objective: to establish multi-components by single marker (QAMS) and HPlC method for the determination of four substances (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin and to provide experimental basis for the quality standard of Compound Yizhihao Granules. Methods: the sample treatment method was improved, and HPLC external standard method was established for the determination of four substances in Compound Yizhihao Granules. The chromatographic column was Wondersil C18 (4.6mm×250mm, 5μm), the flow rate was 1.0mL/min, the check wavelength was 245nm, the column temperature was 30℃, the sample was 20 μL, the mobile phase was acetonitrile-water. The relative correction factors (RCF) of (R, S) - Epigoitrin, Vitexicarpin, Artemisetin were determined with Indirubin as internal reference. The RCF was used to calculate the content of the substance under test, and then the content of the substance under test was determined by the external standard method to verify the feasibility of the multi-components by single marker. Results: the treatment method of the test sample was as follows: solid-liquid ratio was 1:5. Ultrasound was 45 min, trichloromethane extraction, and steam dry concentration with methanol constant volume. The results showed that the good linear ranges of (R, S) - Epigoitrin, Vitexicarpin, Indirubin and Artemisetin were 5.15-103 μ g / mL, 5.005-100.1 μ g / mL, 0.5-10 μ g / mL and 0.5115-10.23 μ g / mL, respectively. The average recoveries were 95.19%, 95.73%, 90.75%, 91.65%. The relative correction factors (RCF) of (R, S) - Epigoitrin, Vitexicarpin, Artemisetin were determined with Indirubin as internal reference was 0.2%, 0.6% and 0.3%. And the relative error of multi-components by single marker and external standard method was less than 1%. Conclusion: the multi-components by single marker method (QAMS) is simple, stable and can be used for the determination of multiple substances in Compound Yizhihao Granules.
