Background Nitrogen-Use-Efficiency(NUE)in lactating dairy cows,defined as milk nitrogen(N)output as a proportion of N consumed,is low,with the majority of excess N excreted in manure.Excreted N can be lost to the envi...Background Nitrogen-Use-Efficiency(NUE)in lactating dairy cows,defined as milk nitrogen(N)output as a proportion of N consumed,is low,with the majority of excess N excreted in manure.Excreted N can be lost to the environment as ammonia gas leading to environmental acidification and nutrient enrichment of sensitive habitats,and to watercourses contributing to aquatic eutrophication.While there is much evidence that NUE can be improved by reducing the crude protein(CP)content of dairy cow diets,the long-term impacts of feeding lower protein diets on cow performance and the rumen microbiome are less well understood.This study examined the effects of reducing the CP contents of dairy cow diets on cow performance,NUE,the relationship between NUE and residual feed intake(RFI),and the rumen microbiome.Results Dietary CP content did not affect feed intake,milk yield or milk composition(P>0.05),except for milk urea N(MUN),which increased with increasing diet CP content(P<0.05).The mean NUE was 34%,34%and 31%for the LCP(low-protein,15%),MCP(medium-protein,16%),and HCP(high-protein,17%)diets,respectively.RFI was negatively correlated with NUE(r=−0.57,P<0.001).The rumen ammonia-N concentrations increased with increasing dietary CP;however,the ruminal pH and volatile fatty acid(VFA)content of the rumen fluid remained constant.Predicted urinary N excretion was greater in the HCP and MCP diets than in the LCP diet.Reducing dietary CP content in dairy cow diets did not affect microbial composition,diversity and functional profiles.The family Bacteroidaceae was more abundant in HE(high-efficiency)cows,whereas the Methanobacteriaceae and the genus Methanobrevibacter were more abundant in LE(low-efficiency)cows.Additionally,propanoate metabolism,cysteine and methionine metabolism and amino acid biosynthesis pathways were more abundant in HE cows,whilst the methane(CH4)metabolism pathway was upregulated in LE cows.Conclusions The results demonstrate that diet CP can be reduced with no loss in cow performance,but with an associated reduction in N excretion.The abundance of microbial populations differed between low and high efficiency cows,which may contribute to the differences in efficiency observed.展开更多
Objective: To analyze the possible mechanism of Pueraria isoflavones inhibiting XOD and GLUT9 to reduce uric acid production and promote uric acid excretion. Methods: August 2021-April 2022, a total of forty SPF male ...Objective: To analyze the possible mechanism of Pueraria isoflavones inhibiting XOD and GLUT9 to reduce uric acid production and promote uric acid excretion. Methods: August 2021-April 2022, a total of forty SPF male Kunming mice were divided into the healthy group (carboxymethylcellulose sodium at a dose of 250 mg/kg), the model group (HUA mice were given carboxymethylcellulose sodium at a dose of 250 mg/kg), the low group (HUA mice were given pueraria isoflavone at a dose of 125 mg/kg), HUA mice were given pueraria isoflavones at a dose of 250 mg/kg once d frequency)and the high group (HUA mice were given pueraria isoflavones at a dose of 500 mg/kg once d frequency) dosage groups, with 8 mice in each group. The contents of uric acid (SUA), urea nitrogen (BUN) and creatinine (SCr) in serum and urine of each group were compared before and after intervention (30 d). Statistical differences of xanthine oxidase (XOD) and human glucose transporter 9(GLUT9), cy- clooxygenase- 2(COX-2), tumor necrosis factor (TNF-α) and interleukin-1 (IL-1β) contents in renal tissues of each group after intervention (30 d) were compared. Results: After intervention, kidney inflammatory factors (COX-2, TNF-α and IL-1β) in the model group were compared. Blood and urine indexes (SUA, BUN, SCr);The contents of XOD and GLUT9 were higher than those of healthy group(P<0.05). Renal inflammatory cytokines (COX-2, TNF-α and IL-1β) in low, medium and high dose groups;Blood and urine indexes (SUA, BUN, SCr);The contents of XOD and GLUT9 were lower than those of model group, and there were low > medium > high dose groups, the comparison between the two groups had statistical significance(P< 0.05). After intervention, the contents of 3 indicators in blood or urine(COX-2, TNF-α and IL-1β) all decreased compared with before intervention, and the differences in intra-group comparison were statistically significant (P<0.05). Conclusion: Pueraria isoflavones can treat HUA mice by inhibiting the expression of XOD and GLUT9, and then play a role in reducing uric acid pro- duction and promoting uric acid excretion, as well as alleviating the degree of disease inflammation.展开更多
Biofouling is a particular problem in the pearl oyster culture. It may reduce the growth and survival rates of the cultured species. Foulers are often themselves filter feeders, and are therefore potential competitors...Biofouling is a particular problem in the pearl oyster culture. It may reduce the growth and survival rates of the cultured species. Foulers are often themselves filter feeders, and are therefore potential competitors for food resource with the cultured species. Fouling organisms may also reduce the oxygen supply. A study was conducted to measure the impact of foulers on feeding oxygen consumption, and waste excretion. POM, ammonia, phosphate and oxygen concentration were measured in various treatments （cultured species, foulers）. This study showed that fouling organisms had significant effect on food uptake, oxygen consumption and waste excretion. The clearance rate, ammonia and phosphate release rate, oxygen consumption rate of the fouled pearl oyster were greater than those of the clean pearl oysters. Other foulers that settled on cages or buoys also contributed much to phytoplankton depletion, oxygen consumption and concentration increase of ammonia and phosphate in water. Therefore, this study showed us that foulers were important competitors in the pearl oyster cultivation of Daya Bay in November 2005.展开更多
AIMTo determine the accuracy of fractional excretion of sodium (FeNa) in the diagnosis of hepatorenal syndrome (HRS). METHODSEighty-eight liver transplantation candidates with renal dysfunction and/or proteinuria were...AIMTo determine the accuracy of fractional excretion of sodium (FeNa) in the diagnosis of hepatorenal syndrome (HRS). METHODSEighty-eight liver transplantation candidates with renal dysfunction and/or proteinuria were included in the study sample. The baseline characteristics of the patients were obtained. All the 88 patients underwent iothalamate glomerular filtration rate testing, 24-h urine collection for urinary sodium and protein excretions, random urine for sodium and creatinine testing, and percutaneous kidney biopsy. FeNa was calculated using the equation [(urine sodium × serum creatinine)/(serum sodium × urine creatinine)] × 100%. Diuretic use was recorded among the participants. Patients on renal replacement therapy were not included in the original sample. RESULTSSeventy-seven (87%) of the 88 patients had FeNa P = 0.4). FeNa P = 0.47). Calculated positive predictive value and negative predictive value for FeNa P = 0.41). CONCLUSIONFeNa 1%.展开更多
The present study aimed at studying the characteristics of tissue distribution and excretion of scopoletin,a coumarin compound,in Sprague-Dawley rats.Scopoletin was orally administered at a dose of 50 mg/kg,and the co...The present study aimed at studying the characteristics of tissue distribution and excretion of scopoletin,a coumarin compound,in Sprague-Dawley rats.Scopoletin was orally administered at a dose of 50 mg/kg,and the concentrations in heart,liver,spleen,lung,kidney,muscle,fat,brain,testis,uterus,stomach and small intestine were determined at 5,15,30,60,120,240 min post-dose,respectively.It was shown that scopoletin was widely distributed into various tissues and reached the maximal concentrations in most tissues at 15 min post-dose,and the levels in liver,kidney,stomach and small intestine were relatively higher.Furthermore,the excretions of scopoletin in bile,urine and feces were only 0.032%,3.752% and 0.784%,respectively,suggesting that scopoletin was mainly eliminated by metabolism rather than excretion as parent drug.展开更多
