The mechanistic target of rapamycin(m TOR) is a serine/threonine kinase that plays a pivotal role in cellular growth, proliferation, survival, and metabolism. In the central nervous system(CNS), the mTOR pathway regul...The mechanistic target of rapamycin(m TOR) is a serine/threonine kinase that plays a pivotal role in cellular growth, proliferation, survival, and metabolism. In the central nervous system(CNS), the mTOR pathway regulates diverse aspects of neural development and function. Genetic mutations within the m TOR pathway lead to severe neurodevelopmental disorders, collectively known as “mTORopathies”(Crino, 2020). Dysfunctions of m TOR, including both its hyperactivation and hypoactivation, have also been implicated in a wide spectrum of other neurodevelopmental and neurodegenerative conditions, highlighting its importance in CNS health.展开更多
The United Nations Sustainable Development Goal(SDG) 2 aims to achieve Zero Hunger by 2030.However,global hunger and food insecurity have continued to rise at an alarming rate(UN 2023).Subtropical regions are home to ...The United Nations Sustainable Development Goal(SDG) 2 aims to achieve Zero Hunger by 2030.However,global hunger and food insecurity have continued to rise at an alarming rate(UN 2023).Subtropical regions are home to more than 30% of the world's population,predominantly in developing countries where per capita farmland and food supply are only 40% of those in developed nations(FAO 2018).Meeting the Zero Hunger target amid ongoing population growth in these regions requires a substantial increase in agricultural production while minimizing soil degradation and adverse ecological impacts.This challenge is shared by many countries across South Asia,Africa,and Central and South America.展开更多
Innovation and Development Policy,简称“IDP”,中文名:《创新与发展政策(英文)》,是由中国科学院主管,中国科学院科技战略咨询研究院、中国科学学与科技政策研究会共同主办,国内外发行(ISSN:2096-5141,CN:10-1513/D)的英文国际期刊...Innovation and Development Policy,简称“IDP”,中文名:《创新与发展政策(英文)》,是由中国科学院主管,中国科学院科技战略咨询研究院、中国科学学与科技政策研究会共同主办,国内外发行(ISSN:2096-5141,CN:10-1513/D)的英文国际期刊。目前期刊每年出版两期。已发表论文在期刊网站可以自由获取,http://idp-journal.casisd.cn/browse/al/al2019/。展开更多
Standards are the common language that consolidates global consensus and builds the most solid foundation for international partnerships.They are the cornerstone for global sustainable and high-quality development.You...Standards are the common language that consolidates global consensus and builds the most solid foundation for international partnerships.They are the cornerstone for global sustainable and high-quality development.Young students,with their active and vibrant minds,represent the future and hope of standardization.展开更多
China is carving out a distinctive development path which features urban-rural integration.This approach has not only yielded tangible results domestically but also drawn the attention of other countries.
County economies form a distinctive,integrated regional economic structure.Their growth is shaped by specific social and historical conditions and,in turn,influences many aspects of wider economic and social developme...County economies form a distinctive,integrated regional economic structure.Their growth is shaped by specific social and historical conditions and,in turn,influences many aspects of wider economic and social development.In China's modernization process,they have always played a vital role.The 2024 Central Economic Work Conference placed strong emphasis on vigorously developing county economies.展开更多
Objectives:Phosphodiesterase 1A(PDE1A)regulates intracellular cyclic nucleotide signaling and has been implicated in tumor progression,but its clinical relevance and functional role in epithelial ovarian cancer(EOC),p...Objectives:Phosphodiesterase 1A(PDE1A)regulates intracellular cyclic nucleotide signaling and has been implicated in tumor progression,but its clinical relevance and functional role in epithelial ovarian cancer(EOC),particularly in relation to the response to platinum remain unclear.This study aimed to evaluate the clinical significance of PDE1A in EOG and to clarify its functional role in tumor progression and response to platinum-based chemotherapy.Methods:PDE1A mRNA and protein levels were analyzed using public databases,RNA sequencing,and immunohistochemistry.Correlations between PDE1A expression,clinicopathological features,and prognosis were assessed.Functional roles were investigated in ovarian cancer cell lines.Results:PDE1A was significantly overexpressed in EOC tissues compared with that in normal ovarian epithelial tissues.Overexpression correlated with advanced International Federation of Gynecology and Obstetrics(FIGO)stage,poor tumor grade,and reduced response to platinum-based chemotherapy.High PDE1A levels were linked to worse disease-free survival and overall survival,and multivariate analysis confirmed PDE1A as an independent prognostic factor.To elucidate its functional role,we performed in vitro experiments showing that PDE1A knockdown suppressed cell proliferation and colony formation,induced G1 arrest,and downregulatedβ-catenin signaling with reduced cyclin D1 and c-Myc expression.Notably,these inhibitory effects were partially rescued by lithium chloride(LiCl),a Wingless-related integration site(Wnt)/β-catenin activator.Conclusions:In conclusion,our findings identify PDE1A as a Wnt/β-catenin-linked biomarker of tumor progression and platinum resistance in EOC and provide a biological rationale for further investigation of PDE1A-targeted strategies in preclinical models.展开更多
