Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombin...Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production. A plasmid containing the capsid precursor P12A and protease 3C sequences of foot-and-mouth disease virus (FMDV) was constructed and used to compare transient and stable expression in mammalian cells. When BHK-21 cells were transfected with the recombinant vector, protease 3C cleaved the capsid precursor P12A into the structural proteins VP0, VP1 and VP3. A sucrose gradient demonstrated that the structural proteins assembled into different subviral particles. Attempts to generate a stable cell line only allowed isolating low-level-expressing clones, probably due to the effect of protease 3C on the cells. Moreover, the recombinant protein yield achieved in transient expression assays was much higher than the one achieved in stable expression assays. Results indicate that mammalian cells are a good strategy to produce recombinant FMDV subviral particles. However, the alternative approach of transient gene expression in scalable systems should be used instead of the standard method that involves the generation of a stable cell line.展开更多
A new method to extract person-independent expression feature based on higher-order singular value decomposition （HOSVD） is proposed for facial expression recognition. Based on the assumption that similar persons ha...A new method to extract person-independent expression feature based on higher-order singular value decomposition （HOSVD） is proposed for facial expression recognition. Based on the assumption that similar persons have similar facial expression appearance and shape, the person-similarity weighted expression feature is proposed to estimate the expression feature of test persons. As a result, the estimated expression feature can reduce the influence of individuals caused by insufficient training data, and hence become less person-dependent. The proposed method is tested on Cohn-Kanade facial expression database and Japanese female facial expression （JAFFE） database. Person-independent experimental results show the superiority of the proposed method over the existing methods.展开更多
In this paper,a set of closed-form formulas for vector Finite Element Method(FEM) to analyze three dimensional electromagnetic problems is presented on the basis of Gaussian quadrature integration scheme.By analyzing ...In this paper,a set of closed-form formulas for vector Finite Element Method(FEM) to analyze three dimensional electromagnetic problems is presented on the basis of Gaussian quadrature integration scheme.By analyzing the open region problems,the first-order Absorbing Boundary Condition(ABC) is considered as the truncation boundary condition and the equation is carried out in a closed-form.Based on the formulas,the hybrid Expanded Cholesky Method(ECM) and MultiFrontal algorithm(MF) is applied to solve finite element equations.Using the closed-form solution,the elec-tromagnetic field of three dimensional targets can be studied sententiously and accurately.Simulation results show that the presented formulas are successfully and concise,which can be easily used to analyze three dimensional electromagnetic problems.展开更多
文摘Foot-and-mouth disease is a highly contagious disease that produces severe economic losses in the livestock industry. This disease is being controlled by the use of an inactivated vaccine. However, the use of recombinant empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production. A plasmid containing the capsid precursor P12A and protease 3C sequences of foot-and-mouth disease virus (FMDV) was constructed and used to compare transient and stable expression in mammalian cells. When BHK-21 cells were transfected with the recombinant vector, protease 3C cleaved the capsid precursor P12A into the structural proteins VP0, VP1 and VP3. A sucrose gradient demonstrated that the structural proteins assembled into different subviral particles. Attempts to generate a stable cell line only allowed isolating low-level-expressing clones, probably due to the effect of protease 3C on the cells. Moreover, the recombinant protein yield achieved in transient expression assays was much higher than the one achieved in stable expression assays. Results indicate that mammalian cells are a good strategy to produce recombinant FMDV subviral particles. However, the alternative approach of transient gene expression in scalable systems should be used instead of the standard method that involves the generation of a stable cell line.
基金supported by National Natural Science Foundation of China (6087208460940008)+2 种基金Beijing Training Programming Foundation for the Talents (20081D1600300343)Excellent Young Scholar Research Fund of Beijing Institute of Technology (2007Y0305)Fundamental Research Foundation of Beijing Institute of Technology (20080342005)
文摘A new method to extract person-independent expression feature based on higher-order singular value decomposition （HOSVD） is proposed for facial expression recognition. Based on the assumption that similar persons have similar facial expression appearance and shape, the person-similarity weighted expression feature is proposed to estimate the expression feature of test persons. As a result, the estimated expression feature can reduce the influence of individuals caused by insufficient training data, and hence become less person-dependent. The proposed method is tested on Cohn-Kanade facial expression database and Japanese female facial expression （JAFFE） database. Person-independent experimental results show the superiority of the proposed method over the existing methods.
基金Supported by the National Science Foundation of China(No. 60801039)
文摘In this paper,a set of closed-form formulas for vector Finite Element Method(FEM) to analyze three dimensional electromagnetic problems is presented on the basis of Gaussian quadrature integration scheme.By analyzing the open region problems,the first-order Absorbing Boundary Condition(ABC) is considered as the truncation boundary condition and the equation is carried out in a closed-form.Based on the formulas,the hybrid Expanded Cholesky Method(ECM) and MultiFrontal algorithm(MF) is applied to solve finite element equations.Using the closed-form solution,the elec-tromagnetic field of three dimensional targets can be studied sententiously and accurately.Simulation results show that the presented formulas are successfully and concise,which can be easily used to analyze three dimensional electromagnetic problems.
