A modified separation method has been developed for determinating polychlorinated biphenyl congeners in environmental samples. Direct treatment of extract with concentrated HzSO4 was employed in the first step for rem...A modified separation method has been developed for determinating polychlorinated biphenyl congeners in environmental samples. Direct treatment of extract with concentrated HzSO4 was employed in the first step for removal of lipids and other interfering substances, then a joint column of alumina-silica gel(Ag+) was applied to separate PCBs fraction from HCH, DDT and its analogs. After this separation, the PCBs fraction was analyzed by capillary gas chromatography with ECD detector and confirmed by GC/MS. The recoveries of individual congeners in Aroclor 1254 through the separation are about 79%-84%. The method is very efficient and useful for determination of trace amount of PCB congeners in environmental samples.展开更多
The lactose-fermenter Enterobacteriaceae are the most frequent cause of clinical infection in our country. The objective of this study was to isolate and identify the most common lactose-fermenter Enterobacteriaceae f...The lactose-fermenter Enterobacteriaceae are the most frequent cause of clinical infection in our country. The objective of this study was to isolate and identify the most common lactose-fermenter Enterobacteriaceae from clinical samples, including urine, blood, wounds, and sputum, obtained from the local hospital and from environmental samples from a chicken farm, agriculture soil, and water from the Tigris River in Baghdad City. The study also aimed at establishing the antibiotic resistance patterns of the isolated bacteria. A total of 155 bacterial isolates were identified from 10 genera according to the Vitek 2 system. The most common bacterial isolates from the clinical and environmental samples were Escherichia coli and Klebsiella pneumoniae, respectively. The antibiotic resistance patterns showed that all clinical and environmental isolates were multidrug resistant to β-lactam (except carbapenems) drug and aminoglycosides and more sensitive to carbapenems.展开更多
This work presents a multi-residue analytical method based on solid phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) with diode array (DAD) detection for the simultaneous determination...This work presents a multi-residue analytical method based on solid phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) with diode array (DAD) detection for the simultaneous determination of a group of pharmaceutical products that include ten antidepressants and three anticanceri- genic in environmental samples (water and soil). Baseline separation of the studied compounds was obtained on an ultrabase C18 (4.6 mm i.d. × 150 mm, 5 μm particle) column using acetonitrile:phosphate buffer pH 2.5 (35:65 v/v) as mobile phase with a flow rate of 1.5 mL/min. Different aspects including linearity, accuracy, precision and detection and quantification limits were examined in order to validate the proposed method. Detection limits between 1 and 50 ng/mL were obtained for all the target compounds. This method was ap- plied to the analysis of environmental samples as waters and soils of different precedence. Prior, the HPLC determination the samples were purified and enriched using SPE or liquid-liquid extraction (LLE) of the tar- get compounds.展开更多
A closed-vessel microwave digestion method is described for the rapid dissolution of environmental samples such as foods, soils and sediments. Depending on the sample type, 0.1-0.2 g sample was decomposed with HNO3/H2...A closed-vessel microwave digestion method is described for the rapid dissolution of environmental samples such as foods, soils and sediments. Depending on the sample type, 0.1-0.2 g sample was decomposed with HNO3/H2O2 or HNO3 / H2O2/HF acid mixture in a PTFE digestion vessel by using microwave heating for 2-3 min at 500W of microwave power. The solution, or to which 0.5 g of boric acid was added, was diluted to 25-50 ml and directly determined by sequential ICP-AES. The accuracy of the procedure was validated by the analysis of six standard reference materials for 10 elements. Ail results were in a good agreements with the certified values.展开更多
Surface-enhanced Raman scattering(SERS)has been widely used in various fields due to high sensitivity,but it is hindered by its low repeatability.Here,we explored a highly reproducible free-standing liquid-state platf...Surface-enhanced Raman scattering(SERS)has been widely used in various fields due to high sensitivity,but it is hindered by its low repeatability.Here,we explored a highly reproducible free-standing liquid-state platform(FSM)for monitoring norfloxacin(NOR)in the environmental field.After optimizing the types of nanoparticles(NPs)and pH value in NOR solution,the relative standard deviation(RSD)of FSM SERS analysis can be stabilized below 10%(n≥8),and the R^(2)of the calibration curve in the range of 20μg/L-100μg/L was 0.995.In addition,the results showed that the limit of detection(LOD)(3σ/k)of NOR was 1.3μg/L.Moreover,the NOR in the spiked samples was successfully quantified by FSM,and the recovery rate exceeded 95%.The proposed method has the potential for rapid analysis of drug residues in environmental samples.展开更多
Organophosphate nerve agents(OPNAs)are lethal chemical weapons(CWs)which hydrolyze rapidly to alkyl phosphate compounds in the environment.The identification of alkyl phosphate compounds in the environment is critical...Organophosphate nerve agents(OPNAs)are lethal chemical weapons(CWs)which hydrolyze rapidly to alkyl phosphate compounds in the environment.The identification of alkyl phosphate compounds in the environment is critical for the verification of OPNAs.Currently,due to the complex sample preparation and long detection process for organophosphate nerve agent hydrolysis products(OPNAHPs),their high-throughput determination is difficult.To simplify sample preparation and detection of OPNAHPs from environmental samples,a quick,easy,cheap,effective,rugged and safe(Qu ECh ERS)method for the simultaneous purification of the five OPNAHPs in environmental samples combined with detection through direct analysis in real-time ionization source coupled with tandem mass spectrometer(DART-MS/MS)was developed.An internal standard method was applied for the quantification.The method exhibited a good linearity(R^(2)>0.991)in the range of 1.00 ng/m L-4.00μg/m L.The limits of detection and limits of quantification were 1.00-7.00 ng/m L and 10.0-20.0 ng/m L,respectively.The recoveries of OPNAHPs in environmental samples were between 65.3%and 107%,with relative standard deviations ranged from 1.6%to 19%.In addition,the sample preparation and analysis process for OPNAHPs could be completed in less than 10 min.The results demonstrated that the modified Qu ECh ERS method could quickly and efficiently remove the matrix of environmental samples.Combined with DART-MS/MS,an accurate and high-throughput detection method of OPNAHPs in environmental samples was provided.The developed method has great potential in the fields of early warning,clinical treatment and environmental decontamination.展开更多
Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not mee...Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry(EDS) can be used to simultaneously detect multiple elements in a sample, including sulfur, with minimal sample treatment, this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples, with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells, while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium, which are sulfur oxidizers. Thus, EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells, within a short time(within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.展开更多
Trace of Pb(Ⅱ) has been on-line separated and enriched from environmental samples and wastewater by using the self-made alizarin violet functionalized silica gel micro-column coupling with a sequential injection samp...Trace of Pb(Ⅱ) has been on-line separated and enriched from environmental samples and wastewater by using the self-made alizarin violet functionalized silica gel micro-column coupling with a sequential injection sampling technology. The determination is based on the color reaction of Pb(Ⅱ) with iodide and crystal violet to form an ionic association complex in the presence of polyvinyl alcohol and hydrochloric acid. The use of the microcolumn can prevent the interference of most familiar metal ions, and therefore improve the selectivity and sensitivity of this analytical technique. The proposed method was used for the determination of Pb(Ⅱ) in environmental samples and wastewater. No statistically significant difference was observed between the results determined by the present method and atomic absorption spectrometry.展开更多
Lymnaeid snails are key intermediate hosts for the development and survival of Fasciola spp.,the causative agent of Fascioliasis which are economically important parasites infecting humans and livestock globally.The c...Lymnaeid snails are key intermediate hosts for the development and survival of Fasciola spp.,the causative agent of Fascioliasis which are economically important parasites infecting humans and livestock globally.The current control method for treating Fascioliasis is heavily reliant on anthelmintic drugs,particularly Triclabendazole(TCBZ)which has resulted in drug-resistant parasites and poses significant risk as there are no long-term efficacious alternatives available.Sustainable control measures at the farm level could include both parasite and snail control will play an important role in Fasciola spp.control and reduce the reliance on anthelmintic drugs.Implementation of such sustainable control measures requires effective identification of snails on the property however Lymnaeid snails are small and difficult to physically locate.Snail identification using an environmental DNA approach is a recent approach in which physically locating snails are not required.Austropeplea tomentosa,is the primary intermediate snail host for F.hepatica transmission in South-East Australia and we present an in-field loop-mediated isothermal amplification and water filtering method for the detection of A.tomentosa eDNA from water samples to improve current surveillance methods.This methodology is highly sensitive with a detection limit of 5×10^(−6)ng/μL,detected in<20 minutes,with cumulative sample preparation and amplification time under 1 hour.This proposed workflow could assist in monitoring areas to determine the risk of Fascioliasis infection and implement strategies to manage snail populations to ultimately reduce the risk of infection for humans and livestock.展开更多
Based on poly(vinyl chloride) membranes, a novel miniaturized screen-printed all-solid-state copper(II)-selective electrode has been developed for applications in environmental monitoring. Performance and applicabilit...Based on poly(vinyl chloride) membranes, a novel miniaturized screen-printed all-solid-state copper(II)-selective electrode has been developed for applications in environmental monitoring. Performance and applicability of the ion-selective electrode (ISE) have been proved by potentiometric investigations. Conducting polymers were used as intermediate layers and as solid contacts between the ion-selective membrane and the graphite transducer. The ion-complexing reagent 2-mercapto-benzoxazole was incorporated into poly(vinyl chloride) membranes. In the concentration range 10<sup>-6</sup> - 10<sup>-2</sup> mol/L, the ISE exhibited a linear Nernstian potential response to copper(II) with an average slope value of 28 mV/decade. The detection limit was 3 × 10<sup>-7</sup> mol/L. The electrode exhibits a short response time (<10 s) and can be used in the range of pH = 3 - 7. Selectivity coefficents against certain interfering ions are investigated. The life time of the electrode under laboratory conditions was approximately 12-month. The electrode was applied in the investigation of different aqueous environmental samples and the electrode characteristics were described. The copper(II) ASS electrode has also successfully been used in potentiometric, complexometric titrations with ethylenediaminetetraacetic acid.展开更多
