Objective:To predict the nephrotoxicity mechanism of Lianqiao-4 through network pharmacology and molecular docking methods.Methods:The main chemical components of Lianqiao(Forsythia suspensa),Bistortae rhizoma,Ophiopo...Objective:To predict the nephrotoxicity mechanism of Lianqiao-4 through network pharmacology and molecular docking methods.Methods:The main chemical components of Lianqiao(Forsythia suspensa),Bistortae rhizoma,Ophiopogonis radix,and Clematidis radix et rhizoma,as well as nephrotoxicity-related targets,were screened through databases such as TCMSP,Swiss Target Prediction,GeneCards,and ETCM.Venny 2.1.0 was used to identify the main components of Lianqiao-4 and nephrotoxicity targets.The STRING platform and David database were utilized to construct a protein-protein interaction(PPI)network diagram,while gene function(GO)enrichment analysis and KEGG pathway analysis were conducted.The“Lianqiao-4 active ingredients-nephrotoxicity targets-signaling pathways”network model was constructed using Cytoscape 3.9.1 software.Results:Network pharmacology and molecular docking analysis revealed that the core active ingredients responsible for the nephrotoxicity mechanism of Mongolian medicine Lianqiao-4 include steroidal saponins such as ophiopogonin A,flavonoids like kaempferol and quercetin,steroidal compounds such asβ-sitosterol and sitosterol,and other key regulatory targets including STAT3,ABCG2,HSP90AA1,MMP9,PTGS2,and EGFR.Major pathways involved include lipid and atherosclerosis,chemical carcinogenesis-DNA adducts,and arachidonic acid metabolism.Conclusion:Mongolian medicine Lianqiao-4 exerts its therapeutic effect on nephrotoxicity through multiple components,targets,and pathways,pending experimental verification.展开更多
(±)-Penicithrones A–D(1a/1b–4a/4b),four novel pairs of anthrone–cyclopentenone heterodimers characterized by a distinctive bridged 6/6/6−5 tetracyclic core skeleton,together with three previously identified co...(±)-Penicithrones A–D(1a/1b–4a/4b),four novel pairs of anthrone–cyclopentenone heterodimers characterized by a distinctive bridged 6/6/6−5 tetracyclic core skeleton,together with three previously identified compounds(5–7),were isolated from the crude extract of the mangrove-derived fungus Penicillium sp.,guided by heteronuclear single quantum correlation(HSQC)-based small molecule accurate recognition technology(SMART 2.0)and liquid chromatography-tandem mass spectrometry(LC-MS/MS)-based molecular networking.The structural elucidation of new compounds was accomplished through comprehensive spectroscopic analysis,and their absolute configurations were determined using DP4+^(13)C nuclear magnetic resonance(NMR)calculations and electronic circular dichroism(ECD)calculations.Compounds 1a/1b–4a/4b demonstrated moderate cytotoxicity against three human cancer cell lines HeLa,HCT116 and MCF-7 with half maximal inhibitory concentration(IC50)values ranging from 15.95±1.64 to 28.56±2.59μmol·L–1.展开更多
With the arrival of the 4G and 5G,the telecommunications networks have experienced a large expansion of these networks.That enabled the integration of many services and adequate flow,thus enabling the operators to res...With the arrival of the 4G and 5G,the telecommunications networks have experienced a large expansion of these networks.That enabled the integration of many services and adequate flow,thus enabling the operators to respond to the growing demand of users.This rapid evolution has given the operators to adapt,their methods to the new technologies that increase.This complexity becomes more important,when these networks include several technologies to access different from the heterogeneous network like in the 4G network.The dimensional new challenges tell the application and the considerable increase in demand for services and the compatibility with existing networks,the management of mobility intercellular of users and it offers a better quality of services.Thus,the proposed solution to meet these new requirements is the sizing of the EPC(Evolved Packet Core)core network to support the 5G access network.For the case of Orange Guinea,this involves setting up an architecture for interconnecting the core networks of Sonfonia and Camayenne.The objectives of our work are of two orders:(1)to propose these solutions and recommendations for the heart network EPC sizing and the deployment to be adopted;(2)supply and architectural interconnection in the heart network EPC and an existing heart network.In our work,the model of traffic in communication that we use to calculate the traffic generated with each technology has link in the network of the heart.展开更多