The massive COVID-19 vaccination campaign is the first time that mRNA vaccines have been used on a global scale.The mRNA vaccines correspond exactly to the definition of gene therapy of the American and European regul...The massive COVID-19 vaccination campaign is the first time that mRNA vaccines have been used on a global scale.The mRNA vaccines correspond exactly to the definition of gene therapy of the American and European regulatory agencies.The regulations require excretion studies of these drugs and their products(the translated proteins).These studies have not been done for mRNA vaccines(nor for adenovirus vaccines).There are numerous reports of symptoms and pathologies identical to the adverse effects of mRNA vaccines in unvaccinated persons in contact with freshly vaccinated persons.It is therefore important to review the state of knowledge on the possible excretion of vaccine nanoparticles as well as mRNA and its product,the spike protein.Vaccine mRNA-carrying lipid nanoparticles spread after injection throughout the body according to available animal studies and vaccine mRNA(naked or in nanoparticles or in natural exosomes)is found in the bloodstream as well as vaccine spike in free form or encapsulated in exosomes(shown in human studies).Lipid nanoparticles(or their natural equivalent,exosomes or extracellular vesicles(EVs))have been shown to be able to be excreted through body fluids(sweat,sputum,breast milk)and to pass the transplacental barrier.These EVs are also able to penetrate by inhalation and through the skin(healthy or injured)as well as orally through breast milk(and why not during sexual intercourse through semen,as this has not been studied).It is urgent to enforce the legislation on gene therapy that applies to mRNA vaccines and to carry out studies on this subject while the generalization of mRNA vaccines is being considered.展开更多
Background:This study was conducted to determine the effects of different dietary protein levels and amino acids supplementation patterns in low protein diets on the growth performance,carcass characteristics and nitr...Background:This study was conducted to determine the effects of different dietary protein levels and amino acids supplementation patterns in low protein diets on the growth performance,carcass characteristics and nitrogen excretion in growing-finishing pigs.Forty-two barrows(25.00±0.39 kg)were randomly assigned to 7 diets.Diet 1:the high crude protein diet with balanced for 10 essential amino acids(EAAs).Diet 2:the medium crude protein diet with 2%(approx)decreased protein level of Diet 1 and balanced 10 EAAs.Diet 3:the low crude protein diet with 4%decreased protein level of Diet 1 and balanced 10 EAAs.The protein levels of Diet 4,5,6 and 7 were the same as that of Diet 3.Diet 4 was only balanced for lysine(Lys),methionine(Met),threonine(Thr)and tryptophan(Trp);Diet 5 and 6 were further supplemented with extra isoleucine(Ile)or valine(Val),respectively;Diet 7 was further supplemented with extra Ile+Val.Results:Over the 112 days trial,the reduction of dietary protein by 2%or 4%with balanced10 EAAs significantly decreased nitrogen excretion(P<0.05),but had no effects on growth performance and carcass characteristics(P>0.05).In low protein diet,Val supplementation significantly increased body weight gain at 25–50 kg phase(P<0.05),while Ile supplementation at 75–100 kg phase and 100–125 kg phase significantly reduced the ratio of feed to gain(P<0.05).No effect of different dietary protein levels and amino acids supplementation patterns in low protein diets on carcass characteristics was observed(P>0.05).The total N excretion of pigs supplemented with only Lys,Met,Thr and Trp was numerically higher than that of pigs fed with extra Ile,or Val,or Ile+Val diets.Conclusion:In low protein diet,Val is more required than Ile in the early growing phage(25–50 kg),while Ile becomes more required in the late growing and finishing phage(75–125 kg).展开更多
Effects of temperatures and salinities on oxygen consumption and ammonia-N excretion rate of clam Meretrix meretrix were studied in laboratory from Oct. 2003 to Jan. 2004. Two schemes were designed in incremented temp...Effects of temperatures and salinities on oxygen consumption and ammonia-N excretion rate of clam Meretrix meretrix were studied in laboratory from Oct. 2003 to Jan. 2004. Two schemes were designed in incremented temperature at 10, 15, 20, 25℃ at 31.5 salinity and in incremented salinity at16.0, 21.0, 26.0, 31.5, 36.0, and 41.0 at 20℃, all for 8-10 days. From 10 to 25℃, both respiration and excretion rate were increased. One-way ANOVA analysis demonstrated significant difference （P〈0.01） in physiological parameters in this temperature range except between 15 and 20℃. The highest Q10 thermal coefficient value （12.27） was acquired between 10 and 15℃, and about 1 between 15 and 20℃, indicating M. meretrix could well acclimate to temperature changes in this range. Salinity also had significant effects on respiration and excretion rate （P〈0.05）. The highest values of respiration and excretion rate of M. meretrix were recorded at 16.0 salinity （20℃）. These two physiological parameters decreased as salinity increased until reached the minimum Q10 value at 31.5 （20℃）, then again, these parameters increased with increasing salinity from 31.5 to 41.0. M. meretrix can catabolize body protein to cope with osmotic pressure stress when environmental salinity is away from its optimal range. No significant difference was observed between 26.0 and 36.0 in salinity （P〉0.05）, suggesting that a best metabolic salinity range for this species is between 26.0 and 36.0.展开更多
Two experiments were conducted to investigate the effect of dietary supplementation with phytase transgenic corn （PTC） on growth performance,phosphorus （P） utilization and excretion in growing pigs.In Exp.1,180 pi...Two experiments were conducted to investigate the effect of dietary supplementation with phytase transgenic corn （PTC） on growth performance,phosphorus （P） utilization and excretion in growing pigs.In Exp.1,180 pigs （Large White × Landrace,BW=37.7 kg） were randomly allotted to 4 treatments with 5 replicates of 9 pigs each in order to evaluate the effect of PTC supplementation in low-P diets on growth performance.Four corn soybean meal-based diets consisted of a positive control （PC） diet,a diet containing 500 units （U） of exogenous phytase kg-1 （EP） on the basis of low-P （inorganic P reduced by 0.05% from PC diet） and the low-P＋500 （PTC1） or 750 （PTC2） phytase U of PTC kg-1.In Exp.2,20 barrows （Large White×Landrace,BW=31 kg,4 treatments with 5 replicates of 1 pig each） were randomly selected to evaluate the effect of PTC in low-P diets on serum parameters and nutrient utilization.Diets in Exp.2 were similar to those in Exp.1 except that the EP group was replaced by a low-P diet without exogenous phytase supplementation as a negative control （NC） group.The results from Exp.1 showed that the average daily gain （ADG） in the PTC2 group was significantly higher （P〈0.05） than that in the EP group over all periods.On the other hand,the feed：gain （F：G） ratio of the EP group was significantly higher （P〈0.05） than that of the PTC2 group during 1-21 and 1-42 d,respectively.There were no differences in average daily feed intake （ADFI） among all treatments （P〉0.05）.The results from Exp.2 showed that the concentration of serum Ca in the NC group was the highest （P〈0.05）,while the concentration of serum P in the PTC2 group was the highest （P〈0.05） among all treatments.There was a significant decrease （P〈0.05） in the P apparent digestibility of the NC group compared with the other groups,and that of PTC2 group was the best.Furthermore,fecal P excretion was reduced （P〈0.05） from 1.80 g d-1 in the PC group to 1.28 g d-1 in the PTC2 group.In conclusion,dietary supplementation with PTC could reduce the application of inorganic P,decrease fecal P excretion,and improve the growth performance of growing pigs.展开更多
Animal agriculture contributes to environmental pollutions through the surplus nitrogen（N）, phosphorus（P）, and trace minerals that animals excrete. Animal nutritionists have sought alternatives to formulate more e...Animal agriculture contributes to environmental pollutions through the surplus nitrogen（N）, phosphorus（P）, and trace minerals that animals excrete. Animal nutritionists have sought alternatives to formulate more efficient diets and reduce production costs and environmental concerns. In general, element excretions may be reduced by avoiding the overfeeding of specific elements or using nutritional approaches to improve element utilizations by the animals. Several nutritional strategies are possible for minimizing N, P, and trace mineral excretions： 1） to accurately meet dietary N, P and trace mineral requirements of animals, which includes reducing the dietary crude protein contents with synthetic amino acids or feeding high rumenally undegraded protein, minimizing the adequate levels of dietary P and trace elements, adopting phase or group-feeding program, and considering the bioavailable trace mineral contents in the feed ingredients; 2） to improve the bioavailabilities of dietary N, P, and trace elements for animals by using some additives（enzymes, growth promoters, probiotics, prebiotics, vitamin D isomers, and organic acids）; 3） to use highly available P sources or organic trace elements. In the future, nutrient strategies must be integrated into total production systems so that animal production systems are environmentally safe as well as economically viable.展开更多