Sequencing of environmental samples has great potential for biodiversity research,but its application is limited by the lack of reliable DNA barcode databases for species identifications.Such a database has been creat...Sequencing of environmental samples has great potential for biodiversity research,but its application is limited by the lack of reliable DNA barcode databases for species identifications.Such a database has been created for epiphytic lichens of Europe,allowing us to compare the results of environmental sequencing with standard taxonomic surveys.The species undetected by taxonomic surveys(what we term the ghost component)amount to about half of the species actually present in hectare plots of Central European forests.Some of these,which currently occur only as diaspores or weakly developed thalli,are likely to be favoured in the course of global change.The ghost component usually represents a larger fraction in managed forests than in old-growth unmanaged forests.The total species composition of different plots is much more similar than suggested by taxonomic surveys alone.On a regional scale,this supports the well-known statement that“everything is everywhere,but,the environment selects”.展开更多
Healthcare workers(HCWs)are at high risk of occupational exposure to the new pandemic human coronavirus,SARSCoV-2,and are a source of nosocomial transmission in airborne infectious isolation rooms(AIIRs).Here,we perfo...Healthcare workers(HCWs)are at high risk of occupational exposure to the new pandemic human coronavirus,SARSCoV-2,and are a source of nosocomial transmission in airborne infectious isolation rooms(AIIRs).Here,we performed comprehensive environmental contamination surveillance to evaluate the risk of viral transmission in AIIRs with 115 rooms in three buildings at the Shanghai Public Health Clinical Center,Shanghai,during the treatment of 334 patients infected with SARS-CoV-2.The results showed that the risk of airborne transmission of SARS-CoV-2 in AIIRs was low(1.62%,25/1544)due to the directional airflow and strong environmental hygiene procedures.However,we detected viral RNA on the surface of foot-operated openers and bathroom sinks in AIIRs(viral load:55.00–3154.50 copies/mL).This might be a source of contamination to connecting corridors and object surfaces through the footwear and gloves used by HCWs.The risk of infection was eliminated by the use of disposable footwear covers and the application of more effective environmental and personal hygiene measures.With the help of effective infection control procedures,none of 290 HCWs was infected when working in the AIIRs at this hospital.This study has provided information pertinent for infection control in AIIRs during the treatment of COVID-19 patients.展开更多
Helicobacter pylori are ubiquitous Gram-negative bacteria with a high estimated level of infection in the world populations, but a majority of the infected persons are asymptomatic. This pathogen has been classified b...Helicobacter pylori are ubiquitous Gram-negative bacteria with a high estimated level of infection in the world populations, but a majority of the infected persons are asymptomatic. This pathogen has been classified by the World Health Organization as a class I carcinogen and recognized as the causal agent of most peptic ulcers and chronic gastritis that might lead to stomach cancer. Although not all the transmission pathways of these bacteria into humans have been propedy identified, enough data have suggested that the oral-oral or fecal-oral ones are the main infection routes. Helicobacterpylori have been detected in non-treated water and in drinking water, which suggested that water might be an important infection source. As childhood is the critical period of infection, the aim of the present work was to examine the presence of Helicobacterpylori in soil samples from public playing areas of Spanish parks.展开更多
Trace analysis of volatile organic compounds(VOCs) during wildfires is imperative for environmental and health risk assessment. The use of gas sampling devices mounted on unmanned aerial vehicles(UAVs) to chemically s...Trace analysis of volatile organic compounds(VOCs) during wildfires is imperative for environmental and health risk assessment. The use of gas sampling devices mounted on unmanned aerial vehicles(UAVs) to chemically sample air during wildfires is of great interest because these devices move freely about their environment, allowing for more representative air samples and the ability to sample areas dangerous or unreachable by humans. This work presents chemical data from air samples obtained in Davis, CA during the most destructive wildfire in California's history-the 2018 Camp Fire – as well as the deployment of our sampling device during a controlled experimental fire while fixed to a UAV. The sampling mechanism was an in-house manufactured micro-gas preconcentrator(μPC) embedded onto a compact battery-operated sampler that was returned to the laboratory for chemical analysis. Compounds commonly observed in wildfires were detected during the Camp Fire using gas chromatography mass spectrometry(GC–MS), including BTEX(benzene, toluene, ethylbenzene, m + p-xylene, and o-xylene), benzaldehyde, 1,4-dichlorobenzene, naphthalene, 1,2,3-trimethylbenzene and 1-ethyl-3-methylbenzene. Concentrations of BTEX were calculated and we observed that benzene and toluene were highest with average concentrations of 4.7 and 15.1 μg/m^(3), respectively. Numerous fire-related compounds including BTEX and aldehydes such as octanal and nonanal were detected upon experimental fire ignition, even at a much smaller sampling time compared to samples taken during the Camp Fire. Analysis of the air samples taken both stationary during the Camp Fire and mobile during an experimental fire show the successful operation of our sampler in a fire environment.展开更多
Antibiotics,as widely used antibacterial drug,exist in various environmental media.Antibiotic residues can affect biological metabolism and lead to bacterial resistance and the formation of antibiotic-resistance genes...Antibiotics,as widely used antibacterial drug,exist in various environmental media.Antibiotic residues can affect biological metabolism and lead to bacterial resistance and the formation of antibiotic-resistance genes,posing a threat to human health and ecological safety.Establishing efficient detection methods for antibiotics and antibiotic-resistance genes has great environmental significance.Fluorescence detection methods,due to their fast response,high sensitivity and specificity,and low-cost,are widely used in chemical and biological sensing.This review first summarizes the pre-treatment methods for different types of environmental samples,and then focuses on the recent advances of fluorescence methods for the detection of antibiotics and antibiotic-resistance genes.Finally,main challenges and future research directions of fluorescence methods for antibiotic and antibiotic-resistance genes detection are discussed.This review highlights the promising prospect of fluorescence methods in-situ detection and monitoring of antibiotics and antibiotic-resistance genes,and provides guidance for the construction of overall risk assessment system of environmental media.展开更多
