OBJECTIVE:To explore the potential molecular mechanism of Qigu capsule(芪骨胶囊,QGC) in the treatment of sarcopenia through network pharmacology and to verify it experimentally.METHODS:The active compounds of QGC and ...OBJECTIVE:To explore the potential molecular mechanism of Qigu capsule(芪骨胶囊,QGC) in the treatment of sarcopenia through network pharmacology and to verify it experimentally.METHODS:The active compounds of QGC and common targets between QGC and sarcopenia were screened from databases.Then the herbs-compounds-targets network,and protein-protein interaction(PPI) network was constructed.Gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed by R software.Next,we used a dexamethasone-induced sarcopenia mouse model to evaluate the anti-sarcopenic mechanism of QGC.RESULTS:A total of 57 common targets of QGC and sarcopenia were obtained.Based on the enrichment analysis of GO and KEGG,we took the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt) signaling pathway as a key target to explore the mechanism of QGC on sarcopenia.Animal experiments showed that QGC could increase muscle strength and inhibit muscle fiber atrophy.In the model group,the expression of muscle ring finger-1 and Atrogin-1 were increased,while myosin heavy chain was decreased,QGC treatment reversed these changes.Moreover,compared with the model group,the expressions of pPI3K,p-Akt,p-mammalian target of rapamycin and pForkhead box O3 in the QGC group were all upregulated.CONCLUSION:QGC exerts an anti-sarcopenic effect by activating PI3K/Akt signaling pathway to regulate skeletal muscle protein metabolism.展开更多
Objective Forsythia suspensa has long been utilized in traditional Chinese medicine(TCM)for the treatment of IgA nephropathy(IgAN),the most prevalent form of primary glomerular disease.However,the precise mechanisms r...Objective Forsythia suspensa has long been utilized in traditional Chinese medicine(TCM)for the treatment of IgA nephropathy(IgAN),the most prevalent form of primary glomerular disease.However,the precise mechanisms remain inadequately understood.This study seeks to elucidate the underlying mechanisms of Forsythia suspensa extract(FSE)in the treatment of IgAN by employing an integrated approach that combines network pharmacology with in vivo experimental validation.Methods The chemical components of FSE were identified using high-performance liquid chromatography-mass spectrometry(HPLC–MS/MS).Additional chemical components and targets were determined through the Traditional Chinese Medicine Systems Pharmacology database.Potential therapeutic targets for IgAN were sourced from GeneCards and the Comparative Toxicogenomics Database.Subsequently,the enrichment analyses were conducted to evaluate the biological functions and pathways associated with the core targets.Finally,a mousemodel of IgAN was developed to validate the findings of the network pharmacology analysis.Results Through network analysis and HPLC–MS/MS,31 chemical components of FSE were identified.A total of 99 common targets were discovered between FSE and IgAN.The enrichment analyses suggested that FSE may mitigate IgAN primarily by inhibiting the TLR and NF-κB signaling pathways.In vivo experiments demonstrated that FSE reduced inflammation and preserved renal function in mice with IgAN through the Tolllike receptor 9(TLR9)/NF-κB pathway.Conclusion The integration of network pharmacology and animal experiments suggests that FSE alleviates renal inflammation and damage in IgAN through the TLR9/NF-κB signaling pathway.展开更多
Traditional Medicine Research published an article entitled Investigation on the mechanism of Qi-Hong-Tang-Zu ointment in thetreatmentofdiabetic wound by network pharmacology and experimental validation on 24 January ...Traditional Medicine Research published an article entitled Investigation on the mechanism of Qi-Hong-Tang-Zu ointment in thetreatmentofdiabetic wound by network pharmacology and experimental validation on 24 January 2025.The author confirmed this article’s proof on 23 January 2025 without any questions.But on 06 July 2025,the editorial team found,there was an error in Figure 8.Specifically,Figure 8E,Figure 8F and Figure 8G were mistakenly duplicated.The authors acknowledge that the original version of Figure 8 was incorrect and have provided the revised,correct version in this corrigendum.The authors would like to assert that there is no change in the body text of the article.展开更多
A tracking stability control problem for the vertical electric stabilization system of moving tank based on adaptive robust servo control is addressed.This paper mainly focuses on two types of possibly fast timevaryin...A tracking stability control problem for the vertical electric stabilization system of moving tank based on adaptive robust servo control is addressed.This paper mainly focuses on two types of possibly fast timevarying but bounded uncertainty within the vertical electric stabilization system:model parameter uncertainty and uncertain nonlinearity.First,the vertical electric stabilization system is constructed as an uncertain nonlinear dynamic system that can reflect the practical mechanics transfer process of the system.Second,the dynamical equation in the form of state space is established by designing the angular tracking error.Third,the comprehensive parameter of system uncertainty is designed to estimate the most conservative effects of uncertainty.Finally,an adaptive robust servo control which can effectively handle the combined effects of complex nonlinearity and uncertainty is proposed.The feasibility of the proposed control strategy under the practical physical condition is validated through the tests on the experimental platform.This paper pioneers the introduction of the internal nonlinearity and uncertainty of the vertical electric stabilization system into the settlement of the tracking stability control problem,and validates the advanced servo control strategy through experiment for the first time.展开更多
To accelerate the practicality of electromagnetic railguns,it is necessary to use a combination of threedimensional numerical simulation and experiments to study the mechanism of bore damage.In this paper,a three-dime...To accelerate the practicality of electromagnetic railguns,it is necessary to use a combination of threedimensional numerical simulation and experiments to study the mechanism of bore damage.In this paper,a three-dimensional numerical model of the augmented railgun with four parallel unconventional rails is introduced to simulate the internal ballistic process and realize the multi-physics field coupling calculation of the rail gun,and a test experiment of a medium-caliber electromagnetic launcher powered by pulse formation network(PFN)is carried out.Various test methods such as spectrometer,fiber grating and high-speed camera are used to test several parameters such as muzzle initial velocity,transient magnetic field strength and stress-strain of rail.Combining the simulation results and experimental data,the damage condition of the contact surface is analyzed.展开更多
BACKGROUND Diabetic kidney disease(DKD)is one of the serious complications of diabetes mellitus,and the existing treatments cannot meet the needs of today's patients.Traditional Chinese medicine has been validated...BACKGROUND Diabetic kidney disease(DKD)is one of the serious complications of diabetes mellitus,and the existing treatments cannot meet the needs of today's patients.Traditional Chinese medicine has been validated for its efficacy in DKD after many years of clinical application.However,the specific mechanism by which it works is still unclear.Elucidating the molecular mechanism of the Nardostachyos Radix et Rhizoma-rhubarb drug pair(NRDP)for the treatment of DKD will provide a new way of thinking for the research and development of new drugs.AIM To investigate the mechanism of the NRDP in DKD by network pharmacology combined with molecular docking,and then verify the initial findings by in vitro experiments.METHODS The Traditional Chinese Medicine Systems Pharmacology(TCMSP)database was used to screen active ingredient targets of NRDP.Targets for DKD were obtained based on the Genecards,OMIM,and TTD databases.The VENNY 2.1 database was used to obtain DKD and NRDP intersection targets and their Venn diagram,and Cytoscape 3.9.0 was used to build a"drug-component-target-disease"network.The String database was used to construct protein interaction networks.