Cucumber is one of the most widely consumed vegetables worldwide,and the fruit spine is an important fruit quality trait.Expansins play critical roles in fruit development;however,the regulation of expansins in cucumb...Cucumber is one of the most widely consumed vegetables worldwide,and the fruit spine is an important fruit quality trait.Expansins play critical roles in fruit development;however,the regulation of expansins in cucumber fruit spine development has not been reported.In this study,33 expansin genes were identified in the cucumber genome V3;additionally,expansin genes in Citrullus lanatus,Cucumis melo,Cucurbita maxima,Lagenaria siceraria,and Benincasa hispida were also identified.Phylogenetic analysis of expansin proteins in Cucurbitaceae and Arabidopsis showed that they evolved separately in each plant species.Phylogenetic analysis showed that C.maxima was derived earlier than the other five Cucurbitaceae species.The expression of CsEXPA2,CsEXPA14,and CsEXLA3 varied in cucumber lines with different fruit spine densities.A yeast two-hybrid assay showed that a putative auxin transporter encoded by numerous spine gene(ns)interacts with CsEXLA2,which may be involved in the development of the numerous spines in cucumber.These results provide novel insights into the expansins related to plant development and fruit spine development in cucumber.展开更多
Goss ypium barbadense L.is one of the most valuable cotton species due to its silkiness,luster,long staple,and high strength.Transferring the excellent fiber trait s from G.barba dense as the sec-ondary gene pool to t...Goss ypium barbadense L.is one of the most valuable cotton species due to its silkiness,luster,long staple,and high strength.Transferring the excellent fiber trait s from G.barba dense as the sec-ondary gene pool to the widely cultivated G.hirsutum via traditional and molecularaided selection is an attractive aim of breeders.So identification of t he novel genes functions from the G.barba dense fi-ber is important.Expansins are plant cell wall proteins first discovered in studies of plant cell enlarge-ment,and they have unique"loosening"effects on plant cell walls.They should be very important in the elongating fiber.展开更多
Background Sustainable livestock production is essential for food security and environmental management. Lignocellulosic biomass can be used in animal feed, thereby reducing feed production costs and enhancing sustain...Background Sustainable livestock production is essential for food security and environmental management. Lignocellulosic biomass can be used in animal feed, thereby reducing feed production costs and enhancing sustainability. Expansin-like proteins(ELPs) play essential roles in plant cell wall degradation, yet their functions remain largely underexplored in rumen microbes. The purpose of this study was to investigate the effects of rumen microbial ELPs on lignocellulose degradation.Results This study systematically identified 396 ELPs within the rumen microbiota, uncovering remarkable diversity, particularly among anaerobic fungi. Three representative ELPs from Pecoramyces ruminantium F1(PFLoos_1, PFSWO1_1, PFSWO2_1) were selected for biochemical characterization. While PFSWO2_1 could not be expressed, PFLoos_1 and PFSWO1_1 exhibited significant synergy with cellulases. The CBM10-containing PFSWO1_1 demonstrated superior thermal stability(up to 65 ℃) and substrate affinity, increasing rice straw hydrolysis efficiency by 21.6%(reducing sugar yield) compared to cellulase alone. Structural analyses revealed that CBM10 enabled PFSWO1_1 to preferentially bind complex substrates, whereas the single-domain PFLoos_1 targeted simpler substrates. Notably, ELP pretreatment of corn stover significantly improved fermentation quality(pH and lactic acid) and nutritional value(neutral detergent fiber, acid detergent fiber, and water-soluble carbohydrates).Conclusions These findings indicate that ELPs are abundant in the rumen and play a synergistic role in lignocellulosic biomass conversion.展开更多
基金supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-23)the National Natural Science Foundation of China (31672172)+1 种基金The Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS)the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs,China。
文摘Cucumber is one of the most widely consumed vegetables worldwide,and the fruit spine is an important fruit quality trait.Expansins play critical roles in fruit development;however,the regulation of expansins in cucumber fruit spine development has not been reported.In this study,33 expansin genes were identified in the cucumber genome V3;additionally,expansin genes in Citrullus lanatus,Cucumis melo,Cucurbita maxima,Lagenaria siceraria,and Benincasa hispida were also identified.Phylogenetic analysis of expansin proteins in Cucurbitaceae and Arabidopsis showed that they evolved separately in each plant species.Phylogenetic analysis showed that C.maxima was derived earlier than the other five Cucurbitaceae species.The expression of CsEXPA2,CsEXPA14,and CsEXLA3 varied in cucumber lines with different fruit spine densities.A yeast two-hybrid assay showed that a putative auxin transporter encoded by numerous spine gene(ns)interacts with CsEXLA2,which may be involved in the development of the numerous spines in cucumber.These results provide novel insights into the expansins related to plant development and fruit spine development in cucumber.
文摘Goss ypium barbadense L.is one of the most valuable cotton species due to its silkiness,luster,long staple,and high strength.Transferring the excellent fiber trait s from G.barba dense as the sec-ondary gene pool to the widely cultivated G.hirsutum via traditional and molecularaided selection is an attractive aim of breeders.So identification of t he novel genes functions from the G.barba dense fi-ber is important.Expansins are plant cell wall proteins first discovered in studies of plant cell enlarge-ment,and they have unique"loosening"effects on plant cell walls.They should be very important in the elongating fiber.
基金funded by the National Natural Science Foundation of China(grant nos.32372905)。
文摘Background Sustainable livestock production is essential for food security and environmental management. Lignocellulosic biomass can be used in animal feed, thereby reducing feed production costs and enhancing sustainability. Expansin-like proteins(ELPs) play essential roles in plant cell wall degradation, yet their functions remain largely underexplored in rumen microbes. The purpose of this study was to investigate the effects of rumen microbial ELPs on lignocellulose degradation.Results This study systematically identified 396 ELPs within the rumen microbiota, uncovering remarkable diversity, particularly among anaerobic fungi. Three representative ELPs from Pecoramyces ruminantium F1(PFLoos_1, PFSWO1_1, PFSWO2_1) were selected for biochemical characterization. While PFSWO2_1 could not be expressed, PFLoos_1 and PFSWO1_1 exhibited significant synergy with cellulases. The CBM10-containing PFSWO1_1 demonstrated superior thermal stability(up to 65 ℃) and substrate affinity, increasing rice straw hydrolysis efficiency by 21.6%(reducing sugar yield) compared to cellulase alone. Structural analyses revealed that CBM10 enabled PFSWO1_1 to preferentially bind complex substrates, whereas the single-domain PFLoos_1 targeted simpler substrates. Notably, ELP pretreatment of corn stover significantly improved fermentation quality(pH and lactic acid) and nutritional value(neutral detergent fiber, acid detergent fiber, and water-soluble carbohydrates).Conclusions These findings indicate that ELPs are abundant in the rumen and play a synergistic role in lignocellulosic biomass conversion.