Introduction:The establishment of a highthroughput quantification approach for waterborne pathogenic protozoa and helminths is crucial for rapid screening and health risk assessment.Methods:We developed a high-through...Introduction:The establishment of a highthroughput quantification approach for waterborne pathogenic protozoa and helminths is crucial for rapid screening and health risk assessment.Methods:We developed a high-throughput quantitative polymerase chain reaction(HT-qPCR)assay targeting 19 waterborne protozoa and 3 waterborne helminths and validated its sensitivity,specificity,and repeatability.The assay was then applied to test various environmental media samples.Results:The HT-qPCR assay's limit of detection(LOD)was 5×10^(2) copies/μL DNA,and its specificity was confirmed using Giardia and Cryptosporidium standards.Repeatability,assessed through intra-and inter-group experiments,yielded a coefficient of variation(CV)of 1.0%–4.6%and 1.2%–6.4%at concentrations of 1×10^(5) and 1×10^(4) copies/μL,respectively.The R^(2) values of the 22 standard curves ranged from 0.983 to 0.998,with amplification efficiencies between 80%and 107%.In drinking water sources,sludge from municipal wastewater treatment plants(MWTPs),and livestock manure samples,17 of 22 targets were detected,with Acanthamoeba genus(50.0%),Acanthamoeba castellanii(11.8%),and Enterocytozoon bieneusi(11.8%)showing high prevalence.Cryptosporidium spp.,Enterocytozoon bieneusi,and Cyclospora cayetanensis were simultaneously found in all three sample types.Discussion:This study presents a useful tool for the rapid detection of waterborne protozoa and helminths in complex environmental microbiomes,providing scientific data for monitoring cross-media transmission and controlling microbial risk from a One Health perspective.展开更多
Objective To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy （GFAAS） and inductively coupled plasma atomic emission spectroscopy （ICPAES）. Methods An indirect co...Objective To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy （GFAAS） and inductively coupled plasma atomic emission spectroscopy （ICPAES）. Methods An indirect competitive enzyme-linked immunosorbent assay （IC-ELISA） was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated. Results IC-ELISA showed an IC50 of 45.6 μg/L with a detection limit of 1.95 μg/L for cadmium, and showed a mean recovery ranging 97.67%-107.08%. The coefficient of variations for intra- and interassay was 3.41%-6.61% and 4.70%-9.21%, respectively. The correlation coefficient between IC-ELISA and GFAAS was 0.998. Conclusion IC-ELISA can detect and quantify cadmium residue in environmental or food samples.展开更多
A signal-amplified mercury sensing biosensor with desired sensitivity was developed through firstly using the GFP mutant with fluorescence increasing response towards Hg^2+ as the reporter module.The developed biosens...A signal-amplified mercury sensing biosensor with desired sensitivity was developed through firstly using the GFP mutant with fluorescence increasing response towards Hg^2+ as the reporter module.The developed biosensor showed response for Hg^2+ in a relatively wide range of 1–10,000 nmol/L,and the detection limit was improved one or two orders of magnitude in comparison with most metal-sensing biosensors in similar constructs.In addition,the biosensor could distinguish Hg^2+ easily from multiple metal ions and displayed strong adaptability to extensive p H conditions (pH 4.0–10.0).More importantly,the developed biosensor was able to provide an initial assessment of Hg^2+ spiked in the environmental water with the recoveries between 85.70%and 112.50%.The signal-amplified strategy performed by the modified reporter module will be widely applicable to many other wholecell biosensors,meeting the practical requirements with sufficient sensing performance.展开更多
A flow injection preconcentration system for the flame atomic absorption spectrometric determination of hexavalent chromium has been developed. The method employs on-line preconcentration of Cr(VI) on a minicolumn pac...A flow injection preconcentration system for the flame atomic absorption spectrometric determination of hexavalent chromium has been developed. The method employs on-line preconcentration of Cr(VI) on a minicolumn packed with Cr(VI)-imprinted poly(4-vinyl pyridineco-2-hydroxyethyl methacrylate) placed into a flow injection system. Hexava-lent chromium was eluted with a small volume of diluted hydrochloric acid into the nebulizer-burner system of a flame atomic absorption spectrometer. An enrichment factor of 550 and a 3σ detection limit of 0.04 μg·L-1 along a sampling frequency of 4 h-1 at a sample flow rate of 3.5 mL·min-1. The relative standard deviation is 2.9% for 1 μg·L-1 Cr(VI) (n = 11). The flow injection system proposed has the advantage of being simpler because the use of expensive and sophisticated instruments is avoided. Ease of use, continuous process and selectivity make this method suitable for Cr(VI) determination in different environmental samples such as sea and river waters, soils and sediments.展开更多
文摘A modified separation method has been developed for determinating polychlorinated biphenyl congeners in environmental samples. Direct treatment of extract with concentrated HzSO4 was employed in the first step for removal of lipids and other interfering substances, then a joint column of alumina-silica gel(Ag+) was applied to separate PCBs fraction from HCH, DDT and its analogs. After this separation, the PCBs fraction was analyzed by capillary gas chromatography with ECD detector and confirmed by GC/MS. The recoveries of individual congeners in Aroclor 1254 through the separation are about 79%-84%. The method is very efficient and useful for determination of trace amount of PCB congeners in environmental samples.