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis and Gene Ontology analysis were performed based on the DAVID database.After selecting the targets and the active ingredients,Autodock software was used to perform molecular docking.In experimental validation using renal tubular epithelial cells(TCMK-1),we used the Cell Counting Kit-8 assay to detect the effect of NRDP on cell viability,with glucose solution used to mimic a hyperglycemic environment.Flow cytometry was used to detect the cell cycle progression and apoptosis.Western blot was used to detect the protein expression of STAT3,p-STAT3,BAX,BCL-2,Caspase9,and Caspase3.RESULTS A total of 10 active ingredients and 85 targets with 111 disease-related signaling pathways were obtained for NRDP.Enrichment analysis of KEGG pathways was performed to determine advanced glycation end products(AGEs)-receptor for AGEs(RAGE)signaling as the core pathway.Molecular docking showed good binding between each active ingredient and its core targets.In vitro experiments showed that NRDP inhibited the viability of TCMK-1 cells,blocked cell cycle progression in the G0/G1 phase,and reduced apoptosis in a concentrationdependent manner.Based on the results of Western blot analysis,NRDP differentially downregulated p-STAT3,BAX,Caspase3,and Caspase9 protein levels(P<0.01 or P<0.05).In addition,BAX/BCL-2 and p-STAT3/STAT3 ratios were reduced,while BCL-2 and STAT3 protein expression was upregulated(P<0.01).CONCLUSION NRDP may upregulate BCL-2 and STAT3 protein expression,and downregulate BAX,Caspase3,and Caspase9 protein expression,thus activating the AGE-RAGE signaling pathway,inhibiting the vitality of TCMK-1 cells,reducing their apoptosis.and arresting them in the G0/G1 phase to protect them from damage by high glucose.展开更多
Gastric cancer(GC)remains a formidable global health concern with significant morbidity and mortality rates,despite the fact that numerous advances have been made to improve conventional therapies.Xiaojianzhong decoct...Gastric cancer(GC)remains a formidable global health concern with significant morbidity and mortality rates,despite the fact that numerous advances have been made to improve conventional therapies.Xiaojianzhong decoction(XJZ),a traditional Chinese medicine,has garnered academic attention as a multicomponent,multitarget approach to managing GC.The present editorial explores the potential of XJZ in the treatment of GC through a comprehensive analysis of network pharmacology and experimental validation.Network pharmacology was used to identify key molecular targets of XJZ,including interleukin 6,prostaglandin-endoperoxide synthase 2,and matrix metalloproteinase 9,and in vitro experiments were used to confirm the efficacy of XJZ in inhibiting cell proliferation,inducing apoptosis,and modulating gene expression associated with GC progression.This editorial highlights XJZ as a promising therapeutic strategy for GC and indicates a need for further clinical exploration and validation of its efficacy.展开更多
Focusing on revealing the origin of high ammonia yield rate on Cu via nitrate reduction(NO3RR),we herein applied constant potential method via grand-canonical density functional theory(GC-DFT)with implicit continuum s...Focusing on revealing the origin of high ammonia yield rate on Cu via nitrate reduction(NO3RR),we herein applied constant potential method via grand-canonical density functional theory(GC-DFT)with implicit continuum solvation model to predict the reaction energetics of NO3RR on pure copper surface in alkaline media.The potential-dependent mechanism on the most prevailing Cu(111)and the minor(100)and(110)facets were established,in consideration of NO_(2)_(−),NO,NH_(3),NH_(2)OH,N_(2),and N_(2)O as the main products.The computational results show that the major Cu(111)is the ideal surface to produce ammonia with the highest onset potential at 0.06 V(until−0.37 V)and the highest optimal potential at−0.31 V for ammonia production without kinetic obstacles in activation energies at critical steps.For other minor facets,the secondary Cu(100)shows activity to ammonia from−0.03 to−0.54 V with the ideal potential at−0.50 V,which requires larger overpotential to overcome kinetic activation energy barriers.The least Cu(110)possesses the longest potential range for ammonia yield from−0.27 to−1.12 V due to the higher adsorption coverage of nitrate,but also with higher tendency to generate di-nitrogen species.Experimental evaluations on commercial Cu/C electrocatalyst validated the accuracy of our proposed mechanism.The most influential(111)surface with highest percentage in electrocatalyst determined the trend of ammonia production.In specific,the onset potential of ammonia production at 0.1 V and emergence of yield rate peak at−0.3 V in experiments precisely located in the predicted potentials on Cu(111).Four critical factors for the high ammonia yield and selectivity on Cu surface via NO3RR are summarized,including high NO3RR activity towards ammonia on the dominant Cu(111)facet,more possibilities to produce ammonia along different pathways on each facet,excellent ability for HER inhibition and suitable surface size to suppress di-nitrogen species formation at high nitrate coverage.Overall,our work provides comprehensive potential-dependent insights into the reaction details of NO3RR to ammonia,which can serve as references for the future development of NO3RR electrocatalysts,achieving higher activity and selectivity by maximizing these characteristics of copper-based materials.展开更多
Underwater jet propulsion bio-inspired robots have typically been designed based on soft-bodied organisms, exhibiting relatively limited forms of locomotion. Scallop, a bivalve organism capable of jet propulsion, hold...Underwater jet propulsion bio-inspired robots have typically been designed based on soft-bodied organisms, exhibiting relatively limited forms of locomotion. Scallop, a bivalve organism capable of jet propulsion, holds significant importance in the study of underwater motion mechanisms. In this study, we present theoretical fluid mechanics analysis and modeling of the three distinct motion stages of scallops, providing parameterized descriptions of scallop locomotion mechanisms. Accordingly, three-stage adaptive motion control for the scallop robot and model-based robot configuration optimization design were achieved. An experimental platform and a robot prototype were built to validate the accuracy of the motion model and the effectiveness of the control strategy. Additionally, based on the models, future optimization directions for the robot are proposed.展开更多
3D printing technology can realize the rapid fabrication of complicated structures with short production chain,which just meet the requirements for space manufacturing in the future.This Special Issue features the cut...3D printing technology can realize the rapid fabrication of complicated structures with short production chain,which just meet the requirements for space manufacturing in the future.This Special Issue features the cutting-edge 3D printing technologies considering the space environment,focusing on the experimental validation and simulation on the 3D printing process and structural technologies,including whole process chain from raw materials,structural design,process,equipment,as well as functional verification.展开更多
