Residual antibiotics in chicken manure may interfere with the stability of anaerobic digestion(AD)and inhibit resource utilization efficiency.In this study,we aimed to enhance chicken manure bio-methanation in AD with...Residual antibiotics in chicken manure may interfere with the stability of anaerobic digestion(AD)and inhibit resource utilization efficiency.In this study,we aimed to enhance chicken manure bio-methanation in AD with highconcentration of sulfamethazine(SMZ)by adding metal(Fe,Co)-modified Enteromorpha-based hydrochar(Co-HC,Fe-HC).The results showed that Fe-HC and Co-HC increased the degree of acidogenesis by 1.25 times and 1.58 times,respectively.The maximum protein concentration in EPS was increased by 47.64%and 72.5%after adding Fe-HC and Co-HC.However,only Co-HC demonstrated notable improvements in both methane production and SMZ removal efficiency.Electrochemical analysis showed that Co-HC possessed a richer variety of oxygen and nitrogen functional groups,along with superior electron exchange capabilities compared to Fe-HC.Furthermore,microbiological assessments revealed that Co-HC enriched syntrophic bacteria(such as Syntrophomonas and Mesotoga),facilitating direct interspecies electron transfer(DIET)and subsequently enhancing biomethane production.The abundance of genes involved in electron transfer increased significantly with Co-HC,with a maximum increase of 75.86%in Co1.5-HC treatment.Additionally,the elimination of antibiotic resistance genes(sul1,sul2)increased by 65.66%in the Co1.5-HC treatment.This study offers a theoretical foundation and empirical support for the synergistic improvement of livestock and poultry manure containing high antibiotic concentrations,thereby helping to overcome challenges posed by recalcitrant substances.展开更多
基金supported by the National High Technology Research and Development Program of China(2022YFC3700187)National Natural Science Foundation of China(U1806216 and 51608304)Young Scholars Program of Shandong University(2018WLJH53).
文摘Residual antibiotics in chicken manure may interfere with the stability of anaerobic digestion(AD)and inhibit resource utilization efficiency.In this study,we aimed to enhance chicken manure bio-methanation in AD with highconcentration of sulfamethazine(SMZ)by adding metal(Fe,Co)-modified Enteromorpha-based hydrochar(Co-HC,Fe-HC).The results showed that Fe-HC and Co-HC increased the degree of acidogenesis by 1.25 times and 1.58 times,respectively.The maximum protein concentration in EPS was increased by 47.64%and 72.5%after adding Fe-HC and Co-HC.However,only Co-HC demonstrated notable improvements in both methane production and SMZ removal efficiency.Electrochemical analysis showed that Co-HC possessed a richer variety of oxygen and nitrogen functional groups,along with superior electron exchange capabilities compared to Fe-HC.Furthermore,microbiological assessments revealed that Co-HC enriched syntrophic bacteria(such as Syntrophomonas and Mesotoga),facilitating direct interspecies electron transfer(DIET)and subsequently enhancing biomethane production.The abundance of genes involved in electron transfer increased significantly with Co-HC,with a maximum increase of 75.86%in Co1.5-HC treatment.Additionally,the elimination of antibiotic resistance genes(sul1,sul2)increased by 65.66%in the Co1.5-HC treatment.This study offers a theoretical foundation and empirical support for the synergistic improvement of livestock and poultry manure containing high antibiotic concentrations,thereby helping to overcome challenges posed by recalcitrant substances.