To advance intelligent construction,standards must come first.The Ministry of Housing and Urban-Rural Development has issued the List for Replicable Experience and Practices for Developing Intelligent Construction fou...To advance intelligent construction,standards must come first.The Ministry of Housing and Urban-Rural Development has issued the List for Replicable Experience and Practices for Developing Intelligent Construction four times successively and the Technical Guidelines for Intelligent Construction(Trial).展开更多
1.Background The United Nations(UN)2030 Agenda for Sustainable Development,adopted in 2015,established the Sustainable Development Goals(SDGs)as a comprehensive framework to address global challenges through interconn...1.Background The United Nations(UN)2030 Agenda for Sustainable Development,adopted in 2015,established the Sustainable Development Goals(SDGs)as a comprehensive framework to address global challenges through interconnected social,economic,and environmental targets.展开更多
Dear Editor,We present this case report which discusses a patient who underwent flap amputation after repeated attempts to resolve persistent and severe epithelial ingrowth following laser-assisted in situ keratomileu...Dear Editor,We present this case report which discusses a patient who underwent flap amputation after repeated attempts to resolve persistent and severe epithelial ingrowth following laser-assisted in situ keratomileusis(LASIK)surgery.Epithelial ingrowth is a known complication of LASIK surgery,typically manageable with minimal measures.However,severe cases may necessitate more aggressive interventions,such as flap amputation[1].LASIK is a widely performed refractive surgery with high success rates and excellent visual outcome[2].展开更多
China aims to build on its achievements of the past five years to accelerate rural revitalization during the 15th Five-Year Plan period.IN China,issues relating to agriculture,rural areas,and rural residents have alwa...China aims to build on its achievements of the past five years to accelerate rural revitalization during the 15th Five-Year Plan period.IN China,issues relating to agriculture,rural areas,and rural residents have always been a priority in national development.At its fourth plenary session held in October 2025,the 20th Central Committee of the Communist Party of China(CPC)once again emphasized the need to accelerate agricultural and rural modernization,and advance all-around rural revitalization over the next five years.展开更多
Neurodevelopmental processes represent a finely tuned interplay between genetic and environmental factors,shaping the dynamic landscape of the developing brain.A major component of the developing brain that enables th...Neurodevelopmental processes represent a finely tuned interplay between genetic and environmental factors,shaping the dynamic landscape of the developing brain.A major component of the developing brain that enables this dynamic is the white matter(WM),known to be affected in neurodevelopmental disorders(NDDs)(Rokach et al.,2024).WM formation is mediated by myelination,a multifactorial process driven by neuro-glia interactions dependent on proper neuronal functionality(Simons and Trajkovic,2006).Another key aspect of neurodevelopmental abnormalities involves neuronal dynamics and function,with recent advances significantly enhancing our understanding of both neuronal and glial mitochondrial function(Devine and Kittler,2018;Rojas-Charry et al.,2021).Energy homeostasis in neurons,attributed largely to mitochondrial function,is critical for proper functionality and interactions with oligodendrocytes(OLs),the cells forming myelin in the brain’s WM.We herein discuss the interplay between these processes and speculate on potential dysfunction in NDDs.展开更多
Environmental DNA(eDNA)technology has revolutionized biodiversity monitoring with its non-invasive,sensitive,and cost-efficient approach.This paper systematically reviews eDNA advancements,examining its applications i...Environmental DNA(eDNA)technology has revolutionized biodiversity monitoring with its non-invasive,sensitive,and cost-efficient approach.This paper systematically reviews eDNA advancements,examining its applications in aquatic and terrestrial ecosystems and assessing China’s standardization progress.It delineates four developmental phases from single-species detection to high-throughput sequencing,and highlights China’s contribution to the development of technical standards.While significant progress has been made,challenges persist in quantitative accuracy,methodological consistency,and large-scale implementation.Future efforts should prioritize enhanced standardization,improved quantification techniques,broader applications,and international collaboration to drive innovation in eDNA technology.展开更多
The 20th Communist Party of China(CPC)Central Committee held its fourth plenary session in Beijing on October 20-23.The plenary session's central task was to deliberate on the framework of the country's next n...The 20th Communist Party of China(CPC)Central Committee held its fourth plenary session in Beijing on October 20-23.The plenary session's central task was to deliberate on the framework of the country's next national development roadmap:the 15th Five-Year Plan(2026-2030).Every five-year plan is important but this one arrives at a truly pivotal moment in terms of China's development trajectory and the global environment.展开更多
