[Objective]The aim was to obtain effective microsatellite markers from the public strawberry ESTs data.[Method]The distribution frequency and density of simple sequence repeats（SSRs） in strawberry EST was analyzed b...[Objective]The aim was to obtain effective microsatellite markers from the public strawberry ESTs data.[Method]The distribution frequency and density of simple sequence repeats（SSRs） in strawberry EST was analyzed by MISA（Microsatellite） software,and the redundancy was analyzed with CAP3 software.[Result]A total of 10 129 SSR sequence was received in 17 565 of EST sequences,and the distance among SSRs was about 0.90 kb,in which the Hexanucleotide repeats gained the greatest abundance,which was accounted for 61.0%,while the Trimeric,Monomeric,Dimeric,Tetrameric and Pentameric repeats accounted for 14.3%,13.1%,6.2%,4.3% and 1.1% respectively.The most abundant motif was A/T,AG,AAG and AAAG in the Monomeric,Dimeric,Trimeric and Tetrameric repeat motifs,while the CG was not found in the coding region.In these six types of repeat motifs,there was no significant difference between redundant and non redundant ESTs.[Conclusion]The availability of microsatellite markers could be expected to be achieved in the public strawberry database.展开更多
基金The National Basic Research Program of China(200611D100203)The Program for Changjiang Scholars and Innovative Research Teamin University,Ministry of Education of China(200558)+2 种基金The Cultivation Fund of the Key Scientific and Technical Innovation Project,Ministry of Education of China(2004-295)The National Nature Science Foundation(30671350)111 project(B07049)
基金Supported by the National Natural Science Foundation of China(30370743)Foundation of Jiangxi Normal University (2441,2732)~~
文摘[Objective]The aim was to obtain effective microsatellite markers from the public strawberry ESTs data.[Method]The distribution frequency and density of simple sequence repeats（SSRs） in strawberry EST was analyzed by MISA（Microsatellite） software,and the redundancy was analyzed with CAP3 software.[Result]A total of 10 129 SSR sequence was received in 17 565 of EST sequences,and the distance among SSRs was about 0.90 kb,in which the Hexanucleotide repeats gained the greatest abundance,which was accounted for 61.0%,while the Trimeric,Monomeric,Dimeric,Tetrameric and Pentameric repeats accounted for 14.3%,13.1%,6.2%,4.3% and 1.1% respectively.The most abundant motif was A/T,AG,AAG and AAAG in the Monomeric,Dimeric,Trimeric and Tetrameric repeat motifs,while the CG was not found in the coding region.In these six types of repeat motifs,there was no significant difference between redundant and non redundant ESTs.[Conclusion]The availability of microsatellite markers could be expected to be achieved in the public strawberry database.