Primary canalicular bile undergoes a process of fluidization and alkalinization along the biliary tract that is influenced by several factors including hormones, innervation/neuropeptides, and biliary constituents. Th...Primary canalicular bile undergoes a process of fluidization and alkalinization along the biliary tract that is influenced by several factors including hormones, innervation/neuropeptides, and biliary constituents. The excretion of bicarbonate at both the canaliculi and the bile ducts is an important contributor to the generation of the so-called bile-salt independent flow. Bicarbonate is secreted from hepatocytes and cholangiocytes through parallel mechanisms which involve chloride efflux through activation of Cl- channels, and further bicarbonate secretion via AE2/SLC4A2-mediated Cl-/HCO3- exchange. Glucagon and secretin are two relevant hormones which seem to act very similarly in their target cells （hepatocytes for the former and cholangiocytes for the latter）. These hormones interact with their specific G protein-coupled receptors, causing increases in intracellular levels of cAMP and activation of cAMP-dependent Cl- and HCO3- secretory mechanisms. Both hepatocytes and cholangiocytes appear to have cAMP-responsive intracellular vesicles in which AE2/SLC4A2 colocalizes with cell specific Cl- channels （CFTR in cholangiocytes and not yet determined in hepatocytes） and aquaporins （AQP8 in hepatocytes and AQP1 in cholangiocytes）, cAMP-induced coordinated trafficking of these vesicles to either canalicular or cholangiocyte lumenal membranes and further exocytosis results in increased osmotic forces and passive movement of water with net bicarbonate-rich hydrocholeresis.展开更多
AIM: To investigate the effect of albuminuria on diabetic macular edema(DME) and the possible association between baseline urinary albumin excretion(UAE) and intravitreal conbercept(IVC) treatment frequency in DME pat...AIM: To investigate the effect of albuminuria on diabetic macular edema(DME) and the possible association between baseline urinary albumin excretion(UAE) and intravitreal conbercept(IVC) treatment frequency in DME patients. METHODS: In this hospital-based retrospective study, a total of 350 in-patients with type 2 diabetes mellitus were recruited and their clinical records were reviewed. Thereafter, 52 patients identified with severe non-proliferative diabetic retinopathy(NPDR) combined with albuminuria were divided into the microalbuminuria(UAE 30-300 mg/24 h) and macroalbuminuria(UAE>300 mg/24 h) groups, which were compared and analyzed by both independent sample t-test and Chi-square test. Correlations between the systemic variables and the central foveal thickness(CFT) were evaluated using Spearman’s correlation and linear regression analyses. Of the 52 patients with centerinvolved DME, 43 received an initial combined injection of conbercept(0.5 mg/0.05 mL) and triamcinolone acetonide(1 mg/0.05 mL), followed by an IVC injection, as needed. The relationship between baseline UAE and number of IVCinjections during the first year of treatment was analyzed using Spearman’s partial correlation.RESULTS: Of 350 patients, a higher incidence of DME was observed in severe non-proliferative retinopathy(NPDR) patients than that observed in other groups. By dividing the 52 patients with severe NPDR into the micro-and macro-albuminuria subgroups, significant differences in CFT, systolic blood pressure, total cholesterol and serum creatinine levels, and UAE were revealed. Furthermore, a positive liner correlation between the UAE and CFT was found. Finally, the partial correlation coefficient adjusted for either the CFT or UAE indicated that both parameters directly correlated with the number of IVC injections administered during the 12 mo of follow-up. CONCLUSION: Generally, macular edema occurred in patients with severe NPDR, for whom the UAE is an independent risk predictor of DME. The baseline UAE and CFT predicted the treatment frequency of IVC injections administered in the first year for eyes with DME.展开更多
We evaluated the effects of salinity and body mass on the oxygen consumption rate and ammonia excretion rate of mudskipper Boleophthalmus pectinirostris under laboratory conditions. Salinity and body mass had highly s...We evaluated the effects of salinity and body mass on the oxygen consumption rate and ammonia excretion rate of mudskipper Boleophthalmus pectinirostris under laboratory conditions. Salinity and body mass had highly significant effects on the oxygen consumption rate （Ro） and ammonia excretion rate （RN） （P〈0.01）. The interactive effects between salinity and body mass on Ro and RN were insignificant （P〉0.05） and highly significant （P〈0.01）, respectively. Ro and RN of B. pectinirostris decreased significantly as the individual body mass increased. The relationship between Ro and body mass was represented by Ro=aWb （R^2=0.956, P〈0.01）. The relationship between RN and the body mass ofB. pectinirostris was represented by RN-cW^at （R^2=0.966, P〈0.01）. The Ro/RN （O：N） ratios increased significantly as the salinity increased from 12 to 27, but decreased as salinity increased from 27 to 32. The atomic O：N ratios were significantly higher at 27 than at other salinity levels. The average O：N ratio was 25.25. Lipid and carbohydrate were the primary energy sources and protein was the secondary energy significantly higher at 27 than at other salinity levels B, pectinirostris is 27. source within the salinity range 12 32. Ro andRN were Our results suggest that the optimum salinity level for B. pectinirostris is 27.展开更多
Effects of reduced salinity on the oxygen consumption rate (OCR) and the ammonia N excretion rate (AER) of scallop Chlamys farreri (3.2 5.9 cm in shell height, 0.147 1.635 g in soft tissue dry weight ) were studied in...Effects of reduced salinity on the oxygen consumption rate (OCR) and the ammonia N excretion rate (AER) of scallop Chlamys farreri (3.2 5.9 cm in shell height, 0.147 1.635 g in soft tissue dry weight ) were studied in laboratory from March 21,1997 to April 16, 1997. Under the controlled conditions of reduced salinity from 31.5 to 15.0 and ambient temperature 17℃ and 23℃, the concentrations of dissolved oxygen and ammonia N were determined by the Winkle method and the hypobromite method, respectively. Results showed that with controlled reduced salinity, the mean values of the OCR were 2.17 mg/(g.h) at 17℃, and 2.86 mg/(g.h) at 23℃and that the mean values of the AER were 178.0 μg/(g.h) at 17℃ and 147.0 μg/(g.h) at 23℃. The OCR and the AER decreased with reducing salinity from 31.5 to 15.0 both at 17℃ and 23℃. The effects of reduced salinity on the OCR and the AER of scallop C. farreri could be represented by the allometric equation and the exponential equation, respectively.展开更多
As an important bis-benzylisoquinoline alkaloid isolated from the bulbous root ofStephania tetrandra S. Moore, tetrandrine （Tet） is widely used for the treatment of malignant tumor due to its properties of reversing...As an important bis-benzylisoquinoline alkaloid isolated from the bulbous root ofStephania tetrandra S. Moore, tetrandrine （Tet） is widely used for the treatment of malignant tumor due to its properties of reversing the multidrug resistance and apoptosis induction. In the present study, we aimed to evaluate the pharmacokinetics, tissue distribution and excretion of Tet in rats. Drug concentration in plasma and tissues was measured by high performance liquid chromatography （HPLC）, and the experimental data were analyzed using pharmacokinetic software DAS 2.0. The results showed that the plasma protein binding rate of Tet was 68.7%, indicating a higher protein binding drug. Tissue distribution was found in a descending order as follows： lung〉heart〉liver〉kidney〉spleen. Renal excretion was a major route of excretion, and the urine, bile and fecal excretion accounted for 25.73% of the administered dose. A UC0-∞ of Tet in the liver was 20 times greater than that in plasma, indicating that Tet had a higher affinity for the liver. Moreover, CL in the liver was the lowest among all tissues, indicating that Tet with slow elimination might result in the accumulation. Therefore, we need to adjust the dose for patients who have dysfunction in liver and kidney. In addition, therapeutic drug monitoring in long-term clinical treatment, if necessary, should be carried out.展开更多
