Exosomes(EXOs)play an important role in the progression of breast cancer.EXOs,with a diameter of approximately 100 nm,have a simple structure but diverse functions,and can affect the development of breast cancer throu...Exosomes(EXOs)play an important role in the progression of breast cancer.EXOs,with a diameter of approximately 100 nm,have a simple structure but diverse functions,and can affect the development of breast cancer through signal transduction and molecular transfer,etc.Angiogenesis provides nutrients for the growth and metastasis of breast cancer and is a crucial part of tumor progression.The mechanism of tumor angiogenesis is complex.The VEGF/VEGFR pathway promotes angiogenesis by regulating the activities of ECs.Hypoxia,a common feature in the tumor microenvironment,as a key regulator,can affect angiogenesis in multiple aspects such as the transfer of miRNAs in EXOs,protein transport,extracellular matrix regulation,and metabolic adaptation.The Notch pathway has a bidirectional regulatory role in breast cancer angiogenesis,and different molecules can promote or inhibit angiogenesis.EXOs secreted by breast cancer cells are rich in angiogenic factors.Components such as proteins and nucleic acids in EXOs can affect the functions and behaviors of vascular ECs,thereby influencing breast cancer angiogenesis.Research on the mechanisms of EXOs in breast cancer angiogenesis is of great significance for tumor treatment.EXOs are expected to become biomarkers for breast cancer diagnosis/prognosis.This research provides potential targets for in-depth understanding of the biological characteristics of breast cancer and the development of new treatment strategies.展开更多
This research is examining the degree of implementation of the attributes of exponential organization by Jordanian services companies listed on Amman Stock Exchange(ASE);around 68 companies will be examined,through th...This research is examining the degree of implementation of the attributes of exponential organization by Jordanian services companies listed on Amman Stock Exchange(ASE);around 68 companies will be examined,through their annual changes of their increase on their budget over the last 11 years.The change and increase of their annual budgets or the total value of these companies will be a great indicator for exponential trend and behaviour of these companies.The study is an attempt to examine the degree that companies listed in ASE follow internal characteristics of EXOs developed by Ismail Salim and do the capital structure of EXOs differed from the capital structure listed in ASE.展开更多
Objective:To use the gene chip of pseudomonas aeruginosa as a research sample and to explore it at an omics level,aiming at elucidating the co-expression network characteristics of the virulence genes exoS and exoU of...Objective:To use the gene chip of pseudomonas aeruginosa as a research sample and to explore it at an omics level,aiming at elucidating the co-expression network characteristics of the virulence genes exoS and exoU of pseudomonas aeruginosa in the lower respiratory tract from the perspective of molecular biology and identifying its key regulatory genes.Methods:From March 2016 to May 2018,312 patients infected with pseudomonas aeruginosa in the lower respiratory tract who were admitted to Department of Respiratory Medicine of Baogang Hospital and given follow-up treatments in the hospital were selected as subjects by use of cluster sampling.Alveolar lavage fluid and sputum collected from those patients were used as biological specimens.The genes of pseudomonas aeruginosa were detected with the help of oligonucleotide probes to make a pre-processing of chip data.A total of 8 common antibiotics(ceftazidime,gentamicin,piperacillin,amikacin,ciprofloxacin,levofloxacin,doripenem and ticarcillin)against Gram-negative bacteria were selected to determine the drug resistance of biological specimens.MCODE algorithm was used to construct a co-expression network model of the drug-resistance genes focused on exoS/exoU.Results:The expression level of exoS/exoU in the drug-resistance group was significantly higher than that in the non-resistance group(p<0.05).The top 5 differentially expressed genes in the alveolar lavage fluid specimens from the drug-resistance group were RAC1,ITGB1,ITGB5,CRK and IGF1R in the order from high to low.In the sputum specimens,the top 5 differentially expressed genes were RAC1,CRK,IGF1R,ITGB1 and ITGB5.In the alveolar lavage fluid specimens,only RAC1 had a positive correlation with the expression of exoS and exoU(p<0.05).In the sputum specimens,RAC1,ITGB1,ITGB5,CRK and IGF1R were positively correlated with the expression of exoS and exoU(p<0.05).The genes included in the co-expression network contained exoS,exoU,RAC1,ITGB1,ITGB5,CRK,CAMK2D,RHOA,FLNA,IGF1R,TGFBR2 and FOS.Among them,RAC1 had a highest score in the aspect of regulatory ability(72.00)and the largest number of regulatory genes(6);followed by ITGB1,ITGB5 and CRK genes.Conclusions:The high expression of exoS and exoU in the sputum specimens suggests that pseudomonas aeruginosa has a higher probability to get resistant to antibiotics;RAC1,ITGB1,ITGB5 and CRK genes may be the key genes that can regulate the expression of exoS and exoU.展开更多