KTi_(2)(PO_(4))_(3)is a promising anode material for potassium storage,but suffers from low conductivity and difficult balance between high capacity and good structural stability.Herein,the Ti_(3)C_(2)T_(x)MXene is us...KTi_(2)(PO_(4))_(3)is a promising anode material for potassium storage,but suffers from low conductivity and difficult balance between high capacity and good structural stability.Herein,the Ti_(3)C_(2)T_(x)MXene is used as a multifunctional binder to fabricate the KTi_(2)(PO_(4))_(3)electrode by the traditional homogenizing-coating method.The MXene nanosheets,together with the conductive agent super P nanoparticles,construct a multiple conductive network for fast electron/ion transfer and high electrochemical kinet-ics.Moreover,the network ensures the structural stability of the KTi_(2)(PO_(4))_(3)electrode during the de-intercalation/intercalation of 4 K^(+)ions,which is beneficial for simultaneously achieving high capacity and good cycle performance.Therefore,the MXene-bonded KTi_(2)(PO_(4))_(3)electrode delivers a reversible capacity of 255.2 mAh/g at 50 mA/g,outstanding rate capability with 132.3 mAh/g at 2 A/g,and ex-cellent cycle performance with 151.6 mAh/g at 1 A/g after 2000 cycles.This work not only suggests a high-performance anode material for potassium-ion batteries,but also demonstrates that the MXene is a promising binder material for constructing conductive electrodes in rechargeable batteries.展开更多
Salt stress is a global constraint on agricultural production.Therefore,the development of salt tolerant plants has become a current research hotspot.While salt tolerance has evolved more frequently in C_(4) grass lin...Salt stress is a global constraint on agricultural production.Therefore,the development of salt tolerant plants has become a current research hotspot.While salt tolerance has evolved more frequently in C_(4) grass lineages,few studies have explored the molecular bases underlying salt stress tolerance in the C_(4) crop foxtail millet.In this study,we used a multi-pronged approach spanning the omics analyses of transcriptomes and physiological analysis of the C_(3) crop rice and the C_(4) model crop foxtail millet to investigate their responses to salt stress.The results revealed that compared to C_(3) rice,C_(4) foxtail millet has upregulated abscisic acid(ABA)and notably reduced CK biosynthesis and signaling transduction under salt stress.Salt stress in C_(3) rice plants triggered rapid downregulation of photosynthesis related genes,which was coupled with severely reduced net photosynthetic rates.In the salt-treated C_(3) rice and C_(4) foxtail millet,some stress responsive transcription factors(TFs),such as AP2/ERF,WRKY and MYB,underwent strong and distinct transcriptional changes.Based on a weighted gene co-expression network analysis(WGCNA),the AP2/ERF transcription factor Rice Starch Regulator1 SiRSR1(Seita.3G044600)was identified as a key regulator of the salt stress response.To confirm its function,we generated OsRSR1-knockout lines using CRISPR/Cas9 genome editing in rice and its upstream repressor SimiR172a-overexpressing(172a-OE)transgenic plants in foxtail millet,which both showed increased salt tolerance.Overall,this study not only provides new insights into the convergent regulation of the salt stress responses of foxtail millet and rice,but it also sheds light on the divergent signaling networks between them in response to salt stress.展开更多
This article introduces a cable-free real-time telemetry seismic acquisition system(hereinafter referred to as the cable-free real-time telemetry system)that utilizes 4G/5G technology.This system facilitates the real-...This article introduces a cable-free real-time telemetry seismic acquisition system(hereinafter referred to as the cable-free real-time telemetry system)that utilizes 4G/5G technology.This system facilitates the real-time acquisition and quality control of seismic data,the real-time monitoring of equipment location and health status,the synchronous transmission of collected data between the cloud and client,and the real-time issuance of operational instructions.It addresses the critical limitation of existing seismic node equipment,which is often restricted to mining and blind storage due to the absence of a wired or wireless communication link between the acquisition node device and the central control unit.This limitation