文摘The lactose-fermenter Enterobacteriaceae are the most frequent cause of clinical infection in our country. The objective of this study was to isolate and identify the most common lactose-fermenter Enterobacteriaceae from clinical samples, including urine, blood, wounds, and sputum, obtained from the local hospital and from environmental samples from a chicken farm, agriculture soil, and water from the Tigris River in Baghdad City. The study also aimed at establishing the antibiotic resistance patterns of the isolated bacteria. A total of 155 bacterial isolates were identified from 10 genera according to the Vitek 2 system. The most common bacterial isolates from the clinical and environmental samples were Escherichia coli and Klebsiella pneumoniae, respectively. The antibiotic resistance patterns showed that all clinical and environmental isolates were multidrug resistant to β-lactam (except carbapenems) drug and aminoglycosides and more sensitive to carbapenems.
文摘This work presents a multi-residue analytical method based on solid phase extraction (SPE) followed by high-performance liquid chromatographic (HPLC) with diode array (DAD) detection for the simultaneous determination of a group of pharmaceutical products that include ten antidepressants and three anticanceri- genic in environmental samples (water and soil). Baseline separation of the studied compounds was obtained on an ultrabase C18 (4.6 mm i.d. × 150 mm, 5 μm particle) column using acetonitrile:phosphate buffer pH 2.5 (35:65 v/v) as mobile phase with a flow rate of 1.5 mL/min. Different aspects including linearity, accuracy, precision and detection and quantification limits were examined in order to validate the proposed method. Detection limits between 1 and 50 ng/mL were obtained for all the target compounds. This method was ap- plied to the analysis of environmental samples as waters and soils of different precedence. Prior, the HPLC determination the samples were purified and enriched using SPE or liquid-liquid extraction (LLE) of the tar- get compounds.
文摘A closed-vessel microwave digestion method is described for the rapid dissolution of environmental samples such as foods, soils and sediments. Depending on the sample type, 0.1-0.2 g sample was decomposed with HNO3/H2O2 or HNO3 / H2O2/HF acid mixture in a PTFE digestion vessel by using microwave heating for 2-3 min at 500W of microwave power. The solution, or to which 0.5 g of boric acid was added, was diluted to 25-50 ml and directly determined by sequential ICP-AES. The accuracy of the procedure was validated by the analysis of six standard reference materials for 10 elements. Ail results were in a good agreements with the certified values.
基金financially supported of the National Natural Science Foundation of China(22006121,22076153)Sichuan Science and Technology Program(2020YFH0048)
文摘Surface-enhanced Raman scattering(SERS)has been widely used in various fields due to high sensitivity,but it is hindered by its low repeatability.Here,we explored a highly reproducible free-standing liquid-state platform(FSM)for monitoring norfloxacin(NOR)in the environmental field.After optimizing the types of nanoparticles(NPs)and pH value in NOR solution,the relative standard deviation(RSD)of FSM SERS analysis can be stabilized below 10%(n≥8),and the R^(2)of the calibration curve in the range of 20μg/L-100μg/L was 0.995.In addition,the results showed that the limit of detection(LOD)(3σ/k)of NOR was 1.3μg/L.Moreover,the NOR in the spiked samples was successfully quantified by FSM,and the recovery rate exceeded 95%.The proposed method has the potential for rapid analysis of drug residues in environmental samples.
基金supported by the State Key Laboratory of NBC Protection for Civilian
文摘Organophosphate nerve agents(OPNAs)are lethal chemical weapons(CWs)which hydrolyze rapidly to alkyl phosphate compounds in the environment.The identification of alkyl phosphate compounds in the environment is critical for the verification of OPNAs.Currently,due to the complex sample preparation and long detection process for organophosphate nerve agent hydrolysis products(OPNAHPs),their high-throughput determination is difficult.To simplify sample preparation and detection of OPNAHPs from environmental samples,a quick,easy,cheap,effective,rugged and safe(Qu ECh ERS)method for the simultaneous purification of the five OPNAHPs in environmental samples combined with detection through direct analysis in real-time ionization source coupled with tandem mass spectrometer(DART-MS/MS)was developed.An internal standard method was applied for the quantification.The method exhibited a good linearity(R^(2)>0.991)in the range of 1.00 ng/m L-4.00μg/m L.The limits of detection and limits of quantification were 1.00-7.00 ng/m L and 10.0-20.0 ng/m L,respectively.The recoveries of OPNAHPs in environmental samples were between 65.3%and 107%,with relative standard deviations ranged from 1.6%to 19%.In addition,the sample preparation and analysis process for OPNAHPs could be completed in less than 10 min.The results demonstrated that the modified Qu ECh ERS method could quickly and efficiently remove the matrix of environmental samples.Combined with DART-MS/MS,an accurate and high-throughput detection method of OPNAHPs in environmental samples was provided.The developed method has great potential in the fields of early warning,clinical treatment and environmental decontamination.
