<span lang="EN-US" style="font-family:;" "="">The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. ...<span lang="EN-US" style="font-family:;" "="">The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. The efficiency in domestic cat IVM concerning <span lang="EN-US" style="font-family:;" "="">the <span lang="EN-US" style="font-family:;" "="">reproductive status and/or cultivation times has previously been investigated;however, the studies were carried out separately. The objective of this research was to evaluate the maturation of oocytes of domestic cats of different reproductive stages using two different in vitro culture times. The ovaries were obtained by Ooforo-Salpingo-Hysterectomy of cats that were of the following groups: 1) prepubertal, 2) follicular, 3) pregnant or 4) in anestrus. Maturation was carried out with TCM199 medium supplemented with BSA for 24 h and 48 h. On average, 29 ± 25, 20 ± 15, 17 ± 9 and 17 ± 13 oocytes/cat were recovered from the prepubertal follicular, pregnant<span lang="EN-US" style="font-family:;" "="">,<span lang="EN-US" style="font-family:;" "=""> and anestrus stages, respectively, but did not show a significant difference (P<span lang="EN-US" style="font-family:;" "=""> ><span lang="EN-US"> <span lang="EN-US" style="font-family:;" "="">0.05). Also, meiotic maturation did not show a significant difference between the different reproductive stages at 24 h and 48 h, respectively (P > 0.05). However, in the prepubertal and follicular stages, greater oocyte maturation numbers were observed at 48 h compared to 24 h (P < 0.05). In contrast, the aforementioned <span lang="EN-US" style="font-family:;" "="">result <span lang="EN-US" style="font-family:;" "="">was not observed in the pregnant and anestrus stages (P > 0.05), indicating that the in vitro culture duration is an important factor during in vitro maturation of domestic cat oocytes.展开更多
文摘<span lang="EN-US" style="font-family:;" "="">The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. The efficiency in domestic cat IVM concerning <span lang="EN-US" style="font-family:;" "="">the <span lang="EN-US" style="font-family:;" "="">reproductive status and/or cultivation times has previously been investigated;however, the studies were carried out separately. The objective of this research was to evaluate the maturation of oocytes of domestic cats of different reproductive stages using two different in vitro culture times. The ovaries were obtained by Ooforo-Salpingo-Hysterectomy of cats that were of the following groups: 1) prepubertal, 2) follicular, 3) pregnant or 4) in anestrus. Maturation was carried out with TCM199 medium supplemented with BSA for 24 h and 48 h. On average, 29 ± 25, 20 ± 15, 17 ± 9 and 17 ± 13 oocytes/cat were recovered from the prepubertal follicular, pregnant<span lang="EN-US" style="font-family:;" "="">,<span lang="EN-US" style="font-family:;" "=""> and anestrus stages, respectively, but did not show a significant difference (P<span lang="EN-US" style="font-family:;" "=""> ><span lang="EN-US"> <span lang="EN-US" style="font-family:;" "="">0.05). Also, meiotic maturation did not show a significant difference between the different reproductive stages at 24 h and 48 h, respectively (P > 0.05). However, in the prepubertal and follicular stages, greater oocyte maturation numbers were observed at 48 h compared to 24 h (P < 0.05). In contrast, the aforementioned <span lang="EN-US" style="font-family:;" "="">result <span lang="EN-US" style="font-family:;" "="">was not observed in the pregnant and anestrus stages (P > 0.05), indicating that the in vitro culture duration is an important factor during in vitro maturation of domestic cat oocytes.
