Micro/nanorobots(MNRs)are envisioned to provide revolutionary changes to therapies for infectious diseases as they can deliver various antibacterial agents or energies to many hard-to-reach infection sites.However,exi...Micro/nanorobots(MNRs)are envisioned to provide revolutionary changes to therapies for infectious diseases as they can deliver various antibacterial agents or energies to many hard-to-reach infection sites.However,existing MNRs face substantial challenges in addressing complex infections that progress from superficial to deep tissues.Here,we develop swarming magnetic Fe3O4@polydopamine-tannic acid nanorobots(Fe3O4@PDA-TA NRs)capable of performing targeted bacteria elimination in complicated bacterial infections by integrating superficial photothermal and deep chemical strategies.The Fe3O4@PDA-TA nanoparticles(NPs),serving as building blocks of the nanorobots,are fabricated by in situ polymerization of dopamine followed by TA adhesion.When driven by alternating magnetic fields,Fe3O4@PDA-TA NPs can assemble into large energetic microswarms continuously flowing forward with tunable velocity.Thus,the swarming Fe3O4@PDA-TA NRs can be navigated to achieve rapid broad coverage of a targeted superficial area from a distance and rapidly eradicate bacteria residing there upon exposure to near-infrared(NIR)light due to their efficient photothermal conversion.Additionally,they can concentrate at deep infection sites by traversing through confined,narrow,and tortuous passages,exerting sustained antibacterial action through their surface TA-induced easy cell adhesion and subsequent membrane destruction.Therefore,the swarming Fe3O4@PDA-TA NRs show great potential for addressing complex superficial-to-deep infections.This study may inspire the development of future therapeutic microsystems for various diseases with multifunction synergies,task flexibility,and high efficiency.展开更多
This study developed a method for sensitive detection and effective elimination of Listeria monocytogenes(L.monocytogenes)in food products.L.monocytogenes is one of the most threatening foodborne pathogens around the ...This study developed a method for sensitive detection and effective elimination of Listeria monocytogenes(L.monocytogenes)in food products.L.monocytogenes is one of the most threatening foodborne pathogens around the world,posing a serious threat to food safety and public health.Therefore,the detection and effective elimination of L.monocytogenes is significant for ensuring food safety and preventing secondary contamination.Herein,a bifunctional gold carbon dots-silver nanoclusters(GCDs-AgNCs)nanocomposite with excellent fluorescence properties and antimicrobial activity was synthesized in this study.On the one hand,a fluorescence biosensor for the detection of L.monocytogenes by sandwich method was constructed with magnetic separation technique and aptamer-modified GCDs-AgNCs.The biosensor exhibited great detection performance for L.monocytogenes in spiked samples with a limit of detection of 160 CFU/mL.On the other hand,GCDs-AgNCs with antimicrobial activity can eliminate L.monocytogenes by destroying the bacterial cell structure and affecting bacterial metabolism,which effectively avoids secondary contamination.Therefore,this bifunctional nanomaterial can provide a referable new strategy for the rapid analysis and effective elimination of foodborne pathogens.展开更多
基金supported by the National Key Research and Development Project(no.2021YFA1201400)the National Natural Science Foundation of China(no.52073222)the Innovation Team in Key Areas of the Innovation Talent Promotion Plan(2021)of MOST of China.
文摘Micro/nanorobots(MNRs)are envisioned to provide revolutionary changes to therapies for infectious diseases as they can deliver various antibacterial agents or energies to many hard-to-reach infection sites.However,existing MNRs face substantial challenges in addressing complex infections that progress from superficial to deep tissues.Here,we develop swarming magnetic Fe3O4@polydopamine-tannic acid nanorobots(Fe3O4@PDA-TA NRs)capable of performing targeted bacteria elimination in complicated bacterial infections by integrating superficial photothermal and deep chemical strategies.The Fe3O4@PDA-TA nanoparticles(NPs),serving as building blocks of the nanorobots,are fabricated by in situ polymerization of dopamine followed by TA adhesion.When driven by alternating magnetic fields,Fe3O4@PDA-TA NPs can assemble into large energetic microswarms continuously flowing forward with tunable velocity.Thus,the swarming Fe3O4@PDA-TA NRs can be navigated to achieve rapid broad coverage of a targeted superficial area from a distance and rapidly eradicate bacteria residing there upon exposure to near-infrared(NIR)light due to their efficient photothermal conversion.Additionally,they can concentrate at deep infection sites by traversing through confined,narrow,and tortuous passages,exerting sustained antibacterial action through their surface TA-induced easy cell adhesion and subsequent membrane destruction.Therefore,the swarming Fe3O4@PDA-TA NRs show great potential for addressing complex superficial-to-deep infections.This study may inspire the development of future therapeutic microsystems for various diseases with multifunction synergies,task flexibility,and high efficiency.
基金Research Project of State Key Laboratory of Food Science and Resources,Nanchang University(Project No.SKLF-ZZB-202328)for financial support.
文摘This study developed a method for sensitive detection and effective elimination of Listeria monocytogenes(L.monocytogenes)in food products.L.monocytogenes is one of the most threatening foodborne pathogens around the world,posing a serious threat to food safety and public health.Therefore,the detection and effective elimination of L.monocytogenes is significant for ensuring food safety and preventing secondary contamination.Herein,a bifunctional gold carbon dots-silver nanoclusters(GCDs-AgNCs)nanocomposite with excellent fluorescence properties and antimicrobial activity was synthesized in this study.On the one hand,a fluorescence biosensor for the detection of L.monocytogenes by sandwich method was constructed with magnetic separation technique and aptamer-modified GCDs-AgNCs.The biosensor exhibited great detection performance for L.monocytogenes in spiked samples with a limit of detection of 160 CFU/mL.On the other hand,GCDs-AgNCs with antimicrobial activity can eliminate L.monocytogenes by destroying the bacterial cell structure and affecting bacterial metabolism,which effectively avoids secondary contamination.Therefore,this bifunctional nanomaterial can provide a referable new strategy for the rapid analysis and effective elimination of foodborne pathogens.