The development of skeletal muscle are complicated processes involving genes responsible for proper muscle morphology,contractility,cell proliferation,differentiation,interactions,migration,and death.The three-dimensi...The development of skeletal muscle are complicated processes involving genes responsible for proper muscle morphology,contractility,cell proliferation,differentiation,interactions,migration,and death.The three-dimensional chromatin architecture of skeletal muscle development has not been studied intensively although dynamic transcriptional regulation during differentiation of muscle cells is one of the most deeply studied processes.The RNA-seq was used to analyze the transcriptome pattern during chicken muscle development across 12 stages.Hi-C was used to build chromatin architectures during four representative stages.Ch IP-seq was conducted to identify enhancers and promoters in these four stages,which are occupied by histone H3K27ac and H3K4me3 peaks.Results show that large-scale genome architecture changes are mostly unidirectional,and coupled by complex on/off dynamic patterns of gene expression.Specifically,we observed 258.30 Mb of the genome undergoing A/B compartment switching.Notable alterations(316.57 Mb)of interaction frequencies within TADs were observed.Substantial aging-associated genes exhibited ascending connectivity with the compartment transition from repressive to active status during muscle development.Some muscle-related gene promoters that interacted with active enhancers during development,and some myopathy/aging-associated genes that were activated in aging muscle were founded.These results provide key insights into skeletal muscle development in vivo,and offer a valuable resource that allows in-depth functional characterization of candidate genes.展开更多
AIM:To investigate whether vaccinia-related kinase 1(VRK1)mediates transforming growth factor-beta2(TGF-β2)-caused epithelial-mesenchymal transition(EMT)and inflammatory responses in retinal pigment epithelial(RPE)ce...AIM:To investigate whether vaccinia-related kinase 1(VRK1)mediates transforming growth factor-beta2(TGF-β2)-caused epithelial-mesenchymal transition(EMT)and inflammatory responses in retinal pigment epithelial(RPE)cells through regulating snail family transcriptional repressor 1(SNAI1),and to validate its role in a proliferative vitreoretinopathy(PVR)mouse model.METHODS:Human RPE cell line ARPE-19 cells were treated with TGF-β2 to construct an EMT model.Western blot detected VRK1 level.The effects of VRK1 on SNAI1 expression and biological behavior of ARPE-19 cells were detected by immunofluorescence,ELISA,Transwell,and scratch assay,and the interaction between VRK1 and SNAI1 was confirmed through immunoprecipitation.A PVR mouse model was constructed,and the effects of VRK1 or/and SNAI1 on retinal damage were assessed by pathologic staining.Inflammatory factors and EMT-related proteins were assessed with ELISA and Western blot.RESULTS:VRK1 was upregulated in ARPE-19 cells after TGF-β2 treatment.Overexpression of VRK1 increased cell viability,promoted cell migration and EMT,and the levels of inflammatory factors.Silencing of VRK1 reversed the above indexes.There was a direct interaction between VRK1 and SNAI1,and overexpresssion SNAI1 weakened the impacts of silencing of VRK1.In PVR mice,silencing of VRK1 ameliorated retinal structural damage,decreased proinflammatory factor levels,and suppressed SNAI1 and mesenchymal marker expression.SNAI1 overexpression antagonized the protective effects of silencing VRK1 and exacerbated EMT and inflammatory responses.CONCLUSION:VRK1 plays a key role in retinal structural and inflammatory damage in PVR mice by regulating SNAI1 and mediating TGF-β2-caused EMT and inflammatory responses in RPE cells.展开更多
Against the backdrop of China s accelerated green development and its pursuit of the"dual carbon"goals,green finance,as a crucial instrument for the rational allocation of resources,plays a significant role ...Against the backdrop of China s accelerated green development and its pursuit of the"dual carbon"goals,green finance,as a crucial instrument for the rational allocation of resources,plays a significant role in promoting agricultural green transformation.Taking Henan Province as a case study,this research employs panel data from 18 prefecture-level cities spanning 2014 to 2023,utilizing dynamic panel models and mediating effect models for empirical analysis.The findings reveal that green finance indirectly fosters agricultural green development by driving agricultural technological progress.This promoting effect is significant,with the mediating effect accounting for 3.67%.Based on these results,policy recommendations are proposed:expanding the coverage of green finance and constructing a diversified product system;comprehensively promoting agricultural technological innovation and mechanization development;and coordinating and integrating green financial resources to facilitate agricultural green transformation tailored to local conditions.展开更多
The International Standardization Youth Star Competition is held for three consecutive years.We have witnessed continuous improvement in the competition each year,which is a result of our collective efforts.