OBJECTIVE Eurycoma longifolia is a tropical medicinal plant belonging to Simaroubaceae distributed in South East Asia.The aim of this study is to explore the effect and mechanism of E.longifolia stem 70%ethanol extrac...OBJECTIVE Eurycoma longifolia is a tropical medicinal plant belonging to Simaroubaceae distributed in South East Asia.The aim of this study is to explore the effect and mechanism of E.longifolia stem 70%ethanol extract(EL)and its active com⁃poundson uric acid excretion.METHODS Potassium oxonate(PO)induced hyperuricemia rats and adenine-PO induced hyperuricemia mouse model were used to evaluate the effects of EL.Ultra Performance Liquid Chromatography was used to determine the levels of plasma or serum uric acid and creatinine.Hematoxylin-eosin staining was applied to observe kidney pathological changes,Western blot⁃ting was applied to detect protein expression levels of uric acid transporters.Effects of constituents on urate uptake were tested in hU⁃RAT1-expressing HEK293T cells.RESULTS EL significantly reduced serum and plasma uric acid levels at dosages of 100,200 and 400 mg·kg^-1 in hyperuricemia rats and mice,and increased the clearance rate of uric acid and creatinine,improved therenal pathological injury.The protein expression levels of urate reabsorption transporter 1(URAT1)and glucose transporter 9 were down-regulated while sodium-dependent phosphate transporter 1 and ATP-binding cassette transporter G2 were up-regulated in the kidney after EL treat⁃ment.The diterpenes(50μmol·L^-1)isolated from EL showed inhibitory effects on urate uptake in hURAT1-expressing HEK293T cells,and the effect of eurycomanol was further confirmed in vivo.CONCLUSION EL significantly reduced blood uric acid levels and prevented pathological changes of kidney in PO induced hyperuricemia animal model,improved renal urate transports.We partly clarified the mechanism was related to suppressing effect of URAT1 by diterpene in EL.This study is the first to demonstrate that EL plays a role in hyperuricemia by promoting renal uric acid excretion.展开更多
AIM: To study the stereoselectivity of phase Ⅱ glucuronidation metabolism of side-chain propranolol in Chinese Han population. METHODS: Sixteen adult Chinese Han volunteers with an average age of 20 years were given ...AIM: To study the stereoselectivity of phase Ⅱ glucuronidation metabolism of side-chain propranolol in Chinese Han population. METHODS: Sixteen adult Chinese Han volunteers with an average age of 20 years were given a single oral dose of 20 mg racemic propranolol. Human urine at indicated time after administration was collected and S-(-)-propranolol glucuronide and R-(+)-propranolol glucuronide were determined simultaneously by using RP-HPLC. RESULTS: The mean values of k were 0.19±0.04 h-1 and 0.28±0.06 h-1, of t1/2 3.56±0.73 h and 2.45±0.50 h, of Tmax 2.21±0.45 and 1.75±0.33 h, and of Xu0-24 5.65±0.98 and 2.95±0.62 μmoL for S-(-)- and R-(+)-propranolol glucuronide, respectively. The cumulative excretion percentages in urine of closes were 14.7±2.46% and 7.68±1.60% for S-(-)-and R-(+)-propranolol glucuronide, respectively. The results showed the elimination rate constant k of S-(-)-propranolol glucuronide was less than that of R-(+)-propranolol glucuronide; and the elimination half-life (t1/2), Tmax and the cumulative excretion amount (Xu0-24) of R-(+)-propranolol glucuronide were significantly less than that of S-(-)-propranolol glucuronide. CONCLUSION: The propranolol glucuronidation of the side-chain undergoes stereoselective excretion in Chinese Han population after an oral administration of racemic propranolol.展开更多
BACKGROUND Life-long removal of gluten from the diet is currently the only way to manage celiac disease(CeD).Until now,no objective test has proven useful to objectively detect ingested gluten in clinical practice.Rec...BACKGROUND Life-long removal of gluten from the diet is currently the only way to manage celiac disease(CeD).Until now,no objective test has proven useful to objectively detect ingested gluten in clinical practice.Recently,tests that determine consumption of gluten by assessing excretion of gluten immunogenic peptides(GIP)in stool and urine have been developed.Their utility,in comparison with conventional dietary and analytical follow-up strategies,has not been fully established.AIM To assess the performance of enzyme-linked immunosorbent assay(ELISA)and point-of-care tests(PoCTs)for GIP excretion in CeD patients on gluten-free diet(GFD).METHODS We conducted an observational,prospective,cross-sectional study in patients following a GFD for at least two years.Using the Gastrointestinal Symptom Rating Scale questionnaire,patients were classified at enrollment as asymptomatic or symptomatic.Gluten consumption was assessed twice by 3-d dietary recall and GIP excretion(by ELISA in stool and PoCTs(commercial kits for stool and urine)in two consecutive samples.These samples and dietary reports were obtained 10 day apart one from the other.Patients were encouraged to follow their usual GFD during the study period.RESULTS Forty-four patients were enrolled,of which 19(43.2%)were symptomatic despite being on a GFD.Overall,83 sets of stool and/or urine samples were collected.Eleven out of 44 patients(25.0%)had at least one positive GIP test.The occurrence of at least one positive test was 32%in asymptomatic patients compared with 15.8%in symptomatic patients.GIP was concordant with dietary reports in 65.9%of cases(Cohen′s kappa:0.317).PoCT detected dietary indiscretions.Both ELISA and PoCT in stool were concordant(concomitantly positive or negative)in 67 out of 74(90.5%)samples.Excretion of GIP was detected in 7(8.4%)stool and/or urine samples from patients considered to be strictly compliant with the GFD by dietary reports.CONCLUSION GIP detects dietary transgressions in patients on long-term GFD,irrespective of the presence of symptoms.PoCT for GIP detection constitutes a simple homebased method for self-assessment of dietary indiscretions.展开更多
There are more than 2000 species of brittle stars in the world. For most of them, many scientific questions including basic characteristics of eco-physiology are still unknown. In the present study, Ophiopholis sarsii...There are more than 2000 species of brittle stars in the world. For most of them, many scientific questions including basic characteristics of eco-physiology are still unknown. In the present study, Ophiopholis sarsii vadicola acclimated at 15 ℃, salinity 31, were assessed for temperature monia excretion were studied at different temperatures （5, 10, and salinity tolerance. Its oxygen consumption and am- 15, 20, 25℃） and salinities （25, 30, 35）. O. sarsii vadi- cola could tolerate 0-24℃ and no brittle star was dead in the salinity range of 19-48 in the experimental situation. Two-way ANOVA showed that the oxygen consumption and ammonia excretion normalized with both dry mass and wet mass, Q10, which is used to describe the temperature sensitivity of respiration, and moisture content were significantly affected by temperature and salinity, and the combined effects of the two factors were significant. Stepwise multiple regression analysis revealed that logarithmic oxygen consumption and ammonia excretion showed a significant positive relationship with logarithmic temperature and salinity. The logarithmic moisture content of the brittle stars showed an inverse relationship with logarithmic salinity, but a positive relationship with logarithmic temperature. This suggests that the tolerance of temperature and salinity of brittle stars is closely related to their living environment, and that the effects of temperature on oxygen consumption are more significant at higher salinity, and that the ammonia excretion is less affected by salinity at lower temperatures.展开更多
AIM: To investigate the tissue distribution, urinary and fecal excretions of 125I-lidamycin (125I-C-1027) in mice and its biliary excretion in rats. METHODS:The total radioactivity assay (RA method) and the radioactiv...AIM: To investigate the tissue distribution, urinary and fecal excretions of 125I-lidamycin (125I-C-1027) in mice and its biliary excretion in rats. METHODS:The total radioactivity assay (RA method) and the radioactivity assay after precipitation with 200 mL/L trichloroacetic add (TCA-RA method) were used to dete-rmine the tissue distribution,and the urinary and fecal excretions of 125I-C-1027 in mice and its biliary excretion in rats. RESULTS:Tissue concentrations reached the peak at the fifth minute after administration of 125I-C-1027 to mice. The highest concentration was in kidney, and the lowest in brain at all test-time points. The organs of the concentrations of 125I-C-1027 from high to low were kidney, lung, liver, stomach, spleen, uterus, ovary, intestine, muscle, heart, testis, fat, and brain in mice. The accumulative excretion amounts of 0-24 h, and 0-96 h after administration of 125I-C-1027 were 68.36 and 71.64% in urine, and 2.60 and 3.21% in feces of mice, respectively, and the accumulative excretion amount of 0-24 h was 3.57% in bile in rats. CONCLUSION: Our results reflect the characteristics of the tissue distribution, urinary and fecal excretions of 125I-C-1027 in mice and the biliary excretion of 125I-C-1027 and its metabolites in rats, and indicate that 125I-C-1027 and its metabolites are mainly distributed in kidney, and excreted in urine.展开更多
基金funded by UK Research and Innovation(UKRI)doctoral training grant no:BB/T008776/1the Department of Agriculture,Environment and Rural Affairs(DAERA)by Trouw Nutrition and by John Thompsons and Sons Ltd.