necessitates local data storage and rendering real-time quality control unfeasible.Typically,quality control is conducted post-task completion,requiring the overall retrieval and downloading of data.If data issues are identifi ed,it becomes necessary to eliminate faulty tracks and determine the need for supplementary acquisition,which can lead to delays in the acquisition process.The implementation of real-time monitoring and early warning systems for equipment health status aims to mitigate the risk of poor data quality resulting from equipment anomalies.Furthermore,the real-time synchronous transmission between the cloud and server addresses the bottleneck of slow download speeds associated with the centralized retrieval of data from multiple node devices during blind acquisition and storage.A real-time microseismic data acquisition test and verifi cation were conducted at a fracturing site in an eastern oil and gas fi eld.Analysis of the test data indicates that the overall performance indicators of the system are comparable to those of existing mainstream system equipment,demonstrating stability and reliability.The performance parameters fully satisfy the technical requirements for oilfield fracturing monitoring scenarios,suggesting promising prospects for further promotion and application.展开更多
This paper presents an investigation of the tribological performance of AA2024–B_(4)C composites,with a specific focus on the influence of reinforcement and processing parameters.In this study three input parameters ...This paper presents an investigation of the tribological performance of AA2024–B_(4)C composites,with a specific focus on the influence of reinforcement and processing parameters.In this study three input parameters were varied:B_(4)C weight percentage,milling time,and normal load,to evaluate their effects on two output parameters:wear loss and the coefficient of friction.AA2024 alloy was used as the matrix alloy,while B_(4)C particles were used as reinforcement.Due to the high hardness and wear resistance of B_(4)C,the optimized composite shows strong potential for use in aerospace structural elements and automotive brake components.The optimisation of tribological behaviour was conducted using a Taguchi-Grey Relational Analysis(Taguchi-GRA)and the Technique for Order of Preference by Similarity to Ideal Solution(TOPSIS).A total of 27 combinations of input parameters were analysed,varying the B_(4)C content(0,10,and 15 wt.%),milling time(0,15,and 25 h),and normal load(1,5,and 10 N).Wear loss and the coefficient of friction were numerically evaluated and selected as criteria for optimisation.Artificial Neural Networks(ANNs)were also applied for two outputs simultaneously.TOPSIS identified Alternative 1 as the optimal solution,confirming the results obtained using the Taguchi Grey method.The optimal condition obtained(10 wt.%B_(4)C,25 h milling time,10 N load)resulted in a minimum wear loss of 1.7 mg and a coefficient of friction of 0.176,confirming significant enhancement in tribological behaviour.Based on the results,both the B_(4)C content and the applied processing conditions have a significant impact on wear loss and frictional properties.This approach demonstrates high reliability and confidence,enabling the design of future composite materials with optimal properties for specific applications.展开更多
LiMnxFe1-xPO_(4) is a promising cathode candidate due to its high security and the availability of a high 4.1 V operating voltage and high energy density.However,the poor electrochemical kinetics and structural instab...LiMnxFe1-xPO_(4) is a promising cathode candidate due to its high security and the availability of a high 4.1 V operating voltage and high energy density.However,the poor electrochemical kinetics and structural instability currently hinder its broader application.Herein,inspired by the hydrogen-bonded cross-linking and steric hindrance effect between short-chain polymer molecules(polyethylene glycol-400,PEG-400),the pomegranate-type LiMn_(0.5)Fe_(0.5)PO_(4)-0.5@C(P-LMFP@C)cathode materials with 3D ion/electron dual-conductive network structure were constructed through ball mill-assisted spray-drying method.The intermolecular effects of PEG-400 promote the spheroidization and uniform PEG coating of LMFP precursor,which prevents agglomeration during sintering.The 3D ion/electron dual-conductive network structure in P-LMFP@C accelerates the Li^(+)transport kinetics,improving the rate performance and cycling stability.As a result,the designed P-LMFP@C has remarkable electrochemical behavior,boasting excellent capacity retention(98%after 100 cycles at the 1C rate)and rate capability(91 mAh·g^(-1)at 20C).Such strategy introduces a novel window for designing high-performance olivine cathodes and offers compatibility with a range of energy storage materials for diverse applications.展开更多