基金Supported by the Basic Scientific Fund for National Public Research Institutes of China(Nos.GY02-2011T10,2015P07)the Qingdao Talent Program(No.13-CX-20)+1 种基金the National Natural Science Foundation of China(Nos.31100567,41176061)the National Natural Science Foundation for Creative Groups(No.41521064)
文摘Detection of sulfur-oxidizing bacteria has largely been dependent on targeted gene sequencing technology or traditional cell cultivation, which usually takes from days to months to carry out. This clearly does not meet the requirements of analysis for time-sensitive samples and/or complicated environmental samples. Since energy-dispersive X-ray spectrometry(EDS) can be used to simultaneously detect multiple elements in a sample, including sulfur, with minimal sample treatment, this technology was applied to detect sulfur-oxidizing bacteria using their high sulfur content within the cell. This article describes the application of scanning electron microscopy imaging coupled with EDS mapping for quick detection of sulfur oxidizers in contaminated environmental water samples, with minimal sample handling. Scanning electron microscopy imaging revealed the existence of dense granules within the bacterial cells, while EDS identified large amounts of sulfur within them. EDS mapping localized the sulfur to these granules. Subsequent 16S rRNA gene sequencing showed that the bacteria detected in our samples belonged to the genus Chromatium, which are sulfur oxidizers. Thus, EDS mapping made it possible to identify sulfur oxidizers in environmental samples based on localized sulfur within their cells, within a short time(within 24 h of sampling). This technique has wide ranging applications for detection of sulfur bacteria in environmental water samples.
基金supported by the Science and Technology Department of Henan Province (082300423202 & 102300410194)
文摘Trace of Pb(Ⅱ) has been on-line separated and enriched from environmental samples and wastewater by using the self-made alizarin violet functionalized silica gel micro-column coupling with a sequential injection sampling technology. The determination is based on the color reaction of Pb(Ⅱ) with iodide and crystal violet to form an ionic association complex in the presence of polyvinyl alcohol and hydrochloric acid. The use of the microcolumn can prevent the interference of most familiar metal ions, and therefore improve the selectivity and sensitivity of this analytical technique. The proposed method was used for the determination of Pb(Ⅱ) in environmental samples and wastewater. No statistically significant difference was observed between the results determined by the present method and atomic absorption spectrometry.
基金supported by Cooperative Research Centres Project(CRCP)awarded to Geneworks and La Trobe University.L.T.is supported by an Australian Research Training Program scholarship and the Tim Healy Memorial Scholarship awarded by The Department of Primary Industries South Australia(PIRSA).
文摘Lymnaeid snails are key intermediate hosts for the development and survival of Fasciola spp.,the causative agent of Fascioliasis which are economically important parasites infecting humans and livestock globally.The current control method for treating Fascioliasis is heavily reliant on anthelmintic drugs,particularly Triclabendazole(TCBZ)which has resulted in drug-resistant parasites and poses significant risk as there are no long-term efficacious alternatives available.Sustainable control measures at the farm level could include both parasite and snail control will play an important role in Fasciola spp.control and reduce the reliance on anthelmintic drugs.Implementation of such sustainable control measures requires effective identification of snails on the property however Lymnaeid snails are small and difficult to physically locate.Snail identification using an environmental DNA approach is a recent approach in which physically locating snails are not required.Austropeplea tomentosa,is the primary intermediate snail host for F.hepatica transmission in South-East Australia and we present an in-field loop-mediated isothermal amplification and water filtering method for the detection of A.tomentosa eDNA from water samples to improve current surveillance methods.This methodology is highly sensitive with a detection limit of 5×10^(−6)ng/μL,detected in<20 minutes,with cumulative sample preparation and amplification time under 1 hour.This proposed workflow could assist in monitoring areas to determine the risk of Fascioliasis infection and implement strategies to manage snail populations to ultimately reduce the risk of infection for humans and livestock.
文摘Based on poly(vinyl chloride) membranes, a novel miniaturized screen-printed all-solid-state copper(II)-selective electrode has been developed for applications in environmental monitoring. Performance and applicability of the ion-selective electrode (ISE) have been proved by potentiometric investigations. Conducting polymers were used as intermediate layers and as solid contacts between the ion-selective membrane and the graphite transducer. The ion-complexing reagent 2-mercapto-benzoxazole was incorporated into poly(vinyl chloride) membranes. In the concentration range 10<sup>-6</sup> - 10<sup>-2</sup> mol/L, the ISE exhibited a linear Nernstian potential response to copper(II) with an average slope value of 28 mV/decade. The detection limit was 3 × 10<sup>-7</sup> mol/L. The electrode exhibits a short response time (<10 s) and can be used in the range of pH = 3 - 7. Selectivity coefficents against certain interfering ions are investigated. The life time of the electrode under laboratory conditions was approximately 12-month. The electrode was applied in the investigation of different aqueous environmental samples and the electrode characteristics were described. The copper(II) ASS electrode has also successfully been used in potentiometric, complexometric titrations with ethylenediaminetetraacetic acid.