文摘Background Nitrogen-Use-Efficiency(NUE)in lactating dairy cows,defined as milk nitrogen(N)output as a proportion of N consumed,is low,with the majority of excess N excreted in manure.Excreted N can be lost to the environment as ammonia gas leading to environmental acidification and nutrient enrichment of sensitive habitats,and to watercourses contributing to aquatic eutrophication.While there is much evidence that NUE can be improved by reducing the crude protein(CP)content of dairy cow diets,the long-term impacts of feeding lower protein diets on cow performance and the rumen microbiome are less well understood.This study examined the effects of reducing the CP contents of dairy cow diets on cow performance,NUE,the relationship between NUE and residual feed intake(RFI),and the rumen microbiome.Results Dietary CP content did not affect feed intake,milk yield or milk composition(P>0.05),except for milk urea N(MUN),which increased with increasing diet CP content(P<0.05).The mean NUE was 34%,34%and 31%for the LCP(low-protein,15%),MCP(medium-protein,16%),and HCP(high-protein,17%)diets,respectively.RFI was negatively correlated with NUE(r=−0.57,P<0.001).The rumen ammonia-N concentrations increased with increasing dietary CP;however,the ruminal pH and volatile fatty acid(VFA)content of the rumen fluid remained constant.Predicted urinary N excretion was greater in the HCP and MCP diets than in the LCP diet.Reducing dietary CP content in dairy cow diets did not affect microbial composition,diversity and functional profiles.The family Bacteroidaceae was more abundant in HE(high-efficiency)cows,whereas the Methanobacteriaceae and the genus Methanobrevibacter were more abundant in LE(low-efficiency)cows.Additionally,propanoate metabolism,cysteine and methionine metabolism and amino acid biosynthesis pathways were more abundant in HE cows,whilst the methane(CH4)metabolism pathway was upregulated in LE cows.Conclusions The results demonstrate that diet CP can be reduced with no loss in cow performance,but with an associated reduction in N excretion.The abundance of microbial populations differed between low and high efficiency cows,which may contribute to the differences in efficiency observed.
基金National Innovation and Entrepreneurship Training Program for College Students(No.S202010823014)Hunan Provincial College Student Innovation Training Project,No.(2021)199(S202110823045)。
文摘Objective: To analyze the possible mechanism of Pueraria isoflavones inhibiting XOD and GLUT9 to reduce uric acid production and promote uric acid excretion. Methods: August 2021-April 2022, a total of forty SPF male Kunming mice were divided into the healthy group (carboxymethylcellulose sodium at a dose of 250 mg/kg), the model group (HUA mice were given carboxymethylcellulose sodium at a dose of 250 mg/kg), the low group (HUA mice were given pueraria isoflavone at a dose of 125 mg/kg), HUA mice were given pueraria isoflavones at a dose of 250 mg/kg once d frequency)and the high group (HUA mice were given pueraria isoflavones at a dose of 500 mg/kg once d frequency) dosage groups, with 8 mice in each group. The contents of uric acid (SUA), urea nitrogen (BUN) and creatinine (SCr) in serum and urine of each group were compared before and after intervention (30 d). Statistical differences of xanthine oxidase (XOD) and human glucose transporter 9(GLUT9), cy- clooxygenase- 2(COX-2), tumor necrosis factor (TNF-α) and interleukin-1 (IL-1β) contents in renal tissues of each group after intervention (30 d) were compared. Results: After intervention, kidney inflammatory factors (COX-2, TNF-α and IL-1β) in the model group were compared. Blood and urine indexes (SUA, BUN, SCr);The contents of XOD and GLUT9 were higher than those of healthy group(P<0.05). Renal inflammatory cytokines (COX-2, TNF-α and IL-1β) in low, medium and high dose groups;Blood and urine indexes (SUA, BUN, SCr);The contents of XOD and GLUT9 were lower than those of model group, and there were low > medium > high dose groups, the comparison between the two groups had statistical significance(P< 0.05). After intervention, the contents of 3 indicators in blood or urine(COX-2, TNF-α and IL-1β) all decreased compared with before intervention, and the differences in intra-group comparison were statistically significant (P<0.05). Conclusion: Pueraria isoflavones can treat HUA mice by inhibiting the expression of XOD and GLUT9, and then play a role in reducing uric acid pro- duction and promoting uric acid excretion, as well as alleviating the degree of disease inflammation.
文摘Biofouling is a particular problem in the pearl oyster culture. It may reduce the growth and survival rates of the cultured species. Foulers are often themselves filter feeders, and are therefore potential competitors for food resource with the cultured species. Fouling organisms may also reduce the oxygen supply. A study was conducted to measure the impact of foulers on feeding oxygen consumption, and waste excretion. POM, ammonia, phosphate and oxygen concentration were measured in various treatments （cultured species, foulers）. This study showed that fouling organisms had significant effect on food uptake, oxygen consumption and waste excretion. The clearance rate, ammonia and phosphate release rate, oxygen consumption rate of the fouled pearl oyster were greater than those of the clean pearl oysters. Other foulers that settled on cages or buoys also contributed much to phytoplankton depletion, oxygen consumption and concentration increase of ammonia and phosphate in water. Therefore, this study showed us that foulers were important competitors in the pearl oyster cultivation of Daya Bay in November 2005.
文摘AIMTo determine the accuracy of fractional excretion of sodium (FeNa) in the diagnosis of hepatorenal syndrome (HRS). METHODSEighty-eight liver transplantation candidates with renal dysfunction and/or proteinuria were included in the study sample. The baseline characteristics of the patients were obtained. All the 88 patients underwent iothalamate glomerular filtration rate testing, 24-h urine collection for urinary sodium and protein excretions, random urine for sodium and creatinine testing, and percutaneous kidney biopsy. FeNa was calculated using the equation [(urine sodium × serum creatinine)/(serum sodium × urine creatinine)] × 100%. Diuretic use was recorded among the participants. Patients on renal replacement therapy were not included in the original sample. RESULTSSeventy-seven (87%) of the 88 patients had FeNa P = 0.4). FeNa P = 0.47). Calculated positive predictive value and negative predictive value for FeNa P = 0.41). CONCLUSIONFeNa 1%.
基金The Fundamental Research Funds for the Central Universities(Grant No.JKZ2011016)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘The present study aimed at studying the characteristics of tissue distribution and excretion of scopoletin,a coumarin compound,in Sprague-Dawley rats.Scopoletin was orally administered at a dose of 50 mg/kg,and the concentrations in heart,liver,spleen,lung,kidney,muscle,fat,brain,testis,uterus,stomach and small intestine were determined at 5,15,30,60,120,240 min post-dose,respectively.It was shown that scopoletin was widely distributed into various tissues and reached the maximal concentrations in most tissues at 15 min post-dose,and the levels in liver,kidney,stomach and small intestine were relatively higher.Furthermore,the excretions of scopoletin in bile,urine and feces were only 0.032%,3.752% and 0.784%,respectively,suggesting that scopoletin was mainly eliminated by metabolism rather than excretion as parent drug.
