Cephalosporins play an indispensable role against bacterial infections.Deacetyloxycephalosporin C synthase(DAOCS),also called expandase,is a key enzyme in cephalosporin biosynthesis that epoxides penicillin to form th...Cephalosporins play an indispensable role against bacterial infections.Deacetyloxycephalosporin C synthase(DAOCS),also called expandase,is a key enzyme in cephalosporin biosynthesis that epoxides penicillin to form the hexavalent thiazide ring of cephalosporin.DAOCS in fungus Acremonium chrysogenum was identified as a bifunctional enzyme with both ring expansion and hydroxylation,whereas two separate enzymes in bacteria catalyze these two reactions.In this review,we briefly summarize its source and function,improvement of the conversion rate of penicillin to deacetyloxycephalosporin C through enzyme modification,crystallography features,the prediction of the active site,and application perspective.展开更多
基金This work was supported by the Key Project of Natural Science Foundation of Tianjin Municipal Science and Technology Bureau of China(20JCZDJC00140)National Basic Research Program(973 Program)of China(2013CB734004)+1 种基金National Natural Science Foundation of China(31370075,31471725,31902193,61603273)Key Technology R&D Project of Shandong Province of China(Fostering Talent Project)(2016GRC3201).
文摘Cephalosporins play an indispensable role against bacterial infections.Deacetyloxycephalosporin C synthase(DAOCS),also called expandase,is a key enzyme in cephalosporin biosynthesis that epoxides penicillin to form the hexavalent thiazide ring of cephalosporin.DAOCS in fungus Acremonium chrysogenum was identified as a bifunctional enzyme with both ring expansion and hydroxylation,whereas two separate enzymes in bacteria catalyze these two reactions.In this review,we briefly summarize its source and function,improvement of the conversion rate of penicillin to deacetyloxycephalosporin C through enzyme modification,crystallography features,the prediction of the active site,and application perspective.