文摘Exosomes(EXOs)play an important role in the progression of breast cancer.EXOs,with a diameter of approximately 100 nm,have a simple structure but diverse functions,and can affect the development of breast cancer through signal transduction and molecular transfer,etc.Angiogenesis provides nutrients for the growth and metastasis of breast cancer and is a crucial part of tumor progression.The mechanism of tumor angiogenesis is complex.The VEGF/VEGFR pathway promotes angiogenesis by regulating the activities of ECs.Hypoxia,a common feature in the tumor microenvironment,as a key regulator,can affect angiogenesis in multiple aspects such as the transfer of miRNAs in EXOs,protein transport,extracellular matrix regulation,and metabolic adaptation.The Notch pathway has a bidirectional regulatory role in breast cancer angiogenesis,and different molecules can promote or inhibit angiogenesis.EXOs secreted by breast cancer cells are rich in angiogenic factors.Components such as proteins and nucleic acids in EXOs can affect the functions and behaviors of vascular ECs,thereby influencing breast cancer angiogenesis.Research on the mechanisms of EXOs in breast cancer angiogenesis is of great significance for tumor treatment.EXOs are expected to become biomarkers for breast cancer diagnosis/prognosis.This research provides potential targets for in-depth understanding of the biological characteristics of breast cancer and the development of new treatment strategies.
文摘This research is examining the degree of implementation of the attributes of exponential organization by Jordanian services companies listed on Amman Stock Exchange(ASE);around 68 companies will be examined,through their annual changes of their increase on their budget over the last 11 years.The change and increase of their annual budgets or the total value of these companies will be a great indicator for exponential trend and behaviour of these companies.The study is an attempt to examine the degree that companies listed in ASE follow internal characteristics of EXOs developed by Ismail Salim and do the capital structure of EXOs differed from the capital structure listed in ASE.
文摘Objective:To use the gene chip of pseudomonas aeruginosa as a research sample and to explore it at an omics level,aiming at elucidating the co-expression network characteristics of the virulence genes exoS and exoU of pseudomonas aeruginosa in the lower respiratory tract from the perspective of molecular biology and identifying its key regulatory genes.Methods:From March 2016 to May 2018,312 patients infected with pseudomonas aeruginosa in the lower respiratory tract who were admitted to Department of Respiratory Medicine of Baogang Hospital and given follow-up treatments in the hospital were selected as subjects by use of cluster sampling.Alveolar lavage fluid and sputum collected from those patients were used as biological specimens.The genes of pseudomonas aeruginosa were detected with the help of oligonucleotide probes to make a pre-processing of chip data.A total of 8 common antibiotics(ceftazidime,gentamicin,piperacillin,amikacin,ciprofloxacin,levofloxacin,doripenem and ticarcillin)against Gram-negative bacteria were selected to determine the drug resistance of biological specimens.MCODE algorithm was used to construct a co-expression network model of the drug-resistance genes focused on exoS/exoU.Results:The expression level of exoS/exoU in the drug-resistance group was significantly higher than that in the non-resistance group(p<0.05).The top 5 differentially expressed genes in the alveolar lavage fluid specimens from the drug-resistance group were RAC1,ITGB1,ITGB5,CRK and IGF1R in the order from high to low.In the sputum specimens,the top 5 differentially expressed genes were RAC1,CRK,IGF1R,ITGB1 and ITGB5.In the alveolar lavage fluid specimens,only RAC1 had a positive correlation with the expression of exoS and exoU(p<0.05).In the sputum specimens,RAC1,ITGB1,ITGB5,CRK and IGF1R were positively correlated with the expression of exoS and exoU(p<0.05).The genes included in the co-expression network contained exoS,exoU,RAC1,ITGB1,ITGB5,CRK,CAMK2D,RHOA,FLNA,IGF1R,TGFBR2 and FOS.Among them,RAC1 had a highest score in the aspect of regulatory ability(72.00)and the largest number of regulatory genes(6);followed by ITGB1,ITGB5 and CRK genes.Conclusions:The high expression of exoS and exoU in the sputum specimens suggests that pseudomonas aeruginosa has a higher probability to get resistant to antibiotics;RAC1,ITGB1,ITGB5 and CRK genes may be the key genes that can regulate the expression of exoS and exoU.