文摘The massive COVID-19 vaccination campaign is the first time that mRNA vaccines have been used on a global scale.The mRNA vaccines correspond exactly to the definition of gene therapy of the American and European regulatory agencies.The regulations require excretion studies of these drugs and their products(the translated proteins).These studies have not been done for mRNA vaccines(nor for adenovirus vaccines).There are numerous reports of symptoms and pathologies identical to the adverse effects of mRNA vaccines in unvaccinated persons in contact with freshly vaccinated persons.It is therefore important to review the state of knowledge on the possible excretion of vaccine nanoparticles as well as mRNA and its product,the spike protein.Vaccine mRNA-carrying lipid nanoparticles spread after injection throughout the body according to available animal studies and vaccine mRNA(naked or in nanoparticles or in natural exosomes)is found in the bloodstream as well as vaccine spike in free form or encapsulated in exosomes(shown in human studies).Lipid nanoparticles(or their natural equivalent,exosomes or extracellular vesicles(EVs))have been shown to be able to be excreted through body fluids(sweat,sputum,breast milk)and to pass the transplacental barrier.These EVs are also able to penetrate by inhalation and through the skin(healthy or injured)as well as orally through breast milk(and why not during sexual intercourse through semen,as this has not been studied).It is urgent to enforce the legislation on gene therapy that applies to mRNA vaccines and to carry out studies on this subject while the generalization of mRNA vaccines is being considered.
基金supported by the Grant from the Science and Technology Support Program of Sichuan Province(2015NZ0042,2016NZ006)National Key R&D Program of China(2018YFD0500605).
文摘Background:This study was conducted to determine the effects of different dietary protein levels and amino acids supplementation patterns in low protein diets on the growth performance,carcass characteristics and nitrogen excretion in growing-finishing pigs.Forty-two barrows(25.00±0.39 kg)were randomly assigned to 7 diets.Diet 1:the high crude protein diet with balanced for 10 essential amino acids(EAAs).Diet 2:the medium crude protein diet with 2%(approx)decreased protein level of Diet 1 and balanced 10 EAAs.Diet 3:the low crude protein diet with 4%decreased protein level of Diet 1 and balanced 10 EAAs.The protein levels of Diet 4,5,6 and 7 were the same as that of Diet 3.Diet 4 was only balanced for lysine(Lys),methionine(Met),threonine(Thr)and tryptophan(Trp);Diet 5 and 6 were further supplemented with extra isoleucine(Ile)or valine(Val),respectively;Diet 7 was further supplemented with extra Ile+Val.Results:Over the 112 days trial,the reduction of dietary protein by 2%or 4%with balanced10 EAAs significantly decreased nitrogen excretion(P<0.05),but had no effects on growth performance and carcass characteristics(P>0.05).In low protein diet,Val supplementation significantly increased body weight gain at 25–50 kg phase(P<0.05),while Ile supplementation at 75–100 kg phase and 100–125 kg phase significantly reduced the ratio of feed to gain(P<0.05).No effect of different dietary protein levels and amino acids supplementation patterns in low protein diets on carcass characteristics was observed(P>0.05).The total N excretion of pigs supplemented with only Lys,Met,Thr and Trp was numerically higher than that of pigs fed with extra Ile,or Val,or Ile+Val diets.Conclusion:In low protein diet,Val is more required than Ile in the early growing phage(25–50 kg),while Ile becomes more required in the late growing and finishing phage(75–125 kg).
基金This work is supported by National High-Tech R & D Program of China,(863 Program) (2002AA603014)
文摘Effects of temperatures and salinities on oxygen consumption and ammonia-N excretion rate of clam Meretrix meretrix were studied in laboratory from Oct. 2003 to Jan. 2004. Two schemes were designed in incremented temperature at 10, 15, 20, 25℃ at 31.5 salinity and in incremented salinity at16.0, 21.0, 26.0, 31.5, 36.0, and 41.0 at 20℃, all for 8-10 days. From 10 to 25℃, both respiration and excretion rate were increased. One-way ANOVA analysis demonstrated significant difference （P〈0.01） in physiological parameters in this temperature range except between 15 and 20℃. The highest Q10 thermal coefficient value （12.27） was acquired between 10 and 15℃, and about 1 between 15 and 20℃, indicating M. meretrix could well acclimate to temperature changes in this range. Salinity also had significant effects on respiration and excretion rate （P〈0.05）. The highest values of respiration and excretion rate of M. meretrix were recorded at 16.0 salinity （20℃）. These two physiological parameters decreased as salinity increased until reached the minimum Q10 value at 31.5 （20℃）, then again, these parameters increased with increasing salinity from 31.5 to 41.0. M. meretrix can catabolize body protein to cope with osmotic pressure stress when environmental salinity is away from its optimal range. No significant difference was observed between 26.0 and 36.0 in salinity （P〉0.05）, suggesting that a best metabolic salinity range for this species is between 26.0 and 36.0.
基金supported by the Key Program of Transgenic Plant Breeding,China (2008ZX08003-002)the Major Special Project of Guangdong Province,China(2009A080303009)+1 种基金the National Major Science Research Program of China (2009CB941601)the Special Fund for Agro-Scientific Research in the Public Interest,China (Agriculture,201003011)
文摘Two experiments were conducted to investigate the effect of dietary supplementation with phytase transgenic corn （PTC） on growth performance,phosphorus （P） utilization and excretion in growing pigs.In Exp.1,180 pigs （Large White × Landrace,BW=37.7 kg） were randomly allotted to 4 treatments with 5 replicates of 9 pigs each in order to evaluate the effect of PTC supplementation in low-P diets on growth performance.Four corn soybean meal-based diets consisted of a positive control （PC） diet,a diet containing 500 units （U） of exogenous phytase kg-1 （EP） on the basis of low-P （inorganic P reduced by 0.05% from PC diet） and the low-P＋500 （PTC1） or 750 （PTC2） phytase U of PTC kg-1.In Exp.2,20 barrows （Large White×Landrace,BW=31 kg,4 treatments with 5 replicates of 1 pig each） were randomly selected to evaluate the effect of PTC in low-P diets on serum parameters and nutrient utilization.Diets in Exp.2 were similar to those in Exp.1 except that the EP group was replaced by a low-P diet without exogenous phytase supplementation as a negative control （NC） group.The results from Exp.1 showed that the average daily gain （ADG） in the PTC2 group was significantly higher （P〈0.05） than that in the EP group over all periods.On the other hand,the feed：gain （F：G） ratio of the EP group was significantly higher （P〈0.05） than that of the PTC2 group during 1-21 and 1-42 d,respectively.There were no differences in average daily feed intake （ADFI） among all treatments （P〉0.05）.The results from Exp.2 showed that the concentration of serum Ca in the NC group was the highest （P〈0.05）,while the concentration of serum P in the PTC2 group was the highest （P〈0.05） among all treatments.There was a significant decrease （P〈0.05） in the P apparent digestibility of the NC group compared with the other groups,and that of PTC2 group was the best.Furthermore,fecal P excretion was reduced （P〈0.05） from 1.80 g d-1 in the PC group to 1.28 g d-1 in the PTC2 group.In conclusion,dietary supplementation with PTC could reduce the application of inorganic P,decrease fecal P excretion,and improve the growth performance of growing pigs.
基金supported by the Key Program of the National Natural Science Foundation of China (31630073)the Program of the National Natural Science Foundation of China (31472116 and 31672440)+2 种基金the Program of Student Community of Professor Yang Sheng (B2016013)the Agricultural Science and Technology Innovation Program (ASTIP-IAS08)the earmarked fund for China Agriculture Research System (CARS-41)
文摘Animal agriculture contributes to environmental pollutions through the surplus nitrogen（N）, phosphorus（P）, and trace minerals that animals excrete. Animal nutritionists have sought alternatives to formulate more efficient diets and reduce production costs and environmental concerns. In general, element excretions may be reduced by avoiding the overfeeding of specific elements or using nutritional approaches to improve element utilizations by the animals. Several nutritional strategies are possible for minimizing N, P, and trace mineral excretions： 1） to accurately meet dietary N, P and trace mineral requirements of animals, which includes reducing the dietary crude protein contents with synthetic amino acids or feeding high rumenally undegraded protein, minimizing the adequate levels of dietary P and trace elements, adopting phase or group-feeding program, and considering the bioavailable trace mineral contents in the feed ingredients; 2） to improve the bioavailabilities of dietary N, P, and trace elements for animals by using some additives（enzymes, growth promoters, probiotics, prebiotics, vitamin D isomers, and organic acids）; 3） to use highly available P sources or organic trace elements. In the future, nutrient strategies must be integrated into total production systems so that animal production systems are environmentally safe as well as economically viable.