Lost acceleration response reconstruction is crucial for assessing structural conditions in structural health monitoring(SHM).However,traditional methods struggle to address the reconstruction of acceleration response...Lost acceleration response reconstruction is crucial for assessing structural conditions in structural health monitoring(SHM).However,traditional methods struggle to address the reconstruction of acceleration responses with complex features,resulting in a lower reconstruction accuracy.This paper addresses this challenge by leveraging the advanced feature extraction and learning capabilities of fully convolutional networks(FCN)to achieve precise reconstruction of acceleration responses.In the designed network architecture,the incorporation of skip connections preserves low-level details of the network,greatly facilitating the flow of information and improving training efficiency and accuracy.Dropout techniques are employed to reduce computational load and enhance feature extraction.The proposed FCN model automatically extracts high-level features from the input data and establishes a nonlinearmapping relationship between the input and output responses.Finally,the accuracy of the FCN for structural response reconstructionwas evaluated using acceleration data from an experimental arch rib and comparedwith several traditional methods.Additionally,this approach was applied to reconstruct actual acceleration responses measured by an SHM system on a long-span bridge.Through parameter analysis,the feasibility and accuracy of aspects such as available response positions,the number of available channels,and multi-channel response reconstruction were explored.The results indicate that this method exhibits high-precision response reconstruction capability in both time and frequency domains.,with performance surpassing that of other networks,confirming its effectiveness in reconstructing responses under various sensor data loss scenarios.展开更多
Experimental validation of laser intensity is particularly important for the study of fundamental physics at extremely high intensities.However,reliable diagnosis of the focal spot and peak intensity faces huge challe...Experimental validation of laser intensity is particularly important for the study of fundamental physics at extremely high intensities.However,reliable diagnosis of the focal spot and peak intensity faces huge challenges.In this work,we demonstrate for the firs time that the coherent radiation farfiel patterns from laser–foil interactions can serve as an in situ,real-time,and easy-to-implement diagnostic for an ultraintense laser focus.The laser-driven electron sheets,curved by the spatially varying laser fiel and leaving the targets at nearly the speed of light,produce doughnut-shaped patterns depending on the shapes of the focal spot and the absolute laser intensities.Assisted by particle-in-cell simulations,we can achieve measurements of the intensity and the focal spot,and provide immediate feedback to optimize the focal spots for extremely high intensity.展开更多
Within the SILVARSTAR project,a user-friendly frequency-based hybrid prediction tool has been developed to assess the environmental impact of railway-induced vibration.This tool is integrated in existing noise mapping...Within the SILVARSTAR project,a user-friendly frequency-based hybrid prediction tool has been developed to assess the environmental impact of railway-induced vibration.This tool is integrated in existing noise mapping software.Following modern vibration standards and guidelines,the vibration velocity level in a building in each frequency band is expressed as the sum of a force density(source term),line source transfer mobility(propagation term)and building correction factor(receiver term).A hybrid approach is used that allows for a combination of experimental data and numerical predictions,providing increased flexibility and applicability.The train and track properties can be selected from a database or entered as numerical values.The user can select soil impedance and transfer functions from a database,pre-computed for a wide range of parameters with state-of-the-art models.An experimental database of force densities,transfer functions,free field vibration and input parameters is also provided.The building response is estimated by means of building correction factors.Assumptions within the modelling approach are made to reduce computation time but these can influence prediction accuracy;this is quantified for the case of a nominal intercity train running at different speeds on a ballasted track supported by homogeneous soil of varying stiffness.The paper focuses on the influence of these parameters on the compliance of the track–soil system and the free field response.We also demonstrate the use and discuss the validation of the vibration prediction tool for the case of a high-speed train running on a ballasted track in Lincent(Belgium).展开更多
The train-bridge dynamic interaction problem began with the development of railway technology, and requires an evaluation method for bridge design in order to ensure the safety and stability of the bridge and the runn...The train-bridge dynamic interaction problem began with the development of railway technology, and requires an evaluation method for bridge design in order to ensure the safety and stability of the bridge and the running train. This problem is studied using theoretical analysis, numerical simulation, and experimental study. In the train-bridge dynamic interaction system proposed in this paper, the train vehicle model is established by the rigid-body dynamics method, the bridge model is established by the finite element method, and the wheel/rail vertical and lateral interaction are simulated by the corresponding assumption and the Kalker linear creep theory, respectively. Track irregularity, structure deformation, wind load, collision load, structural damage, foundation scouring, and earthquake action are regarded as the excitation for the system. The train-bridge dynamic interaction system is solved by inter-history iteration. A case study of the dynamic response of a CRH380BL high-speed train running through a standard-design bridge in China is discussed. The dynamic responses of the vehicle and of the bridge subsystems are obtained for speeds ranging from 200 km-b-1 to 400 km.h-1, and the vibration mechanism are analyzed.展开更多
To reduce the surface cracks of extrusion rod for AZ31 magnesium caused by nonhomogeneous metal flow in extrusion process, 3D computer finite element （FE） simulations of extruding a wrought magnesium alloy AZ31 into...To reduce the surface cracks of extrusion rod for AZ31 magnesium caused by nonhomogeneous metal flow in extrusion process, 3D computer finite element （FE） simulations of extruding a wrought magnesium alloy AZ31 into rods have been performed and the results have been verified in extrusion experiments under identical conditions. The tendency to generate the dead zone is decreased by employing the die angle 60° at the cone-shaped die comparing with the die angle 180°. The surface additional tensile stresses of the rod at the die exit are decreased greatly so that the surface cracks caused are avoided by using the die angle 60°. The extrusion die with die angle 180° would increase the higher temperature rise and possibility of crack formation on the rod surface that caused by die angle 60° and temperature rise decrease tensile strength of the AZ31 rod. The experimental results show that die angle 180° could cause continuous cracks on the surface of the extruded rod. The extrusion force required is reduced approximately 15 ton by employing the die angle 60°. Theoretical results obtained by the DeformTM-3D simulation agreed well with the experiments. The obtained results provide the fundamental and also practical guidelines for the design and correction of dies to produce magnesium rod with good surface quality.展开更多
To gain insight into the inelastic behavior of piles, the response of a vertical pile embedded in dry sand and subjected to cyclic lateral loading was studied experimentally in centrifuge tests conducted in Laboratoir...To gain insight into the inelastic behavior of piles, the response of a vertical pile embedded in dry sand and subjected to cyclic lateral loading was studied experimentally in centrifuge tests conducted in Laboratoire Central des Ponts et Chaussees. Three types of cyclic loading were applied, two asymmetric and one symmetric with respect to the unloaded pile. An approximately square-root variation of soil stiffness with depth was obtained from indirect in-flight density measurements, laboratory tests on reconstituted samples, and well-established empirical correlations. The tests were simulated using a cyclic nonlinear Winkler spring model, which describes the full range of inelastic phenomena, including separation and re-attachment of the pile from and to the soil. The model consists of three mathematical expressions capable of reproducing a wide variety of monotonic and cyclic experimentalp-y curves. The physical meaning of key model parameters is graphically explained and related to soil behavior. Comparisons with the centrifuge test results demonstrate the general validity of the model and its ability to capture several features of pile-soil interaction, including： soil plastification at an early stage of loading, ＂pinching＂ behavior due to the formation of a relaxation zone around the upper part of the pile, and stiffness and strength changes due to cyclic loading. A comparison of the p-y curves derived from the test results and the proposed model, as well as those from the classical curves of Reese et al. （1974） for sand, is also presented.展开更多
