Objective: To assess the performances of Cobas 6000 e601 and EVOLIS Bio Rad in the detection of HIV, HBV and HCV in blood donors in Libreville(Gabon).Methods: A cross-sectional investigation was conducted in July 2017...Objective: To assess the performances of Cobas 6000 e601 and EVOLIS Bio Rad in the detection of HIV, HBV and HCV in blood donors in Libreville(Gabon).Methods: A cross-sectional investigation was conducted in July 2017 in a total of 2 000 blood donors recruited at the National Blood transfusion Center, Libreville Gabon.Among them, 363 donors were selected to compare the performances of COBAS 6000 e601(electro-chemiluminescence) and EVOLIS Bio Rad in detecting HIV, HBV and HCV using Cohen's kappa coefficient.Results: Both methods yielded similar results for the detection of HIV and HBs Ag. A very good agreement of 93.39% and an excellent agreement of 98.90% were obtained for the detection of HIV and Hbs Ag, with kappa values of 0.80 and 0.98, respectively. The observed agreement of 91.86% was found for the detection of HCV, which gave a fair agreement between the two methods with kappa = 0.33.Conclusions: The two evaluation methods showed a similar performance in the detection of HIV, HBV. However, given the high rate of intra and inter-genotypes recombination known for HIV and HBV, more robust techniques of detection such as polymerase chain reaction should be used to prevent post-transfusion contaminations.展开更多
基金supported by the National blood transfusion center through the Progamme de Support Recherche No.2
文摘Objective: To assess the performances of Cobas 6000 e601 and EVOLIS Bio Rad in the detection of HIV, HBV and HCV in blood donors in Libreville(Gabon).Methods: A cross-sectional investigation was conducted in July 2017 in a total of 2 000 blood donors recruited at the National Blood transfusion Center, Libreville Gabon.Among them, 363 donors were selected to compare the performances of COBAS 6000 e601(electro-chemiluminescence) and EVOLIS Bio Rad in detecting HIV, HBV and HCV using Cohen's kappa coefficient.Results: Both methods yielded similar results for the detection of HIV and HBs Ag. A very good agreement of 93.39% and an excellent agreement of 98.90% were obtained for the detection of HIV and Hbs Ag, with kappa values of 0.80 and 0.98, respectively. The observed agreement of 91.86% was found for the detection of HCV, which gave a fair agreement between the two methods with kappa = 0.33.Conclusions: The two evaluation methods showed a similar performance in the detection of HIV, HBV. However, given the high rate of intra and inter-genotypes recombination known for HIV and HBV, more robust techniques of detection such as polymerase chain reaction should be used to prevent post-transfusion contaminations.