The dawn in Gyirong arrives softly,as golden light spills across snow-capped peaks and filters down into the deep valleys.In Jifu Village,nestled along the Gyirong Zangbo River,the morning air is always moist,carrying...The dawn in Gyirong arrives softly,as golden light spills across snow-capped peaks and filters down into the deep valleys.In Jifu Village,nestled along the Gyirong Zangbo River,the morning air is always moist,carrying the fragrance of water and soil.For centuries,this Chinese border village near Nepal has been a crossroad of cultures.It was here,in 1994,that a girl named Langsa was bom as the eldest of five siblings.展开更多
N6-methyladenosine(m^(6)A)modification,the most abundant internal modification in messenger RNA(mRNA)and long non-coding RNA(lncRNA),has emerged as a critical epitranscriptomic regulatory mechanism in eukaryotes.While...N6-methyladenosine(m^(6)A)modification,the most abundant internal modification in messenger RNA(mRNA)and long non-coding RNA(lncRNA),has emerged as a critical epitranscriptomic regulatory mechanism in eukaryotes.While the importance of m^(6)A modification in various biological processes has been recognized,a comprehensive understanding of its diverse roles in plant biology and agricultural applications remains fragmented.This review analyzes recent advances inm^(6)A modification's biological functions in plants.m^(6)A modification plays crucial roles in multiple aspects of plant life,including seed germination,organ development,and reproductive structure formation.Furthermore,m^(6)A has been found to significantly influence plant responses to environmental stresses,including salt,drought,temperature,and heavy metal exposure.We also uncover m^(6)A involvement in important agricultural traits.This review provides insights into the mechanistic understanding of m^(6)A modification in plants and highlights its applications in agricultural improvement,offering a foundation for future research in crop enhancement and stress resistance.展开更多
基金supported by grants from Simons Foundation (SFARI 479754),CIHR (PJT-180565)the Scottish Rite Charitable Foundation of Canada (to YL)funding from the Canada Research Chairs program。
文摘The mechanistic target of rapamycin(m TOR) is a serine/threonine kinase that plays a pivotal role in cellular growth, proliferation, survival, and metabolism. In the central nervous system(CNS), the mTOR pathway regulates diverse aspects of neural development and function. Genetic mutations within the m TOR pathway lead to severe neurodevelopmental disorders, collectively known as “mTORopathies”(Crino, 2020). Dysfunctions of m TOR, including both its hyperactivation and hypoactivation, have also been implicated in a wide spectrum of other neurodevelopmental and neurodegenerative conditions, highlighting its importance in CNS health.
文摘The United Nations Sustainable Development Goal(SDG) 2 aims to achieve Zero Hunger by 2030.However,global hunger and food insecurity have continued to rise at an alarming rate(UN 2023).Subtropical regions are home to more than 30% of the world's population,predominantly in developing countries where per capita farmland and food supply are only 40% of those in developed nations(FAO 2018).Meeting the Zero Hunger target amid ongoing population growth in these regions requires a substantial increase in agricultural production while minimizing soil degradation and adverse ecological impacts.This challenge is shared by many countries across South Asia,Africa,and Central and South America.