The current design of hydro-viscous clutch（HVC） in tracked vehicle fan transmission mainly focuses on high-speed and high power. However, the fluid torque under the influence of fluid temperature can not be predicte...The current design of hydro-viscous clutch（HVC） in tracked vehicle fan transmission mainly focuses on high-speed and high power. However, the fluid torque under the influence of fluid temperature can not be predicted accurately by conventional mathematical model or experimental research. In order to validate the fluid torque of HVC by taking the viscosity-temperature characteristic of fluid into account, the test rig is designed. The outlet oil temperature is measured and fitted with different rotation speed, oil film thickness, oil flow rate, and inlet oil temperature. Meanwhile, the film torque can be obtained. Based on Navier-Stokes equations and the continuity equation, the mathematical model of fluid torque is proposed in cylindrical coordinate. Iterative method is employed to solve the equations. The radial and tangential speed distribution, radial pressure distribution and theoretical flow rate are determined and analyzed. The models of equivalent radius and fluid torque of friction pairs are introduced. The experimental and theoretical results indicate that tangential speed distribution is mainly determined by the relative rotating speed between the friction plate and the separator disc. However, the radial speed distribution and pressure distribution are dominated by pressure difference at the lower rotating speed. The oil film fills the clearance and the film torque increases with increasing rotating speed. However, when the speed reaches a certain value, the centrifugal force will play an important role on the fluid distribution. The pressure is negative at the outer radius when inlet flow rate is less than theoretical flow, so the film starts to shrink which decreases the film torque sharply. The theoretical fluid torque has good agreement with the experimental data. This research proposes a new fluid torque mathematical model which may predict the film torque under the influence of temperature more accurately.展开更多
With increasing amount of unconventional natural gas,the production of ethane,propane and other low alkanes continues to increase.In our previous works,a partially decoupled process(PDP) was proposed for conversion of...With increasing amount of unconventional natural gas,the production of ethane,propane and other low alkanes continues to increase.In our previous works,a partially decoupled process(PDP) was proposed for conversion of ethane based on numerical simulations,which showed higher acetylene and ethylene selectivities than the original partial oxidation process.In the current work,the PDP of ethane for producing acetylene and ethylene was studied experimentally to verify the PDP concept.In the PDP of ethane,coke-oven gas or other cheap gas combusts with stoichiometric oxygen as heat carrier,and ethane is mixed with the heat carrier and undergoes pyrolysis at high temperatures.The jet-in-cross-flow(JICF) reactor was designed and manufactured to realize the PDP.A positioning device of 0.1 mm accuracy and a mass spectrometer were used to measure the spatial profiles of the species concentrations.The maximum combined yield(52.7%) of acetylene and ethylene was obtained even at the condition of heat loss,confirming that the PDP of ethane was advantageous over the partial oxidation process and at least comparable to the steam cracking process.展开更多
This paper aims to present the configuration design approach and the energy management strategy （EMS） of a series-parallel hybrid electric transit bus （SPHEB） jointly developed by Shanghai Automotive Industry Co. ...This paper aims to present the configuration design approach and the energy management strategy （EMS） of a series-parallel hybrid electric transit bus （SPHEB） jointly developed by Shanghai Automotive Industry Co. Ltd. （SAIC） and Shanghai Jiao Tong University （SJTU）, China. A major feature of this SPHEB is that a novel manual transmission is designed to switch the powertrain configuration between series and parallel types. To reduce the fuel consumption as well as sustain the battery state of charge, an EMS including seven energy flow modes is designed and applied to this SPHEB. Governed by this EMS, the engine is maintained to operate in high efficiency regions. The experimental test carded on the transit bus city driving cycle is described and analyzed. The experimental results demonstrate the technical feasibility and fuel economy of this approach.展开更多
MicroRNAs （miRNAs） are a group of regulatory RNAs that regulate gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs （mRNAs）. Regulation is accomplis...MicroRNAs （miRNAs） are a group of regulatory RNAs that regulate gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs （mRNAs）. Regulation is accomplished when the 22-25 nucleotide miRNAs bind to complementary sequences in the 3＇-untranslated regions （UTR）. One barrier to miRNA research is to find target genes. Although computational target predictions have shed light on important aspects of microRNA target recognition, questions remain concerning the rates of false positives. In addition, we do not completely understand how microRNAs can recognize and regulate their targets. As such, experimental positive predictions and allow for an unbiased stu ap dy proaches are required, which can reflect in vivo processes, eliminating false of microRNA target recognition. In this review, we summarized experimental approaches that have been described for the identification and validation of mRNA targets associated with specific miRNAs.展开更多
基金Shanghai Clinical Research Center for Chronic Musculoskeletal Diseases (20MC1920600)Shanghai Key Clinical Specialty "Traditional Chinese Medicine Orthopaedic Traumatology"(shslczdzk03901)+3 种基金The Second Round of Construction Project of National TCM Academic School Inheritance Studio "Shi's Trauma Department"[Letter of the People's Education of Traditional Chinese Medicine (2019) No.62]Shanghai High-level Local Universities "Chronic Muscle and Bone Damage Research and Transformation" Innovation Team [No.3 of Shanghai Education Commission (2022)]Program for Shanghai High-Level Local University Innovation Team (SZY20220315)Shanghai Shenkang Hospital Development Center Clinical Three-year Action Plan (SHDC2020CR3090B)。
文摘OBJECTIVE:To explore the potential molecular mechanism of Qigu capsule(芪骨胶囊,QGC) in the treatment of sarcopenia through network pharmacology and to verify it experimentally.METHODS:The active compounds of QGC and common targets between QGC and sarcopenia were screened from databases.Then the herbs-compounds-targets network,and protein-protein interaction(PPI) network was constructed.Gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis were performed by R software.Next,we used a dexamethasone-induced sarcopenia mouse model to evaluate the anti-sarcopenic mechanism of QGC.RESULTS:A total of 57 common targets of QGC and sarcopenia were obtained.Based on the enrichment analysis of GO and KEGG,we took the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt) signaling pathway as a key target to explore the mechanism of QGC on sarcopenia.Animal experiments showed that QGC could increase muscle strength and inhibit muscle fiber atrophy.In the model group,the expression of muscle ring finger-1 and Atrogin-1 were increased,while myosin heavy chain was decreased,QGC treatment reversed these changes.Moreover,compared with the model group,the expressions of pPI3K,p-Akt,p-mammalian target of rapamycin and pForkhead box O3 in the QGC group were all upregulated.CONCLUSION:QGC exerts an anti-sarcopenic effect by activating PI3K/Akt signaling pathway to regulate skeletal muscle protein metabolism.