基金supported by the Technology Agency of the Czech Republic(Grant Nos.SS01010270,SS06010420)by a long-term research development grant RVO(Grant No.67985939)。
文摘Sequencing of environmental samples has great potential for biodiversity research,but its application is limited by the lack of reliable DNA barcode databases for species identifications.Such a database has been created for epiphytic lichens of Europe,allowing us to compare the results of environmental sequencing with standard taxonomic surveys.The species undetected by taxonomic surveys(what we term the ghost component)amount to about half of the species actually present in hectare plots of Central European forests.Some of these,which currently occur only as diaspores or weakly developed thalli,are likely to be favoured in the course of global change.The ghost component usually represents a larger fraction in managed forests than in old-growth unmanaged forests.The total species composition of different plots is much more similar than suggested by taxonomic surveys alone.On a regional scale,this supports the well-known statement that“everything is everywhere,but,the environment selects”.
基金supported by the Special National Project on investigation of basic resources of China(Grant 2019FY101500)the National Natural Science Foundation of China(Grants 81861138003 and 31930001)supported an Australian Research Council(ARC)Australian Laureate Fellowship(FL170100022)。
文摘Healthcare workers(HCWs)are at high risk of occupational exposure to the new pandemic human coronavirus,SARSCoV-2,and are a source of nosocomial transmission in airborne infectious isolation rooms(AIIRs).Here,we performed comprehensive environmental contamination surveillance to evaluate the risk of viral transmission in AIIRs with 115 rooms in three buildings at the Shanghai Public Health Clinical Center,Shanghai,during the treatment of 334 patients infected with SARS-CoV-2.The results showed that the risk of airborne transmission of SARS-CoV-2 in AIIRs was low(1.62%,25/1544)due to the directional airflow and strong environmental hygiene procedures.However,we detected viral RNA on the surface of foot-operated openers and bathroom sinks in AIIRs(viral load:55.00–3154.50 copies/mL).This might be a source of contamination to connecting corridors and object surfaces through the footwear and gloves used by HCWs.The risk of infection was eliminated by the use of disposable footwear covers and the application of more effective environmental and personal hygiene measures.With the help of effective infection control procedures,none of 290 HCWs was infected when working in the AIIRs at this hospital.This study has provided information pertinent for infection control in AIIRs during the treatment of COVID-19 patients.
基金supported by the Ministry of Education and Science of Spain(No.CTM2005-106457-C05-05/TECNO),FPI grant from Ministry of Education and Science of Spain,and FI grant from the Comissionat per a Universitats i Recerca del Departament d'Innovació,Universitats i Empresa de la Generalitat de Catalunya i del Fons Social Europeu
文摘Helicobacter pylori are ubiquitous Gram-negative bacteria with a high estimated level of infection in the world populations, but a majority of the infected persons are asymptomatic. This pathogen has been classified by the World Health Organization as a class I carcinogen and recognized as the causal agent of most peptic ulcers and chronic gastritis that might lead to stomach cancer. Although not all the transmission pathways of these bacteria into humans have been propedy identified, enough data have suggested that the oral-oral or fecal-oral ones are the main infection routes. Helicobacterpylori have been detected in non-treated water and in drinking water, which suggested that water might be an important infection source. As childhood is the critical period of infection, the aim of the present work was to examine the presence of Helicobacterpylori in soil samples from public playing areas of Spanish parks.
基金supported by NIH award U01 EB0220003-01[CED,NJK]the NIH National Center for Advancing Translational Sciences(NCATS)through grant UL1 TR000002[CED,NJK]+1 种基金NIH award 1P30ES023513-01A1[CED,NJK]NIH award UG3-OD023365[CED,NJK]。
文摘Trace analysis of volatile organic compounds(VOCs) during wildfires is imperative for environmental and health risk assessment. The use of gas sampling devices mounted on unmanned aerial vehicles(UAVs) to chemically sample air during wildfires is of great interest because these devices move freely about their environment, allowing for more representative air samples and the ability to sample areas dangerous or unreachable by humans. This work presents chemical data from air samples obtained in Davis, CA during the most destructive wildfire in California's history-the 2018 Camp Fire – as well as the deployment of our sampling device during a controlled experimental fire while fixed to a UAV. The sampling mechanism was an in-house manufactured micro-gas preconcentrator(μPC) embedded onto a compact battery-operated sampler that was returned to the laboratory for chemical analysis. Compounds commonly observed in wildfires were detected during the Camp Fire using gas chromatography mass spectrometry(GC–MS), including BTEX(benzene, toluene, ethylbenzene, m + p-xylene, and o-xylene), benzaldehyde, 1,4-dichlorobenzene, naphthalene, 1,2,3-trimethylbenzene and 1-ethyl-3-methylbenzene. Concentrations of BTEX were calculated and we observed that benzene and toluene were highest with average concentrations of 4.7 and 15.1 μg/m^(3), respectively. Numerous fire-related compounds including BTEX and aldehydes such as octanal and nonanal were detected upon experimental fire ignition, even at a much smaller sampling time compared to samples taken during the Camp Fire. Analysis of the air samples taken both stationary during the Camp Fire and mobile during an experimental fire show the successful operation of our sampler in a fire environment.
基金supported by the National Key R&D Program of China(No.2019YFC1905400)the National Natural Science Foundation of China(No.22006113)。
文摘Antibiotics,as widely used antibacterial drug,exist in various environmental media.Antibiotic residues can affect biological metabolism and lead to bacterial resistance and the formation of antibiotic-resistance genes,posing a threat to human health and ecological safety.Establishing efficient detection methods for antibiotics and antibiotic-resistance genes has great environmental significance.Fluorescence detection methods,due to their fast response,high sensitivity and specificity,and low-cost,are widely used in chemical and biological sensing.This review first summarizes the pre-treatment methods for different types of environmental samples,and then focuses on the recent advances of fluorescence methods for the detection of antibiotics and antibiotic-resistance genes.Finally,main challenges and future research directions of fluorescence methods for antibiotic and antibiotic-resistance genes detection are discussed.This review highlights the promising prospect of fluorescence methods in-situ detection and monitoring of antibiotics and antibiotic-resistance genes,and provides guidance for the construction of overall risk assessment system of environmental media.