文摘Innovation and Development Policy,简称“IDP”,中文名:《创新与发展政策(英文)》,是由中国科学院主管,中国科学院科技战略咨询研究院、中国科学学与科技政策研究会共同主办,国内外发行(ISSN:2096-5141,CN:10-1513/D)的英文国际期刊。目前期刊每年出版两期。已发表论文在期刊网站可以自由获取,http://idp-journal.casisd.cn/browse/al/al2019/。
文摘Standards are the common language that consolidates global consensus and builds the most solid foundation for international partnerships.They are the cornerstone for global sustainable and high-quality development.Young students,with their active and vibrant minds,represent the future and hope of standardization.
文摘China is carving out a distinctive development path which features urban-rural integration.This approach has not only yielded tangible results domestically but also drawn the attention of other countries.
文摘County economies form a distinctive,integrated regional economic structure.Their growth is shaped by specific social and historical conditions and,in turn,influences many aspects of wider economic and social development.In China's modernization process,they have always played a vital role.The 2024 Central Economic Work Conference placed strong emphasis on vigorously developing county economies.
基金supported by the National Research Foundation of Korea(NRF)grant,funded by the Korean government(MIST),Jae-Hoon Kim(NRF-2020R1A2C2004782)Hanbyoul Cho(NRF-RS-2025-00522191)of Funderssupported by the Bio&Medical Technology Development Program of the National Research Foundation(NRF),funded by the Korean Government(MSIT),Jae-Hoon Kim of Funder(NRF-2017M3A9B 8069610).
文摘Objectives:Phosphodiesterase 1A(PDE1A)regulates intracellular cyclic nucleotide signaling and has been implicated in tumor progression,but its clinical relevance and functional role in epithelial ovarian cancer(EOC),particularly in relation to the response to platinum remain unclear.This study aimed to evaluate the clinical significance of PDE1A in EOG and to clarify its functional role in tumor progression and response to platinum-based chemotherapy.Methods:PDE1A mRNA and protein levels were analyzed using public databases,RNA sequencing,and immunohistochemistry.Correlations between PDE1A expression,clinicopathological features,and prognosis were assessed.Functional roles were investigated in ovarian cancer cell lines.Results:PDE1A was significantly overexpressed in EOC tissues compared with that in normal ovarian epithelial tissues.Overexpression correlated with advanced International Federation of Gynecology and Obstetrics(FIGO)stage,poor tumor grade,and reduced response to platinum-based chemotherapy.High PDE1A levels were linked to worse disease-free survival and overall survival,and multivariate analysis confirmed PDE1A as an independent prognostic factor.To elucidate its functional role,we performed in vitro experiments showing that PDE1A knockdown suppressed cell proliferation and colony formation,induced G1 arrest,and downregulatedβ-catenin signaling with reduced cyclin D1 and c-Myc expression.Notably,these inhibitory effects were partially rescued by lithium chloride(LiCl),a Wingless-related integration site(Wnt)/β-catenin activator.Conclusions:In conclusion,our findings identify PDE1A as a Wnt/β-catenin-linked biomarker of tumor progression and platinum resistance in EOC and provide a biological rationale for further investigation of PDE1A-targeted strategies in preclinical models.
文摘To advance intelligent construction,standards must come first.The Ministry of Housing and Urban-Rural Development has issued the List for Replicable Experience and Practices for Developing Intelligent Construction four times successively and the Technical Guidelines for Intelligent Construction(Trial).
文摘1.Background The United Nations(UN)2030 Agenda for Sustainable Development,adopted in 2015,established the Sustainable Development Goals(SDGs)as a comprehensive framework to address global challenges through interconnected social,economic,and environmental targets.
文摘Dear Editor,We present this case report which discusses a patient who underwent flap amputation after repeated attempts to resolve persistent and severe epithelial ingrowth following laser-assisted in situ keratomileusis(LASIK)surgery.Epithelial ingrowth is a known complication of LASIK surgery,typically manageable with minimal measures.However,severe cases may necessitate more aggressive interventions,such as flap amputation[1].LASIK is a widely performed refractive surgery with high success rates and excellent visual outcome[2].
文摘China aims to build on its achievements of the past five years to accelerate rural revitalization during the 15th Five-Year Plan period.IN China,issues relating to agriculture,rural areas,and rural residents have always been a priority in national development.At its fourth plenary session held in October 2025,the 20th Central Committee of the Communist Party of China(CPC)once again emphasized the need to accelerate agricultural and rural modernization,and advance all-around rural revitalization over the next five years.