基金supported by the Natural Science Foundation of China(82560923)Natural Science Foundation of InnerMongolia(2019MS08008)+1 种基金Natural Science Foundation of Inner Mongolia Joint Program(2023LHMS08075)General Project of Inner Mongolia Medical University(YKD2025MS026).
文摘Objective Forsythia suspensa has long been utilized in traditional Chinese medicine(TCM)for the treatment of IgA nephropathy(IgAN),the most prevalent form of primary glomerular disease.However,the precise mechanisms remain inadequately understood.This study seeks to elucidate the underlying mechanisms of Forsythia suspensa extract(FSE)in the treatment of IgAN by employing an integrated approach that combines network pharmacology with in vivo experimental validation.Methods The chemical components of FSE were identified using high-performance liquid chromatography-mass spectrometry(HPLC–MS/MS).Additional chemical components and targets were determined through the Traditional Chinese Medicine Systems Pharmacology database.Potential therapeutic targets for IgAN were sourced from GeneCards and the Comparative Toxicogenomics Database.Subsequently,the enrichment analyses were conducted to evaluate the biological functions and pathways associated with the core targets.Finally,a mousemodel of IgAN was developed to validate the findings of the network pharmacology analysis.Results Through network analysis and HPLC–MS/MS,31 chemical components of FSE were identified.A total of 99 common targets were discovered between FSE and IgAN.The enrichment analyses suggested that FSE may mitigate IgAN primarily by inhibiting the TLR and NF-κB signaling pathways.In vivo experiments demonstrated that FSE reduced inflammation and preserved renal function in mice with IgAN through the Tolllike receptor 9(TLR9)/NF-κB pathway.Conclusion The integration of network pharmacology and animal experiments suggests that FSE alleviates renal inflammation and damage in IgAN through the TLR9/NF-κB signaling pathway.
文摘Traditional Medicine Research published an article entitled Investigation on the mechanism of Qi-Hong-Tang-Zu ointment in thetreatmentofdiabetic wound by network pharmacology and experimental validation on 24 January 2025.The author confirmed this article’s proof on 23 January 2025 without any questions.But on 06 July 2025,the editorial team found,there was an error in Figure 8.Specifically,Figure 8E,Figure 8F and Figure 8G were mistakenly duplicated.The authors acknowledge that the original version of Figure 8 was incorrect and have provided the revised,correct version in this corrigendum.The authors would like to assert that there is no change in the body text of the article.
基金supported in part by the Nation Natural Science Foundation of China under Grant No.52175099China Postdoctoral Science Foundation under Grant No.2020M671494Jiangsu Planned Projects for Postdoctoral Research Funds under Grant No.2020Z179。
文摘A tracking stability control problem for the vertical electric stabilization system of moving tank based on adaptive robust servo control is addressed.This paper mainly focuses on two types of possibly fast timevarying but bounded uncertainty within the vertical electric stabilization system:model parameter uncertainty and uncertain nonlinearity.First,the vertical electric stabilization system is constructed as an uncertain nonlinear dynamic system that can reflect the practical mechanics transfer process of the system.Second,the dynamical equation in the form of state space is established by designing the angular tracking error.Third,the comprehensive parameter of system uncertainty is designed to estimate the most conservative effects of uncertainty.Finally,an adaptive robust servo control which can effectively handle the combined effects of complex nonlinearity and uncertainty is proposed.The feasibility of the proposed control strategy under the practical physical condition is validated through the tests on the experimental platform.This paper pioneers the introduction of the internal nonlinearity and uncertainty of the vertical electric stabilization system into the settlement of the tracking stability control problem,and validates the advanced servo control strategy through experiment for the first time.
文摘To accelerate the practicality of electromagnetic railguns,it is necessary to use a combination of threedimensional numerical simulation and experiments to study the mechanism of bore damage.In this paper,a three-dimensional numerical model of the augmented railgun with four parallel unconventional rails is introduced to simulate the internal ballistic process and realize the multi-physics field coupling calculation of the rail gun,and a test experiment of a medium-caliber electromagnetic launcher powered by pulse formation network(PFN)is carried out.Various test methods such as spectrometer,fiber grating and high-speed camera are used to test several parameters such as muzzle initial velocity,transient magnetic field strength and stress-strain of rail.Combining the simulation results and experimental data,the damage condition of the contact surface is analyzed.
基金Supported by National Natural Science Foundation of China,No.81573695,No.81860894,and No.81674096Ningxia Key Research and Development Plan Project,No.2021BEG03106.