基金the "UTE for CIMA project" as well as by a grant from the "Institute de Salud CarlosⅢ" (PI051098). J. M. B. has a grant from the Spanish Ministry of Science and Technology
文摘Primary canalicular bile undergoes a process of fluidization and alkalinization along the biliary tract that is influenced by several factors including hormones, innervation/neuropeptides, and biliary constituents. The excretion of bicarbonate at both the canaliculi and the bile ducts is an important contributor to the generation of the so-called bile-salt independent flow. Bicarbonate is secreted from hepatocytes and cholangiocytes through parallel mechanisms which involve chloride efflux through activation of Cl- channels, and further bicarbonate secretion via AE2/SLC4A2-mediated Cl-/HCO3- exchange. Glucagon and secretin are two relevant hormones which seem to act very similarly in their target cells （hepatocytes for the former and cholangiocytes for the latter）. These hormones interact with their specific G protein-coupled receptors, causing increases in intracellular levels of cAMP and activation of cAMP-dependent Cl- and HCO3- secretory mechanisms. Both hepatocytes and cholangiocytes appear to have cAMP-responsive intracellular vesicles in which AE2/SLC4A2 colocalizes with cell specific Cl- channels （CFTR in cholangiocytes and not yet determined in hepatocytes） and aquaporins （AQP8 in hepatocytes and AQP1 in cholangiocytes）, cAMP-induced coordinated trafficking of these vesicles to either canalicular or cholangiocyte lumenal membranes and further exocytosis results in increased osmotic forces and passive movement of water with net bicarbonate-rich hydrocholeresis.
基金Supported by Nature Science Foundation of Shaanxi Province of China (No.2017JM8074)
文摘AIM: To investigate the effect of albuminuria on diabetic macular edema(DME) and the possible association between baseline urinary albumin excretion(UAE) and intravitreal conbercept(IVC) treatment frequency in DME patients. METHODS: In this hospital-based retrospective study, a total of 350 in-patients with type 2 diabetes mellitus were recruited and their clinical records were reviewed. Thereafter, 52 patients identified with severe non-proliferative diabetic retinopathy(NPDR) combined with albuminuria were divided into the microalbuminuria(UAE 30-300 mg/24 h) and macroalbuminuria(UAE>300 mg/24 h) groups, which were compared and analyzed by both independent sample t-test and Chi-square test. Correlations between the systemic variables and the central foveal thickness(CFT) were evaluated using Spearman’s correlation and linear regression analyses. Of the 52 patients with centerinvolved DME, 43 received an initial combined injection of conbercept(0.5 mg/0.05 mL) and triamcinolone acetonide(1 mg/0.05 mL), followed by an IVC injection, as needed. The relationship between baseline UAE and number of IVCinjections during the first year of treatment was analyzed using Spearman’s partial correlation.RESULTS: Of 350 patients, a higher incidence of DME was observed in severe non-proliferative retinopathy(NPDR) patients than that observed in other groups. By dividing the 52 patients with severe NPDR into the micro-and macro-albuminuria subgroups, significant differences in CFT, systolic blood pressure, total cholesterol and serum creatinine levels, and UAE were revealed. Furthermore, a positive liner correlation between the UAE and CFT was found. Finally, the partial correlation coefficient adjusted for either the CFT or UAE indicated that both parameters directly correlated with the number of IVC injections administered during the 12 mo of follow-up. CONCLUSION: Generally, macular edema occurred in patients with severe NPDR, for whom the UAE is an independent risk predictor of DME. The baseline UAE and CFT predicted the treatment frequency of IVC injections administered in the first year for eyes with DME.
基金Supported by the Natural Science Foundation of Guangdong Province(No.8152408801000015)
文摘We evaluated the effects of salinity and body mass on the oxygen consumption rate and ammonia excretion rate of mudskipper Boleophthalmus pectinirostris under laboratory conditions. Salinity and body mass had highly significant effects on the oxygen consumption rate （Ro） and ammonia excretion rate （RN） （P〈0.01）. The interactive effects between salinity and body mass on Ro and RN were insignificant （P〉0.05） and highly significant （P〈0.01）, respectively. Ro and RN of B. pectinirostris decreased significantly as the individual body mass increased. The relationship between Ro and body mass was represented by Ro=aWb （R^2=0.956, P〈0.01）. The relationship between RN and the body mass ofB. pectinirostris was represented by RN-cW^at （R^2=0.966, P〈0.01）. The Ro/RN （O：N） ratios increased significantly as the salinity increased from 12 to 27, but decreased as salinity increased from 27 to 32. The atomic O：N ratios were significantly higher at 27 than at other salinity levels. The average O：N ratio was 25.25. Lipid and carbohydrate were the primary energy sources and protein was the secondary energy significantly higher at 27 than at other salinity levels B, pectinirostris is 27. source within the salinity range 12 32. Ro andRN were Our results suggest that the optimum salinity level for B. pectinirostris is 27.
文摘Effects of reduced salinity on the oxygen consumption rate (OCR) and the ammonia N excretion rate (AER) of scallop Chlamys farreri (3.2 5.9 cm in shell height, 0.147 1.635 g in soft tissue dry weight ) were studied in laboratory from March 21,1997 to April 16, 1997. Under the controlled conditions of reduced salinity from 31.5 to 15.0 and ambient temperature 17℃ and 23℃, the concentrations of dissolved oxygen and ammonia N were determined by the Winkle method and the hypobromite method, respectively. Results showed that with controlled reduced salinity, the mean values of the OCR were 2.17 mg/(g.h) at 17℃, and 2.86 mg/(g.h) at 23℃and that the mean values of the AER were 178.0 μg/(g.h) at 17℃ and 147.0 μg/(g.h) at 23℃. The OCR and the AER decreased with reducing salinity from 31.5 to 15.0 both at 17℃ and 23℃. The effects of reduced salinity on the OCR and the AER of scallop C. farreri could be represented by the allometric equation and the exponential equation, respectively.
基金The Scientific Research Fund Project of Heilongjiang University of Chinese Medicine(Grant No.201408)
文摘As an important bis-benzylisoquinoline alkaloid isolated from the bulbous root ofStephania tetrandra S. Moore, tetrandrine （Tet） is widely used for the treatment of malignant tumor due to its properties of reversing the multidrug resistance and apoptosis induction. In the present study, we aimed to evaluate the pharmacokinetics, tissue distribution and excretion of Tet in rats. Drug concentration in plasma and tissues was measured by high performance liquid chromatography （HPLC）, and the experimental data were analyzed using pharmacokinetic software DAS 2.0. The results showed that the plasma protein binding rate of Tet was 68.7%, indicating a higher protein binding drug. Tissue distribution was found in a descending order as follows： lung〉heart〉liver〉kidney〉spleen. Renal excretion was a major route of excretion, and the urine, bile and fecal excretion accounted for 25.73% of the administered dose. A UC0-∞ of Tet in the liver was 20 times greater than that in plasma, indicating that Tet had a higher affinity for the liver. Moreover, CL in the liver was the lowest among all tissues, indicating that Tet with slow elimination might result in the accumulation. Therefore, we need to adjust the dose for patients who have dysfunction in liver and kidney. In addition, therapeutic drug monitoring in long-term clinical treatment, if necessary, should be carried out.