文摘Objective:To predict the nephrotoxicity mechanism of Lianqiao-4 through network pharmacology and molecular docking methods.Methods:The main chemical components of Lianqiao(Forsythia suspensa),Bistortae rhizoma,Ophiopogonis radix,and Clematidis radix et rhizoma,as well as nephrotoxicity-related targets,were screened through databases such as TCMSP,Swiss Target Prediction,GeneCards,and ETCM.Venny 2.1.0 was used to identify the main components of Lianqiao-4 and nephrotoxicity targets.The STRING platform and David database were utilized to construct a protein-protein interaction(PPI)network diagram,while gene function(GO)enrichment analysis and KEGG pathway analysis were conducted.The“Lianqiao-4 active ingredients-nephrotoxicity targets-signaling pathways”network model was constructed using Cytoscape 3.9.1 software.Results:Network pharmacology and molecular docking analysis revealed that the core active ingredients responsible for the nephrotoxicity mechanism of Mongolian medicine Lianqiao-4 include steroidal saponins such as ophiopogonin A,flavonoids like kaempferol and quercetin,steroidal compounds such asβ-sitosterol and sitosterol,and other key regulatory targets including STAT3,ABCG2,HSP90AA1,MMP9,PTGS2,and EGFR.Major pathways involved include lipid and atherosclerosis,chemical carcinogenesis-DNA adducts,and arachidonic acid metabolism.Conclusion:Mongolian medicine Lianqiao-4 exerts its therapeutic effect on nephrotoxicity through multiple components,targets,and pathways,pending experimental verification.
基金supported by the National Key Research and Development Program of China(No.2022YFC2303100)the National Natural Science Foundation of China(Nos.32022002 and 21977113).
文摘(±)-Penicithrones A–D(1a/1b–4a/4b),four novel pairs of anthrone–cyclopentenone heterodimers characterized by a distinctive bridged 6/6/6−5 tetracyclic core skeleton,together with three previously identified compounds(5–7),were isolated from the crude extract of the mangrove-derived fungus Penicillium sp.,guided by heteronuclear single quantum correlation(HSQC)-based small molecule accurate recognition technology(SMART 2.0)and liquid chromatography-tandem mass spectrometry(LC-MS/MS)-based molecular networking.The structural elucidation of new compounds was accomplished through comprehensive spectroscopic analysis,and their absolute configurations were determined using DP4+^(13)C nuclear magnetic resonance(NMR)calculations and electronic circular dichroism(ECD)calculations.Compounds 1a/1b–4a/4b demonstrated moderate cytotoxicity against three human cancer cell lines HeLa,HCT116 and MCF-7 with half maximal inhibitory concentration(IC50)values ranging from 15.95±1.64 to 28.56±2.59μmol·L–1.
文摘With the arrival of the 4G and 5G,the telecommunications networks have experienced a large expansion of these networks.That enabled the integration of many services and adequate flow,thus enabling the operators to respond to the growing demand of users.This rapid evolution has given the operators to adapt,their methods to the new technologies that increase.This complexity becomes more important,when these networks include several technologies to access different from the heterogeneous network like in the 4G network.The dimensional new challenges tell the application and the considerable increase in demand for services and the compatibility with existing networks,the management of mobility intercellular of users and it offers a better quality of services.Thus,the proposed solution to meet these new requirements is the sizing of the EPC(Evolved Packet Core)core network to support the 5G access network.For the case of Orange Guinea,this involves setting up an architecture for interconnecting the core networks of Sonfonia and Camayenne.The objectives of our work are of two orders:(1)to propose these solutions and recommendations for the heart network EPC sizing and the deployment to be adopted;(2)supply and architectural interconnection in the heart network EPC and an existing heart network.In our work,the model of traffic in communication that we use to calculate the traffic generated with each technology has link in the network of the heart.