文摘BACKGROUND Diabetic kidney disease(DKD)is one of the serious complications of diabetes mellitus,and the existing treatments cannot meet the needs of today's patients.Traditional Chinese medicine has been validated for its efficacy in DKD after many years of clinical application.However,the specific mechanism by which it works is still unclear.Elucidating the molecular mechanism of the Nardostachyos Radix et Rhizoma-rhubarb drug pair(NRDP)for the treatment of DKD will provide a new way of thinking for the research and development of new drugs.AIM To investigate the mechanism of the NRDP in DKD by network pharmacology combined with molecular docking,and then verify the initial findings by in vitro experiments.METHODS The Traditional Chinese Medicine Systems Pharmacology(TCMSP)database was used to screen active ingredient targets of NRDP.Targets for DKD were obtained based on the Genecards,OMIM,and TTD databases.The VENNY 2.1 database was used to obtain DKD and NRDP intersection targets and their Venn diagram,and Cytoscape 3.9.0 was used to build a"drug-component-target-disease"network.The String database was used to construct protein interaction networks.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis and Gene Ontology analysis were performed based on the DAVID database.After selecting the targets and the active ingredients,Autodock software was used to perform molecular docking.In experimental validation using renal tubular epithelial cells(TCMK-1),we used the Cell Counting Kit-8 assay to detect the effect of NRDP on cell viability,with glucose solution used to mimic a hyperglycemic environment.Flow cytometry was used to detect the cell cycle progression and apoptosis.Western blot was used to detect the protein expression of STAT3,p-STAT3,BAX,BCL-2,Caspase9,and Caspase3.RESULTS A total of 10 active ingredients and 85 targets with 111 disease-related signaling pathways were obtained for NRDP.Enrichment analysis of KEGG pathways was performed to determine advanced glycation end products(AGEs)-receptor for AGEs(RAGE)signaling as the core pathway.Molecular docking showed good binding between each active ingredient and its core targets.In vitro experiments showed that NRDP inhibited the viability of TCMK-1 cells,blocked cell cycle progression in the G0/G1 phase,and reduced apoptosis in a concentrationdependent manner.Based on the results of Western blot analysis,NRDP differentially downregulated p-STAT3,BAX,Caspase3,and Caspase9 protein levels(P<0.01 or P<0.05).In addition,BAX/BCL-2 and p-STAT3/STAT3 ratios were reduced,while BCL-2 and STAT3 protein expression was upregulated(P<0.01).CONCLUSION NRDP may upregulate BCL-2 and STAT3 protein expression,and downregulate BAX,Caspase3,and Caspase9 protein expression,thus activating the AGE-RAGE signaling pathway,inhibiting the vitality of TCMK-1 cells,reducing their apoptosis.and arresting them in the G0/G1 phase to protect them from damage by high glucose.
文摘Gastric cancer(GC)remains a formidable global health concern with significant morbidity and mortality rates,despite the fact that numerous advances have been made to improve conventional therapies.Xiaojianzhong decoction(XJZ),a traditional Chinese medicine,has garnered academic attention as a multicomponent,multitarget approach to managing GC.The present editorial explores the potential of XJZ in the treatment of GC through a comprehensive analysis of network pharmacology and experimental validation.Network pharmacology was used to identify key molecular targets of XJZ,including interleukin 6,prostaglandin-endoperoxide synthase 2,and matrix metalloproteinase 9,and in vitro experiments were used to confirm the efficacy of XJZ in inhibiting cell proliferation,inducing apoptosis,and modulating gene expression associated with GC progression.This editorial highlights XJZ as a promising therapeutic strategy for GC and indicates a need for further clinical exploration and validation of its efficacy.
基金supported by is supported by the Shanghai Municipal Science and Technology Major Projectthe support from Shanghai Super Postdoctoral Incentive Program
文摘Focusing on revealing the origin of high ammonia yield rate on Cu via nitrate reduction(NO3RR),we herein applied constant potential method via grand-canonical density functional theory(GC-DFT)with implicit continuum solvation model to predict the reaction energetics of NO3RR on pure copper surface in alkaline media.The potential-dependent mechanism on the most prevailing Cu(111)and the minor(100)and(110)facets were established,in consideration of NO_(2)_(−),NO,NH_(3),NH_(2)OH,N_(2),and N_(2)O as the main products.The computational results show that the major Cu(111)is the ideal surface to produce ammonia with the highest onset potential at 0.06 V(until−0.37 V)and the highest optimal potential at−0.31 V for ammonia production without kinetic obstacles in activation energies at critical steps.For other minor facets,the secondary Cu(100)shows activity to ammonia from−0.03 to−0.54 V with the ideal potential at−0.50 V,which requires larger overpotential to overcome kinetic activation energy barriers.The least Cu(110)possesses the longest potential range for ammonia yield from−0.27 to−1.12 V due to the higher adsorption coverage of nitrate,but also with higher tendency to generate di-nitrogen species.Experimental evaluations on commercial Cu/C electrocatalyst validated the accuracy of our proposed mechanism.The most influential(111)surface with highest percentage in electrocatalyst determined the trend of ammonia production.In specific,the onset potential of ammonia production at 0.1 V and emergence of yield rate peak at−0.3 V in experiments precisely located in the predicted potentials on Cu(111).Four critical factors for the high ammonia yield and selectivity on Cu surface via NO3RR are summarized,including high NO3RR activity towards ammonia on the dominant Cu(111)facet,more possibilities to produce ammonia along different pathways on each facet,excellent ability for HER inhibition and suitable surface size to suppress di-nitrogen species formation at high nitrate coverage.Overall,our work provides comprehensive potential-dependent insights into the reaction details of NO3RR to ammonia,which can serve as references for the future development of NO3RR electrocatalysts,achieving higher activity and selectivity by maximizing these characteristics of copper-based materials.
基金supported by the Fundamental Research Funds for the Central Universities(No.30922010719).
文摘Underwater jet propulsion bio-inspired robots have typically been designed based on soft-bodied organisms, exhibiting relatively limited forms of locomotion. Scallop, a bivalve organism capable of jet propulsion, holds significant importance in the study of underwater motion mechanisms. In this study, we present theoretical fluid mechanics analysis and modeling of the three distinct motion stages of scallops, providing parameterized descriptions of scallop locomotion mechanisms. Accordingly, three-stage adaptive motion control for the scallop robot and model-based robot configuration optimization design were achieved. An experimental platform and a robot prototype were built to validate the accuracy of the motion model and the effectiveness of the control strategy. Additionally, based on the models, future optimization directions for the robot are proposed.
