Collagens are large secreted trimeric proteins making up most of the animal extracellular matrix.Secretion of collagen has been a focus of interest for cell biologists in recent years because collagen trimers are too ...Collagens are large secreted trimeric proteins making up most of the animal extracellular matrix.Secretion of collagen has been a focus of interest for cell biologists in recent years because collagen trimers are too large and rigid to fit into the COPII vesicles mediating transport from the endoplasmic reticulum（ER） to the Golgi. Collagen-specific mechanisms to create enlarged ER-to-Golgi transport carriers have been postulated, including cargo loading by conserved ER exit site（ERES） protein Tango1.Here, we report an RNAi screening for genes involved in collagen secretion in Drosophila. In this screening, we examined distribution of GFP-tagged Collagen IV in live animals and found 88 gene hits for which the knockdown produced intracellular accumulation of Collagen IV in the fat body, the main source of matrix proteins in the larva. Among these hits, only two affected collagen secretion specifically：PH4 a EFB and Plod, encoding enzymes known to mediate posttranslational modification of collagen in the ER. Every other intracellular accumulation hit affected general secretion, consistent with the notion that secretion of collagen does not use a specific mode of vesicular transport, but the general secretory pathway. Included in our hits are many known players in the eukaryotic secretory machinery, like COPII and COPI components, SNAREs and Rab-GTPase regulators. Our further analysis of the involvement of Rab-GTPases in secretion shows that Rab1, Rab2 and Rab X3, are all required at ERES, each of them differentially affecting ERES morphology. Abolishing activity of all three by Rep knockdown, in contrast,led to uncoupling of ERES and Golgi. We additionally present a characterization of a screening hit we named trabuco（tbc）, encoding an ERES-localized TBC domain-containing Rab-GAP. Finally, we discuss the success of our screening in identifying secretory pathway genes in comparison to two previous secretion screenings in Drosophila S2 cells.展开更多
In this work,the deformation of free interface during water entry and exit of a circular cylinder is investigated numerically by using the two-dimensional(2D)immersed boundary-multiphase lattice Boltzmann flux solver(...In this work,the deformation of free interface during water entry and exit of a circular cylinder is investigated numerically by using the two-dimensional(2D)immersed boundary-multiphase lattice Boltzmann flux solver(IB-MLBFS).The fluid domain is discretized by finite volume discretization,and the flux on the grid interface is evaluated by lattice Boltzmann equations.Both the implicit velocity correction and the surface flux correction are implemented by using the immersed boundary-method to consider the fluid-structure interaction and the contact interface between the multiphase fluids and the structure.First,the water entry of a circular cylinder is simulated and the results are compared with the experiment,which considered the length-diameter ratio of the circular cylinder.The reliability of 2D simulation is verified and the deformation of the free interface is well investigated.Afterward,the water exit of a circular cylinder with constant velocity is simulated,which is less researched.In addition,the results show the advantage of present IB-MLBFS to some extent.Finally,the water exit and re-entry of a circular cylinder are presented,and the results present the complex deformation of the free interface and the dynamic response of the moving structure.Based on the numerical results,the free interface of the multiphase fluids is well captured,and the contact interface on the boundary of the moving structure is accurately presented by the IB-MLBFS.展开更多
In this paper, we consider a unidirectional highway road with one entry/exit uniformly distributed on the road interval. One road side unit (RSU) is located on the position of the entry/exit. We build an analytical mo...In this paper, we consider a unidirectional highway road with one entry/exit uniformly distributed on the road interval. One road side unit (RSU) is located on the position of the entry/exit. We build an analytical model to study the network connectivity problem. In building the analytical model, we take into account several parameters, such as vehicle arrival rate, vehicle moving speed, vehicle communication radius, RSU communication radius, highway road length and the probability of vehicles driving through the entry/exit. The analytical model is verified by using simulation tools.展开更多
