Earthworms exhibit distinct biological traits contributing to ecosystem resilience.As a keystone species of terrestrial ecosystem,they influence soil health.This study investigates the transformation of bacterial popu...Earthworms exhibit distinct biological traits contributing to ecosystem resilience.As a keystone species of terrestrial ecosystem,they influence soil health.This study investigates the transformation of bacterial population as they pass through the gut of Metaphire birmanica,with a focus on knowing its role in shaping microbial diversity,directing to understand its influence on soil fertility and ecosystem restoration.We used advance high-throughput 16S rRNA sequencing to assess surrounding soil,earthworm gut,and cast samples.Following DNA extraction,the process of amplification,and sequencing,bacterial taxonomy was compared across various levels using bioinformatics.Findings revealed that M.birmanica gut acted as a selective environment,leading a distinct microbial composition that predominantly favored anaerobic bacteria like Firmicutes.The observed microbial patterns suggest potential ecological implications,including possible roles of families like Sphingomonadaceae and Pseudomonadaceae in nutrient cycling and Bacillus and Pseudomonas in plant growth promotion,increased nutrient availability,and enhanced soil structure.These results highlight M.birmanica's significant role in influencing soil microbial ecology,offering significant implications for sustainable agriculture and soil restoration.展开更多
文摘Earthworms exhibit distinct biological traits contributing to ecosystem resilience.As a keystone species of terrestrial ecosystem,they influence soil health.This study investigates the transformation of bacterial population as they pass through the gut of Metaphire birmanica,with a focus on knowing its role in shaping microbial diversity,directing to understand its influence on soil fertility and ecosystem restoration.We used advance high-throughput 16S rRNA sequencing to assess surrounding soil,earthworm gut,and cast samples.Following DNA extraction,the process of amplification,and sequencing,bacterial taxonomy was compared across various levels using bioinformatics.Findings revealed that M.birmanica gut acted as a selective environment,leading a distinct microbial composition that predominantly favored anaerobic bacteria like Firmicutes.The observed microbial patterns suggest potential ecological implications,including possible roles of families like Sphingomonadaceae and Pseudomonadaceae in nutrient cycling and Bacillus and Pseudomonas in plant growth promotion,increased nutrient availability,and enhanced soil structure.These results highlight M.birmanica's significant role in influencing soil microbial ecology,offering significant implications for sustainable agriculture and soil restoration.