文摘Neurodevelopmental processes represent a finely tuned interplay between genetic and environmental factors,shaping the dynamic landscape of the developing brain.A major component of the developing brain that enables this dynamic is the white matter(WM),known to be affected in neurodevelopmental disorders(NDDs)(Rokach et al.,2024).WM formation is mediated by myelination,a multifactorial process driven by neuro-glia interactions dependent on proper neuronal functionality(Simons and Trajkovic,2006).Another key aspect of neurodevelopmental abnormalities involves neuronal dynamics and function,with recent advances significantly enhancing our understanding of both neuronal and glial mitochondrial function(Devine and Kittler,2018;Rojas-Charry et al.,2021).Energy homeostasis in neurons,attributed largely to mitochondrial function,is critical for proper functionality and interactions with oligodendrocytes(OLs),the cells forming myelin in the brain’s WM.We herein discuss the interplay between these processes and speculate on potential dysfunction in NDDs.
基金supported by the National Natural Science Foundation of China(Grant No.32160172)the Key Science-Technology Project of Inner Mongolia(2023KYPT0010)+1 种基金the Natural Science Foundation of Inner Mongolia Autonomous Region of China(Grant No.2025QN03006)the 2023 Inner Mongolia Public Institution High-Level Talent Introduction Scientific Research Support Project.
文摘Environmental DNA(eDNA)technology has revolutionized biodiversity monitoring with its non-invasive,sensitive,and cost-efficient approach.This paper systematically reviews eDNA advancements,examining its applications in aquatic and terrestrial ecosystems and assessing China’s standardization progress.It delineates four developmental phases from single-species detection to high-throughput sequencing,and highlights China’s contribution to the development of technical standards.While significant progress has been made,challenges persist in quantitative accuracy,methodological consistency,and large-scale implementation.Future efforts should prioritize enhanced standardization,improved quantification techniques,broader applications,and international collaboration to drive innovation in eDNA technology.
文摘The 20th Communist Party of China(CPC)Central Committee held its fourth plenary session in Beijing on October 20-23.The plenary session's central task was to deliberate on the framework of the country's next national development roadmap:the 15th Five-Year Plan(2026-2030).Every five-year plan is important but this one arrives at a truly pivotal moment in terms of China's development trajectory and the global environment.
基金supported by the National Key R&D Program of China(2023YFD1300040 and 2022YFF1000100)the Sichuan Science and Technology Program,China(2022NSFSC0132,2021YFYZ0009 and 2022JDJQ0054)the National Natural Science Foundation of China(32225046)。
文摘The development of skeletal muscle are complicated processes involving genes responsible for proper muscle morphology,contractility,cell proliferation,differentiation,interactions,migration,and death.The three-dimensional chromatin architecture of skeletal muscle development has not been studied intensively although dynamic transcriptional regulation during differentiation of muscle cells is one of the most deeply studied processes.The RNA-seq was used to analyze the transcriptome pattern during chicken muscle development across 12 stages.Hi-C was used to build chromatin architectures during four representative stages.Ch IP-seq was conducted to identify enhancers and promoters in these four stages,which are occupied by histone H3K27ac and H3K4me3 peaks.Results show that large-scale genome architecture changes are mostly unidirectional,and coupled by complex on/off dynamic patterns of gene expression.Specifically,we observed 258.30 Mb of the genome undergoing A/B compartment switching.Notable alterations(316.57 Mb)of interaction frequencies within TADs were observed.Substantial aging-associated genes exhibited ascending connectivity with the compartment transition from repressive to active status during muscle development.Some muscle-related gene promoters that interacted with active enhancers during development,and some myopathy/aging-associated genes that were activated in aging muscle were founded.These results provide key insights into skeletal muscle development in vivo,and offer a valuable resource that allows in-depth functional characterization of candidate genes.