Based on extensive micro data sets, this paper examines the relationship among large-scale entry and exit, competition and total factor productivity （TFP） growth of China＇s industry during economic transition and m...Based on extensive micro data sets, this paper examines the relationship among large-scale entry and exit, competition and total factor productivity （TFP） growth of China＇s industry during economic transition and market opening. We have arrived at these findings： market opening spurs entry and exit of large numbers of non-state-owned enterprises （non-SOEs） and facilitates dynamic competition; through the stimulation effect of competition, entry and exit push TFP growth on the part of enterprises; through the resource reallocation effect, entry and exit promote growth of the aggregate TFP. However, results indicate that the resource allocation efficiency remains very low in capital-intensive industries where the government intervenes a lot. To conclude, promoting open competition is significant to the growth of industrial sectors ＇productivity.展开更多
Road traffic flow forecasting provides critical information for the operational management of road mobility challenges, and models are used to generate the forecast. This paper uses a random process to present a novel...Road traffic flow forecasting provides critical information for the operational management of road mobility challenges, and models are used to generate the forecast. This paper uses a random process to present a novel traffic modelling framework for aggregate traffic on urban roads. The main idea is that road traffic flow is random, even for the recurrent flow, such as rush hour traffic, which is predisposed to congestion. Therefore, the structure of the aggregate traffic flow model for urban roads should correlate well with the essential variables of the observed random dynamics of the traffic flow phenomena. The novelty of this paper is the developed framework, based on the Poisson process, the kinematics of urban road traffic flow, and the intermediate modelling approach, which were combined to formulate the model. Empirical data from an urban road in Ghana was used to explore the model’s fidelity. The results show that the distribution from the model correlates well with that of the empirical traffic, providing a strong validation of the new framework and instilling confidence in its potential for significantly improved forecasts and, hence, a more hopeful outlook for real-world traffic management.展开更多
基金funded by grants from the Natural Science Foundation of China (Nos.31771600 and 31750410689)Tsinghua Initiative Program (No.20131089281)a 1000 Talents award,all to J.C.P.-P
文摘Collagens are large secreted trimeric proteins making up most of the animal extracellular matrix.Secretion of collagen has been a focus of interest for cell biologists in recent years because collagen trimers are too large and rigid to fit into the COPII vesicles mediating transport from the endoplasmic reticulum（ER） to the Golgi. Collagen-specific mechanisms to create enlarged ER-to-Golgi transport carriers have been postulated, including cargo loading by conserved ER exit site（ERES） protein Tango1.Here, we report an RNAi screening for genes involved in collagen secretion in Drosophila. In this screening, we examined distribution of GFP-tagged Collagen IV in live animals and found 88 gene hits for which the knockdown produced intracellular accumulation of Collagen IV in the fat body, the main source of matrix proteins in the larva. Among these hits, only two affected collagen secretion specifically：PH4 a EFB and Plod, encoding enzymes known to mediate posttranslational modification of collagen in the ER. Every other intracellular accumulation hit affected general secretion, consistent with the notion that secretion of collagen does not use a specific mode of vesicular transport, but the general secretory pathway. Included in our hits are many known players in the eukaryotic secretory machinery, like COPII and COPI components, SNAREs and Rab-GTPase regulators. Our further analysis of the involvement of Rab-GTPases in secretion shows that Rab1, Rab2 and Rab X3, are all required at ERES, each of them differentially affecting ERES morphology. Abolishing activity of all three by Rep knockdown, in contrast,led to uncoupling of ERES and Golgi. We additionally present a characterization of a screening hit we named trabuco（tbc）, encoding an ERES-localized TBC domain-containing Rab-GAP. Finally, we discuss the success of our screening in identifying secretory pathway genes in comparison to two previous secretion screenings in Drosophila S2 cells.
基金Supported by the National Natural Science Foundation of China(52061135107)the Fundamental Research Fund for the Central Universities(DUT20TD108,DUT20LAB308)the Liao Ning Revitalization Talents Program(XLYC1908027),and Dalian Innovation Research Team in Key Areas(2020RT03).