文摘3D printing technology can realize the rapid fabrication of complicated structures with short production chain,which just meet the requirements for space manufacturing in the future.This Special Issue features the cutting-edge 3D printing technologies considering the space environment,focusing on the experimental validation and simulation on the 3D printing process and structural technologies,including whole process chain from raw materials,structural design,process,equipment,as well as functional verification.
基金National Natural Science Foundation of China(Grant Nos.52408314,52278292)Chongqing Outstanding Youth Science Foundation(Grant No.CSTB2023NSCQ-JQX0029)+1 种基金Science and Technology Project of Sichuan Provincial Transportation Department(Grant No.2023-ZL-03)Science and Technology Project of Guizhou Provincial Transportation Department(Grant No.2024-122-018).
文摘Lost acceleration response reconstruction is crucial for assessing structural conditions in structural health monitoring(SHM).However,traditional methods struggle to address the reconstruction of acceleration responses with complex features,resulting in a lower reconstruction accuracy.This paper addresses this challenge by leveraging the advanced feature extraction and learning capabilities of fully convolutional networks(FCN)to achieve precise reconstruction of acceleration responses.In the designed network architecture,the incorporation of skip connections preserves low-level details of the network,greatly facilitating the flow of information and improving training efficiency and accuracy.Dropout techniques are employed to reduce computational load and enhance feature extraction.The proposed FCN model automatically extracts high-level features from the input data and establishes a nonlinearmapping relationship between the input and output responses.Finally,the accuracy of the FCN for structural response reconstructionwas evaluated using acceleration data from an experimental arch rib and comparedwith several traditional methods.Additionally,this approach was applied to reconstruct actual acceleration responses measured by an SHM system on a long-span bridge.Through parameter analysis,the feasibility and accuracy of aspects such as available response positions,the number of available channels,and multi-channel response reconstruction were explored.The results indicate that this method exhibits high-precision response reconstruction capability in both time and frequency domains.,with performance surpassing that of other networks,confirming its effectiveness in reconstructing responses under various sensor data loss scenarios.
基金supported by the Guangdong High Level Innovation Research Institute(Grant No.2021B0909050006)the National Grand Instrument Project(Grant No.2019YFF01014402)+1 种基金the National Natural Science Foundation of China(Grant No.12205008)support from the National Science Fund for Distinguished Young Scholars(Grant No.12225501)。
文摘Experimental validation of laser intensity is particularly important for the study of fundamental physics at extremely high intensities.However,reliable diagnosis of the focal spot and peak intensity faces huge challenges.In this work,we demonstrate for the firs time that the coherent radiation farfiel patterns from laser–foil interactions can serve as an in situ,real-time,and easy-to-implement diagnostic for an ultraintense laser focus.The laser-driven electron sheets,curved by the spatially varying laser fiel and leaving the targets at nearly the speed of light,produce doughnut-shaped patterns depending on the shapes of the focal spot and the absolute laser intensities.Assisted by particle-in-cell simulations,we can achieve measurements of the intensity and the focal spot,and provide immediate feedback to optimize the focal spots for extremely high intensity.
基金the project SILVARSTAR funded from the Shift2Rail Joint Undertaking under the European Union’s Horizon 2020 Research and Innovation Programme under Grant Agreement 101015442。
文摘Within the SILVARSTAR project,a user-friendly frequency-based hybrid prediction tool has been developed to assess the environmental impact of railway-induced vibration.This tool is integrated in existing noise mapping software.Following modern vibration standards and guidelines,the vibration velocity level in a building in each frequency band is expressed as the sum of a force density(source term),line source transfer mobility(propagation term)and building correction factor(receiver term).A hybrid approach is used that allows for a combination of experimental data and numerical predictions,providing increased flexibility and applicability.The train and track properties can be selected from a database or entered as numerical values.The user can select soil impedance and transfer functions from a database,pre-computed for a wide range of parameters with state-of-the-art models.An experimental database of force densities,transfer functions,free field vibration and input parameters is also provided.The building response is estimated by means of building correction factors.Assumptions within the modelling approach are made to reduce computation time but these can influence prediction accuracy;this is quantified for the case of a nominal intercity train running at different speeds on a ballasted track supported by homogeneous soil of varying stiffness.The paper focuses on the influence of these parameters on the compliance of the track–soil system and the free field response.We also demonstrate the use and discuss the validation of the vibration prediction tool for the case of a high-speed train running on a ballasted track in Lincent(Belgium).
基金Acknowledgements This research is sponsored by the Major State Basic Research Development Program of China （＂973＂ Program） （2013CB036203）, the 111 Project （B13002）, and the National Natural Science Foundation of China （U1434205, U1434210, 51338001 ）.
文摘The train-bridge dynamic interaction problem began with the development of railway technology, and requires an evaluation method for bridge design in order to ensure the safety and stability of the bridge and the running train. This problem is studied using theoretical analysis, numerical simulation, and experimental study. In the train-bridge dynamic interaction system proposed in this paper, the train vehicle model is established by the rigid-body dynamics method, the bridge model is established by the finite element method, and the wheel/rail vertical and lateral interaction are simulated by the corresponding assumption and the Kalker linear creep theory, respectively. Track irregularity, structure deformation, wind load, collision load, structural damage, foundation scouring, and earthquake action are regarded as the excitation for the system. The train-bridge dynamic interaction system is solved by inter-history iteration. A case study of the dynamic response of a CRH380BL high-speed train running through a standard-design bridge in China is discussed. The dynamic responses of the vehicle and of the bridge subsystems are obtained for speeds ranging from 200 km-b-1 to 400 km.h-1, and the vibration mechanism are analyzed.
基金supported by National Basic Research Program of China(No.2007CB613700)National Sci & Tech Support Program (No.2007BAG06B04)+2 种基金National Natural Science Foundation of China (No.50725413)Plan for the 11th National Five-Year Plan (No.2006BAE04B03)Researches on Mathematical Models of Solidification Segregation in Continuous Casting and Simulation Technologies(No.CST,2007bb4413)
文摘To reduce the surface cracks of extrusion rod for AZ31 magnesium caused by nonhomogeneous metal flow in extrusion process, 3D computer finite element （FE） simulations of extruding a wrought magnesium alloy AZ31 into rods have been performed and the results have been verified in extrusion experiments under identical conditions. The tendency to generate the dead zone is decreased by employing the die angle 60° at the cone-shaped die comparing with the die angle 180°. The surface additional tensile stresses of the rod at the die exit are decreased greatly so that the surface cracks caused are avoided by using the die angle 60°. The extrusion die with die angle 180° would increase the higher temperature rise and possibility of crack formation on the rod surface that caused by die angle 60° and temperature rise decrease tensile strength of the AZ31 rod. The experimental results show that die angle 180° could cause continuous cracks on the surface of the extruded rod. The extrusion force required is reduced approximately 15 ton by employing the die angle 60°. Theoretical results obtained by the DeformTM-3D simulation agreed well with the experiments. The obtained results provide the fundamental and also practical guidelines for the design and correction of dies to produce magnesium rod with good surface quality.