文摘OBJECTIVE Eurycoma longifolia is a tropical medicinal plant belonging to Simaroubaceae distributed in South East Asia.The aim of this study is to explore the effect and mechanism of E.longifolia stem 70%ethanol extract(EL)and its active com⁃poundson uric acid excretion.METHODS Potassium oxonate(PO)induced hyperuricemia rats and adenine-PO induced hyperuricemia mouse model were used to evaluate the effects of EL.Ultra Performance Liquid Chromatography was used to determine the levels of plasma or serum uric acid and creatinine.Hematoxylin-eosin staining was applied to observe kidney pathological changes,Western blot⁃ting was applied to detect protein expression levels of uric acid transporters.Effects of constituents on urate uptake were tested in hU⁃RAT1-expressing HEK293T cells.RESULTS EL significantly reduced serum and plasma uric acid levels at dosages of 100,200 and 400 mg·kg^-1 in hyperuricemia rats and mice,and increased the clearance rate of uric acid and creatinine,improved therenal pathological injury.The protein expression levels of urate reabsorption transporter 1(URAT1)and glucose transporter 9 were down-regulated while sodium-dependent phosphate transporter 1 and ATP-binding cassette transporter G2 were up-regulated in the kidney after EL treat⁃ment.The diterpenes(50μmol·L^-1)isolated from EL showed inhibitory effects on urate uptake in hURAT1-expressing HEK293T cells,and the effect of eurycomanol was further confirmed in vivo.CONCLUSION EL significantly reduced blood uric acid levels and prevented pathological changes of kidney in PO induced hyperuricemia animal model,improved renal urate transports.We partly clarified the mechanism was related to suppressing effect of URAT1 by diterpene in EL.This study is the first to demonstrate that EL plays a role in hyperuricemia by promoting renal uric acid excretion.
基金Supported by the National Natural Science Foundation of China, No. 30225047
文摘AIM: To study the stereoselectivity of phase Ⅱ glucuronidation metabolism of side-chain propranolol in Chinese Han population. METHODS: Sixteen adult Chinese Han volunteers with an average age of 20 years were given a single oral dose of 20 mg racemic propranolol. Human urine at indicated time after administration was collected and S-(-)-propranolol glucuronide and R-(+)-propranolol glucuronide were determined simultaneously by using RP-HPLC. RESULTS: The mean values of k were 0.19±0.04 h-1 and 0.28±0.06 h-1, of t1/2 3.56±0.73 h and 2.45±0.50 h, of Tmax 2.21±0.45 and 1.75±0.33 h, and of Xu0-24 5.65±0.98 and 2.95±0.62 μmoL for S-(-)- and R-(+)-propranolol glucuronide, respectively. The cumulative excretion percentages in urine of closes were 14.7±2.46% and 7.68±1.60% for S-(-)-and R-(+)-propranolol glucuronide, respectively. The results showed the elimination rate constant k of S-(-)-propranolol glucuronide was less than that of R-(+)-propranolol glucuronide; and the elimination half-life (t1/2), Tmax and the cumulative excretion amount (Xu0-24) of R-(+)-propranolol glucuronide were significantly less than that of S-(-)-propranolol glucuronide. CONCLUSION: The propranolol glucuronidation of the side-chain undergoes stereoselective excretion in Chinese Han population after an oral administration of racemic propranolol.
文摘BACKGROUND Life-long removal of gluten from the diet is currently the only way to manage celiac disease(CeD).Until now,no objective test has proven useful to objectively detect ingested gluten in clinical practice.Recently,tests that determine consumption of gluten by assessing excretion of gluten immunogenic peptides(GIP)in stool and urine have been developed.Their utility,in comparison with conventional dietary and analytical follow-up strategies,has not been fully established.AIM To assess the performance of enzyme-linked immunosorbent assay(ELISA)and point-of-care tests(PoCTs)for GIP excretion in CeD patients on gluten-free diet(GFD).METHODS We conducted an observational,prospective,cross-sectional study in patients following a GFD for at least two years.Using the Gastrointestinal Symptom Rating Scale questionnaire,patients were classified at enrollment as asymptomatic or symptomatic.Gluten consumption was assessed twice by 3-d dietary recall and GIP excretion(by ELISA in stool and PoCTs(commercial kits for stool and urine)in two consecutive samples.These samples and dietary reports were obtained 10 day apart one from the other.Patients were encouraged to follow their usual GFD during the study period.RESULTS Forty-four patients were enrolled,of which 19(43.2%)were symptomatic despite being on a GFD.Overall,83 sets of stool and/or urine samples were collected.Eleven out of 44 patients(25.0%)had at least one positive GIP test.The occurrence of at least one positive test was 32%in asymptomatic patients compared with 15.8%in symptomatic patients.GIP was concordant with dietary reports in 65.9%of cases(Cohen′s kappa:0.317).PoCT detected dietary indiscretions.Both ELISA and PoCT in stool were concordant(concomitantly positive or negative)in 67 out of 74(90.5%)samples.Excretion of GIP was detected in 7(8.4%)stool and/or urine samples from patients considered to be strictly compliant with the GFD by dietary reports.CONCLUSION GIP detects dietary transgressions in patients on long-term GFD,irrespective of the presence of symptoms.PoCT for GIP detection constitutes a simple homebased method for self-assessment of dietary indiscretions.
基金supported by the National Basic Research Program of China (Grant No. 2011CB409805)the National Science & Technology Pillar Program (Grant No. 2011BAD13B06)+1 种基金Special Scientific Research Funds for Central Non-profit Institutes, Yellow Sea Fisheries Research Institute (20603022013042)the National Science and Technology Planning Project of China (Grant No. 2011BAD13B05)
文摘There are more than 2000 species of brittle stars in the world. For most of them, many scientific questions including basic characteristics of eco-physiology are still unknown. In the present study, Ophiopholis sarsii vadicola acclimated at 15 ℃, salinity 31, were assessed for temperature monia excretion were studied at different temperatures （5, 10, and salinity tolerance. Its oxygen consumption and am- 15, 20, 25℃） and salinities （25, 30, 35）. O. sarsii vadi- cola could tolerate 0-24℃ and no brittle star was dead in the salinity range of 19-48 in the experimental situation. Two-way ANOVA showed that the oxygen consumption and ammonia excretion normalized with both dry mass and wet mass, Q10, which is used to describe the temperature sensitivity of respiration, and moisture content were significantly affected by temperature and salinity, and the combined effects of the two factors were significant. Stepwise multiple regression analysis revealed that logarithmic oxygen consumption and ammonia excretion showed a significant positive relationship with logarithmic temperature and salinity. The logarithmic moisture content of the brittle stars showed an inverse relationship with logarithmic salinity, but a positive relationship with logarithmic temperature. This suggests that the tolerance of temperature and salinity of brittle stars is closely related to their living environment, and that the effects of temperature on oxygen consumption are more significant at higher salinity, and that the ammonia excretion is less affected by salinity at lower temperatures.
基金Supported by the National High Technology Research and Development Program of China (863 Program), No. 2003AA2Z347D
文摘AIM: To investigate the tissue distribution, urinary and fecal excretions of 125I-lidamycin (125I-C-1027) in mice and its biliary excretion in rats. METHODS:The total radioactivity assay (RA method) and the radioactivity assay after precipitation with 200 mL/L trichloroacetic add (TCA-RA method) were used to dete-rmine the tissue distribution,and the urinary and fecal excretions of 125I-C-1027 in mice and its biliary excretion in rats. RESULTS:Tissue concentrations reached the peak at the fifth minute after administration of 125I-C-1027 to mice. The highest concentration was in kidney, and the lowest in brain at all test-time points. The organs of the concentrations of 125I-C-1027 from high to low were kidney, lung, liver, stomach, spleen, uterus, ovary, intestine, muscle, heart, testis, fat, and brain in mice. The accumulative excretion amounts of 0-24 h, and 0-96 h after administration of 125I-C-1027 were 68.36 and 71.64% in urine, and 2.60 and 3.21% in feces of mice, respectively, and the accumulative excretion amount of 0-24 h was 3.57% in bile in rats. CONCLUSION: Our results reflect the characteristics of the tissue distribution, urinary and fecal excretions of 125I-C-1027 in mice and the biliary excretion of 125I-C-1027 and its metabolites in rats, and indicate that 125I-C-1027 and its metabolites are mainly distributed in kidney, and excreted in urine.