文摘AIM:To investigate whether vaccinia-related kinase 1(VRK1)mediates transforming growth factor-beta2(TGF-β2)-caused epithelial-mesenchymal transition(EMT)and inflammatory responses in retinal pigment epithelial(RPE)cells through regulating snail family transcriptional repressor 1(SNAI1),and to validate its role in a proliferative vitreoretinopathy(PVR)mouse model.METHODS:Human RPE cell line ARPE-19 cells were treated with TGF-β2 to construct an EMT model.Western blot detected VRK1 level.The effects of VRK1 on SNAI1 expression and biological behavior of ARPE-19 cells were detected by immunofluorescence,ELISA,Transwell,and scratch assay,and the interaction between VRK1 and SNAI1 was confirmed through immunoprecipitation.A PVR mouse model was constructed,and the effects of VRK1 or/and SNAI1 on retinal damage were assessed by pathologic staining.Inflammatory factors and EMT-related proteins were assessed with ELISA and Western blot.RESULTS:VRK1 was upregulated in ARPE-19 cells after TGF-β2 treatment.Overexpression of VRK1 increased cell viability,promoted cell migration and EMT,and the levels of inflammatory factors.Silencing of VRK1 reversed the above indexes.There was a direct interaction between VRK1 and SNAI1,and overexpresssion SNAI1 weakened the impacts of silencing of VRK1.In PVR mice,silencing of VRK1 ameliorated retinal structural damage,decreased proinflammatory factor levels,and suppressed SNAI1 and mesenchymal marker expression.SNAI1 overexpression antagonized the protective effects of silencing VRK1 and exacerbated EMT and inflammatory responses.CONCLUSION:VRK1 plays a key role in retinal structural and inflammatory damage in PVR mice by regulating SNAI1 and mediating TGF-β2-caused EMT and inflammatory responses in RPE cells.
基金Supported by Zhejiang Provincial Philosophy and Social Sciences Planning Project(23FNSQ58YB).
文摘Against the backdrop of China s accelerated green development and its pursuit of the"dual carbon"goals,green finance,as a crucial instrument for the rational allocation of resources,plays a significant role in promoting agricultural green transformation.Taking Henan Province as a case study,this research employs panel data from 18 prefecture-level cities spanning 2014 to 2023,utilizing dynamic panel models and mediating effect models for empirical analysis.The findings reveal that green finance indirectly fosters agricultural green development by driving agricultural technological progress.This promoting effect is significant,with the mediating effect accounting for 3.67%.Based on these results,policy recommendations are proposed:expanding the coverage of green finance and constructing a diversified product system;comprehensively promoting agricultural technological innovation and mechanization development;and coordinating and integrating green financial resources to facilitate agricultural green transformation tailored to local conditions.
文摘The International Standardization Youth Star Competition is held for three consecutive years.We have witnessed continuous improvement in the competition each year,which is a result of our collective efforts.
文摘The dawn in Gyirong arrives softly,as golden light spills across snow-capped peaks and filters down into the deep valleys.In Jifu Village,nestled along the Gyirong Zangbo River,the morning air is always moist,carrying the fragrance of water and soil.For centuries,this Chinese border village near Nepal has been a crossroad of cultures.It was here,in 1994,that a girl named Langsa was bom as the eldest of five siblings.
基金supported by the National Nature Science Foundation of China(Grant No.31660568)Guangxi Science and Technology major project(Grant No.GuikeAA22068088)+2 种基金start-up funding for introduced talents in Guangxi University,the Guangxi Colleges and Universities Young and Middle-aged Teachers'Basic Scientific Research Ability Improvement Project(Grant No.2024KY0010)Guangxi Graduate Education Innovation Program(Grant No.YCSW2024093)the Guangxi University Student Innovation and Entrepreneurship Training Program Funding Project(Grant Nos.202310593704,202310593714,202410953044S).
文摘N6-methyladenosine(m^(6)A)modification,the most abundant internal modification in messenger RNA(mRNA)and long non-coding RNA(lncRNA),has emerged as a critical epitranscriptomic regulatory mechanism in eukaryotes.While the importance of m^(6)A modification in various biological processes has been recognized,a comprehensive understanding of its diverse roles in plant biology and agricultural applications remains fragmented.This review analyzes recent advances inm^(6)A modification's biological functions in plants.m^(6)A modification plays crucial roles in multiple aspects of plant life,including seed germination,organ development,and reproductive structure formation.Furthermore,m^(6)A has been found to significantly influence plant responses to environmental stresses,including salt,drought,temperature,and heavy metal exposure.We also uncover m^(6)A involvement in important agricultural traits.This review provides insights into the mechanistic understanding of m^(6)A modification in plants and highlights its applications in agricultural improvement,offering a foundation for future research in crop enhancement and stress resistance.