文摘In this work,the deformation of free interface during water entry and exit of a circular cylinder is investigated numerically by using the two-dimensional(2D)immersed boundary-multiphase lattice Boltzmann flux solver(IB-MLBFS).The fluid domain is discretized by finite volume discretization,and the flux on the grid interface is evaluated by lattice Boltzmann equations.Both the implicit velocity correction and the surface flux correction are implemented by using the immersed boundary-method to consider the fluid-structure interaction and the contact interface between the multiphase fluids and the structure.First,the water entry of a circular cylinder is simulated and the results are compared with the experiment,which considered the length-diameter ratio of the circular cylinder.The reliability of 2D simulation is verified and the deformation of the free interface is well investigated.Afterward,the water exit of a circular cylinder with constant velocity is simulated,which is less researched.In addition,the results show the advantage of present IB-MLBFS to some extent.Finally,the water exit and re-entry of a circular cylinder are presented,and the results present the complex deformation of the free interface and the dynamic response of the moving structure.Based on the numerical results,the free interface of the multiphase fluids is well captured,and the contact interface on the boundary of the moving structure is accurately presented by the IB-MLBFS.
文摘In this paper, we consider a unidirectional highway road with one entry/exit uniformly distributed on the road interval. One road side unit (RSU) is located on the position of the entry/exit. We build an analytical model to study the network connectivity problem. In building the analytical model, we take into account several parameters, such as vehicle arrival rate, vehicle moving speed, vehicle communication radius, RSU communication radius, highway road length and the probability of vehicles driving through the entry/exit. The analytical model is verified by using simulation tools.
文摘Based on extensive micro data sets, this paper examines the relationship among large-scale entry and exit, competition and total factor productivity （TFP） growth of China＇s industry during economic transition and market opening. We have arrived at these findings： market opening spurs entry and exit of large numbers of non-state-owned enterprises （non-SOEs） and facilitates dynamic competition; through the stimulation effect of competition, entry and exit push TFP growth on the part of enterprises; through the resource reallocation effect, entry and exit promote growth of the aggregate TFP. However, results indicate that the resource allocation efficiency remains very low in capital-intensive industries where the government intervenes a lot. To conclude, promoting open competition is significant to the growth of industrial sectors ＇productivity.
文摘[研究目的]解决多源数据融合过程中参与者贡献与收益的匹配问题、参与者选择缺乏灵活性以及联邦系统的动态适应性不足问题,提升多源数据融合的公平性和合理性。[研究方法]提出一种基于动态自适应联邦学习的多源数据融合框架(Federated Learning and Dynamic Improvement,FLDI),并设计预算分配机制、参与者选择机制以及参与者动态进出机制确保多源数据融合过程的安全、公平和可持续。分别在分类任务的专利、论文以及媒体数据集和预测任务的MNIST、FMNIST和CIFAR-10数据集上展开性能测试,并在不同场景中评估框架性能。[研究结果/结论]FLDI在面对复杂场景时,其准确率相较于FedAvg和FedProx提升了3%~4%;在干净数据集场景下,FLDI在分类任务的平均准确率达到67.01%,在预测任务的平均准确率达到81.56%;进行增强实验后,FLDI在分类任务的平均准确率上升了4.54%,在预测任务的平均准确率上升了3.31%;FLDI框架在分类任务和预测任务中较之FedAvg和FedProx更具性能优势。
文摘Road traffic flow forecasting provides critical information for the operational management of road mobility challenges, and models are used to generate the forecast. This paper uses a random process to present a novel traffic modelling framework for aggregate traffic on urban roads. The main idea is that road traffic flow is random, even for the recurrent flow, such as rush hour traffic, which is predisposed to congestion. Therefore, the structure of the aggregate traffic flow model for urban roads should correlate well with the essential variables of the observed random dynamics of the traffic flow phenomena. The novelty of this paper is the developed framework, based on the Poisson process, the kinematics of urban road traffic flow, and the intermediate modelling approach, which were combined to formulate the model. Empirical data from an urban road in Ghana was used to explore the model’s fidelity. The results show that the distribution from the model correlates well with that of the empirical traffic, providing a strong validation of the new framework and instilling confidence in its potential for significantly improved forecasts and, hence, a more hopeful outlook for real-world traffic management.