基金support by National Natural Science Foundation of China(No.U2004212).
文摘KTi_(2)(PO_(4))_(3)is a promising anode material for potassium storage,but suffers from low conductivity and difficult balance between high capacity and good structural stability.Herein,the Ti_(3)C_(2)T_(x)MXene is used as a multifunctional binder to fabricate the KTi_(2)(PO_(4))_(3)electrode by the traditional homogenizing-coating method.The MXene nanosheets,together with the conductive agent super P nanoparticles,construct a multiple conductive network for fast electron/ion transfer and high electrochemical kinet-ics.Moreover,the network ensures the structural stability of the KTi_(2)(PO_(4))_(3)electrode during the de-intercalation/intercalation of 4 K^(+)ions,which is beneficial for simultaneously achieving high capacity and good cycle performance.Therefore,the MXene-bonded KTi_(2)(PO_(4))_(3)electrode delivers a reversible capacity of 255.2 mAh/g at 50 mA/g,outstanding rate capability with 132.3 mAh/g at 2 A/g,and ex-cellent cycle performance with 151.6 mAh/g at 1 A/g after 2000 cycles.This work not only suggests a high-performance anode material for potassium-ion batteries,but also demonstrates that the MXene is a promising binder material for constructing conductive electrodes in rechargeable batteries.
基金supported by the National Natural Science Foundation of China(32241042)the National Key R&D Program of China(2019YFD1000700 and 2019YFD1000703)the Biological Breeding-National Science and Technology Major Project,China(2022ZD04017).
文摘Salt stress is a global constraint on agricultural production.Therefore,the development of salt tolerant plants has become a current research hotspot.While salt tolerance has evolved more frequently in C_(4) grass lineages,few studies have explored the molecular bases underlying salt stress tolerance in the C_(4) crop foxtail millet.In this study,we used a multi-pronged approach spanning the omics analyses of transcriptomes and physiological analysis of the C_(3) crop rice and the C_(4) model crop foxtail millet to investigate their responses to salt stress.The results revealed that compared to C_(3) rice,C_(4) foxtail millet has upregulated abscisic acid(ABA)and notably reduced CK biosynthesis and signaling transduction under salt stress.Salt stress in C_(3) rice plants triggered rapid downregulation of photosynthesis related genes,which was coupled with severely reduced net photosynthetic rates.In the salt-treated C_(3) rice and C_(4) foxtail millet,some stress responsive transcription factors(TFs),such as AP2/ERF,WRKY and MYB,underwent strong and distinct transcriptional changes.Based on a weighted gene co-expression network analysis(WGCNA),the AP2/ERF transcription factor Rice Starch Regulator1 SiRSR1(Seita.3G044600)was identified as a key regulator of the salt stress response.To confirm its function,we generated OsRSR1-knockout lines using CRISPR/Cas9 genome editing in rice and its upstream repressor SimiR172a-overexpressing(172a-OE)transgenic plants in foxtail millet,which both showed increased salt tolerance.Overall,this study not only provides new insights into the convergent regulation of the salt stress responses of foxtail millet and rice,but it also sheds light on the divergent signaling networks between them in response to salt stress.
基金funded by the National Natural Science Foundation of China (42074127)the Key Program of National Natural Science Foundation of China (41930425)Research on Key Technologies for the Production, Exploration, and Development of Continental Shale Oil (2023ZZ15YJ02)。
文摘This article introduces a cable-free real-time telemetry seismic acquisition system(hereinafter referred to as the cable-free real-time telemetry system)that utilizes 4G/5G technology.This system facilitates the real-time acquisition and quality control of seismic data,the real-time monitoring of equipment location and health status,the synchronous transmission of collected data between the cloud and client,and the real-time issuance of operational instructions.It addresses the critical limitation of existing seismic node equipment,which is often restricted to mining and blind storage due to the absence of a wired or wireless communication link between the acquisition node device and the central control unit.This limitation necessitates local data storage and rendering real-time quality control unfeasible.Typically,quality control is conducted post-task completion,requiring the overall retrieval and downloading of data.If data issues are identifi ed,it becomes necessary to eliminate faulty tracks and determine the need for supplementary acquisition,which can lead to delays in the acquisition process.The implementation of real-time monitoring and early warning systems for equipment health status aims to mitigate the risk of poor data quality resulting from equipment anomalies.Furthermore,the real-time synchronous transmission between the cloud and server addresses the bottleneck of slow download speeds associated with the centralized retrieval of data from multiple node devices during blind acquisition and storage.A real-time microseismic data acquisition test and verifi cation were conducted at a fracturing site in an eastern oil and gas fi eld.Analysis of the test data indicates that the overall performance indicators of the system are comparable to those of existing mainstream system equipment,demonstrating stability and reliability.The performance parameters fully satisfy the technical requirements for oilfield fracturing monitoring scenarios,suggesting promising prospects for further promotion and application.