基金Supported by the National Key Research and Development Program of China(2022YFC3204703)the China Postdoctoral Innovation Talents Support Program(BX20230400).
文摘Introduction:The establishment of a highthroughput quantification approach for waterborne pathogenic protozoa and helminths is crucial for rapid screening and health risk assessment.Methods:We developed a high-throughput quantitative polymerase chain reaction(HT-qPCR)assay targeting 19 waterborne protozoa and 3 waterborne helminths and validated its sensitivity,specificity,and repeatability.The assay was then applied to test various environmental media samples.Results:The HT-qPCR assay's limit of detection(LOD)was 5×10^(2) copies/μL DNA,and its specificity was confirmed using Giardia and Cryptosporidium standards.Repeatability,assessed through intra-and inter-group experiments,yielded a coefficient of variation(CV)of 1.0%–4.6%and 1.2%–6.4%at concentrations of 1×10^(5) and 1×10^(4) copies/μL,respectively.The R^(2) values of the 22 standard curves ranged from 0.983 to 0.998,with amplification efficiencies between 80%and 107%.In drinking water sources,sludge from municipal wastewater treatment plants(MWTPs),and livestock manure samples,17 of 22 targets were detected,with Acanthamoeba genus(50.0%),Acanthamoeba castellanii(11.8%),and Enterocytozoon bieneusi(11.8%)showing high prevalence.Cryptosporidium spp.,Enterocytozoon bieneusi,and Cyclospora cayetanensis were simultaneously found in all three sample types.Discussion:This study presents a useful tool for the rapid detection of waterborne protozoa and helminths in complex environmental microbiomes,providing scientific data for monitoring cross-media transmission and controlling microbial risk from a One Health perspective.
基金supported by the Research Foundation of Science and Technology Project in Guangdong Province of China (No.2003C20409)Science and Technology Project in General Administration of Quality Supervision,Inspection and Quarantine of China(No. 2004IK062)
文摘Objective To detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy （GFAAS） and inductively coupled plasma atomic emission spectroscopy （ICPAES）. Methods An indirect competitive enzyme-linked immunosorbent assay （IC-ELISA） was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated. Results IC-ELISA showed an IC50 of 45.6 μg/L with a detection limit of 1.95 μg/L for cadmium, and showed a mean recovery ranging 97.67%-107.08%. The coefficient of variations for intra- and interassay was 3.41%-6.61% and 4.70%-9.21%, respectively. The correlation coefficient between IC-ELISA and GFAAS was 0.998. Conclusion IC-ELISA can detect and quantify cadmium residue in environmental or food samples.
基金supported by the National Natural Science Foundation of China (No.31800631)the Natural Science Foundation of Guangxi Province (No.2018JJB120049)+1 种基金the Middleaged and Young Teachers’ Basic Ability Promotion Project of Guangxi (No.2018KY0361)the BAGUI Scholar Program of Guangxi Province of China。
文摘A signal-amplified mercury sensing biosensor with desired sensitivity was developed through firstly using the GFP mutant with fluorescence increasing response towards Hg^2+ as the reporter module.The developed biosensor showed response for Hg^2+ in a relatively wide range of 1–10,000 nmol/L,and the detection limit was improved one or two orders of magnitude in comparison with most metal-sensing biosensors in similar constructs.In addition,the biosensor could distinguish Hg^2+ easily from multiple metal ions and displayed strong adaptability to extensive p H conditions (pH 4.0–10.0).More importantly,the developed biosensor was able to provide an initial assessment of Hg^2+ spiked in the environmental water with the recoveries between 85.70%and 112.50%.The signal-amplified strategy performed by the modified reporter module will be widely applicable to many other wholecell biosensors,meeting the practical requirements with sufficient sensing performance.
基金This work has been funded by the Spain’s Ministry of Science and Innovation,within the framework of Project CTQ2009-12282.
文摘A flow injection preconcentration system for the flame atomic absorption spectrometric determination of hexavalent chromium has been developed. The method employs on-line preconcentration of Cr(VI) on a minicolumn packed with Cr(VI)-imprinted poly(4-vinyl pyridineco-2-hydroxyethyl methacrylate) placed into a flow injection system. Hexava-lent chromium was eluted with a small volume of diluted hydrochloric acid into the nebulizer-burner system of a flame atomic absorption spectrometer. An enrichment factor of 550 and a 3σ detection limit of 0.04 μg·L-1 along a sampling frequency of 4 h-1 at a sample flow rate of 3.5 mL·min-1. The relative standard deviation is 2.9% for 1 μg·L-1 Cr(VI) (n = 11). The flow injection system proposed has the advantage of being simpler because the use of expensive and sophisticated instruments is avoided. Ease of use, continuous process and selectivity make this method suitable for Cr(VI) determination in different environmental samples such as sea and river waters, soils and sediments.