基金EU Fifth Framework Program: Environment, Energy and Sustainable Development Research and Technological Development Activity of Generic Nature: The Fight Against Natural and Technological Hazards, Research Project QUAKER Under Contract No. EVG1–CT–2002–00064
文摘To gain insight into the inelastic behavior of piles, the response of a vertical pile embedded in dry sand and subjected to cyclic lateral loading was studied experimentally in centrifuge tests conducted in Laboratoire Central des Ponts et Chaussees. Three types of cyclic loading were applied, two asymmetric and one symmetric with respect to the unloaded pile. An approximately square-root variation of soil stiffness with depth was obtained from indirect in-flight density measurements, laboratory tests on reconstituted samples, and well-established empirical correlations. The tests were simulated using a cyclic nonlinear Winkler spring model, which describes the full range of inelastic phenomena, including separation and re-attachment of the pile from and to the soil. The model consists of three mathematical expressions capable of reproducing a wide variety of monotonic and cyclic experimentalp-y curves. The physical meaning of key model parameters is graphically explained and related to soil behavior. Comparisons with the centrifuge test results demonstrate the general validity of the model and its ability to capture several features of pile-soil interaction, including： soil plastification at an early stage of loading, ＂pinching＂ behavior due to the formation of a relaxation zone around the upper part of the pile, and stiffness and strength changes due to cyclic loading. A comparison of the p-y curves derived from the test results and the proposed model, as well as those from the classical curves of Reese et al. （1974） for sand, is also presented.
基金supported by National Natural Science Foundation of China(Grant No.51275039)
文摘The current design of hydro-viscous clutch（HVC） in tracked vehicle fan transmission mainly focuses on high-speed and high power. However, the fluid torque under the influence of fluid temperature can not be predicted accurately by conventional mathematical model or experimental research. In order to validate the fluid torque of HVC by taking the viscosity-temperature characteristic of fluid into account, the test rig is designed. The outlet oil temperature is measured and fitted with different rotation speed, oil film thickness, oil flow rate, and inlet oil temperature. Meanwhile, the film torque can be obtained. Based on Navier-Stokes equations and the continuity equation, the mathematical model of fluid torque is proposed in cylindrical coordinate. Iterative method is employed to solve the equations. The radial and tangential speed distribution, radial pressure distribution and theoretical flow rate are determined and analyzed. The models of equivalent radius and fluid torque of friction pairs are introduced. The experimental and theoretical results indicate that tangential speed distribution is mainly determined by the relative rotating speed between the friction plate and the separator disc. However, the radial speed distribution and pressure distribution are dominated by pressure difference at the lower rotating speed. The oil film fills the clearance and the film torque increases with increasing rotating speed. However, when the speed reaches a certain value, the centrifugal force will play an important role on the fluid distribution. The pressure is negative at the outer radius when inlet flow rate is less than theoretical flow, so the film starts to shrink which decreases the film torque sharply. The theoretical fluid torque has good agreement with the experimental data. This research proposes a new fluid torque mathematical model which may predict the film torque under the influence of temperature more accurately.
文摘With increasing amount of unconventional natural gas,the production of ethane,propane and other low alkanes continues to increase.In our previous works,a partially decoupled process(PDP) was proposed for conversion of ethane based on numerical simulations,which showed higher acetylene and ethylene selectivities than the original partial oxidation process.In the current work,the PDP of ethane for producing acetylene and ethylene was studied experimentally to verify the PDP concept.In the PDP of ethane,coke-oven gas or other cheap gas combusts with stoichiometric oxygen as heat carrier,and ethane is mixed with the heat carrier and undergoes pyrolysis at high temperatures.The jet-in-cross-flow(JICF) reactor was designed and manufactured to realize the PDP.A positioning device of 0.1 mm accuracy and a mass spectrometer were used to measure the spatial profiles of the species concentrations.The maximum combined yield(52.7%) of acetylene and ethylene was obtained even at the condition of heat loss,confirming that the PDP of ethane was advantageous over the partial oxidation process and at least comparable to the steam cracking process.
基金Project (No. 2006AA11A127) supported by the Hi-Tech (863) Research and Development Program of China
文摘This paper aims to present the configuration design approach and the energy management strategy （EMS） of a series-parallel hybrid electric transit bus （SPHEB） jointly developed by Shanghai Automotive Industry Co. Ltd. （SAIC） and Shanghai Jiao Tong University （SJTU）, China. A major feature of this SPHEB is that a novel manual transmission is designed to switch the powertrain configuration between series and parallel types. To reduce the fuel consumption as well as sustain the battery state of charge, an EMS including seven energy flow modes is designed and applied to this SPHEB. Governed by this EMS, the engine is maintained to operate in high efficiency regions. The experimental test carded on the transit bus city driving cycle is described and analyzed. The experimental results demonstrate the technical feasibility and fuel economy of this approach.
基金Supported by the National Natural Science Foundation of China (30570990, 30471059, 31171578)the "863" project (2008AA10Z153)+2 种基金the Key Research Plan of Heilongjiang Province (GA06B103-3)the Innovation Research Group of NEAU (CXT004)the Research Fund for the Doctoral Program of Higher Education of China (20102325120002)
文摘MicroRNAs （miRNAs） are a group of regulatory RNAs that regulate gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs （mRNAs）. Regulation is accomplished when the 22-25 nucleotide miRNAs bind to complementary sequences in the 3＇-untranslated regions （UTR）. One barrier to miRNA research is to find target genes. Although computational target predictions have shed light on important aspects of microRNA target recognition, questions remain concerning the rates of false positives. In addition, we do not completely understand how microRNAs can recognize and regulate their targets. As such, experimental positive predictions and allow for an unbiased stu ap dy proaches are required, which can reflect in vivo processes, eliminating false of microRNA target recognition. In this review, we summarized experimental approaches that have been described for the identification and validation of mRNA targets associated with specific miRNAs.