文摘This paper presents an investigation of the tribological performance of AA2024–B_(4)C composites,with a specific focus on the influence of reinforcement and processing parameters.In this study three input parameters were varied:B_(4)C weight percentage,milling time,and normal load,to evaluate their effects on two output parameters:wear loss and the coefficient of friction.AA2024 alloy was used as the matrix alloy,while B_(4)C particles were used as reinforcement.Due to the high hardness and wear resistance of B_(4)C,the optimized composite shows strong potential for use in aerospace structural elements and automotive brake components.The optimisation of tribological behaviour was conducted using a Taguchi-Grey Relational Analysis(Taguchi-GRA)and the Technique for Order of Preference by Similarity to Ideal Solution(TOPSIS).A total of 27 combinations of input parameters were analysed,varying the B_(4)C content(0,10,and 15 wt.%),milling time(0,15,and 25 h),and normal load(1,5,and 10 N).Wear loss and the coefficient of friction were numerically evaluated and selected as criteria for optimisation.Artificial Neural Networks(ANNs)were also applied for two outputs simultaneously.TOPSIS identified Alternative 1 as the optimal solution,confirming the results obtained using the Taguchi Grey method.The optimal condition obtained(10 wt.%B_(4)C,25 h milling time,10 N load)resulted in a minimum wear loss of 1.7 mg and a coefficient of friction of 0.176,confirming significant enhancement in tribological behaviour.Based on the results,both the B_(4)C content and the applied processing conditions have a significant impact on wear loss and frictional properties.This approach demonstrates high reliability and confidence,enabling the design of future composite materials with optimal properties for specific applications.
基金supported by the Key Technologies R&D Program of Xiamen(No.3502Z20231057)Industry Leading Key Projects of Fujian Province(No.2022H0057)+2 种基金the National Natural Science Foundation of China(No.21975212)High-Level Talent Start-Up Foundation of Xiamen Institute of Technology for financial support(No.YKJ23017R)Graduate Science and Technology Innovation Program of Xiamen University of Technology(No.YKJCX2023194).
文摘LiMnxFe1-xPO_(4) is a promising cathode candidate due to its high security and the availability of a high 4.1 V operating voltage and high energy density.However,the poor electrochemical kinetics and structural instability currently hinder its broader application.Herein,inspired by the hydrogen-bonded cross-linking and steric hindrance effect between short-chain polymer molecules(polyethylene glycol-400,PEG-400),the pomegranate-type LiMn_(0.5)Fe_(0.5)PO_(4)-0.5@C(P-LMFP@C)cathode materials with 3D ion/electron dual-conductive network structure were constructed through ball mill-assisted spray-drying method.The intermolecular effects of PEG-400 promote the spheroidization and uniform PEG coating of LMFP precursor,which prevents agglomeration during sintering.The 3D ion/electron dual-conductive network structure in P-LMFP@C accelerates the Li^(+)transport kinetics,improving the rate performance and cycling stability.As a result,the designed P-LMFP@C has remarkable electrochemical behavior,boasting excellent capacity retention(98%after 100 cycles at the 1C rate)and rate capability(91 mAh·g^(-1)at 20C).Such strategy introduces a novel window for designing high-performance olivine cathodes and offers compatibility with a range of energy storage materials for diverse applications.
